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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) ANNALES BOGORIENSES OLDI (Oseanologi dan Limnologi di Indonesia) Marine Research in Indonesia Pharmaceutical Sciences and Research (PSR) Makara Journal of Science
PUSPITA LISDIYANTI
Pusat Penelitian Bioteknologi, Lembaga Ilmu Pengetahuan Indonesia (LIPI)
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Environmental Science Immunology & microbiology Medicine & Pharmacology Veterinary

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Editorial Boards AB Vol 14 No 1 (2010) Lisdiyanti, Puspita
ANNALES BOGORIENSES Vol 14, No 1 (2010): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2543.383 KB) | DOI: 10.1234/140

Abstract

Enhancement of β-Glucosidase Activity in Penicillium sp. by Random Mutation with Ultraviolet and Ethyl Methyl Sulfonate Syafriana, Vilya; Nuswantara, Sukma; Mangunwardoyo, Wibowo; Lisdiyanti, Puspita
ANNALES BOGORIENSES Vol 18, No 2 (2014): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (450.422 KB) | DOI: 10.1234/98

Abstract

The genus Penicillium has a potential ability to produce β-glucosidase. The aim of the study was to improve the β-glucosidase activity of Penicillium sp. ID10-T065 with physical (Ultraviolet = UV), chemical (Ethyl Methyl Sulfonate = EMS), and combined mutation (UV-EMS). The spores of Penicillium sp. ID10-T065 were exposed into UV irradiation for 3 minutes with dose of 0.1 J/cm2 and 13 cm of distances. Chemical mutation was done by treated spores into 3% of EMS solution for an hour. Combined mutation of UV and EMS were also performed by UV for 3 minutes (0.1 J/cm2, 15 cm) and continued with soaking into 2-3% of EMS solution. The developed mutants were screened, selected and assayed. Comparison of enzyme activities with the wild- type (1.78 U/ml), mutant UV13 (5.53 U/ml) showed a 3.1 fold increase; mutant EM31 (4.26 U/ml) showed a 2.4 fold increase. Meanwhile, mutant UM23 obtained from the multiple exposures showed a decreased activity (1.75 U/ml). Mutant UV13 showed the best enzyme activity to be considered as a potential strain for β-glucosidase producer. This result needs to be further elaborated especially on its genetic stability studies in order for the ascertained as a stable mutant.
Editorial Boards AB Vol 17 No 1 (2013) Lisdiyanti, Puspita
ANNALES BOGORIENSES Vol 17, No 1 (2013): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/172

Abstract

Guide for Authors AB Vol 16 No 1 (2012) Lisdiyanti, Puspita
ANNALES BOGORIENSES Vol 16, No 1 (2012): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/163

Abstract

Back Cover AB Vol 12 No 1 (2008) Lisdiyanti, Puspita
ANNALES BOGORIENSES Vol 12, No 1 (2008): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/129

Abstract

Editors Preface AB Vol 18 No 2 (2014) Lisdiyanti, Puspita
ANNALES BOGORIENSES Vol 18, No 2 (2014): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (132.34 KB) | DOI: 10.1234/186

Abstract

Front Cover AB Vol 15 No 2 (2011) Lisdiyanti, Puspita
ANNALES BOGORIENSES Vol 15, No 2 (2011): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (130.993 KB) | DOI: 10.1234/154

Abstract

Editorial Boards AB Vol 10 No 1 (2005) Lisdiyanti, Puspita
ANNALES BOGORIENSES Vol 10, No 1 (2005): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (316.47 KB) | DOI: 10.1234/119

Abstract

Editorial Boards AB Vol 17 No 2 (2013) Lisdiyanti, Puspita
ANNALES BOGORIENSES Vol 17, No 2 (2013): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/177

Abstract

Back Cover AB Vol 14 No 2 (2010) Lisdiyanti, Puspita
ANNALES BOGORIENSES Vol 14, No 2 (2010): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (116.262 KB) | DOI: 10.1234/145

Abstract

Co-Authors - Yopi Abd. Rasyid Syamsuri Andi Utama ANDI UTAMA ANDO KATSUHIKO Apriliana, Pamella Ariani Hatmanti Ashadi Sasongko Caniago, Asnany Endang Saepudin, Endang Endang Sukara, Endang GINA KARTINA Herman Suryadi Herman Suryadi JAKA WIDADA Kusharyoto, Wien Kusumawati, Dinihari Indah Kusumawati, Dinihari Indah Lina Elfita Lina Elfita Meliah, Siti Meliah, Siti Mia Kusmiati MISA OTOGURO Nanik Rahmani NANIK RAHMANI Putri, Ade Lia Putri, Ade Lia ROHMATUSSOLIHAT ROHMATUSSOLIHAT RONI RIDWAN Shanti Ratnakomala SHANTI RATNAKOMALA Siti Meliah Subagus Wahyuono Sukma Nuswantara Susanti, Agustina Eko Tisnadjaja, Djadjat TOMOHIKO TAMURA Tutik Murniasih WIBOWO MANGUNWARDOYO Yantyati Widyastuti Yopi Yopi YOPI YOPI Yopi, YULIN LESTARI