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All Journal Jurnal Tumbuhan Obat Indonesia Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) Jurnal Biologi Tropis Jurnal Pengabdian Magister Pendidikan IPA Sarwahita : Jurnal Pengabdian Kepada Masyarakat Jurnal Abdi Insani
Dyke Gita Wirasisya
Universitas Mataram
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Public Health Social Sciences Other

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EVALUASI DAN IDENTIFIKASI GOLONGAN SENYAWA POTENSIAL ANTIBAKTERI PADA DAUN DAN KULIT BATANG MIMBA (Azhadirachta indica A. Juss) TERHADAP Escherichia coli Arif Setiawansyah; Aliefman Hakim; Dyke Gita Wirasisya
Jurnal Tumbuhan Obat Indonesia Vol 11 No 2 (2018): Jurnal Tumbuhan Obat Indonesia
Publisher : Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (442.318 KB) | DOI: 10.22435/jtoi.v11i2.1003

Abstract

ABSTRACT Neem (Azadirachta indica A.Juss) is a plant that potentially developed for antibacterial agent for both the leaves and barks. The aims of this study were to compare the effectiveness of the antibacterial activity of neem leav es and stem barks extract and to identify the antibacterial compounds of the most active fractions. The extraction method was done using sonication method. Antibacterial activity was evaluated using wells solid diffusion method and TLC-Bioautography. Extract fractionation was conducted using liquid-liquid partitioning method. The chemical compounds of extracts and fractions were analyzed using TLC and GCMS. The result of sonication extraction obtained neem leaves oil (12,02%), leaves crude extract (4,3%) and stem barks crude extract (16,85%). The major chemical constituents of GCMS analysis are 2,3-Dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one (6,06%), L-proline,1-Acetyl-(CAS) Acetylproline (5,85%), 4-hydroxy-2-methyl-pyrrolidine-2-carboxylic acid (21,42%), 2,3-Dyhidrobenzofuran (2,69%), alpha-D-methylglucopyranoside (4,54%), palmitic acid (2,92%), Arabino-hex-1-enitol, 1,5-Anhydro-2-deoxy-(CAS) glucal (31,69%). Phytochemical screening of neem leaves oil, leaves and barks crude extract revealed the presence of alkaloids, flavonoids, phenols, saponins, triterpenoids, steroids and sterols. Antibacterial test results showed neem leaves oil was more effective than leaves and stem barks crude extract against Escherichia coli. The n-hexane fraction showed higher antibacterial activity than ethyl acetate fraction and ethanol fraction. Phytochemical screening of n-hexane fraction showed the presence of triterpenoids, steroids, sterols and phenols. Mimba (Azadirachta indica A.Juss) merupakan tanaman yang berpotensi dikembangkan sebagai antibakteri baik bagian daun maupun kulit batang. Penelitian ini bertujuan untuk membandingkan efektivitas antibakteri ekstrak daun dan kulit batang mimba terhadap Escherichia coli dan untuk mengidentifikasi golongan senyawa potensial antibakteri pada fraksi teraktif. Pengujian aktivitas antibakteri dilakukan dengan metode difusi padat menggunakan sumuran dan KLT-Bioautografi. Fraksinasi ekstrak dilakukan dengan metode partisi. Komponen kimia ekstrak dan fraksi dianalisis menggunakan KLT dan GCMS. Hasil ekstraksi sonikasi diperoleh minyak daun (12,02%), ekstrak kasar daun (4,3%) dan ekstrak kasar kulit batang (16,85%). Skrining fitokimia menunjukkan minyak daun, ekstrak kasar daun dan ekstrak kulit batang mimba mengandung senyawa alkaloid, flavonoid, fenolik, saponin, triterpenoid, steroid dan sterol. Komponen kimia mayor hasil analisis GCMS minyak daun mimba adalah 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one (6,06%), L-proline,1-Acetyl-(CAS)Acetylproline (5,85%), 4-hydroxy-2-methyl-pyrrolidine-2-carboxylic acid (21,42%), 2,3-dyhidrobenzofuran (2,69%), Alpha-d-methylglucopyranoside (4,54%), Asam Palmitat (2,92%), Arabino-hex-1-enitol, 1,5-anhydro-2-deoxy-(CAS)glucal (31,69%). Hasil uji antibakteri menunjukkan minyak daun lebih efektif menghambat pertumbuhan Escherichia coli dibandingkan dengan ekstrak kasar daun dan kulit batang. Fraksi n-heksan menunjukkan aktivitas antibakteri paling besar dibandingkan dengan fraksi etil asetat dan etanol. Hasil skrining fitokimia fraksi n-heksan menunjukkan adanya senyawa triterpenoid, steroid, sterol dan fenolik.
Pengaruh Dua Metode Pengeringan Pada Aktivitas Antibakteri Ashitaba (Angelica keiskei ) Terhadap Streptococcus mutans: Effect of Two Different Drying Methods on Antibacterial Activity of Ashitaba Againts Streptococcus mutans Dyke Gita Wirasisya; Yohanes Juliantoni; Wahida Hajrin
Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal) Vol. 4 No. 1 (2018): (March 2018)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (115.289 KB) | DOI: 10.22487/j24428744.2018.v4.i1.9629

Abstract

The aim of this study was to determine a change that occurs in total phenolic content (TPC) and antibacterial activity of ashitaba (Angelica keiskei) after dried using two different methods : sun and oven drying. The effectiveness of the drying methods was evaluated in term of total phenolic content (TPC) by using spectrophotometric assay with Folin-Ciocalteu reagent and antibacterial activity againts Streptococcus mutans by in vitro macrodilution assay. Oven drying at 60oC possessed high TPC (2,98 ± 0,0935 g EAG/100g) compared to sun drying method (1,72 ± 0,0142 g EAG/100g). Simillar pattern was also observed in antibacterial activity. Oven drying have higher antibacterial activity with the MBC (minimal bactericidal concentration) value of 0,5 mg/mL againts Streptococcus mutans. Therefore, sun drying is not suggested for drying method of ashitaba in terms of total phenolic content and antibacterial activity compared with oven drying methods.
Peningkatan Kesehatan Masyarakat Melalui Sosialisasi Penggunaan TOGA (Tanaman Obat Keluarga) Di Desa Tembobor Dyke Gita Wirasisya
Bahasa Indonesia Vol 15 No 01 (2018): Sarwahita : Jurnal Pengabdian Kepada Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (784.644 KB) | DOI: 10.21009/sarwahita.151.07

Abstract

Kegiatan pengabdian ini dilaksanakan di Desa Tembobor, Kabupaten Lombok Utara. Kegiatan ini bertujuan untuk meningkatkan kesehatan keluarga dan menekan biaya pengobatan konvensional dengan cara memanfaatkan penggunaan TOGA untuk pengobatan, selain itu kegiatan ini juga dilakukan untuk meningkatkan pemanfaatan pekarangan untuk menanam TOGA. Kegiatan pengabdian dilakukan dalam 2 tahap, tahap pertama yaitu sosialisasi pemanfaatan TOGA pilihan yang mudah didapatkan di lingkungan desa dan tahap kedua adalah melakukan penanaman TOGA pada salah satu pekarangan rumah warga untuk dijadikan percontohan. Hasil nyata yang didapatkan dari program ini adalah masyarakat Desa Tembobor mengetahui cara pemanfaatan TOGA dan mulai untuk melakukan penanaman
Ethnobotanical Study of Medicinal Plants Used to Treat Degenerative Disease in East Lombok Dyke Gita Wirasisya; Nisa Isneni Hanifa; Wahida Hajrin
Jurnal Biologi Tropis Vol. 20 No. 3 (2020): September - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v20i3.2119

Abstract

Degenerative diseases have become a complex problem around the world. Until now, degenerative diseases have become the biggest cause of death in the world. Approximately, 17 million people die early each year due to the global epidemic of degenerative diseases. Peoples in developing countries still rely on medicinal plants for primary health care. The knowledge for medicinal plant were passed down through generation with little written documentation. This study aimed to document the indigenous medicinal plants used for the treatment for degenerative diseases in East Lombok, Indonesia and to find leads on prospective plants for further ethnopharmacology research based on quantitative ethnobotany index. The ethnomedicinal data were collected through interview and discussion among local healers and plant collected with the help of local guide also herbarium was made to aid specimen confirmation. Ethnomedicinal data was analyzed using use value (UV) along with fidelity level (FL). A total of 20 informants (traditional healers) were interviewed and from the study we can documented the use of 63 plants for the treatment of degenerative diseases. The UV ranged from 0,05 (Sesbania grandiflora) to 0,35 (Carica papaya), while the highest FL (100%) was found for 29 species. Based on the ethnobotanical index (UV and FL) from each plant we can recommend 15 species for further ethnopharmacological study to determine their therapeutic effects and mechanism of action. This study revealed rich ethnomedicinal knowledge from the community in East Lombok for the treatment of degenerative disease. 
Application of Simplex Lattice Design Method on The Optimisation of Deodorant Roll-on Formula of Ashitaba (Angelica keiskei) Wahida Hajrin; Windah Anugrah Subaidah; Yohanes Juliantoni; Dyke Gita Wirasisya
Jurnal Biologi Tropis Vol. 21 No. 2 (2021): Mei - Agustus
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v21i2.2717

Abstract

Ashitaba is known to have antioxidant activity and gram-positive antibacterial activity that causes body odor. This is the potential activity for an active substance to be developed as deodorant. The appropriate formula is needed, so it is necessary to optimize the formula using the right method. This study aimed was to determine the application of the simplex lattice design method on the optimization of a deodorant roll-on formula of ashitaba extract. Ashitaba was extracted by the maceration method. The formula optimization design was determined using the simplex lattice design method by Design Expert®7.5.1. The components for optimization were the concentration of carbopol and concentration of TEA, and the optimization parameters were the spreadability test, sticky power, and pH test. The optimum formula of deodorant consists of 0.45% carbopol and 2.05% TEA. The responses of optimum formula obtained spreadability test 6.32 ± 0.33 cm, sticky power 44.67 ± 3.94 seconds, and pH 7.73 ± 0.17. These results meet the criteria for good preparation but need further testing related to the effectiveness of the preparation and the level of acceptance of the preparation by the user.
Sosialisasi Pemanfaatan TOGA Dan Pembuatan Sabun Batang Alami (Sabun Aloe vera) Kepada Murid Yayasan Generasi Muslim Cendekia di Lombok Tengah, Indonesia Anggit L. Sunarwidhi; Dyke Gita Wirasisya; Nisa Isneni Hanifa
Jurnal Pengabdian Magister Pendidikan IPA Vol 3 No 2 (2020): .
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (299.61 KB) | DOI: 10.29303/jpmpi.v3i2.513

Abstract

Yayasan Generasi Muslim Cendekia (Yayasan GMC) terletak di desa Puyung, Lombok Tengah, Indonesia, dan telah memiliki fasilitas TOGA, namun selama ini tumbuh-tumbuhan obat yang terdapat di fasilitas tersebut tidak dimanfaatkan secara maksimal. Minimnya pengetahuan murid yayasan GMC yang menjadi alasan terjadinya hal tersebut. Oleh karena itu, dibutuhkan suatu program sosialisasi untuk mengedukasi murid di yayasan tersebut terkait hal ini. Selain itu, program sosialisasi juga melibatkan praktek pembuatan sediaan sederhana dengan pemanfaatan tanaman obat, yaitu pembuatan sabun batang alami dengan menggunakan aloe vera sebagai bahan utamanya. Sabun ini sangat bermanfaat terutama di masa pandemi COVID-19.Hasil evaluasi kegiatan menunjukkan bahwa kegiatan sosialisasi ini telah berjalan dengan baik, ditunjukkan dengan adanya peningkatan nilai posttest peserta yang signifikan dibandingkan dengan nilai pretest peserta. Melalui kegiatan ini, peserta mendapatkan pengetahuan terkait pemanfaatan TOGA maupun cara pembuatan sabun bantang alami (sabun aloe vera). Peserta menunjukkan antusias yang tinggi pada saat praktek maupun diskusi. Diharapkan ke depannya sosialisasi seperti ini dapat terus berlanjut, sehingga dapat terus meningkatkan kreatifitas murid-murid yayasan GMC dalam pemanfaatan TOGA, yang tidak hanya bermanfaat untuk kesehatan tetapi juga dapat berpotensi meningkatkan taraf ekonomi warga desa tersebut.
PELATIHAN PENANGANAN STREPTOCOCCUSIS PADA IKAN NILA (OREOCHROMUS NILOTICUS) MENGGUNAKAN PAKAN FERMENTASI DI DESA GONTORAN LINGSAR Fariq Azhar; Dyke Gita Wirasisya
Jurnal Abdi Insani Vol 6 No 2 (2019): Jurnal Abdi Insani Universitas Mataram
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v6i2.240

Abstract

Ikan nila (Oreochromis niloticus) merupakan salah satu jenis ikan yang banyak dibudidayakan di Indonesia. Ikan ini memiliki tingkat ekonomis yang cukup tinggi dan disukai konsumen. Selain itu, ikan ini mudah untuk dipelihara dan pertumbuhannya relatif lebih cepat. Upaya untuk meningkatkan produksi budidaya ikan ini masih terkendala dengan adanya serangan penyakit Streptococcosis terutama oleh bakteri Streptococcus agalactiae yang merupakan penyakit pada ikan nila yang biasa dihadapi oleh pembudidaya dan dapat menyebabkan kematian yang massal pada ikan budidaya. Untuk mengatasi penyakit bakterial biasanya digunakan antibiotik, namun pemberian antibiotik secara terus menerus dapat mengakibatkan terjadinya resistensi bakteri terhadap jenis antibiotik tersebut. Selain itu, efek samping pemberian antibiotik dapat meninggalkan residu yang nantinya akan membahayakan manusia dan lingkungan. Alternative yang dapat digunakan untuk mengatasi resistensi antibiotik adalah dengan memanfaatkan bahan alami dengan menggunakan pakan fermentasi. Penggunaan pakan fermentasi pada ikan diharapkan membantu meningkatkan sistem imun dari ikan tersebut. Penggunaan pakan fermentasi jauh lebih ramah lingkungan, lebih ekonomis, dan tidak terpapar residu yang berbahaya. Manfaat penyuluhan ini diharapkan akan mampu mendorong masyarakat untuk dapat menangani dan mencegah penyebaran penyakit pada ikan nila dan meningkatkan kemampuan masyarakat dalam pembuatan pakan fermentasi ikan yang dapat meningkatkan sistem imun ikan, sebagai alternative pengganti antibiotik Sehingga dapat membantu pembudidaya untuk mengurangi biaya produksi dan meningkatkan keuntungan dalam produksinya.
Anti-Stapylococcus Epidermidis of Methanolic Extracts from Some East Lombok Medicinal Plants Dyke Gita Wirasisya; Amni Hamid; Muhamad Haikhal; Agriana Rosmalina Hidayati; Anggit Listyacahyani Sunarwidhi; Nisa Isneni Hanifa
Jurnal Biologi Tropis Vol. 23 No. 2 (2023): April-June
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i2.4732

Abstract

Wounds are structural and functional disruptions of the skin that occur as a result of an injury. Wound healing is a complex tissue repair or remodelling process in response to the injury. The most common factor that causes wounds not properly heal is infection. An infection develops when microorganisms enter the body, multiply, and trigger an immune reaction in the body. This study aims to determine the activity of Jatropha multifida L., Stachytarpheta jamaicensis (L.) Vahl, Centella asiatica (L.) Urb., Euphorbia pulcherrima Willd. ex Klotzsch, and Angelica keiskei (miq.) Koidz has long been used to treat and manage wounds in East Lombok. The plants were dried and macerated with methanol; excessed solvent was evaporated. Disc diffusions were used to determine the antibacterial activity of the plant extracts. All extracts were tested against Staphylococcus epidermidis, a common cause of wound infection. Statistically, the activity of Jatropha multifida (5;10 mg/mL), Stachytarpheta jamaicensis (10 mg/mL), Centella asiatica (10 mg/mL), Euphorbia pulcherrima (M) (5, 10 mg/mL), and Angelica keiskei (2,5; 5; 10 mg/mL) extracts were comparable with positive control. However, Angelica keiskei has a wider inhibition zone than other extracts. This discovery could be served as a basis for using plants to aid wound healing, especially to combat the interference bacteria. However, further research is needed to discover the active phytochemicals involved in the antibacterial and wound healing process.
Co-Authors Aliefman Hakim Amni Hamid Arif Setiawansyah Fariq Azhar Hidayati, Agriana Rosmalina Muhamad Haikhal Nisa Isneni Hanifa Sunarwidhi, Anggit Listyacahyani Wahida Hajrin Windah Anugrah Subaidah Yohanes Juliantoni