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All Journal Jurnal Kesehatan Masyarakat Dental Journal (Majalah Kedokteran Gigi) Conservative Dentistry Journal

Widya Saraswati

Department Of Conservative Dentistry Faculty Of Dental Medicine, Universitas Airlangga

Author-ID : 3196156

Dentistry Public Health

Published : 22 Documents Claim Missing Document

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TENSILE STRENGTH OF BONDING MATERIAL TO DENTINE USING WATER-WET AND ETHANOL-WET TECHNIQUES Ainin Nafilatus; M. Mudjiono; Widya Saraswati
Conservative Dentistry Journal Vol. 8 No. 1 (2018): January - June
Publisher : Universitas Airlangga

Background: Composite resins have hydrophobic properties so it cannot attach to dentine. Moist dentin is a normal condition caused by the presence of fluid in dentinal tubules. Therefore bonding material is required to bond dentine with composite. Water-wet technique is a technique in which dentine surface is rinsed after etching by using water and allowing it to be moist and prevent the collapse of dentine collagen fibrils. This technique is commonly used today, but excess water disturbs the effectiveness of dentine and bonding material attachment. Ethanol-wet technique is a technique in which ethanol is used to replace water before bonding to reduce excess water in dentine surface. This technique will initiate monomer penetration into interfibrillar space of dentine. It will affect the result of tensile strength test. Purpose: The purpose of this study was to compare tensile strength of bonding material to dentine using water-wet and ethanol-wet techniques. Method: 12 samples of bovine incisors teeth were randomly divided into 2 group. Each group consists of six samples. Group 1 was treated with water-wet technique and group 2 was treated with ethanol-wet technique. Autograph was used as a tensile strength test instrument. Result : The average of tensile strength using water-wet technique was 2,16067 MPa and ethanol-wet technique was 4,21900 MPa. Conclusion: Ethanol-wet technique have higher value of tensile strength than water-wet technique.

ANTIBACTERICAL POTENCY OF CALCIUM HYDROXIDE AND PROPOLIS MIXTURE ON MIXED BACTERIA OF CARIOUS DENTIN Ruslan Effendy; Vita Ariesta; Widya Saraswati
Conservative Dentistry Journal Vol. 9 No. 1 (2019): January - June
Publisher : Universitas Airlangga

Background: Calcium hydroxide has been known as the gold standard of pulp capping process, but still has some weaknesses such as a pH that is too high to be potentially toxic, resistant to certain bacteria, causing necrosis of the pulp, and causing a tunnel defect. This weakness causes researchers to look for alternative ingredients that have antibacterical properties that can be combined with calcium hydroxide. Propolis has antibacterial properties such as flavonoids, tannins, saponins, and terpenoids which can work as the best carrier that allows dissociation of ions from calcium hydroxide so the combination of both that has antibacterial power is expected to have an effective antibacterial potency against the number of bacterial colonization with mixed dentine carious. Aims: This study aims to determine the antibacterial potency produced by a combination of calcium hydroxide and propolis against mixed bacteria of carious dentine. Methods: The research was carried out using 4 treatment groups with each of 7 samples. Group I is a combination of calcium hydroxide and propolis with a ratio of 1: 1, group 2 with a ratio of 1: 1.5, group 3 with a ratio of 1: 2, and a positive control group. Each sample was put into a suspense of mixed bacteria of carious dentine, grown for 24 hours. Suspense is vortexed for 1 minute so the solution becomes homogeneous. Put 0.1 ml of suspended mixed bacteria of dentine carious from BHIB media, grown for 24 hours on MHA media and counted the number of colonies. Results: The highest number of colonies in the positive control group, then group 1, group 2, and the least number of colonies in group 3 with the most extract of propolis. Conclusion: The combination of calcium hydroxide and propolis with a ratio of 1: 2 has the best antibacterial power in inhibiting colonization of mixed bacteria of carious dentine.

Effect of 405 nm Diode Laser with Varying Irradiation Time on BHK-21 Fibroblast Viability Kun Ismiyatin; Leidy Herlin Rumbiak; Widya Saraswati; Sri Kunarti; Anuj Bhardwaj
Conservative Dentistry Journal Vol. 9 No. 1 (2019): January - June
Publisher : Universitas Airlangga

Background: Laser is a device that emits light through a process of optical amplification based on the stimulated emission of electromagnetic radiation; it can be used for surgery, detoxification, bio stimulation and antibacterial. However, lasers have Biphasic Dose Response (BDR), which is bio stimulation and bio inhibition. To determine if 405 nm laser diode is biocompatible, viability test is necessary before these lasers can be labeled as viable to use in dental therapy. Aim: To prove the variation of radiation time of the 405 nm laser diode radiation can cause bio stimulation and bio inhibition response that affects the viability of BHK-21 fibroblast cells. Method: Viability test was carried out using BHK-21 fibroblast cells which were inserted into 96-well microplate, then radiated with 405 nm laser diode with varying irradiation time of 30s, 60s, 120s, 240s and 480s. After radiation, the cells are then incubated for 24h. Cytotoxicity was observed using MTT assay and ELISA reader. Data was analyzed using the Kolmogorov-Smirnov test, Levene Test, Welch ANOVA, and Tukey HSD. Results: BHK-21 fibroblast cells radiated with 405 nm laser diode with radiation time of 30s, 60s, 120s, and 240s have the same viability as the control cell, while at 480 seconds the viability exceeds that of the control cell. Conclusion: 405 nm laser diode with radiation times of 30s, 60s, 120s, and 240s do not affect the viability of BHK-21 fibroblast cells. Meanwhile, 480s irradiation time of 405 nm laser diode causes bio stimulation response that increases the viability of BHK-21 fibroblast cells.

Viability Test of Photodynamic Therapy with Diode Laser Waves Length 405 nm on BHK-21 Fibroblast Cells with Various Irradiation Distances Sri Kunarti; Vina Zavira Nizar; Widya Saraswati
Conservative Dentistry Journal Vol. 9 No. 2 (2019): July - December
Publisher : Universitas Airlangga

Background: Photodynamic therapy has now become popular, but its cytotoxic effect is still unclear. In order to be considered suitable for oral cavity therapy, the therapy must not be toxic or cause adverse effects on the target tissue. Viability testing for photodynamic therapy is important to do. Fibroblast cells are often used for testing the toxicity of dentistry because they are the most important cells in the components of the pulp, periodontal ligament, and gingiva. Purpose: To prove the effect of irradiation distance on photodynamic therapy on the viability of BHK-21 fibroblast cells. Method: Viability test was performed with BHK-21 fibroblast cells placed on a 96 well microplate which was then irradiated with 405 nm photodynamic therapy with varying irradiation distances of 1, 4, 7, 10, 13, and 16 mm. After irradiation, cell viability was tested by MTT assay and ELISA Reader. Data were analyzed using Kolmogorov-Smirnov, Levene’s test, Kruskall Wallis, and Tukey HSD. Result: Fibroblast cells with 4 mm irradiation distance have viability over control cells, whereas at irradiation distances 1, 7, 10, 13, and 16 mm have less viability than control cells. Conclusion: Photodynamic therapy 405 nm with 4 mm irradiation distance gives a biostimulation response so that the viability of BHK-21 fibroblast cells increases.

The Effect Of Self-Etch And Total-Etch Bonding Systems Application On Microleakage Of Bulkfill Flowable Composite Restoration In Carbonated Drink Immersion Widya Saraswati; Auryn Thania Song Hadinata; Sukaton Sukaton
Conservative Dentistry Journal Vol. 9 No. 2 (2019): July - December
Publisher : Universitas Airlangga

Background: Resin composite is more advanced today, but the marginal leakage still can not be avoided, for it takes the bonding system. Bonding system aims to provide a layer between a substrate and composite so it has a strong bond . The current latest bonding system is called universal bonding. It can be used with 2 techniques, both self etch and total etch. Universal bonding contains 10MDP so there’s no need for etching and priming. 10MDP results in better adhesion than previous bonding monomers generation. A total etch technique require a pre-etching step to remove smear layer so that it formed porous as a retentive sites. Purpose : Distinguish the marginal leakage of flowablebulkfill composite overlays with the application of total etch and self etch bonding systems accompanied by immersion of carbonated beverages . Method: Tooth samples were prepared to a depth of 2mm, then the bonding agent was applied directly to the cavity without etching (self etch), etched before the application of bonding agent (total etch ), directly loaded without pre-etching and bonding (control) . using a flowablebulkfill composite as a material restorative. All of the sample both total etch and self-etch soaked in Cola drinks for 5 minutes then repeated until 10 treatment each day (one day is assumed to be 12 hours) up to 7 days, followed by immersion in the methylene blue 0.5 % for 24 hours . Sample then splittedbuccolingual direction, leakage was assessed by scoring the edge trough the depth of methylene blue penetration. Result: Mann-Whitney Test showed a significant differences (P>0.05 ) each group . Conclusion: Marginal leakage of composite restoration with total etch technique and self etch technique is smaller than the control group, and the marginal leakage of composite restoration with total etch technique is smaller than that of self etch group .

The Duration Effectivity of Diode Laser 405 nm with Erythrosine Photosensitizer in Killing Streptococcus Mutans Sri Kunarti; Anin Dita; Widya Saraswati
Conservative Dentistry Journal Vol. 10 No. 1 (2020): January-June
Publisher : Universitas Airlangga

Introduction: Dental caries is a multifactorial disease caused by the interaction of pathogenic bacteria, especially Streptococcus mutans. Various caries prevention chemically and mechanically can help reduce the number of bacteria. However, this approach does not cover the entire bacterial population with a photodynamic therapy approach with the addition of photosensitizers and low-intensity light radiation with the right wavelength able to eliminate the number of S. mutans bacteria. Purpose:To determinethe duration of effective irradiation time on a 405 nm diode laser with erythrosine photosensitizer in killing S. mutans bacteria.Methods: This research was an experimental laboratory type with 25 S. mutans samples divided into 5 groups treated with erythrosine photosensitizer 0.1 mg / ml and 405 nm diode laser irradiation with 45 seconds, 60 seconds, 75 seconds duration and no erythrosine photosensitizers and without irradiation. The growth of S. mutans bacteria was calculated then the data were analyzed statistically.Results:the research found that S. mutans bacteria decreased gradually in each group; erythrosine without photosensitizer and without irradiation had the highest average growth rate of 71.6 CFU / ml; without photosensitizer with irradiation of 40.6 CFU / ml; irradiation with photosensitizer 45 seconds at 20.6 CFU / ml; irradiation with 60 seconds photosensitizer at 11 CFU / ml; and irradiation with 75 seconds photosensitizer at 0 CFU / ml. In statistical tests, the data are normally distributed and homogeneous. There are significant differences between groups.Conclusion: The effective duration in this study was found at 75 seconds.

The inhibitory efficacy of flavonoid of mangosteen peel extract (Garcinia Mangostana Linn.) against Lactobacillus Acidophilus biofilm bacteria Dyah Utari Wahyu Ningrum; Karlina Samadi; Widya Saraswati
Conservative Dentistry Journal Vol. 10 No. 2 (2020): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v10i2.2020.75-79

Background: Lactobacillus takes role in the formation of dental plaque which is the main factor that cause dental caries. The bacteria take role in the metabolism of glucose in the mouth, producing organic acids which lower the pH in the oral cavity. This situation can lead to the formation of dental caries because these bacteria can form biofilm as a defense of microorganisms to antibiotics and the immune response. Mangosteen peel has active ingredients such as flavonoid that can be used to inhibit biofilm. The ability of flavonoid compounds in the phenol group can make the bacterial enzyme becomes inactive, causing the activity of glucosyltransferase enzyme that usually used by bacteria to synthesize sucrose in the medium becomes glucan. As a result, bacterial biofilm formation is inhibited because the amount of glucan as a medium of bacterial attachment is limited, but until now the effectivity dose of flavonoid from mangosteen peel extract (Garcinia mangostana Linn.) is has not known yet Purpose: The aim of this study was to determine the inhibitory efficacy of flavonoid of mangosteen peel extract against the formation of Lactobacillus acidophilus bacterial biofilm. Methods: Bacteria that had been formed into biofilms was studied in two times treatment, one concentration reviewed by flavonoid from mangosteen peel extract and without reviewed by flavonoid from mangosteen peel extract. The treatment group was incubated at 37°C until day 8 since the first day incubated. The treatment group was washed with phosphate buffered saline (PBS) 4 times and dried. The treatment group stained with 0.2 ml 0.1% crystal violet and 15 min incubation. The treatment group was rinsed with distilled water 3 times and then dried. Solvent DMSO 100% was added as much as 0.1 ml in each well. Microtiter plate was shaken for 1 minute and then placed in to the microplate reader and then the OD (Optical Density) can be read. Results: There were significant differences between the inhibitory efficacy of flavonoid of mangosteen peel extract against Lactobacillus acidophilus biofilm bacteria, the control group and group treatments (p <0.05). Conclusion: Flavonoid of mangosteen peel extract with the concentration of 0.78% had inhibitory effect against Lactobacillus acidophilus biofilm bacteria

The Role of Propolis in Pulp Pain by Inhibiting Cyclooxygenase-2 Expression Ira Widjiastuti; Widya Saraswati; Annisa Rahma
Conservative Dentistry Journal Vol. 11 No. 1 (2021): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v11i1.2021.11-18

Background: Inflammation of the pulp can lead to elicit pain. Pain in inflammation is induced by the cyclooxygenase-2 enzyme (COX-2) which induces prostaglandin E2 (PGE2) resulting in pain. Pain in the pulp can be relieved by eugenol. In its application, eugenol is toxic to pulp fibroblasts. Due to the side effect, it is worth considering other biocompatible materials with minimal side effects, such as propolis. Flavonoids and phenolic acids that contained in propolis can inhibit COX-2. Therefore, an analysis outlined in the literature review is needed to examine the results of research related to the role of propolis as pulp pain relief by inhibiting COX-2 expression. Purpose: To analyze the role of propolis in pulp pain by inhibiting COX-2 expression. Reviews: Propolis extract that extracted by ethanol, water, and hydroalcohol has pain relief properties in the pulp by inhibiting COX-2 by directly binding to the COX-2 receptors and by reducing the production of proinflammatory cytokines which are COX-2 inducers, proven through in vivo, in vitro, and in silico studies in various target cell organs. Conclusion: Propolis extract has high prospect as inflammatory pain inhibitor in the pulp by inhibit COX-2 expression.

Comparison Adhesive strength of Resin Composite between Total-etch and Self-etch Techniques on Enamel After Fluoride Application Widya Saraswati; Adioro Soetojo; Yasmin Tasya Brilyanti
Conservative Dentistry Journal Vol. 11 No. 1 (2021): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v11i1.2021.38-41

Background : White Spot Lesion (WSL) is caused by Streptococcus Mutan which causes demineralization of teeth. One of the treatments of WSL is topical application of fluoride which aims to remineralize the teeth. However, if topical fluoride treatment does not give satisfactory results, alternative treatments can be made using restorative treatments, one of which is veneers. The procedure for applying veneers requires the application of an adhesive system. The adhesive system used to apply veneers is self-etch and total-etch. Several references state that the application of fluoride which aims to remineralize teeth can influence the adhesiv strength of the restorative material using self etch and total-etch techniques. Purpose : To determine the effect of composite resin adhesion strength between total-etch and self etch techniques on enamel after fluoride application. Review : Literature sources used in the preparation of the article through several databases with descriptions of the effects of fluoride application before total-etch and/or self etch administration. From the existing references, it was found that the application of fluoride before the total-etch system had a less significant effect on the adhesiv strength. Whereas in the self etch system, fluoride application has a significant effect on the adhesiv strength. Conclusion : More references say that the application of fluoride before the total-etch system has a better adhesive strength than the application of fluoride before the self etch system

White pomegranate (Punica granatum) peels extract bactericidal potency on Enterococcus faecalis Eric Priyo Prasetyo; Widya Saraswati; Dian Agustin Wahjuningrum; Latief Mooduto; Rizka Firdaus Rosidin; Evelyn Tjendronegoro
Conservative Dentistry Journal Vol. 11 No. 2 (2021): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v11i2.2021.84-88

Background: Enterococcus faecalis (E. faecalis) is the most resistant bacteria in the root canals and one of the causes of recurrent endodontic treatment. Enterococcus faecalis was not the only bacteria found in failed endodontic treatment but these bacteria can thrive in unfavorable environment. White pomegranate (Punica granatum) is known for its flavonoids and tannins that function as antimicrobial agent. White pomegranate extract is potential for use as disinfection or irrigation material. Purpose: This aim of this study was to determine the bactericidal potency of white pomegranate (Punica granatum) extract on E. faecalis growth. Methods: This study was an in vitro experimental observation. E. faecalis was obtained from stock culture taken from the root canal of recurrent endodontic treatment. E. faecalis from the serial dilution were cultured in blood agar media. Antibacterial potency was determined by colony calculation of E. faecalis growth in blood agar in colony forming unit (CFU) and conducted in 6 replications for each concentration group. Statistical analysis was done using one-way analysis of variance at 5% significance level. Results: White pomegranate peels extract concentrations of 3.125%, 6.25%, 12.5% and 25% provide significant decrease in the number of E. faecalis colony compared to the control group (p<0.05). No bacterial growth was found on 25%, 50% and 100% concentration. Conclusion: The potent minimal bactericidal concentration of white pomegranate peels extract on E. faecalis was 25%.

Co-Authors Adioro Soetojo Ainin Nafilatus Alvita Wibowo Anggi Puspitasari Anin Dita Annisa Rahma Annisa Salsabila Andika Putri Anuj Bhardwaj Auryn Thania Song Hadinata Dewi Kusuma Wardani Dian Agustin Wahjuningrum Dian Pramita Ayu Kumalasari Dur Muhammad Lashari Dyah Utari Wahyu Ningrum Ekawati Ekawati Eric Priyo Prasetyo Erin Imaniar B Evelyn Tjendronegoro Gabriela Kevina Alifen Ida Wahyuni Ira Widjiastuti Karlina Samadi Ketut Suardita Kun Ismiyatin Latief Mooduto Leidy Herlin Rumbiak M. Mudjiono Nadhifa Salma Nirawati Pribadi Rizka Firdaus Rosidin Ruslan Effendy Ruslan Effendy Sekar Firdhea Rizkifa Soetanto Sri Kunarti Sukaton Sukaton Tasya Nafatila Veda Sahasika Azaria Nariswari Vina Zavira Nizar Vita Ariesta Yasmin Tasya Brilyanti Yovita Yonas

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