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All Journal Agrotrop : Journal on Agriculture Science Buletin Udayana Mengabdi Journal of Agricultural Science and Biotechnology E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Indonesian Journal of Mathematics and Natural Sciences Advances in Tropical Biodiversity and Environmental Sciences
I Ketut Siadi
Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana, Denpasar
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Education Environmental Science Mathematics Veterinary

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Toleransi Penundaan Prosesing Terhadap Mutu Fisik dan Mutu Fisiologis Benih Kedelai (Glycine max L. Merril) ROBERTO BOBHOPE ARUAN; I DEWA NYOMAN NYANA; I KETUT SIADI; I GUSTI NGURAH RAKA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.7, No.2, April 2018
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Tolerance of Processing Delay to Physical and Physiological quality of Soya Bean (Glycine max L. Merril) The aim of this research is to figure out the time of the tolerance processing delay so that the physical quality and the physiological quality of soya bean seeds could be defended. This research was started in April 2017 at Jalan Cekomaria, Denpasar and also at the Lab. Pemuliaan Tanaman dan Teknologi Benih Faculty of Agriculture Udayana University. The design method was used for this research is a randomized complete block design (RCBD) using five treatment and five-time repetitions consist of W0= Without using process, W1= 1 day of Delaying process W2= 2 days of Delaying process, W3 = 3 days of Delaying process, W4 = 4 days of Delaying process. The statistic test and analyzing variance statistical test shows different result, the procedure followed by LSD method (Least Significance Different) with 95% of confidence level using costat program.The result of physical analysis test, shows that the role of the seeds which is without delaying process showing that the percentage of the seeds was 87,36% and the result of 4 days delaying process seeds are 11,256%. The result of the physiological test of the seeds using germinating test was, W0 (95,16%) and W4 (94,72%). The percentage of the vigor will be decreasing, which is W0 (80,52%) and W4 (34,4%). The equality control of the percentage was W0 (90,3%) and W4 (70,68%). Result of the observation of electrical conductivity showing that the lowest point of the electrical conductivity control is W0 (5,112 ? mhos cm¯² g¹) and the highest value of electrical conductivity was shown on the W4 (7,302 ? mhos cm¯² g¹).
Pengaruh Infeksi Beberapa Jenis Virus terhadap Penurunan Hasil pada Tanaman Cabai Rawit (Capsicum frutescens L.) I WAYAN SUKADA; I MADE SUDANA; I DEWA NYOMAN NYANA; GEDE SUASTIKA; KETUT SIADI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.3, Juli 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Effect of Infection of Some Viruses to the Decresing Yield on Rawit Pepper (Capsicum frutescens L.) This study aims to identify the types of viruses that infect the plants of chili pepper ( Capsicum frutescens L. ) in Kerta village, and to know the influence of some kind of viruses infection on yield decline. To verify the type of virus is done by taking a sample of the pepper plant leaf showing symptoms of the virus that has been demonstrated through serological and molecular tests. The results of this study indicate that the mosaic symptomatic chilli pepper plants were infected by some kind of virus such as CMV, TMV, and ChiVMV. Chilli pepper shows asymptomatic yellow were infected by the PepYLCV and chlorosis were infected by Polerovirus. The result of fruit chili harvest were showed the higher yields on the healthy plants with average 16.01 tonnes/ha, while the chilli that showed chlorosis symptomps were 8.42 tonnes/ha, and yellow symptomps were 3.07 tonnes/ha. The lowest were in the plants that shows mosaic symptomp with average 2.52 tonnes/ha. The virus infection caused the losses of yield of 47.40% in chlorosis symptomatic plants, 80.82% in the yellow symptomatic plant and 84.25%, in the mosaic symptomatic plants compared to healthy plants. High yield loss in the mosaic symptomatic plants were caused by the highest infection on chiilli pepper that infected by some kind of viruses.   Keywords : Chili pepper , mosaic, chlorosis, and yellow
Pengaruh Dry Heat Treatment dengan Penundaan Waktu Tanam Terhadap Pertumbuhan dan Hasil Tanaman Cabai Rawit (Capsicum Frutescens L.) I KADE DARMAWAN; I DEWA NYOMAN NYANA; I KETUT SIADI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.6, No.1, Januari 2017
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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The Effect of Dry Heat Treatment with Time Delay Planting on Plant Growth and Yield of Chili Pepper (Capsicum frutescens L.)Viral pathogens which cause disease is a major problem in reducing the production of chili pepper. The younger the plants are infected by the virus, the more severe symptoms of the disease caused. Based on this statement, it necessary to do a research that aims to determine the effect of Dry Heat Treatment with a delay time of planting on growth and yield of chili pepper. The research design used was a randomized block design with three treatments and nine replications. The treatments tested consists of Dry Heat Treatment with a delay time of planting (DHT + TT), Dry Heat Treatment (DHT), and control (NT). The growth variable and the yield that had been observed in these experiments were the plant height (cm), number of primary branches, leaf chlorophyll content, incidence of disease, and the yield (tons / ha). The results showed that Dry Heat Treatment with a delay time of planting (DHT + TT) provides better results in the cultivation of chili with 86.27 cm plant height increased by 106.83% compared with controls (NT), the number of branches 12.61 increased 41.53 % compared with controls (NT), and the yield 17.21 tons / ha increased by 256.87% compared with controls (NT).
Identifikasi Virus Yang Berasosiasi Dengan Penyakit Mosaik, Kuning, Dan Klorosis Pada Tanaman Cabai Rawit (Capsicum Frutescens L.) I GUSTI NGURAH BAGUS PRANATA PUTRA; NI MADE PUSPAWATI; I DEWA NYOMAN NYANA; I KETUT SIADI; GEDE SUASTIKA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.3, Juli 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Identification of virus that associated with Mosaic, Yellow, and Chlorosis disease on Chili Pepper (Capsicum frutescens L) This study aims to identify the types of viruses associated with mosaic, yellow, and chlorosis that infect the plants of chili pepper ( Capsicum frutescens L. ) in Kerta village, Gianyar. The method used is the ELISA (Enzyme Linked Immunosorbent Assay) and molecular techniques through PCR (Polymerase Chain Reaction). Research activities include (1) Survey endemic locations of viral diseases in Kerta village, Gianyar (2) Collecting leaf of chilli peper that shown, mosaic, yellowing and chlorosis (3) serology test by ELISA (4) molecular detection through PCR. The results show the percentage average of pepper plants showing mosaic symptoms (52.13%), yellow (22.75%), chlorosis (5.45%) and healthy plant (19.67%). Serology test by ELISA technique showed that the mosaic disease induced by the triple virus that is TMV, ChiVMV, and CMV, whereas yellow symptoms infected by PepYLCV and chlorosis symptoms, infected by Polerovirus. RT-PCR technique successfully amplify the target DNA fragment size of 650 bp for Polerovirus and PCR successfully amplified the target DNA fragment size of 700 bp for PepYLCV in accordance with the specific primers were used. Keywords : Chili pepper, mosaic , yellow, chlorosis
Pengaruh Penggunaan Jaring Berwarna Terhadap Kelimpahan Serangga Aphis gossypii pada Tanaman Cabai Rawit (Capsicum frutescens L.) NYOMAN AYU TRISNA ARI UTAMI; I NYOMAN WIJAYA; I KETUT SIADI; I DEWA NYOMAN NYANA; GEDE SUASTIKA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.4, Oktober 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

ABSTRACT Effect of Barier Colored Nets to Abundance of Aphis gossypii on Cayenne Pepper (Capsicum frutescens L.) Cayenne pepper (Capsicum frutescens L.) is a plant that has a high economic value and has a many of uses that as seasoning at household, as an ingredient in various food processing industries and the manufacture of pharmaceuticals. Currently chili productivity in Indonesia is still low therefore needs a national chili can not be optimally. One reason is the insect infestation of  A. gossypii are a double role as pests and disease vectors. The use of red and white netting barier around planting chili is one of the pest control management that is environmental friendly. The purpose of this study was to determine the role of red-and-white nets barier in protect the entry of A. gossypii in chilli planting area. There are three treatments were tested using a red netting, white netting and planting without the use of nets. The results showed that the use of red and white nets can reduce insect populations of A. gossypii on peppers planting area. Average of the highest populations of A. gossypii on treatment without a net, then the white netting treatment and lowest in the red, so that the use of red nets can be used to reduce the population of A. gossypii on chilli crop.   Keywords: Chilli pepper , A. gossypii, colored nets
Pengujian Mutu Benih Beberapa Jenis Tanaman Hortikultura yang Beredar di Bali NI NYOMAN DWI RESPITA NINGSIH; I GUSTI NGURAH RAKA; I KETUT SIADI; GUSTI NGURAH ALIT SUSANTA WIRYA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.7, No.1, Januari 2018
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Seed Quality Testing Several Types of Horticultural Crops Circulating in Bali This study aims to determine the physical quality, viability, and vigor seeds of several types of horticultural crops those circulating in Bali. This research was conducted at Breeding and Seed Technology Laboratory and Plant Disease Laboratory of Udayana University Faculty of Agriculture, which lasted from February to April 2015. The method used is seed testing method with duplo system and using T-Test with three replications. The types of seeds that are tested for their quality consist of seven species: mustard, long bean, eggplant, ercis, cucumber, cayenne, and cabbage. The variables observed were physical quality (moisture content), viability (seed germination), and vigor (synapticity of seed growth). The results showed that the seeds of some horticultural crops circulating in the market in Bali have a pretty good quality. This can be seen from the physical quality test of seeds in the form of moisture content, almost all seeds have a relatively safe water content for storage. The result of seed quality test of percentage of seed germination gain between 90.33% - 97.33% in laboratory test is very good because it is above the standard germination value. The result of the simultaneous vigor test grew up to 59.00% - 69.00% is a good enough vigor value because it is at a level above 50%.
Aplikasi Rizobakteri Pantoea agglomerans untuk Meningkatkan Pertumbuhan dan Hasil Tanaman Jagung (Zea mays, L.) Varietas Hibrida BISI-2 I GUSTI NGURAH RAKA; KHAMDAN KHALIMI; I DEWA NYOMAN NYANA; I KETUT SIADI
Agrotrop : Journal on Agriculture Science Vol 2 No 1 (2012)
Publisher : Fakultas Pertanian Universitas Udayana

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This experiment was conducted in the village of Sibang Gede, District of Abiansemal, BadungRegency on November 2011 until February 2012. The research objective is to enhance the growth andyield of maize hybrid BISI-2 using rhizobacteria Pantoea agglomerans as PGPR in compostformulations. This study used a randomized block design (RBD) single factor with 5 treatments and 5replications. The treatments tested were P. agglomerans BS7a, P. agglomerans BS7b, P. agglomeransBS2a, and P. agglomerans BS5a. All treatments P. agglomerans formulated in the form of compostand given a dose of 50 g/hole. As a control dose of compost 50 g/hole without PGPR. The resultsshowed that the application of rhizobacteria P. agglomerans increased the maximum plant height, netassimilation rate, crop growth rate, chlorophyll content of leaves, fresh weight of roots per plant, ovendried weight of stover per plant, number of seeds per plant, cob length, cob diameter, seed weightmoisture content of 12% per plant and seed yield of 12% water content per hectare.
Efektivitas PGPR Formulasi Kompos Dalam Meningkatkan Ketahanan Tanaman Kedelai terhadap Soybean Stunt Virus I KETUT SIADI; KHAMDAN KHALIMI; I DEWA NYOMAN NYANA; I GUSTI NGURAH RAKA
Agrotrop : Journal on Agriculture Science Vol 7 No 2 (2017)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

Effectiveness of PGPR Compost Formulation in Improving Soybean Plant Resistance to Soybean Stunt Virus. Soybean stunt virus (SSV) is one of important obstacles of soybean production in Indonesia. This virus causes the stunting on soybean plant and may cause the yield losses up to 71%. Eight isolates of plant growth promoting rhizobacteria (PGPR) were isolated and tested for their efficacy to control SSV on soybean. Those isolates are Paj, Pak2, Pa1, Pa3, Pa4, BT, and KT. Application of PGPR was done by soaking the seeds in PGPR solution prior to planting and application of PGPR in compost formulation. Virus concentration and disease incidence were determined using DAS-ELISA. Results of this study showed that application of PGPR in compost formulation suppressed disease incidence caused SSV. Disease incidence on treated plants ranged between 10% to 25%, while all of plant (100%) on un-treated plants were infected. Peroxidase activity on treated plants increased by 80.25% to 97.33% in comparison with un-treated plants. These results suggested that application of PGPR in compost formulation could increase the resistance of soybean against SSV. Hence, PGPR can be considered as one of measures to control SSV on soybean.
Karakterisasi Biokimia Aeromonas hydrophila dan Potensinya Sebagai Pemacu Pertumbuhan Bibit Tanaman Melon Emas I KETUT SIADI; KHAMDAN KHALIMI; DEWA NYOMAN NYANA
Agrotrop : Journal on Agriculture Science Vol 4 No 1 (2014)
Publisher : Fakultas Pertanian Universitas Udayana

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Biochemical Characterization of Aeromonas hydrophila and Its Potential for Growth Stimulatorof Golden Melo Seedlings.The results showed that this research got 20 nitrogen fix bacteria isolate,However, Pg2 isolate could increase the growth of golden melo. The treatment with Pg2 isolatesignificantly increased the golden melo growth. All the plant growth parameters of treated plants weresignificantly higher than those of un-treated control plants (P<0.05). The leaf chlorophyll content, plantgrowth rate, and fresh and dry weights of plant were significantly higher than those of un-treatedcontrol plants. Isolates identified as Enterobacter cloacae PG2 percentage of 98.05% probability.
KARAKTERISASICYMBIDIUM MOSAIC VIRUS (CYMMV) PADA TANAMAN ANGGREK KHAMDAN KHALIMI; KETUT SIADI
Agrotrop : Journal on Agriculture Science Vol 1 No 1 (2011)
Publisher : Fakultas Pertanian Universitas Udayana

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Characterization ofCymbidium mosaic virus (CymMV) on Orchid Plant Orchids are affected by more virus disease problems than most crops, reducing their commercial values considerably. Orchid viruses are widespread in cultivated orchids, withCymbidium mosaic potexvirus (CymMV) being the most prevalent. CymMV high incidence in cultivated orchids has been attributed to the stability and ease of transmission of this virus through cultural practices. CymMV induces floral and foliar necrosis. The virus also reduce plant vigor and lower flower quality, which affect their economic value. The objective of the research is to characterize the virus causing mosaic or chlorotic and necrotic on orchids in West Java. A reverse transcription-polymerase chain reaction (RT- PCR) assays using oligonucleotide primers specific to CymMV were also successfully amplified the regions of the coat protein (CP) gene of the virus. Analysis by using sodium dodecyl sulphate- polyacrylamide gel electrophoresis (SDS-PAGE) revealed that the virus have a major structural protein with an estimated molecular weight of 28 kDa. Aligments of partial nucleotide sequences of the CP gene displayed 86 to 92% homology to CymMV isolates from other countries.
Co-Authors ANAK AGUNG MADE ASTININGSIH GEDE SUASTIKA GEDE SUASTIKA GEDE SUASTIKA Gusti Ngurah Alit Susanta Wirya I Dewa Nyoman Nyana I GEDE PUTU WIRAWAN I Gusti Ngurah Bagus I GUSTI NGURAH BAGUS PRANATA PUTRA I GUSTI NGURAH RAKA I GUSTI NGURAH WISNU PURWADI I KADE DARMAWAN I MADE SUDANA I MADE SUDANA I NYOMAN RAGANATHA I NYOMAN WIJAYA I WAYAN SUKADA I.G.N. Bagus I.M.M. Adnyana I.N. Wijaya K.A. Yuliadhi KADEK DWI UTAMA Khamdan Khalimi NI KADEK AYU WIDAYANTHI NI KADEK DWIK PRADNYAWATI Ni Made Puspawati NI NENGAH DARMIATI NI NYOMAN DWI RESPITA NINGSIH NYOMAN AYU TRISNA ARI UTAMI ROBERTO BOBHOPE ARUAN SHAH KANIGARA ASADDIARI TRISNA AGUNG PHABIOLA WAYAN ADIARTAYASA