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Diana Holidah
Fakultas Farmasi Universitas Jember
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Other

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Pengaruh Vanadil Sulfat Terhadap Ekspresi Protein GLUT-4 pada Mencit yang Menderita Diabetes Mellitus Holidah, Diana; Khotib, Junaidi
JFIOnline | Print ISSN 1412-1107 | e-ISSN 2355-696X Vol 6, No 2 (2012)
Publisher : Indonesian Research Gateway

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Abstract

The Present study was designet to investigate the influence of vanadyl sulphate towards GLUT-4 protein activities in skeletal muscle tissue in streptozotocin induced diabetic mice. Twenty five mice divided into five groups i.e. placebo group, diabetic group, and three treatment groups based on vanadyl's doses (5,30 or 100 mg/kg BW, respectively). Diabetic mice model was induced by twice intraperitoneal administration of streptozotocin. The first dose of streptozotocin is 100 mg/kg that were inject in the first day and then dose 50 mg/kg, in the day 14th. Diabetes occured on 21st day after streptozotocin injection and it was shown by increasing blood glucose level from 151.4 ± 25.1 mg/dL to 237.1 ± 33.0 mg/dL. Administration of vanadyl sulphate at the dose of 5,30 or 100 mg/kg BW was significantly reduces blood glucose concentration (p<0,001). The muscular tissue were harvested on the day 28th and stained using routine histology staining, hematoxylin-eosin for morphological qualitative analysis and immunohistochemical examination to observe the activities of GLUT-4 protein in skeletal muscle. The result showed that vanadyl sulphate restore atrophic condition of muscular cells and inhibit cell necrosis in muscular tissue. On immunohistochemical examination, vanadyl sulphate might increased the GLUT-4 protein activities in skeletal muscle in streptozotocin-induced diabetic mice Keywords : vanadyl sulphate, streptozotocin, diabetes mellitus, GLUT-4 Abstrak Penelitian ini bertujuan untuk mengetahui pengaruh vanadil sulfat terhadap aktivitas protein GLUT-4 pada otot skelet mencit yang menderita diabetes mellitus akibat induksi streptozotocin. Sebanyak 25 ekor mencit dibagi menjadi 5 kelompok, yaitu kelompok kontrol, diabtes dan perlakuan yang diberi vanadil sulfat dengan dosis 5,30 dan 100 mg/kgBB. Keadaan diabetes diinduksi dengan pemberian streptozotocin dosis 100 mg/kgBB pada hari pertama dan 50 mg/kgBB pada hari ke -14. Pada hari ke-21 terjadi peningkatan kadar glukosa dari 151,4 ± 25,1 mg/dL menjadi 237,1 ± 33,0 mg/dL. Pemberian vanadil sulfat selama 7 hari akan menurunkan kadar glukosa darah secara signifikan (p<0,001). Otot skelet diambil pada hari ke-28 dan dipreparasi dengan hematoksilin-eosin untuk diamati histologinya dan secara imunohistokimia untuk pengamatan aktivitas protein GLUT-4. Hasil pengamatan menunjukkan pemberian vanadil sulfat akan memperbaiki keadaan atropi dan menghambat nekrosis pada sel otot skelet. Pengamatan secara imonohistokimia menunjukkan bahwa vanadil sulfat dapat meningkatkan aktivitas GLUT-4. Kata Kunci: vanadil sulfat, streptozotocin, diabetes mellitus, GLUT-4
Pengaruh Vanadil Sulfat Terhadap Ekspresi Protein GLUT-4 pada Mencit yang Menderita Diabetes Mellitus Holidah, Diana; Khotib, Junaidi
Jurnal Farmasi Indonesia Vol 6, No 2 (2012)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (463.862 KB) | DOI: 10.35617/jfi.v6i2.124

Abstract

The Present study was designet to investigate the influence of vanadyl sulphate towards GLUT-4 protein activities in skeletal muscle tissue in streptozotocin induced diabetic mice. Twenty five mice divided into five groups i.e. placebo group, diabetic group, and three treatment groups based on vanadyl's doses (5,30 or 100 mg/kg BW, respectively). Diabetic mice model was induced by twice intraperitoneal administration of streptozotocin. The first dose of streptozotocin is 100 mg/kg that were inject in the first day and then dose 50 mg/kg, in the day 14th. Diabetes occured on 21st day after streptozotocin injection and it was shown by increasing blood glucose level from 151.4 ± 25.1 mg/dL to 237.1 ± 33.0 mg/dL. Administration of vanadyl sulphate at the dose of 5,30 or 100 mg/kg BW was significantly reduces blood glucose concentration (p<0,001). The muscular tissue were harvested on the day 28th and stained using routine histology staining, hematoxylin-eosin for morphological qualitative analysis and immunohistochemical examination to observe the activities of GLUT-4 protein in skeletal muscle. The result showed that vanadyl sulphate restore atrophic condition of muscular cells and inhibit cell necrosis in muscular tissue. On immunohistochemical examination, vanadyl sulphate might increased the GLUT-4 protein activities in skeletal muscle in streptozotocin-induced diabetic mice Keywords : vanadyl sulphate, streptozotocin, diabetes mellitus, GLUT-4 Abstrak Penelitian ini bertujuan untuk mengetahui pengaruh vanadil sulfat terhadap aktivitas protein GLUT-4 pada otot skelet mencit yang menderita diabetes mellitus akibat induksi streptozotocin. Sebanyak 25 ekor mencit dibagi menjadi 5 kelompok, yaitu kelompok kontrol, diabtes dan perlakuan yang diberi vanadil sulfat dengan dosis 5,30 dan 100 mg/kgBB. Keadaan diabetes diinduksi dengan pemberian streptozotocin dosis 100 mg/kgBB pada hari pertama dan 50 mg/kgBB pada hari ke -14. Pada hari ke-21 terjadi peningkatan kadar glukosa dari 151,4 ± 25,1 mg/dL menjadi 237,1 ± 33,0 mg/dL. Pemberian vanadil sulfat selama 7 hari akan menurunkan kadar glukosa darah secara signifikan (p<0,001). Otot skelet diambil pada hari ke-28 dan dipreparasi dengan hematoksilin-eosin untuk diamati histologinya dan secara imunohistokimia untuk pengamatan aktivitas protein GLUT-4. Hasil pengamatan menunjukkan pemberian vanadil sulfat akan memperbaiki keadaan atropi dan menghambat nekrosis pada sel otot skelet. Pengamatan secara imonohistokimia menunjukkan bahwa vanadil sulfat dapat meningkatkan aktivitas GLUT-4. Kata Kunci: vanadil sulfat, streptozotocin, diabetes mellitus, GLUT-4
Studi Aktivitas Antioksidan Senyawa 1-(p-klorobenzoiloksimetil)-5-fluorourasil dengan Metode Molecular Docking dan Metode DPPH (Antioxidant Activity of 1-(p-chlorobenzoyloxymethyl)-5-Fluorouracyl Using Molecular Docking and DPPH Method) Brahmansyah Diar Rosiarto; Ayik Rosita Puspaningtyas; Diana Holidah
Pustaka Kesehatan Vol 2 No 1 (2014)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Cellular damage caused by reactive oxygen species (ROS) free radical and antioxidant activity has an important role as free radical scavenging in body. This study was carried out to evaluate in vitro antioxidant and ligand afinity toward human ROS receptor (PDB code: 3ZBF). In DPPH method, 1-(p-chlorobenzoyloxymethyl)-5-fluorouracil dissolved in etil acetat, while 5-fluorouracil and ascorbic acid as standard dissolved in methanol. The free radical scavenging activity was measured spectrometrically with maximum wavelength at 512 nm. DPPH method show that ascorbic acid have strong antioxidant activity (IC50 = 19.092 ppm), while 1-(p-chlorobenzoyloxymethyl)-5-fluorouracil (IC50 = -2,500,245 ppm) and 5-fluorouracil (IC50 = -4,998 ppm) have not antioxidant activity. Molecular docking (in silico) toward human ROS receptor was indicate 1-(p-chlorobenzoyloxymethyl)-5-fluorouracil (Ki = -6.2 ± 0.04 kkal/mol) has best activity than ascorbic acid (Ki = -4.8 ± 0.19 kkal/mol) and 5-fluorouracil (Ki = -4.6 ± kkal/mol).   Keywords: 1-(p-chlorobenzoyloksimethyl)-5-fluorouracil, 5-Fluorouracyl, antioxidants, DPPH, molecular docking
Uji Aktivitas Antidiabetes Ekstrak Teh Hitam dan Teh Hijau secara In Vitro Menggunakan Metode Inhibisi Enzim α-Glukosidase Diana Holidah; Yasmin Yasmin; Fransiska Maria Christianty
Pustaka Kesehatan Vol 6 No 2 (2018)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v6i2.7573

Abstract

Diabetes mellitus is a group of metabolic diseases characterized by hyperglycemia. Carbohydrates are metabolized into glucose, in gastrointestinal tract than absorbed into the bloodstream and increase blood glucose level. This absorption process is catalyzed by α-glucosidase enzyme. Previous studies showed that tea (Camellia sinensis) extract have antidiabetic activity in mice induced by streptozotocin. The aim of this study was to compared the in vitro activity of tea extract in inhibiting α-glucosidase. Black tea and green trea extracts were tested for α-glucosidase inhibitory activity. Acarbose was used as positive control. IC50 extract was 54.86 µg/mL for black tea and 44.79 µg/mL for green tea. This study showed that inhibitory effect of green tea was higher than black tea. Keywords: black tea, green tea, diabetes mellitus, α-glucosidase
Studi Pengobatan Diabetes Melitus Tipe 2 dengan Komplikasi Hipertensi di Instalasi Rawat Jalan RSU dr. H. Koesnadi Bondowoso Periode Tahun 2014 (Study of Treatment of Type 2 Diabetes Mellitus with Hypertension in Outpatient Departement of dr. H. Koesnadi Sendika Widi Saputri; Antonius Nugraha Widhi Pratama; Diana Holidah
Pustaka Kesehatan Vol 4 No 3 (2016)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Diabetes mellitus (DM) is a chronic disorder characterized by hyperglycemia caused by disruption of glucose metabolism in the body. Non-insulin dependent DM or type 2 DM can progress into macrovascular complication such as hypertension. The aim of this study was to examine treatment profile of patients with type 2 DM and hypertension. This consecutive cross sectional research used the data taken from medical records of ambulatory patients visiting dr. H. Koesnadi Bondowoso during January to December 2014. A number of 205 patients were included in the study consisting of 125 (60.1%) females and 80 (39.0%) males. Most patients (180 patients, 78.2%) were aged 50-69 years. One hundred and eighty-seven patients received antidiabetic and antihypertensive therapy, 4 patients were without antidiabetic therapy and 14 patients without antihypertensive therapy. Inappropiate combination of medications occured among 71 patients. Moreover,12 patients were found under dosed. Keywords: diabetes mellitus, hypertension, antidiabetic, antihypertensive, outpatient
Ekstrak n-Heksana Daun Maja (Aegle marmelos) Menurunkan Kadar LDL Mencit Diabetes yang Diinduksi Aloksan (n-Hexane Extract of Maja Leaves (Aegle marmelos) Decreases LDL Levels on Diabetic Mice Induced by Alloxan) Lesti Eko Pangestuti; Fifteen Aprila Fajrin; Diana Holidah
Pustaka Kesehatan Vol 3 No 1 (2015)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Diabetes mellitus is a metabolic disorder which characterized by hyperglicaemia due to abnormalities of carbohydrate, fat an protein metabolism resulting from defects in insulin secretion, insulin action or both. Metabolic disorders are caused by diabetes induce lipolysis, resulting in changes in the levels of LDL in the blood. This research aims to know the effect of n-hexane extract of maja leaves in LDL levels in diabetic mice. Test animals were induced by alloxan 210 mg/kg BW intraperitoneally. Twenty five mice were divided into 5 groups, control negative group (CMC Na 1%), control positive group (glibenclamide suspension 0.65 mg/kg BW), and the treatment group was given 3 suspension of of n-hexane extract of maja leaves (dose of 200 mg/kg BW, 400 mg/kg BW, and 800 mg/kg BW) for 14 days. The result showed that the decrease of LDL level due to N-hexane extracts of maja leaves dose 200 mg/kg BW, 400 mg/kg BW, and 800 mg/kg BW was -10,54%; 9,07%; 31,69%; respectively. N-hexane extracts of maja leaves decreases LDL levels on diabetic mice induced by alloxan with the best activity indicated by doses 800 mg/kg BW. Keywords: Aegle marmelos, n-hexane, diabetes melitus, LDL, alloxan
Uji Toksisitas Subkronik Jamu Asam Urat pada Hati Mencit Galur Balb-C (Subchronic Toxicity Study of Jamu Asam Urat in Liver of Balb-C Mice) M Nuril Huda; Diana Holidah; Fifteen Aprila Fajrin
Pustaka Kesehatan Vol 5 No 1 (2017)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v5i1.3952

Abstract

Jamu asam urat is one of the most-consumed herbal medicine product to threat gout arthritis. This study aimed to determine of jamu asam urat effect for long-term consumption on mice's liver. Mice were divided into 4 groups of 10 each group. A control group was administered with aquadest and 3 treatment groups were administered with jamu sample per oral in dose of 1,800 (D1); 3,600 (D2); and 7,200 mg/kg BW (D3) in 28 days. The examination included GPT and GOT serum level measurement and liver histopathology. The result showed that GPT serum level was 34.33 ± 2.48 U/L (D1), 41.20 ± 1.94 U/L (D2), and 46.32 ± 2.84 U/L (D3), while GOT serum was 44.60 ± 2.94 U/L (D1), 55.53 ± 3.22 U/L (D2), and 63.31 ± 2.63 U/L (D3), respectively. Parenchymatous degeneration and necrosis in liver were found in histopathology examination. Increase of GPT, GOT level, and parenchymatous degeneration liver changes at D1 and D2 counted as mild degeneration, meanwhile necrosis change at D3 counted as moderate degeneration but it did'nt greatly affect liver function. Thus jamu asam urat is still save to be consumed. Keywords: subchronic toxicity, jamu asam urat, GPT, GOT, liver histopathology
Aktivitas Minyak Jahe Merah (Zingiber Officinale var. rubrum) Terhadap Nyeri Inflamasi pada Mencit Balb-C dengan Induksi CFA (Completed Freund’s Adjuvant) (Activity of Red Ginger Oil (Zingiber officinale var. rubrum) on Inflammatory Pain in Mice Balb-C Wi Fransiska Maria Christianty; Gati Dwi Sulistyaningrum; Fifteen Aprilia Fajrin; Diana Holidah
Pustaka Kesehatan Vol 4 No 3 (2016)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Pain is a multidimentional experiences. Chronic pain is caused by inflammation. Nowdays, the treatment of chronic pain is still a challenge. Plants that known to have analgetic activity is red ginger (Zingiber officinale var. rubrum). This research observed the effect of red ginger oil on inflammatory pain. Inflammatory condition was induced by intraplantar injection of CFA (Completed Freund's Adjuvant). Twenty mice were divided into 4 groups i.e sham, negative control, red ginger oil in two different doses (100 and 200 mg/kg BW). Red ginger oil was administrated per oral once a day for seven consecutive days, a week after CFA injection. Latency time toward thermal stimulus was measured on days 0, 1, 3, 5, 7, 8, 10, 12, and 14 after CFA injection. Paw thickness at the ipsilateral site was also measured on days 0, 1, 3, 5, 7, 8, 10, 12 and 14 after CFA injection. The result showed that the administration of red ginger oil significantly increased latency time toward thermal stimulus compared to negative control. It also significantly reduced paw thickness especially at dose of 200 mg/kg BW. These findings suggested that administration of red ginger oil could treat the inflammatory pain, caused by CFA injection. Keywords: red ginger oil, inflammatory pain, CFA, hyperalgesia
Pengaruh Gel Binahong (Anredera cordifolia (Ten.) Steenis) terhadap Penyembuhan Luka Tikus Diabetes yang Diinduksi Aloksan (The Effect of Binahong (Anredera cordifolia (Ten.)Steenis) Gel on Wound Healing Process of Diabetic Rats Induced by Alloxan) Shinta Cholifatu Rohma; Evi Umayah Ulfa; Diana Holidah
Pustaka Kesehatan Vol 3 No 3 (2015)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Diabetes Mellitus (DM) is a chronic disease that can be indicated by the increase of blood glucose level. Diabetic ulcer is one of complication caused by DM. Binahong (Anredera cordifolia (Ten). Steenis) has a potential effect to heal the wound. The purpose of this study was to examined the effect of binahong gel on the wound healing process of diabetic rats induced by alloxan. The observation was done on the wound's histopathological preparation. The wound were made on diabetic rats, then the binahong gel at a dose of 100 mg and 200 mg were spread on to their wounds. The observation of qualitative descriptive parameters consisted of epithelial tissue, collagen, and new blood vessels formation. The results showed that binahong gel was able to accelerate the wound healing process of diabetic ulcer.   Keywords: Anredera cordifolia, diabetic ulcer, wound healing, alloxan, histopathological profile
IN VITRO ALFA-GLUCOSIDASE INHIBITORY ACTIVITY OF VARIOUS TEA (Camellia sinensis L.) EXTRACTS Fransiska Maria Christianty; Diana Holidah; Yasmin Yasmin
UNEJ e-Proceeding Proceeding of 1st International Conference on Medicine and Health Sciences (ICMHS)
Publisher : UPT Penerbitan Universitas Jember

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Abstract

Diabetes mellitus is a group of metabolicdiseases characterized by hyperglycemia [1]. DM isclassified based on the pathogenic process that leadsto hyperglycemia as opposed to earlier criteria suchas age of onset or type of therapy. There are twobroad categories of DM, designated type 1 and type2 [2]. There were 10 million cases of diabetes inIndonesia in 2015. The number of these cases ispredicted to continue rising [3].Therapeutic approaches for the treatment oftype 2 diabetes, such as diet, sulphonylurea,metformin and insulin therapy, are all successful indecreasing fasting glucose levels. The importance ofpostprandial hyperglycaemia has led to thedevelopment of new agents to control this importantaspect of diabetes.Thus, α-glucosidase enzyme hasbecome one of important target in the treatment oftype-2 diabetes [4,5].α-glucosidase inhibition will slow thedigestion of carbohydrate and absorbtion ofmonosaccahrides in the proximal jejenum [6].Acarbose is an oligosaccharide which reversiblyinhibits intestinal α-glucosidase enzymes responsiblefor digestion of complex carbohydrates anddisaccharides to absorbable monosaccharides [7].The most common adverse effect of acarbose isabdominal discomfort associated with flatulence anddiarrhoea [8]. The undesirable side effects that existunderlie the emergence of research for alternativetherapy of type 2 diabetes, particularly through themechanism of inhibition enzyme α-glucosidase.Tea (Camellia sinensis L.) is annual plant thatwidely spread in Southest Asia, India, South China,Northwest Laos, Thailand and Burma. Tea is one ofthe most popular beverages consumed worldwide[9]. Various studies have reported beneficial effectof tea extract, including for diabetes mellitustherapy. Previous studies showed that tea extracthave antidiabetic activity in mice induced bystreptozotocin [10]. Antidiabetic activity of teaextract have been reported by Yang and Koh. Theresults showed that black tea extract had higheractivity to inhibit α-glucosidase than green teaextract. The present study was designed to comparein vitro α-glucosidase inhibitory activity of varioustea extracts, such as black tea, green tea, oolong tea,and white tea.
Co-Authors Andrean Roni Antonius Nugraha Widhi Pratama Ayik Rosita Puspaningtyas1 Brahmansyah Diar Rosiarto Diana Lady Yunita Handoyo Dwi Aftiningsih Ema Rachmawati Evi Umayah Ulfa Fifteen Aprila Fajrin Fifteen Aprila Fajrin Fifteen Aprilia Fajrin Fifteen Aprilia Fajrin, Fifteen Aprilia Fransiska Maria Christianty Gati Dwi Sulistyaningrum Ika Puspita Dewi Ika Puspita Dewi Ika Puspita Dewi Iskandar P. A. Siregar Jihan Ulya Ulinnuha Junaidi Khotib Lesti Eko Pangestuti M Nuril Huda Mochammad Amrun Hidayat Monica Cinuradha Aura Salsabina Mutmainnatus Solihah Ninda Titis Ainorrochma Sendika Widi Saputri Shinta Cholifatu Rohma Siti Muslichah Sri Nur Atiqah Wilda Zidni Ilma Wiratmo Wiratmo Wiratmo Wiratmo Yasmin Yasmin Yasmin Yasmin