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All Journal Jurnal Fitopatologi Indonesia BALI MEDICAL JOURNAL (BMJ) Agrotrop : Journal on Agriculture Science International Journal of Biosciences and Biotechnology Buletin Udayana Mengabdi Journal of Agricultural Science and Biotechnology E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Jurnal Ilmu Lingkungan INTERNATIONAL JOURNAL OF APPLIED CHEMISTRY RESEARCH
Ni Made Puspawati
Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Energy Environmental Science Materials Science & Nanotechnology Medicine & Pharmacology

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Journal : International Journal of Biosciences and Biotechnology

 1 
UTILIZATION OF RHIZOSPHERE FUNGI TO CONTROL Fusarium oxysporum f.sp. capsici IN VITRO I Made Sudarma; Ni Made Puspawati; Ni Wayan Suniti; I Nyoman Wijaya; I Gusti Ngurah Bagus
International Journal of Biosciences and Biotechnology Vol 2 No 2 (2015)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

Fusarium caused wilt disease in chilli pepper and destroyed some farmer crops. Results ofpreliminary research has been discovered that the disease caused by Fusarium oxysporum f.sp. capsici.The alternative environmental friendly method is to find antagonist microbes which is located in therhizosphere of healthy pepper plants. This study aims to find out potentially antagonistic fungi tocontrol Fusarium wilt disease on pepper plants. The fungi were isolated by soil dilution method orviable plate count method on Potato Dextrose Agar medium with antibiotic Livoplaxacin (25%, w/v).Rhizosphere fungi from healthy pepper plants had been identified. A total of 63 spesies belong to 4genera included Penicillium (45 species), Aspergillus (6 species), Trichoderma (9 species) andCandida (3 species). The highest percentage of distribution of rhizosphere fungi are P. digitatum(47.63%), P. expansum (19,05%), T. harzianum (9,53%), A. nidulans, A. niger, Penicillium sp.,Candida albicans, and T. vitrens i.e 4,76% respectively. All of rhizosphere fungi colonies were foundto inhibit Fusarium oxysporum f.sp. capsici in vitro. The best inhibition was found in Aspergillusniger at 88.89 ± 2.2% followed by A. nidulans of 85,56 ± 1,6 %, T. harzianum at 84,45 ± 1,58% , andT. virens by 83,33 ± 1,2%, five days after inoculation. All of them have a very high inhibition criteria.
Co-Authors DWI WIDANINGSIH GEDE SUASTIKA Gusti Ngurah Alit Susanta Wirya I Dewa Nyoman Nyana I G. R. M. TEMAJA I Gusti Ngurah Bagus I GUSTI NGURAH BAGUS PRANATA PUTRA I Ketut Siadi I Ketut Suada I MADE SUDARMA I MADE SUDARMA I MADE SUDARMA I MADE SUDARMA I MADE SUDARMA I MADE SUDARMA I NYOMAN WIJAYA IDA BAGUS MADE DWI JAYA KETUT AYU YULIADHI Khairun Nisak Syahdu KHAMDAN KALIMI M SUDANA MADE SRITAMIN Michel Williams, Michel Muhammad Ikhsan Nulzaen NI NENGAH DARMIATI NI NYOMAN ALIT PURWANINGSIH NI NYOMAN ARI MAYADEWI NI PUTU MERRY SENI ANTARI NI WAYAN SUNITI NYOMAN RAI KUNTALINI OCTA FRANSISCA SITORUS ONGKY ARI WIBOWO PANDE MADE INDRAYOGA Putu Sudiarta Sinarsih, Ni Ketut Sri Wahjuni WIWIK SUSANAH RITA