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Pengaruh Bioaktivator Selulolitik untuk Mempercepat Pengomposan Tandan Kosong Kelapa Sawit (Elaeis guineensis Jacq.) Diah Nurul Aini; Hanifa Hanifa; Dessy Sari Mulfa; Tetty Marta Linda
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 6, No 1 (2021): February 2021
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v6i1.3023

Abstract

Oil palm plantations are very extensive in Indonesia which have produced oil palm solid waste in the form of oil palm empty fruit bunches (OPEFB, shells and fibers. Generally, OPEFB is processed into compost but has a constraint that requires a long time of three months. The solution is to provided bioactivators.  This study aims to determine variations in the amount of cellulolytic bioactivators in composting OPEFB that is 0 ml (P1), 50 ml (P2), 75 ml (P3) and 100 ml (P4). Composting is carried out at an incubation laboratory scale for 45 days. The best research results were obtained in P4 treatment with compost quality: N-total of 2.13; C-organic: 35,45 and C/N ratio 16,68 which are in accordance with SNI standards. This cellulotic bioactivator can be developed for the production of TKKS compost on a larger scale.
Potensi Isolat Bakteri Pelarut Fosfat Asal Tanah Gambut Riau dalam Memproduksi Hormon Indole Acetic Acid (IAA) dan Pengaruhnya Terhadap Perkecambahan Benih Cabai Merah (Capsicum annuum L.) Dwi Wahyuni; Tetty Marta Linda; Wahyu Lestari
BIO-SITE |Biologi dan Sains Terapan Vol. 2 No. 2 (2016): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Indole Acetic Acid (IAA) is a group of auxin hormone role in regulating the growth and development. Bacterial isolated from peat soils of Riau known activity in dissolving the phosphate in the Pikovskaya medium and Red-Yellow Podzolic Soil. The purpose of this study was to test isolates from peat soils of Riau in producing IAA hormone and its effects on the germination seeds of red pepper. The results showed that the production ofIAA in Nutrient Broth (NB) not signifficant to all treatments, but tends to be highest on GGO6 that is equal to 9.72 ± 5.91 ppm. The addition of L-tryptophan in the media NB indicated that GGO2 (24.51 ± 5.53 ppm) and GGO6 (19.61 ± 1.80 ppm) significantly to GGO4 (11.33 ± 4.12 ppm). Soaking seeds of red pepper on each bacteria tend to increase the rate of seed germination. Shoot length on GGO5 not different from GGO1, GGO2 and GGO3, but significantly different with the GGO4, GGO6 and control. While GGO1 and GGO3 also significantly different with control. Root length for all treatments significantly different with the control except GGO4.
KEMAMPUAN ISOLAT TUNGGAL DAN KONSORSIUM AKTINOMISETES LOKAL RIAU DALAM MENDEGRADASI HIDROKARBON MINYAK BUMI Novalita Dwi Fanny; Tetty Marta Linda; Atria Martina
BIO-SITE |Biologi dan Sains Terapan Vol. 4 No. 2 (2018): Bio-Site
Publisher : Biology Department, Faculty of Science and Technology, Univeristas Jambi, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (532.94 KB) | DOI: 10.22437/bs.v4i2.5691

Abstract

Pollution of crude oil on environmental can have an effect of nutrient uptake on plants and balance of ecosystems. Biodegradation. is one way to reduce petroleum pollution . The purpose of this research are determine the potential growth of actinomycetes local Riau (L11 and L121) in crude oil hydrocarbons at concentrations of 0%, 2%, 5% and to know their ability to degrade crude oil hydrocarbons at concentrations of 5%. This research observed the growth of each actinomycetes local Riau and the percentage of crude oil degradation by a single isolate and a consortium with gravimetric method. The results showed that the growth of L11 and L121 total population was not significantly different at addition crude oil of 2% and 5% but significantly different wth 0% (without crude oil). Test degradation of crude oil using single isolate gave significantly different results for the consortium isolates. Percentage of degradation crude oil by L11 was 23.5%, L121 was 19.2% and consortium isolate (L11 + L121) was 31.9% with an incubation time of 10 days in a minimum medium salts. This actinomycetes local Riau can be developed as biodegradation agents for crude oil on soil and water.
Aktivitas Ligninolitik Beberapa Jamur Aphyllophorales dan Kemampuannya Mendegradasi Lignin pada Lindi Hitam Atria Martina; Tetty Marta Linda; Delita Zul; Nila Veronika; Ratna Jelita
Al-Kauniyah: Jurnal Biologi Vol 8, No 1 (2015): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v8i1.2702

Abstract

Fourteen local isolate Aphyllophorales fungi were screened their ligninolytic activity. The isolate with highest ligninolytic activity was tested it capability to degrade kraft blackliquor lignin. The biodegradability of black liquor is low because the presence of lignin and lignin derivative in the wastewater. These fungal were screened for ligninolytic activity by decolorization on solid mediacontaining RBBR dye. The ability of the fungal strains to biodegrade kraft black liquor lignin was performed by submerged fermentation condition with agitation and incubation time as treatment. The solid culture result in 3 isolates had ligninolytic activity and Ganoderma sp.BTA1 gave the highest ligninolytic. Agitation and incubation time influenced ligninbiodegradation of blackliquor significantly. Optimum condition for lignin biodegradation was at 200 rpm during 25 days with lignin reduction was 45,786%.
ISOLASI DAN KERAGAMAN BAKTERI UREOLITIK LOKAL RIAU YANG BERPOTENSI SEBAGAI CAMPURAN BETON Mufidah Dwi Suci Ningsih; Tetty Marta Linda; Bernadeta Leni Fibriarti
Al-Kauniyah: Jurnal Biologi Vol 11, No 1 (2018): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (619.771 KB) | DOI: 10.15408/kauniyah.v11i1.5737

Abstract

Abstrak Bakteri ureolitik merupakan mikroorganisme yang memiliki kemampuan untuk menghasilkan urease yang dapat mengendapkan kalsium karbonat (kalsit). Bakteri ini berpotensi sebagai agen bioremediasi logam berat dan sebagai bahan konstruksi beton. Penelitian ini bertujuan untuk mengisolasi, mengkarakterisasi, dan menguji presipitasi kalsit bakteri ureolitik dari tanah lokal Riau. Hasil penelitian memperoleh 30 isolat bakteri yang berhasil diisolasi dari tanah tempat pembuangan akhir, dan setelah dilakukan pewarnaan Gram, 77% isolat merupakan bakteri Gram positif dan hanya 33% merupakan bakteri Gram negatif.  Hasil uji presipitasi menunjukkan bahwa isolat- isolat dengan kode sp. 32, sp. 9, dan sp. 20 mampu membentuk kalsium karbonat berturut-turut sebesar 0,129 g, 0,126 g, dan 0,105 g, setelah diinkubasi selama 7 hari pada medium cair yang diberi penambahan urea dan kalsium. Isolat-isolat bakteri tersebut memiliki hubungan kekerabatan yang dekat, yang ditandai dengan besarnya koefisien kekerabatannya, yaitu lebih dari 70%. Dengan demikian, bakteri-bakteri yang terisolasi dan teruji dalam  membentuk kalsium karbonat asal tanah lokal Riau berasal dari sekelompok bakteri, yang berpotensi untuk dikembangkan sebagai campuran beton.  Abstract Ureolytic bacteria are microorganisms that have the ability to produce urease that precipitates calcium carbonate (calcite). This bacteria has potential as an agent for bioremediation of heavy metal and as a concrete construction material. The aim of this research is concerning about isolation, characterization, and examination on calcite precipitate of the ureolytic bacteria from Riau local soil. The result showed that 30 isolates were isolated from landfill soil, and after Gram staining, 77% of the isolates are Gram-positive and only 33% are Gram-negative. The result of precipitation examination showed that the bacterial isolates sp. 32, sp. 9, and sp. 20 precipitated 0.129 g, 0.126 g and 0.105 g of calcium carbonate, respectively, after incubation for 7 days in broth medium added with urea and calcium. The bacterial isolates have a close relationship, which is characterized by the magnitude of the coefficient of more than 70%. Therefore, the isolated and tested bacteria having the ability to form calcium carbonate from local soil Riau derived from a group of bacteria, which has a potential to be developed as a mixture of concrete. Permalink/DOI: http://dx.doi.org/10.15408/kauniyah.v11i1. 5737  
STUDI PERTUMBUHAN DAN DEGRADASI FENOL OLEH KULTUR TUNGGAL AKTINOMISETES DARI TANAH GAMBUT Tiara Elsita Masni; Tetty Marta Linda; Bernadeta Leni Fibriarti
Al-Kauniyah: Jurnal Biologi Vol 13, No 1 (2020): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2866.46 KB) | DOI: 10.15408/kauniyah.v13i1.12854

Abstract

AbstrakFenol adalah senyawa organik yang bersifat toksik dan larut dalam air, sehingga mudah menimbulkan pencemaran pada perairan dan menurunkan kualitas air. Penelitian ini bertujuan untuk melihat potensi tiga isolat aktinomisetes asal tanah gambut Riau dalam Minimal Salt Medium yang mengandung fenol pada konsentrasi 0 ppm, 200 ppm, 400 ppm, dan 600 ppm serta mengetahui kemampuan aktinomisetes dalam mendegradasi fenol pada konsentrasi 600 ppm menggunakan metode folin ciocalteau. Potensi pertumbuhan isolat L121, L18, L11 menunjukkan total populasi tidak berbeda nyata dengan penambahan 400 ppm dan 600 ppm fenol, tetapi berbeda nyata terhadap 0 ppm dan 200 ppm fenol. Potensi pertumbuhan tertinggi terdapat pada isolat L121 dan terendah pada isolat L11. Kemampuan degradasi  fenol oleh  masing-masing isolat adalah  L121 sebesar 570,80 ppm (95%),  L18 sebesar 218,85 ppm (36%)  dan L11 sebesar  97,21 ppm (16%)  dari konsentrasi fenol awal 600 ppm pada Minimal Salt Medium. Isolat aktinomisetes ini berpotensi dikembangkan untuk penanggulangan pencemaran di lingkungan.Abstract Phenol is an organic compound is toxic and easily soluble in water so easy to cause pollution in a waters such as water quality degradation. The aim of this research is to see the potential of three isolates of actinomycetes from Riau peat soil in Minimal Salt Medium containing phenol concentration 0 ppm, 200 ppm, 400 ppm and 600 ppm and to know the ability of actinomycetes in degradation of phenol at the concentration of 600 ppm using folin ciocalteu. The growth potential of L121, L18, L11 isolates showed the total population was not significantly different with the addition of 400 ppm and 600 ppm of phenol but significantly different from 0 ppm and 200 ppm of phenol. The highest growth potential was found in L121 isolate and lowest in L11 isolate. The degradation ability of phenols by each isolate was L121 570.80 ppm (95%), L18 218.85 ppm (36%) and L11 was able to degrade phenol 97.21 ppm (16%) from the initial phenol concentration of 600 ppm at Minimum Salt Medium.These actinomycetes have the potential to be developed for the overcome of pollution in the environment.
Isolation and Analysis of Bioactive Compounds Endophytic Bacteria of Sea Fern (Acrtostichum aureum L.) from Bengkalis Island, Riau Tetty Marta Linda; Azizul Berlyansah; Bernadeta Leni Fibriarti; Nery Sofiyanti; Silvera Devi
Jurnal Biologi Tropis Vol. 22 No. 1 (2022): January - March
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v22i1.3104

Abstract

Endophytic bacteria have been known to produce high levels of secondary metabolites. The sterile leaves of the sea fern (Acrostichum aureum L.) contain many phytochemical compounds. This sea fern is often found in peat swamp areas. The aim of this study was to isolate, partial characterization, and analysis of bioactive compounds from endophytic bacteria of sea ferns to determine their bioactive compounds. Endophytic bacteria were isolated by a direct technique using nutrient agar (NA) media. In this study, the endophytic bacteria were characterized by macroscopic, microscopic, and Gram. Several tests were further performed, namely phytochemical test and Fourier Transform Infra Red (FT IR) FTIR tests from a crude extract of secondary metabolites of bacteria. The results of the isolation obtained six isolates of endophytic bacteria consisting of all isolates were Gram-negative. The phytochemical test results for the six isolates were positive for alkaloids and saponins but no steroids. Based on the results of phytochemical tests and FT IR, it is suspected that the isolate DSB 1.6 has similarities with the alkaloid functional group. This endophytic bacterial isolate can be developed as a candidate for produced of pharmacological compounds.
Pengolahan Sagu menjadi mie prebiotik sebagai makanan fungsional dan upaya peningkatan ketahanan pangan di Desa Alai Selatan, Kecamatan Tebing Tinggi Barat, Kepulauan Meranti Atria Martina; Wahyu Lestari; Tetty Marta Linda; Saberina Hasibuan; Imelda Wardani
Unri Conference Series: Community Engagement Vol 2 (2020): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.2.112-116

Abstract

Noodles are a processed food product favored by most Indonesians, generally made from wheat which must be imported. Kepulauan Meranti Regency is the largest producer of sago in Indonesia. One of the products made from sago that is widely produced in Meranti is sa.go noodle. Making noodles with local raw materials such as sago and sweet potato is an effort to improve food security. Traditional sago noodle has high carbohydrate and fiber content but still has disadvantages such as not having inulin content as a prebiotic, less antioxidant and a less attractive color, while purple sweet potato contains inulin as prebiotic components, high antioxidants, vitamins and minerals.The utilization of prebiotics insago noodle has multiple advantages since they improved probiotic growth in the body, sensory features and provide a more well-balanced nutritional composition as functional food. This community service program aims to training on the process of making prebiotic sago noodles to the community in Alai Selatan Village.This activities are carried out by course and practice methods.The results showed that the participants were interested in making prebiotic sago noodles because they had a more attractive features, more nutritious and had a delicious taste. Prebiotic sago noodles can be used as an effort to biodiversify sago noodle products to support food security and improve the community's economy.
Pemberdayaan masyarakat di Desa Balai Makam dalam pemanfaatan bonggol pisang sebagai rintisan Usaha Mikro Kecil Menengah (UMKM) Tetty Marta Linda; Sezhianindi Firiola; Rahmah Agnissah; Binar Robinson M; Muhammad Rinaldi; Sri Ramadhani
Unri Conference Series: Community Engagement Vol 2 (2020): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.2.344-351

Abstract

Banana hump is one part of the banana plant that is still very rarely used. The Banana hump can be an item with a selling value because it contains high carbohydrates, fiber, and calcium to be an alternative food ingredient. The banana hump can be turned into nutrient-rich flour. Community empowerment to process banana hump into something useful and can be a pilot business for residents is one of the keys to this success of this activity. This service will provide benefits to various parties, including for community service participants, for the counseling implementation team, for universities, and for the government. The purpose of this activity is to introduce and train the skills of making flour made from banana humps, which we named "Tebosang" for the community of Balai Makam Village, Bathin Solapan District, Bengkalis Regency. The methods used include site surveys, interactive training preparation, mentoring, monitoring, and evaluation. Making flour from banana hump started with a waste of old banana hump, cleaned the surface, then sliced thin approximately 0.5 cm. The hump then washed and then soaked with whiting for about one hour, then rinsed and dried. The dried hump slices are then blended and then sifted so that they become flour. The community's enthusiasm was relatively high at the time of the training, shown by the community's involvement at the demonstration. The monitoring and evaluation results show that the community already understands how to make flour from the banana hump. The result also showed that the village community is already producing "tebosang" flour, which has commercial potential, a start-up for micro, small and medium enterprises in each community.
Identifikasi Molekuler Bakteri Endofit Tanaman Karet (Hevea Brasiliensis) dan Aktivitasnya dalam Memproduksi Inhibitor Alfa Amilase Vanisa Sri Elvani; Syauqi Susana Rahmani; Safrida Dwiningsih; Tetty Marta Linda; Windi Dona Fitri Fitri
Metamorfosa: Journal of Biological Sciences Vol 10 No 1 (2023)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2023.v10.i01.p02

Abstract

Endophytic bacteria are sized microorganisms found in plant tissues that are known to produce secondary metabolites, one of which is the ?-amylase inhibitor. Plant potential is closely related to endophytic microorganisms present in plant tissues. The study aimed to determine the potential of endophytic bacteria in rubber plants to produce ?-amylase inhibitors and to identify molecular using the 16S rRNA gene sequence. The isolates used were endophytic bacteria isolates L1M1.A2, L1M1.B4, and L2M1.A27. The activity of ?-amylase inhibitor was tested by in vitro and spectrophotometric measurements at a wavelength of 540 nm. Endophytic isolates with the highest inhibitory values ??were identified using the 16S rRNA gene sequence with primers 8F and 1492R. The results showed that the L1M1.A2 isolate produced 29.44% ?-amylase inhibitor and was identified as Bacillus proteolyticus with 99.86% homology. The potential of the L1M1.A2 isolate could be developed as a candidate for an antidiabetic drug.