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Perbandingan Pemberian Anestesi Kombinasi Ketamin-Xilazin dengan Anestesi Propofol Terhadap Gambaran Elektrokardiogram pada Kucing Domestik Saputri, Aditya Dwi; Ikliptikawati, Dini Kurnia; Rell, Fedri
Indonesia Medicus Veterinus Vol 9 (4) 2020
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/imv.2020.9.4.488

Abstract

Anestesi merupakan kondisi yang dikendalikan dengan ketidaksadaran yang bersifat reversible. Tujuan penelitian ini adalah untuk mengetahui perbandingan pemberian anestesi ketamin-xilazin dengan anestesi propofol terhadap gambaran elektrokardiogram (EKG) pada kucing domestik. Sebanyak 15 ekor kucing domestik berumur 1.5-3 tahun dan sehat. Kucing dibagi kedalam tiga kelompok yaitu kelompok kontrol (KK), kelompok perlakuan I (KP1), dan kelompok perlakuan 2 (KP2). Anestesi propofol diberikan pada KP1, kombinasi ketamin-xilazin diberikan pada KP2, dan tanpa pemberian anestesi pada KK. Pengambilan data EKG yang meliputi heart rate, durasi P, durasi QRS, durasi T, interval PR, interval QT, Amplitudo P dan Amplitudo R, serta MEA (mean electrical axis) pada kelompok perlakuan diambil pada stadium III. Kelompok KP1 memiliki nilai parameter yang lebih mendekati nilai normal daripada KP2. Berdasarkan hasil penelitian ini disimpulkan bahwa penggunaan anestesi propofol lebih aman daripada anestesi kombinasi ketamin-xilazin terhadap gambaran EKG jantung
Administration of Live-Attenuated Newcastle Disease (ND) Vaccines Derived from B1 and LaSota Strain and Their Effect on Broiler Antibody Titers Andi Magfirah Satya Apada; A. Rianti Rhasinta Alifha; Fedri Rell; Trini Purnamasari Syahid
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 6 No.1, January 2022
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v6i1.19400

Abstract

Newcastle Disease is a contagious disease caused by Avian Paramyxovirus and infects different types of poultries including broiler. Vaccination as a preventive effort against the ND virus could employ both attenuated and inactivated vaccines. This study aims to identify the effect of administering live-attenuated LaSota and B1 ND vaccines against broiler antibody titers. This experiment employed 54 DOCs and was divided into 3 groups of treatments. Vaccination was performed at the age of 3 days old through eye drop administration. Blood specimens were taken from vena brachialis at the age of 7, 14, 21 days old. Hemagglutination Inhibition (HI) assay was analyzed using One-Way Analysis of Variance (ANOVA) followed by Least Significant Different (LSD) test if the probability is significant (P<0.05). The data of Immune Percentage is presented using descriptive quantitative analysis.   The research results demonstrated that live-attenuated LaSota ND vaccination at the age of 3 days could sustain and induce immunity until the age of 21 days while B1 ND vaccination at the age of 3 days could only sustain immune protection until the age of 14 days. Live-attenuated LaSota and B1 ND vaccines did not have any significant effect on the broiler antibody titers.   Keywords: Broiler, Newcastle Disease (ND), LaSota vaccine, B1 vaccine, Hemagglutination test, Hemagglutination Inhibition Test
UJI AKTIVITAS EKSTRAK ETANOL KULIT PISANG MAS (Musa acuminata (AA Goup)) TERHADAP PENURUNAN KADAR GLUKOSA DARAH MENCIT (Mus Musculus) YANG DIINDUKSI ALOKSAN Kadek Evi D.P. Dewi; Abdul Wahid Jamaluddin; Fedri Rell
As-Syifaa Jurnal Farmasi Vol 10, No 2 (2018): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (273.298 KB) | DOI: 10.33096/jifa.v10i2.342

Abstract

Diabetes mellitus (DM) is a metabolic diseases caused by impaired insulin secretion. The prevalence of DM in pets increased from 2006 until 2015.Therapy by using insulin feared can be worse for patient, so yhat alternative medicine is needed like banana peel. The aims of this study isto determine the effect ofethanol extract of Mas banana peel (Musa acuminata (AA Group)) to decrease level of glucose in mice’s blood (Mus musculus) were induced by alloxan. This research was conducted in June 2017 at Biopharmaceutical Laboratory Faculty of Pharmacy, Hasanuddin University. This study uses 24 male mice which divided into 6 groups where the normal control (standard diet), negative control (Na.Cmc 1%), positive control (Metformin 140mg/KgBB) and treatment group by ethanol extract of Mas banana peel 1%, 5%, and 25%. The treatment was given for 10 days. Data were analyzed using one way Anova. The result of this study showed that ethanol extract in banana peel decrease the level of blood glucose which the decrease percentage are 43,15%, 54,61%, 74,28%. The summary of this research is the treatment by using variant concentration of ethanol extract of Mas banana peelfor 10 days give the significant effect for decreasing the level of blood glucose (p<0,05) and the activity of ethanol extract of Mas Banana Peel 25% are not give the significant effect with metformin dose 140mg/KgBB (p>0,05).
Deteksi Logam Kadmium (Cd) dan Timbel (Pb) pada Sapi Peranakan Ongole yang Digembalakan di Tempat Pembuangan Akhir (TPA) Tamangapa Makassar Fedri Rell
Jurnal Ilmu dan Industri Peternakan Vol 7 No 1 (2021): JUNI
Publisher : State Islamic University (UIN) Alauddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/jiip.v7i1.10095

Abstract

Sapi peranakan ongole adalah salah satu sapi penghasil daging. Logam berat Cd dan Pb adalah logam beracun bagi makhluk hidup. Penelitian bertujuan untuk mendeteksi adanya logam kadmium (Cd) dan timbel (Pb) pada sapi peranakan ongole yang digembalakan di Tempat Pembuangan Akhir (TPA) Tamangapa Makassar. Penelitian dilakukan pada bulan Mei sampai Juni 2019 di Laboratorium Kimia Makanan Ternak, Departemen Nutrisi dan Makanan Ternak  Fakultas Peternakan Universitas Hasanuddin. Sampel sebanyak 15 sampel darah dari lima lokasi peternakan. Pengukuran kadar Cd dan Pb dalam darah menggunakan metode uji Atomic Absorption Spectrophotometer (AAS). Hasil dari penelitian menunjukkan keberadaan logam berat kadmium yang berada dikisaran antara 0,290 ppm - 1,008 ppm dan timbel yang berada  dikisaran antara 1,32 ppm – 9,91 ppm. Kesimpulan dari penelitian adalah terdeteksinya logam kadmium dan timbel pada sapi peranakan ongole yang digembalakan di Tempat Pembuangan Akhir (TPA) Tamangapa Makassar. 
Identifikasi Salmonella pullorum pada Ayam Petelur Periode Grower dengan Uji Aglutinasi dan Makroskopik di Peternakan Ayam Kabupaten Sidrap Genna Prama Nugroho; Andi Magfira Satya Apada; Fedri Rell
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 8, No 3 (2021): JITRO, September 2021
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (830.82 KB) | DOI: 10.33772/jitro.v8i3.12338

Abstract

ABSTRAKPenyakit pullorum merupakan penyakit yang sering menginfeksi ayam yang disebabkan oleh Salmonella pullorum. Penelitian ini bertujuan untuk mengidentifikasi ada tidaknya penyakit pullorum pada ayam petelur periode grower di peternakan ayam kabupaten Sidrap. Pengambilan sampel menggunakan metode purposive sampling. Sampel serum dan organ diambil dari 19 peternakan ayam di Batu-Batu. Sebanyak 57 sampel serum diuji dengan uji aglutinasi. Diperoleh sebanyak 26 sampel serum yang positif dan dilanjutkan dengan pemeriksaan makroskopik. Pemeriksaan makroskopik ditemukan adanya hepatomegali, lesi berwarna kuning-pucat, konsistensi lunak, dan nodul-nodul di permukaan hati serta adanya kardomegali. Hasil penelitian ini menunjukkan bahwa peternakan ayam layer periode grower di kabupaten Sidrap telah terinfeksi Salmonella pullorum dangan gambaran patologis yang bervariasi.  Kata kunci: ayam petelur, identifikasi, Salmonella pullorum, makroskopikIdentification of Salmonella Pullorum in Grower Period of Laying Hens By Agglutinated and Macroscopic Test in Layer Farms of Sidrap RegencyABSTRACTPullorum is a bacterial disease that commonly infected the chicken infected by Salmonella pullorum. This research aimed to identify Salmonella pullorum in the growing period of laying hen in Batu-Batu. The research used a purposive sampling method. The organ and serum samples were collected from 19 farms. Agglutination test for serum and macroscopic test for organ was performed. There were 26 samples that tested positive from 57 serum samples. There were 26 organ samples of macroscopic treatment that resulted in liver damages in the forms of swelling, pale yellow lesions, soft consistency, and nodules on the surface. The heart also experienced changes through swelling, soft consistency, uneven surface, and a mixture of fluid and fibrin inside the pericardium. It can be concluded that layer farms in Sidrap districts have been infected by Salmonella pullorum affecting the growing period.Keywords : laying hens, identification, Salmonella pullorum, macroscopic
Virulensi Virus Newcastle Disease Isolat Lapang Berdasarkan Analisis Bioinformatika Gen Protein Hemaglutinin - Neuraminidase Fedry Rell; Anak Agung Mirah Adi; I Gusti Ngurah Kade Mahardika
Veterinary Science and Medicine Journal Vol 3 No 1 (2015)
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (913.341 KB)

Abstract

Newcastle disease virus (NDV) is very contagious disease agent, and causes many outbreaks trough out thecountry of Indonesia. This study was conducted to determine the virulence of Bali field isolate of NDVbased on haemaglutinin-neuraminidase (HN) protein genes. Four field isolates from different locationswere propagated at fertilized chicken eggs of 9 days old. Allantoic fluid was harvested and NDV wasconfirmed using standard haemaglutination (HA) and haemaglutination inhibition test (HI) methods. Thefragment of HN protein gene was amplified using RT-PCR. The product was sequenced using Big-Dyetermination method. All four isolates grew well with the titer of 2[5]-2[9] HA unit and could be confirmedusing HI test. The HN genes, however, exhibited variations at its 3-D structure and hydrophobicity betweenthe virus that previously circulated in Indonesia and vaccine virus. It is concluded that all four Bali’sisolates under this study are virulent VND of the genotype VII. Further testing is needed to justify the bestformula of NDV vaccine to be used trough out Indonesia.
Polimorfisme Lokus Mikrosatelit D10S1432 pada Populasi Monyet Ekor Panjang Di Sangeh F. Rell; S.K. Widyastuti; I N. Wandia
Veterinary Science and Medicine Journal Vol. 1 No. 1 2013
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (606.647 KB)

Abstract

Genetic polymorphisms are variations in the genetic structure of populations that represents the base level of biodiversities.  This research aimed to assess the polymorphism of microsatellite locus of D10S1432 in the population of long-tailed macaques in Sangeh, Bali.  A total of 18 blood samples collected as a source of DNA. DNA was extracted using QIAmp® DNA Blood Mini Kit. Microsatellite locus was amplified by polymerase chain reaction technique (PCR) with a total of 30 cycles, and a 57oC of annealing temperature. Furthermore, the alleles were separated by 7% polyacrylamide gel electrophoresis, and emerged with silver staining. The results showed that there were three types of alleles found at D10S1432 locus in the long-tailed macaques population in Sangeh. The alleles size varied from 170 bp to 186 bp. The allele frequencies were  0.28, 0.67, and 0.05 for allele 170, allele 174, and allele 186 consecutively. The heterozygosity of the locus was 0.48. It can be concluded that microsatellites locus D10S1432 is polymorphic in the long-tailed macaques population in Sangeh.
Analisis Filogeni Virus Newcastle Disease Isolat Bali Tahun 2013 Sampai 2014 Berdasarkan Sekuen Daerah Pemotongan Protein Fusion Fedri Rell; Anak Agung Ayu Mirah Adi; I Gusti Ngurah Kade Mahardika
Buletin Veteriner Udayana Vol. 13 No. 1 Pebruari 2021
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (437.757 KB) | DOI: 10.24843/bulvet.2021.v13.i01.p11

Abstract

Newcastle disease is a contagious disease caused by the Avian paramyxovirus serotype 1 virus, which infected the poultry. The aims of this study were conducted to phylogenetic analysis of the fusion protein cleavage site of Newcastle disease virus found in a chicken farm in the province of Bali throughout the year 2013 to 2014. There are four isolates from sick chicken’s cases/death suspected of being infected by the Newcastle disease virus. The sequence of the protein fusion was aligned and edited using MEGA5. The amino acid sequence of the F cleavage site of all isolates was 112R-R-Q-K-R-F117 and analysis of phylogeny tree, which justified that all four are virulent of Newcastle disease virus. It is concluded that four Bali isolates in 2013 to 2014 under this study are Newcastle disease virus of genotype VII.
Mapping of the Coronavirus Circulating in Asia Based on Sequence of Gene Spike and Membrane Protein Used MEGA-X Aplication Fedri Rell; Muhammad Fikri Raditya Jalil; Anggini Putri Husada; Nur Azizah Dariming
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 6 No.1, January 2022
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v6i1.17952

Abstract

Coronavirus are viruses that can be transmitted to human and animals. Severe acute respiratory syndrome, middle east respiratory syndrome, and Coronavirus disease 2019 are disease can be caused by several subtypes of coronavirus.  The aims of this study were to mapping of the coronavirus circulating in Asia based on sequence of gene spike and membrane protein virus. Totally of 67 coronavirus spike protein and membrane gene sequence were accessed via GenBank® (www.ncbi.nlm.nih.gov/genbank/) matched with the ClustalW Method MEGA-X. The result of the study are 20 groups of coronavirus were found based on spike protein gene sequences and 27 groups of coronavirus were found based on membrane protein gene sequences which were different with the first group of coronavirus found in Wuhan. Therefore, it can be concluded that the coronavirus circulate in several Asian countries had been mutate on gene spike and membrane protein. Keywords: Asia, Coronavirus, MEGA-X, Membrane Protein, Spike Protein
Identification of Yersinia spp in Catfish (Claria spp) Attacked Enteric Redmouth Disease at the Education Veterinary Clinic, Hasanuddin University Fedri Rell; Dwi Kesuma Sari; Muhammad Fadhlullah Mursalim; Baso Yusuf; Andi Magfira Satya Apada; Astri Caturutami Sjahid
Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) VOLUME 6 No 2, JULY 2022
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/jrvi.v6i2.21091

Abstract

Catfish is one of the fish that has a high economic value so that it becomes one of the export commodities to meet the consumption and protein needs of the community so that it is widely cultivated. In cultivation, catfish can be attacked by various diseases, one of which can be caused by bacteria. The bacteria that often cause death in fish, both marine and freshwater fish, is Yersinia spp. Yersinia spp is a group of gram-negative bacteria, in the form of bacilli and motile and is also reported to be one of the pathogenic diseases that often attack fish. samples were taken on August 12, 2021 from the mouth organs of catfish at the Hasanuddin University Education Animal Clinic. Samples were cultured in Tryptic Soy Agar (TSA) for 24 hours and then purified in Mac Conkey Agar (MCA). The culture results showed the presence of bacterial growth. Follow-up examination using a microscope and showed the presence of red (Gram negative) and bacilli-shaped bacteria.