KIKI NURTJAHJA
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Sumatera Utara Jl. Bioteknologi No.1, Kampus USU, Medan, Indonesia 20155

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PENGHAMBATAN SERANGAN Sclerotium rolfsii PENYEBAB REBAH KECAMBAH PADA KEDELAI DENGAN BAKTERI KITINOLITIK Novi Malinda; Dwi Suryanto; Kiki Nurtjahja
Saintia Biologi Vol 1, No 1 (2012)
Publisher : Saintia Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (504.203 KB)

Abstract

The inhibition of Sclerotium rolfsii as causal agent of soybean damping off by using chitinolytic bacteria were studied in Laboratory of Observation Pest and Disease UPT-BPTPH 1 and Laboratory of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Sumatera Utara, Medan. The aim of this research was to investigate  the ability of chitinolytic bacteria to inhibit the growth of S. rolfsii. Six isolates of chitinolytic bacteria were used in this study. The inhibition zone between colony of the bacteria and the fungus was measured. The result showed that the bacterial isolates has different ability in inhibiting the growth of S. rolfsii. Enterobacter sp. BK15 and Bacillus sp. BK13 were the most effective in inhibiting the growth of S. rolfsii with inhibition zone of 3.70 cm and 3.75 cm, respectively. The less effective was shown by Enterobacter sp. PB17 with inhibition zone of 0.10 cm, while Enterobacter cloacae LK08 have no ability to inhibit S. rolfsii. The seeds soaked in bacterial suspension by  Bacillus sp. BK13 dan Enterobacter sp. BK15 were able to reduce damping off by 44.4% and 50%, respectively.   Keywords: Bacillus sp., damping off, Enterobacter sp., Sclerotium rolfsii, soybean
KEMAMPUAN BAKTERI ENDOFIT TANAMAN SEMANGKA DALAM MENEKAN PERKEMBANGAN PENYAKIT BERCAK DAUN YANG DISEBABKAN OLEH JAMUR COLLETOTRICHUM SP. Eryna Elfasari Rangkuti; Dwi Suryanto .; Kiki Nurtjahja .; Erman Munir .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 14 No. 2 (2014): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (408.67 KB) | DOI: 10.23960/j.hptt.214170-177

Abstract

Ability of watermelon endophytic bacteria to suppress development of leaf spot disease caused by Colletotrichum sp. A studi on assay of endophytic bacteria to control Colletotrichum sp., causal agent of leaf spot disease on watermelon, was conducted. Colletotrichum sp. was isolated from infected leaf of leaf spot disease, while endophytic bacteria were isolated from stem, leaf, and root of watermelon healthy plant. Antagonistic assay was conducted by dual culture method. Hyphal abnormalities as a result of antagonistic assay was observed using light microscope. To determine the ability of endophytic bacterial isolates to control leaf spot disease, watermelon seeds were treated by dipping the seed in endophytic bacterial suspension. Seven endophytic bacterial isolates showed to inhibit Colletotrichum sp. to some extent. Two isolates DS 01 and BS 01 showed relatively high inhibition zone compared to others, therefore were choosen for further study. Abnormal hyphae such as broken, lysis, twisted, curled, and swollen hyphae were recorded as the result of antagonistic assay. Watermelon seed treatment revealed that dipping the seed in endophytic bacterial suspension of DS 01 and BS 01 reduced leaf spot disease to 12 and 24%, but inhibited seed growth by 12% and 44%, respectively. It seemed that all treatments showed to reduce seedling performance i.e seedling height, leaf number, and dry weight, compared to that of (-) control. DS 01 however seemed to increase dry weight of watermelon plants.
ASAI KEMAMPUAN BAKTERI ENDOFIT DARI KACANG TANAH DALAM MENGHAMBAT PERTUMBUHAN Sclerotium sp. PADA KECAMBAH KACANG TANAH Lisa Novita Arios; Dwi Suryanto .; Kiki Nurtjahja .; Erman Munir .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 14 No. 2 (2014): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (886.632 KB) | DOI: 10.23960/j.hptt.214178-186

Abstract

Assay on ability of endophytic bacteria isolated from peanut to inhibit Sclerotium sp. growth in peanut seedlings. A study on assay of ability of endophytic bacteria to inhibit Sclerotium sp. in peanut seedling has been done. The bacteria were isolated from peanut healthy plants, while Sclerotium sp. was isolated from infected peanaut plant. Antagonistic assay was conducted by dual culture method. In vivo assay of inhibiting Sclerotium sp. was conducted by dipping peanut seed in bacterial solution, and planting the seed in soil:compost (3:1) growing media. Six endophytic bacterial isolates showed to inhibit the growth of Sclerotium sp. in vitro. LN1 seemed to inhibit more of Sclerotium sp., while LN5 showed to inhibit less. Two potential isolates LN1 of gram-negative and LN2 of gram-positive using for further study showed to decrease more of dumping off. It also seemed that the isolates increased the seedling height, number of leaves, and dry weight.
Pengaruh Konsentrasi Air Kelapa dan Air Cucian Beras dan Lama Perendaman Terhadap Perkecambahan Benih Kacang Hijau (Vigna radiata L.) Srimaulinda Srimaulinda; Kiki Nurtjahja; Riyanto Riyanto
Jurnal Ilmiah Biologi UMA (JIBIOMA) Vol 3, No 2 (2021): November
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/jibioma.v3i2.751

Abstract

Air kelapa adalah senyawa alami kompleks yang sering digunakan sebagai pengatur tumbuh alami.   Penggunaan air kelapa sebagai bahan organik merupakan alternatif dari penggunaan bahan sintetis pada tanaman. Tujuan penelitian ini adalah untuk mengetahui pengaruh konsentrasi limbah dalam air kelapa dan air cucian beras serta lama perendaman terhadap perkecambahan biji kacang hijau. Parameter yang diamati adalah tinggi globul, jumlah radikula, dan panjang radikula. Berdasarkan hasil penelitian, air kelapa dan air cucian beras berpengaruh terhadap tinggi bulu dimana bulu tertinggi terdapat pada kombinasi (20 ml air kelapa + 80 ml air cucian beras dan perendaman 2 jam). Panjang radikula terpanjang ditemukan pada kombinasi (10 ml air kelapa + 90 ml air cucian beras dan perendaman 6 jam). Jumlah akar paling banyak terdapat pada kombinasi (50 ml air kelapa + 50 ml air cucian beras dan perendaman 6 jam).
Infeksi Jamur Dermatofita Pada Penderita Mikosis Kuku Winda Irawati Zebua; Kiki Nurtjahja; Sartini Sartini
Jurnal Ilmiah Biologi UMA (JIBIOMA) Vol 3, No 1 (2021): Mei 2021
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/jibioma.v3i1.539

Abstract

Dermatophytes are a group of fungi that cause nail abnormalities. This study aims to determine dermatophyte fungal infections in patients with nail mycoses. The method used in this study is descriptive and the sample was taken by purposive sampling with the criteria of damaged toenails, with characteristics: nails are not shiny, brownish to black from the base to the tip then the nails are examined directly with 10% KOH and cultured using media Sabouraud Dextrose Agar (SDA). The fungi isolated on slanted agar containing SDA and each fungal species were then identified using preparations for microscopic examination. The results showed that from 30 samples, 8 (eight) nail samples were positive for dermatophytes, namely: 5 (five) people infected with the fungus Tricophyton mentagrophytes species, 2 (two) people infected with the Trichophyton rubrum fungus, and 1 (one) person infected with Epidermophyton floccosum..
Hydroponic Planting Training to Maintain Food Security of the People of Bandar Setia Village, Percut Sei Tuan District, Deli Serdang Regency, North Sumatra Miswar Budi Mulya; Kiki Nurtjahja; Mimmy Sari Syah Putri
ABDIMAS TALENTA: Jurnal Pengabdian Kepada Masyarakat Vol. 8 No. 1 (2023): ABDIMAS TALENTA: Jurnal Pengabdian Kepada Masyarakat
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/abdimastalenta.v8i1.10083

Abstract

The people of Bandar Setia Village experienced the impact of the implementation of PPKM due to regulations delimiting community activities. The limited agricultural land in the village also affects the fulfillment of food needs, especially vegetables. The purpose of this community service is to train the Forum Peduli Sesama Seniman Indonesia Cabang Tembung group representing the community of Bandar Setia in terms of hydroponic farming as an alternative to providing food stocks for the community in the village during the pandemic. The specific target of the training is the hydroponic model that can be made by the people of Bandar Setia village. The method used mixing counseling, training, hands-on practice, and work evaluation. Hydroponics manufacturing uses a mixing of NFT (nutrient film technique) and wick system methods. Vegetables harvested using hydroponics can be used for personal needs or sold according to the needs of the people of Bandar Setia village.