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All Journal SAINS MEDIKA : JURNAL KEDOKTERAN DAN KESEHATAN Jurnal Kedokteran Diponegoro Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Journal of Biomedicine and Translational Research Bioscientia Medicina : Journal of Biomedicine and Translational Research Medica Hospitalia Bioscientia Medicina : Journal of Biomedicine and Translational Research Life Science
Nani Maharani
Department Of Pharmacology And Therapy, Faculty Of Medicine, Diponegoro University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

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PERBEDAAN MEMORI JANGKA PENDEK SEBELUM DAN SESUDAH MENDENGARKAN MUSIK SAAT LARI PADA DEWASA MUDA Ardyarini, Hikmatunnisa Tri; Muniroh, Muflihatul; Maharani, Nani
DIPONEGORO MEDICAL JOURNAL (JURNAL KEDOKTERAN DIPONEGORO) Vol 7, No 2 (2018): JURNAL KEDOKTERAN DIPONEGORO
Publisher : DIPONEGORO MEDICAL JOURNAL (JURNAL KEDOKTERAN DIPONEGORO)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (418.651 KB)

Abstract

Latar Belakang: Mendengarkan musik saat berolahraga diketahui dapat menimbulkan efek ergogenik dan mempengaruhi sistem kardiovaskuler. Namun, pengaruh kombinasi keduanya terhadap memori, sebagai salah satu fungsi kognitif yang penting pada proses pembelajaran usia dewasa muda, masih belum diteliti lebih lanjut.Tujuan: Mengetahui perbedaan memori jangka pendek sebelum dan sesudah mendengarkan musik saat lari pada kelompok dewasa muda.Metode: Penelitian eksperimental dengan metode pre- dan post-test unequivalent group di Stadion Universitas Diponegoro. Subjek adalah kelompok usia dewasa muda (17-22 tahun) yang pada periode penelitian tercatat sebagai mahasiswa Fakultas Kedokteran Universitas Diponegoro (n=40) dan dipilih secara purposive sampling, kemudian dibagi menjadi 2 kelompok; kelompok lari selama 30 menit dengan mendengarkan musik (n=20) dan kelompok lari selama 30 menit tanpa medengarkan musik sebagai kontrol (n=20). Memori jangka pendek diukur dengan Scenery Picture Memory Test dan analisis data dilakukan dengan uji t-berpasangan, t-tidak berpasangan, Wilcoxon dan Mann-Whitney.Hasil: Terdapat perbedaan memori jangka pendek yang bermakna sebelum dan sesudah mendengarkan musik saat lari (p=0,00). Memori jangka pendek setelah lari dengan mendengarkan musik meningkat secara signifikan (p<0,05) dibandingkan dengan kontrol, dengan rerata peningkatan sebesar 5,0 ± 2,66 pada kelompok lari dengan mendengarkan musik dan 3,05 ± 1,76 pada kelompok kontrol.Kesimpulan: Mendengarkan musik saat lari dapat meningkatkan fungsi memori jangka pendek lebih tinggi dibandingkan dengan lari tanpa mendengarkan musik pada usia dewasa muda.
Perbedaan Kadar IgA dan IL6 Pada Penumpang “Biosmart and Safety Bus” pada Memakai Masker Herbal dan Masker Medis Rahajeng, Herera; Prasetyo, Awal; Susilaningsih, Neni; Sadhana, Udadi; Maharani, Nani; Asri, Hairul
Life Science Vol 10 No 1 (2021): April 2021
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v10i1.47177

Abstract

Viral infections that attack the respiratory tract are currently a major problem globally. The spread of viral infection can be one of the risks by passengers of the bus. The government's efforts being developed are the application of biosmart and safety buses designed to prevent the virus with stake holders. One of the health protocols is the use of masks. The purpose of this study was to see the differences in IgA and IL6 levels before and after the use of medical masks and herbal masks. Nasal wash samples were taken and analyzed by ELISA. The results obtained were the level of IgA for the control group before wearing medical masks was 23.46834 pg/ml and after wearing 29.74473 pg/ml. While the IL6 levels before were 1.10544 ng / ml and after -2.04119 ng/ml. The results of the IgA in the experimental group before using the herbal mask 24.00840 pg/ml and after using was 29.74473 pg/ml. Meanwhile, the level of IL6 before was 0.91682 ng/ml and after 0.500157 ng/ml. Based on the results, there was an increase in IgA levels after the use of medical masks and herbal masks while IL6 levels decreased after the use of medical and herbal masks. Keywords: Herbal mask; IgA; IL6, Masker herbal;IgA;IL6 Infeksi virus yang menyerang saluran pernapasan saat ini menjadi masalah utama secara global. Penyebaran agen infeksi virus dapat menjadi salah satu risiko yang dihadapi penumpang moda transportasi Bus. Upaya Pemerintah seperti yang saat ini dikembangkan adalah pengaplikasian biosmart and safety bus yang dirancang guna menghambat persebaran virus dengan stake holder yang tepat. Salah satu penerapan protokol kesehatan adalah penggunaan masker. Tujuan dari penelitian ini adalah melihat perbedaan kadar IgA dan IL6 sebelum dan sesudah pemakaian masker medis dan masker herbal pada penumpang biosmart and safety bus. Pengambilan sampel nasal wash dilakukan dan dianalisis dengan ELISA. Penelitian ini merupakan quasi experimental dengan prepost test randomized control design. Hasil yang didapat adalah kadar IgA untuk kelompok kontrol sebelum pemakaian masker medis 23,46834 pg/ml dan setelah memakai masker medis 29,74473 pg/ml. Sementara kadar IL6 sebelum adalah 1,10544 ng/ml dan setelah -2,04119 ng/ml. Hasil IgA kelompok eksperimen sebelum pemakaian masker herbal 24,00840 pg/ml dan setelah memakai masker herbal 29,74473 pg/ml. Sementara kadar IL6 sebelum adalah 0,91682 ng/ml dan setelah 0,500157 ng/ml. Berdasarkan hasil penelitian terdapat peningkatan kadar IgA setelah pemakaian masker medis dan masker herbal sementara kadar IL6 mengalami penurunan setelah pemakaian masker medis dan masker herbal. Keywords: Herbal mask; IgA; IL6, Masker herbal;IgA;IL6
PENGARUH EKSTRAK EDAMAME KAYA GENISTEIN TERHADAP KADAR LOW DENSITY LIPOPROTEIN, HIGH SENSITIVITY-C REACTIVE PROTEIN, SERTA RASIO NEUTROFIL LIMFOSIT DAN MONOSIT LIMFOSIT: Studi Experimental pada Tikus Jantan yang Diinduksi Aterosklerosis Reza Dian Pratama; Nani Maharani; Udin Bahrudin
Medica Hospitalia : Journal of Clinical Medicine Vol. 8 No. 1 (2021): Med Hosp
Publisher : RSUP Dr. Kariadi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (323.892 KB) | DOI: 10.36408/mhjcm.v8i1.450

Abstract

ABSTRACT Background: Atherosclerosis is related to hypercholesterolaemia and inflammation. Edamame is one of the soybeans with high levels of genistein which protects the formation of atherosclerosis. Purpose: This research aimed to evaluate the effects of genistein-rich edamame on LDL, hs-CRP, Neutrophil-Lymphocyte Ratio (NLR) and Monocyte-Lymphocyte Ratio (MLR) on atherosclerosis-induced mice. Method: This research is a true experimental study with posttest only-controlled group design. Thirty male rats were divided randomly into five groups: negative control group without any intervention, positive control group with atherosclerosis induction, first intervention group with edamame extract supplementation, second intervention group with genistein-rich edamame extract supplementation, and third intervention group treated with atorvastatin. After 28 days, serum LDL, hs-CRP, LDL and MLR were measured. Results: There were significant differences (p<0.001) of hs-CRP values between the second intervention group compared to the negative control, positive control and first intervention group. Edamame extract lowered LDL cholesterol levels in the second intervention group compared to positive control, but the difference were not statistically significant. There were significant differences (p<0.05) of MLR values between the second intervention group and the negative control and also the first intervention group, and significant differences between the second intervention group and the negative control and also the first intervention group. There was no significant difference on NLR values between all groups. Conclusion: The administration of genistein rich edamame may reduces the inflammation in the blood vessels better than edamame extract represented by lower hs-CRP and MLR values compared to the edamame extract. Keywords: Genistein rich-edamame, LDL, hsCRP, neutrophil-lymphocyte ratio, monocyte-lymphocyte ratio
Utilization of banana (Musa paradisiaca L.) peel as pectin source as antidiarrheal on castor oil-induced diarrheal Wistar rats model Martin Adhitya Subagio; Cecilia Noviyanti Salim; Qorina Putri Srisantoso; Yora Nindita; Astika Widy Utomo; Nani Maharani
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 52, No 2 (2020)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (178.066 KB) | DOI: 10.19106/JMedSci005202202001

Abstract

Banana (Musa paradisiaca L.) peel, locally name pisang raja, contains a high concentration of pectin which is used as antidiarrheal to absorb irritants and increase stool consistency. However, it’s use to increase the stool consistency has not been studied, yet. The study aimed to investigate the effect banana peel extract (BPE) administration on stool consistency of diarrheal rat model. It was an experimental study with a post-test only control group design. Thirty male Wistar rats were induced diarrhea with castor oil and then randomized into five groups. Group I as positive control group was given attapulgite at dose of 124 mg/kg BW. Group II as negative control was given 0.5 mL of 1% tween 80. Group III, IV and V as treatment group were give BPE at different doses of 100, 200 and 400 mg/kg BW, respectively.T he stool consistency was measured every hour for 4 h by weighing fresh and dry stool weight and then the water content was calculated. Data analysis was conducted using one-way ANOVA and LSD post hoc test. Significantly different in stool consistency between Group III, IV, and V compared to Group II (p<0.05) and between groups Group III and IV compare to Group I (p<0.05) were observed. However, there was no significantly different between group V compared to Group I (p=0.149).In conclusion, BPE can increase the stool consistency of castor oil-induced diarrhea rats. The BPE at the dose of 400 mg/kg BW has similar effect to attapulgite.
Antidiarrheal Effect of Lime Peel Extract on Bisacodyl-Induced Mice Abraham Talent Bawadi Sibarani; Hesti Triwahyu Hutami; Nani Maharani; Yora Nindita
JURNAL KEDOKTERAN DIPONEGORO (DIPONEGORO MEDICAL JOURNAL) Vol 10, No 6 (2021): JURNAL KEDOKTERAN DIPONEGORO (DIPONEGORO MEDICAL JOURNAL)
Publisher : Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/dmj.v10i6.32144

Abstract

Background: Diarrhea is a condition of increased fecal water content of more than 200 grams or 200 ml/24 hours with a liquid or semi-liquid consistency. Complications of diarrhea, dehydration and malnutrition are still world health problems. Lime peel extract contains tannins which have astringent properties. Tannins are indicated as an alternative anti-diarrheal agent by tightening and forming a protective layer on the intestinal mucosa thereby reducing osmolarity.Aim: To examine the consistency, fecal water content, and frequency of defecation of bisacodyl-induced diarrhea in mice that are given lime peel extract.Methods: This research was an experimental with post test only group design. Thirty-six male mice were included and then divided into 5 groups with bisacodyl induction and 1 normal control group (KM) without bisacodyl induction. The negative control group (KN) was given 0.5 ml Tween 80 1%, the positive control group (KP) was given attalpugite 0.4 mg/40gW, treatment group 1 (X1), treatment group 2 (X2), treatment group 3 (X3 ) were given lime peel extract at graded doses of 125, 250, and 500 mg/KgW. Consistency, fecal water content, and frequency of defecation were observed every 30 minutes for 4 hours by observing the consistency of feces, weighing wet and dry feces to measure the water content, and measuring the frequency of defecation in each group. Data were analyzed statitiscally using Kruskal Wallis test with Post Hoc Mann Whitney test.Results: There were significant differences between the comparison of defecation frequency in the X3 group and the KM group, for the fecal water content comparison between the X3 group and the KN group, and no significant differences in the following group comparisons.Conclusion: Lime peel extract cannot reduce the frequency of defecation, fecal water content, and increase the consistency of feces significantly.Keywords: Diarrhea, lime peel extract, tannins, astringent, consistency, fecal water content, frequency of defecation.
THE EFFECT OF CORIANDER LEAF EXTRACT TOWARDS KIDNEY HISTOPATHOLOGICAL FEATURES ON WISTAR RAT INDUCED BY ORALLY ADMINISTERED MERCURY Manda Petrina; Ika Pawitra Miranti; Nani Maharani; Intan Rahmania Eka Dini
JURNAL KEDOKTERAN DIPONEGORO (DIPONEGORO MEDICAL JOURNAL) Vol 10, No 4 (2021): JURNAL KEDOKTERAN DIPONEGORO (DIPONEGORO MEDICAL JOURNAL)
Publisher : Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/dmj.v10i4.30095

Abstract

Background: Mercury’s a toxic heavy metal that damages the kidney through generation of Reactive Oxygen Species (ROS). Previous study has established that coriander leaves contained high level of antioxidants. However, there hasn’t been any experiment that examined renoprotective effect of coriander leaf extract toward the kidney of Wistar rat induced with orally administered mercury.Objective: To examine the nephroprotective activity of coriander leaf extract towards Wistar rat’s proximal tubules induced with orally administered mercuryMethods: : Experimental study with post test only control group design using 20 male Wistar rats divided randomly into 4 groups as samples. Group K0 without treatment, Group K(-) was administered 10 mg/ kgBW mercury chloride orally, Group P1 was administered 10 mg/kgBW mercury chloride and 100 mg/kgBW coriander leaf extract orally, and Group P2 was administered 10 mg/kgBW  mercury chloride and 200 mg/kgBW coriander leaf extract orally. The study was carried for 14 days after which the kidneys were examined microscopically.Results: The mean values for damaged proximal tubules were as follows: group K0 2,44±1,19; group K(-) 4,76±3,77; group P1 4,52±2,18; group P2 2,60±1,38. Mann-Whitney test showed significant differences between group K(-)>K0 (p=0,009); group P1>K0 (p=0,001); group P2<K(-) (p=0,015); group P1>P2 (p=0,001). Insignificant differences were found between group P2> K0 (p=0,936) and group P1<K(-) (p=0,579).Conclusion: Coriander leaf extract could reduce the number of damaged proximal tubules from mercury ingestion, with dose of 200 mg/kgBW showing better result than 100 mg/kgBW.Keywords: antioxidants, coriander leaf, kidney, mercury
Hyperuricemia as a Risk Factor for Cardiovascular Diseases Ichiro Hisatome; Peili Li; Fikri Taufiq; Nani Maharani; Masanari Kuwabara; Haruaki Ninomiya; Udin Bahrudin
Journal of Biomedicine and Translational Research Vol 6, No 3 (2020): December2020
Publisher : Faculty of Medicine, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v6i3.9383

Abstract

Serum uric acid level above 7 mg/dl is defined as hyperuricemia, which gives rise to the monosodium urate (MSU), causing gout and urolithiasis. Hyperuricemia is an independent risk factor as well as a marker for hypertension, heart failure, atherosclerosis, atrial fibrillation, and chronic kidney disease. MSU crystals, soluble uric acid (UA), or oxidative stress derived from xanthine oxidoreductase (XOR) might be plausible explanations for the association of cardio-renovascular diseases with hyperuricemia. In macrophages, MSU activates the Nod-like receptor family, pyrin　domain containing 3(NLRP3) inflammasome, and proteolytic processing mediated by caspase-1 with enhanced interleukin (IL)-1β and IL-18 secretion. Soluble UA accumulates intracellularly through UA transporters (UAT) in vascular and atrial myocytes, causing endothelial dysfunction ad atrial electrical remodeling. XOR generates reactive oxygen species (ROS) that lead to cardiovascular diseases. Since it remains unclear whether asymptomatic hyperuricemia could be a risk factor for cardiovascular and kidney diseases, European and American guidelines do not recommend pharmacological treatment for asymptomatic patients with cardio-renovascular diseases. The Japanese guideline, on the contrary, recommends pharmacological treatment for hyperuricemia with CKD to protect renal function, and it attaches importance of the cardio-renal interaction for the treatment of asymptomatic hyperuricemia patients with hypertension and heart failure.
Adiponutrin and Adiponectin Gene Variants in Indonesian Patients with Non-Alcoholic Fatty Liver Disease: a Preliminary Study Rayvita AN Meagratia; Ferdy Kurniawan Cayami; Udin Bahrudin; Wiwik Lestari; Nani Maharani; Sultana MH Faradz; Hery Djagat Purnomo
Journal of Biomedicine and Translational Research Vol 7, No 2 (2021): August 2021
Publisher : Faculty of Medicine, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v7i2.11777

Abstract

BackgroundVariants of adiponutrin (PNPLA3) and adiponectin (ADIPOQ) genes were considered to be associated with non-alcoholic fatty liver disease (NAFLD). Although the prevalence of NAFLD is increasing, there are limited numbers of studies about the association in Indonesian population.ObjectiveTo confirm whether specific variants of adiponutrin (PNPLA3) and adiponectin (ADIPOQ) genes are associated with NAFLD in Indonesian patients.MethodsData and DNA of 152 participants were obtained from a previous study in Dr. Kariadi Hospital, Semarang, Indonesia. PCR-RFLP analysis was performed for detection of PNPLA3 rs738409 and ADIPOQ rs2241767 variants. The diagnosis and severity of NAFLD were assessed according to NAFLD activity score (NAS) based on histopathology assessment of liverbiopsy.ResultsAllele G of PNPLA3rs738409 was associated with NAFLD in both bivariate (p=0.009, OR 2.52, CI 95% 1.25–5.07) and multivariate (p=0.008, OR 2.62, CI 95% 1.29%–5.32%) analysis, while ADIPOQ rs2241767 had no significant association. In NAFLD participants, both genotypes showed allele G was higher in the group of possible non-alcoholic steatohepatitis (NASH) – NASH (NAS >2) than in the simple steatosis group (NAS <2) i.e. 40.0% vs. 3.75% for the rs2241767 variant and 23.75% vs. 1.25% for the rs738409 variant, without significant association.ConclusionVariant PNPLA3 rs738409 was associated with NAFLD incidence in studied population. Among NAFLD participants, the frequency of both variants were found higher in the possible NASH – NASH group, yet needs to be confirmed with more participants and a multicenter study.Data and DNA of 152 participants were obtained from a previous study in Dr. Kariadi Hospital, Semarang, Indonesia. PCR-RFLP analysis was performed for detection of PNPLA3 rs738409 and ADIPOQ rs2241767 variants. The diagnosis and severity of NAFLD were assessed according to NAFLD activity score (NAS) based on histopathology assessment of liverbiopsy.
Diagnostic value of SARS-CoV-2 RDT-Ab with RT-PCR: Secondary data at Diponegoro National Hospital Muhammad Thifan Satyagraha; Nani Maharani; Rebriarina Hapsari; Meita Hendrianingtyas
Journal of Biomedicine and Translational Research Vol 8, No 1 (2022): April 2022
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v1i1.13759

Abstract

Background: The SARS-CoV-2 rapid diagnostic test antibody (RDT-Ab) was most often used as an early detection tool for COVID-19 at the beginning of pandemic. Whereas the antibody response was formed in the second week after the onset of symptoms.Objective: To evaluate the diagnostic value of the SARS-CoV-2 RDT-Ab, including sensitivity (Se), specificity (Sp), positive predictive value (PPV), negative predictive value (NPV), positive likelihood ratio (PLR), and negative likelihood ratio (NLR), in patients at Diponegoro National Hospital, Semarang, Indonesia.Methods: Data subjects have been selected retrospectively using purposive sampling based on inclusion criteria (patients who had shortness of breath, pneumonia, suspected, possible, or confirmed COVID-19, and data on the results of the SARS-CoV-2 RDT-Ab IgM and/or IgG (Leccurate® SARS-CoV-2 Antibody Rapid Test Kit) with a valid RT-PCR as gold standard) and exclusion criteria (patients who only had one of either SARS-CoV-2 RDT-Ab or RT-PCR). Researchers analyzed the diagnostic value of SARS-CoV-2 RDT-Ab with RT-PCR which gave the possibility of true-positive, false-positive, true-negative, and false-negative results arranged in a 2x2 table. According to WHO, the diagnostic value is said to be good at least having a sensitivity value of 80% and specificity of 97%.Results: The diagnostic value of SARS-CoV-2 RDT-Ab with RT-PCR, which was evaluated from 1142 patients retrospectively, included IgM (Se 65.25%, Sp 89.51%, PPV 46.70%, NPV 94.81%, PLR 6.22, NLR 0.39), IgG (Se 58.16%, Sp 93.01%, PPV 53.95%, NPV 94.04%, PLR 8.32, NLR 0.45), IgM and IgG (Se 53.90%, Sp 94.21%, PPV 56.72%, NPV 93.55%, PLR 9.30, NLR 0.49), IgM and/or IgG (Se 69.50%, Sp 88.31%, PPV 45.58%, NPV 95.36%, PLR 5.95, NLR 0.35).Conclusion: SARS-CoV-2 RDT-Ab (Leccurate® SARS-CoV-2 Antibody Rapid Test Kit) is not ideal to be used as a rapid diagnostic test for COVID-19.Keywords: COVID-19, Rapid diagnostic test, RT-PCR, SARS-CoV-2 antibody
Pengaruh Kombinasi Suplementasi Omega 3 dengan Kalori Restriksi Terhadap Penyembuhan Luka Bakar Pada Tikus Sprague Dawley Kajian Pada: Tumor Nekrosis Faktor - Alfa dan Perbaikan Luas Luka Bakar Erwin Wibowo; Edi Dharmana; Nani Maharani; Selamat Budijitno
Medica Hospitalia : Journal of Clinical Medicine Vol. 7 No. 1 (2020): Med Hosp
Publisher : RSUP Dr. Kariadi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (291.137 KB) | DOI: 10.36408/mhjcm.v7i1.432

Abstract

Latar Belakang: Luka bakar merupakan masalah klinis dengan komplikasi yang sering menyebabkan morbiditas dan mortalitas. Omega 3 memiliki efek anti inflamasi dan restriksi kalori memiliki efek anti oksidan yang diduga mampu mempercepat proses penyembuhan pada luka. Penelitian bertujuan untuk mengetahui apakah kombinasi suplementasi Omega 3 dan restriksi kalori dapat membantu dalam proses penyembuhan luka bakar yang dapat ditinjau dari kadar TNF Alfa dan perbaikan luas luka bakar. Metode: Penelitian eksperimental dengan desain “Randomized post test with control group” pada tikus yang di intervensi luka bakar pada punggungnya. Random alokasi dilakukan untuk membagi tikus Sprague Dawley dalam 4 kelompok yaitu kelompok kontrol(K1) dengan diet biasa, kelompok restriksi kalori(P1), kelompok suplementasi omega 3(P2) dan kelompok kombinasi restriksi kalori dengan suplementasi omega 3 (P3). Data dianalisis dan diolah menggunakan uji hipotesis Kruskall Wallis - Post Hoc test Mann-Whitney. Hasil: Penelitian didapatkan perbedaan bermakna yang signifikan kadar TNF Alfa pada kelompok Kontrol dengan kelompok P1, P2, dan P3 dengan p < 0,001. Luas luka bakar didapatkan hasil bermakna signifikan antar kelompok dengan p < 0,001. Post Hoc test kadar TNF alfa dan luas luka bakar menunjukkan tidak ada perbedaan bermakna antara kelompok P2 dengan P3. Kesimpulan: Kombinasi pemberian suplementasi omega 3 dengan restriksi kalori dapat menurunkan kadar TNF Alfa dan memperkecil ukuran luas luka bakar secara bermakna namun tidak untuk restriksi kalori saja Kata Kunci: penyembuhan luka, Omega 3, Kalori Restriksi, TNF Alfa Background: Burns are clinical problem with complications that often cause morbidity and mortality. Omega 3 has an anti-inflammatory effect and calorie restriction has an anti-oxidant effect which is thougth to accelerate the healing process in wounds. The research aims to determine wheteher the combination of Omega 3 supplementation and calorie restriction is expected to help in the process of healing burns that can be viewed from the level of tumor necrosis factor alpha and large burn healing. Methods: This study an experimental study with design of “randomized post test with control group” in rats given burns to their backs. Random allocation was made to divide Sprague Dawley rats into 4 groups: the control group with a normal diet (K1), the calorie restriction group (P1), the omega 3 supplementation group (P2), and the combination of omega 3 with calorie restriction group (P3). Data were analyzed and processed using the Kruskall Wallis - Post Hoc Mann-Whitney hypothesis test. Results: The study found significant differences in levels of TNF Alpha in the control group with groups P1, P2, and P3 with p<0.001. Burn area showed significant results between groups with p<0.001. Post Hoc tests for levels of TNF alpha and burn area showed no significant diffrence between P2 and P3 groups. Conclusion: The combination of omega 3 supplementation with calorie restriction can significantly reduce TNF alpha levels and significantly reduce the area of burns but not for calorie restriction only. Keywords: wound healing, omega 3, calorie restriction, TNF Alpha
Co-Authors Abraham Talent Bawadi Sibarani Ardyarini, Hikmatunnisa Tri Asri, Hairul Astika Widy Utomo, Astika Widy Awal Prasetyo Bahrudin Cecilia Noviyanti Salim Dzaki Edmonda Setyawan Edi Dharmana Endang Mahati Erwin Wibowo Farmaditya EP Mundhofir Ferdy Kurniawan Cayami Fikri Taufiq Haruaki Ninomiya Hery Djagat Purnomo Hesti Triwahyu Hutami Ichiro Hisatome Ika Pawitra Miranti Intan Rahmania Eka Dini M Ali Sobirin Manda Petrina Martin Adhitya Subagio Masanari Kuwabara Meita Hendrianingtyas Muhammad Thifan Satyagraha Muniroh, Muflihatul Neni Susilaningsih Peili Li Qorina Putri Srisantoso Rahajeng, Herera Rayvita AN Meagratia Rebriarina Hapsari Reza Dian Pratama Reza Dian Pratama Rizky Aditya Fardhani Selamat Budijitno Sultana MH Faradz Udadi Sadhana, Udadi Udin Bahrudin Udin Bahrudin Udin Bahrudin Widyastuti, Esti Wiwik Lestari Yora Nindita Yuriz Bakhtiar