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All Journal Buletin Peternakan Jurnal Veteriner Animal Agricultural Journal Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Animal Production : Indonesian Journal of Animal Production JEPA (Jurnal Ekonomi Pertanian dan Agribisnis) Livestock and Animal Research
Yon Soepri Ondho
Department Of Animal Science, Faculty Of Animal And Agricultural Sciences, Diponegoro University, Semarang, 50275, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Environmental Science Social Sciences Veterinary Other

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Journal : Jurnal Veteriner

 1 
Polimorfisme Protein Serum Darah Induk Sapi Beranak Kembar dan Tunggal pada Sapi Peranakan Ongole dan Keturunan Simental (BLOOD SERUM PROTEIN POLYMORPHISM OF THE COW DELIVERED TWIN OR SINGLE CALVES IN ONGOLE GRADE AND SIMENTAL CROSSBRED) Tri Yuwono; Irene Sumeidiana; Yon Soepri Ondho; Edy Kurnianto
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (118.786 KB)

Abstract

The twinning cow is presumable have difference in blood protein typology with the single cow. Thestudy investigated blood protein polymorphism in twinning and single of ongole grade and Simmentalcrossbred cows. The study used four twinning versus eight single ongole grade and seven twinning versussix single Simmental crossbred cows. Blood samples were analyzed by using marker ExactPro BroadRange (10-245kDa) and Sodium Dodecyl Sulphate-polyacrilamide Gel Electrophoresis method, to estimatethe loci of albumin (Alb), post-albumin (Pa), ceruloplasmin (Cp), transpherrin (Tf), and amylase-I (Amy-I).The genetic variation was analyzed based on the value of gene frequency, individual heterozigosity andaverage heterozigosity. In this study the twinning ongole grade cows had lower average heterozigosity(0.46) than the single ongole grade cows (0.49). The twinning Simmental crossbred cows had lower averageheterozigosity (0.35) than the single Simmental crossbred cows (0.40). The average heterozigosity ofongole grade cows had higher value than Simmental crossbred cows. The average heterozigosity rangedfrom 0.35-0.49, which was indicated high genetic variation in all cows. Higher gene frequency of allelealbumin A than albumin B influences the twinning trait in ongole grade and Simmental crossbred cows.
In Vitro Ovine Embryo Culture: Effectiveness of TCM-199 in Combination with 17?-Estradiol Hormone and Co-Culture of FTEC Yon Soepri Ondho; Dela Ayu Lestari; Daud Samsudewa
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (147.646 KB) | DOI: 10.19087/jveteriner.2020.21.4.558

Abstract

Objective of this research was to determine the effectiveness of various culture mediums on thecleavage to morula in in vitro embryo culture of ovine. A total of 1.064 zygotes (fertilized oocytes) were grown in three kinds of different embryo culture medium, which were Tissue Culture Medium 199 (TCM-199) (T1), TCM-199 + 17รข-estradiol (T2) and TCM-199 + co-culture of Fallopian Tube Epithelial Cell (FTEC) (T3). Data were analyzed using a completely randomized design with ANOVA analysis and HonestlySignificant Difference test. The variable observed were the percentage of embryo cleavage and morula. Results showed that T3 had the highest percentage (72.45%) of the cleavage occurred in zygotes cultured in the TCM-199 + FTEC co-culture medium, as well as embryo growth to the morula stage (75.75%). Incontrast, zygotes cultured in T1 and T2 had lower cleavage and lower morula development than T3, as much as 27.38%; 47.82% and 58.15%; 66.66%, respectively. In conclusion, TCM-199 + FTEC co-culture medium is the best medium for embryo culture.
Co-Authors Afdela, Alfi Agung Subrata Arnia Septarosa Dewi Arry Diwityas Tuhu Bambang Waluyo Hadi Eko Prasetiyono C. M. Sri Lestari Daud Samsudewa Daud Samsudewa Dela Ayu Lestari, Dela Ayu Desyandri Desyandri Edjeng Suprijatna Edy Kurnianto Edy Kurnianto Enny Tantini Setiatin Hendhita Reski Wahyutea, Hendhita Reski Hilkias, Welda Irene Sumeidiana Isna Sa'adah Mentari, Fausta Krisna Mukson Mukson Muslichatun Nisa Mutiara Briliana Zahra Pramesti Kusuma Pratiwi Rita Purwaningsih Sally Saurma Silalahi Sismala Widitania sutiyono sutiyono Sutiyono Sutiyono Sutopo Sutopo Tertyani Kartika Pratiwi Tri Yuwono Trisna Itmamulwafa Widiyanto Widiyanto Widiyanto Widiyanto Widodo, Hermawan Setyo