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All Journal Jurnal Fitopatologi Indonesia BALI MEDICAL JOURNAL (BMJ) Agrotrop : Journal on Agriculture Science International Journal of Biosciences and Biotechnology Buletin Udayana Mengabdi Journal of Agricultural Science and Biotechnology E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Jurnal Ilmu Lingkungan INTERNATIONAL JOURNAL OF APPLIED CHEMISTRY RESEARCH
Ni Made Puspawati
Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Energy Environmental Science Materials Science & Nanotechnology Medicine & Pharmacology

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Journal : E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology)

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Uji Efektifitas Ekstrak Daun dari Beberapa Jenis Tanaman untuk Mengendalikan Nematoda Puru Akar Meloidogyne Spp. pada Tanaman Cabai (Capsicum Annum L) IDA BAGUS MADE DWI JAYA; MADE SRITAMIN; NI MADE PUSPAWATI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.2, April 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

ABSTRACT Root knot nematodes (Meloidogyne spp.) is an important class of nematode that causes significant reduction in the production of Capsicum annum L. Various control measures were taken to suppress the population of Meloidogyne spp. such as planting resistant varieties, crop rotation and technical culture, still unwell provide fast response compared to chemical control. Biopesticides is a way of controlling the use of more environmentally friendly than synthetic pesticides. Based on this, it will be carried out studies on the control of root knot nematodes Meloidogyne spp. using plant leaves tembelekan (Lantana camara), kirinyuh (Chromolaena odorata), papaya (Carica papaya), lemongrass (Cymbopogon nardus L.), betel (Piper betle L.) and tobacco (Nicotiana tabacum) which extracts used were 250cc / pots of each extract solution leaves. The purpose of this study was to determine the plant leaf extract is capable of suppressing the population of Meloidogyne spp. and determine the most effective leaf extracts suppress the population of Meloidogyne spp. The results of this empirically found that population of nematoda per 300 grams of soil extract of Piper betle L. can suppressed the nematode population of Meloidogyne spp. The best is 23.4 nematodes or 95.32%, followed by treatment of the extract of C. odorata 27.6 nematodes (94.48%), L. camara 28.8 nematodes (94.24%), Carica papaya 43, 4 nematodes (91.32%), Cymbopogon nardus L. 53.8 nematodes (89.4%), and Nicotiana tabacum 60.8 nematodes (87.84%). Population of nematoda per 1 g of root, extract of Piper betle Linn. is also the most well extract in suppressing root knot nematode populations, there are only recorded 21.2 percentage suppression tail with 95.76%, followed by treatment of C. odorata extract 23.6 tail (95.28%), L. camara 24 , 2 goats (95.16%), Carica papaya 28.4 tail (94.32%), Cymbopogon nardus (L). 30.2 tail (93.96%), and Nicotiana tabacum 35.2 tail (92.96%). Keyword: Capsicum annum L, Carica papaya, Chromolaena odorata, Cymbopogon nardus L., Lantana camara, Meloidogyne spp., Nicotiana tabacum, Piper betle Linn.
Pengaruh Inokulasi Trichoderma sp. Indigenus terhadap Penyakit Akar Gada dan Pertumbuhan Tanaman Kubis (Brassica oleracea L.) NI PUTU MERRY SENI ANTARI; NI MADE PUSPAWATI; I KETUT SUADA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.6, No.4, Oktober 2017
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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The Influence of Indigenous Trichoderma sp. Inoculation against Clubroot and the Growth of Cabbages (Brassica oleracea L.) The Cabbage (Brassica oleracea L.) is a plant that contains many vitamins and minerals needed by human. The problem which often experienced by cabbage farmers is clubroot deseases that caused by pathogenic Plasmodiophora brassicae Worr. Eco-friendly controls are needed so as not to adversely affect ecosystems by using natural enemies of the pathogen such as Trichoderma sp. The purpose of this research was to obtain the type Trichoderma sp. which effectively suppress clubroot and increase the growth of cabbage plants. The results showed that the most effective isolate was isolate from zucchini plant (Cucurbita pepo L.) rhizosphere (T1), followed by tomato plant (Lycopersicon esculentum L.) rhizosphere (T2), romana plant (Lactuca sativa L. var. longifolia) rhizosphere (T4), and from the spinach plant (Spinacia oleracea L.) rhizosphere (T9). The isolates were able to decrease clubroot and promote cabbage plant growth as well.
Pengaruh Penyakit Virus Mosaik dan Kuning Terhadap Hasil Panen Tanaman Kacang Panjang (Vigna sinensis L.) Di Desa Perean, Baturiti, Tabanan NI NYOMAN ALIT PURWANINGSIH; NI MADE PUSPAWATI; I DEWA NYOMAN NYANA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.5, No.3, Juli 2016
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Influence Mosaic Diseases and Yellow to Long Bean (Vigna sinensis L.)Production at Perean Village, Baturiti, TabananThis study aims to determine how far the influence of viral infection with symptoms of mosaic and yellow against a decrease in crop yield long beans. This research consists of four activities are: 1. Determining the spread of disease areas; 2. Observation and data collection; 3. Calculation yields; and 4. Data analysis. Design used was a randomized block design (RBD) with three treatments and nine replications.Research result show that long bean plants in several locations in Perean village infected by BCMV which has mosaic symptoms and MYMV which has yellow symptoms. Percentage of mosaic virus symptoms is higher than yellow virus. The average of percentage of mosaic virus symptoms was 42,85%, while yellow virus was 32,70%. Plant beans with yellow virus symptoms causes the highest yield loss which amounted to 53,87% and plants with symptoms of mosaic virus by 43,02% when compared with healthy plants. Based on these studies it is necessary dissemination of information, about the importance of sanitation and the use of healthy seeds to controlling the long bean’s viral infection to related parties.
Identifikasi Virus Yang Berasosiasi Dengan Penyakit Mosaik, Kuning, Dan Klorosis Pada Tanaman Cabai Rawit (Capsicum Frutescens L.) I GUSTI NGURAH BAGUS PRANATA PUTRA; NI MADE PUSPAWATI; I DEWA NYOMAN NYANA; I KETUT SIADI; GEDE SUASTIKA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.3, Juli 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

ABSTRACT Identification of virus that associated with Mosaic, Yellow, and Chlorosis disease on Chili Pepper (Capsicum frutescens L) This study aims to identify the types of viruses associated with mosaic, yellow, and chlorosis that infect the plants of chili pepper ( Capsicum frutescens L. ) in Kerta village, Gianyar. The method used is the ELISA (Enzyme Linked Immunosorbent Assay) and molecular techniques through PCR (Polymerase Chain Reaction). Research activities include (1) Survey endemic locations of viral diseases in Kerta village, Gianyar (2) Collecting leaf of chilli peper that shown, mosaic, yellowing and chlorosis (3) serology test by ELISA (4) molecular detection through PCR. The results show the percentage average of pepper plants showing mosaic symptoms (52.13%), yellow (22.75%), chlorosis (5.45%) and healthy plant (19.67%). Serology test by ELISA technique showed that the mosaic disease induced by the triple virus that is TMV, ChiVMV, and CMV, whereas yellow symptoms infected by PepYLCV and chlorosis symptoms, infected by Polerovirus. RT-PCR technique successfully amplify the target DNA fragment size of 650 bp for Polerovirus and PCR successfully amplified the target DNA fragment size of 700 bp for PepYLCV in accordance with the specific primers were used. Keywords : Chili pepper, mosaic , yellow, chlorosis
Identifikasi Jenis dan Populasi Jamur Tanah pada Habitat Tanaman Kubis (Brassica oleracea L.) Sehat dan Sakit Akar Gada pada Sentra Produksi Kubis di Kecamatan Baturiti Tabanan PANDE MADE INDRAYOGA; I MADE SUDARMA; NI MADE PUSPAWATI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.2, No.3, Juli 2013
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

ABSTRACT Identify Types and Population of Soil Fungi on Cabbage (Brassicae oleracea L.) Health and Infected Habitat of Clubroot in Baturiti District, Tabanan Cabbage is a vegetable that potential to be developed, because a lot of people favored this vegetable. Cabbage crop production level is often affected by the attacks of pathogen Plasmodiophora brassicae that causes swelling at the root. This disease can reduce the production of cabbage plants up to 80%. The aim of this research is to investigate species variety and soil fungi population on healthy cabbage plant’s rizosphere (suppressive) and diseased cabbage or diseased sympton (conducive) of club root (Plasmodiophora brassicae), and also soil fungi which more dominant on isolation result of soil fungi, to investigate the relation of soil’s physico-chemistry nature with soil fungi population, and to investigate fungi species that able to antagonize P. Brassicae fungi. This research used 3 samples of soil which taken from both healthy and club root diseased cabbage plant’s rizosphere, where the soil’s sample taken from 3 locations, that are: Pekarangan, Baturiti Kaja, and Pacung. The result of this research shown that there are 17 species of soil fungi found on the sample of suppressive and conducivesoil in three locations, where the population of Fusarium sp. fungi becomes dominant soil fungi. This research also shown that on the relation between soil physico-chemistry nature and soil fungi population was not positively correlated, and species fungi of Trichoderma sp. is able to inhibit the growth of P. Brassicaefungi. Keywords: Cabbage plant, Plasmodiophorabrassicae, species and population of soil fungi, suppressive and conducive soil.
Uji Daya Hambat Jamur Eksofit terhadap Phytophthora palmivora (Butler) Butler Penyebab Penyakit Busuk Buah Kakao secara In Vitro ONGKY ARI WIBOWO; I MADE SUDARMA; NI MADE PUSPAWATI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.6, No.3, Juli 2017
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

In Vitro Inhibition Test of Exophytic Fungi against Phytophthora palmivora (Butler) Butler the Cause Black Pod Disease on Cocoa Cocoa is one of the featured crop plantations in indonesia. In a development widely, a common problem is black pod disease. In indonesia Phytophthora palmivora fungi is a major cause black pod disease of cocoa. This disease can reduce the yield and quality of cocoa up to 32-99%.The use of microbial antagonists as a biocontrol agent to suppress the growth of P. palmivora is important. Some exophytic fungi known to have high potential antagonists in suppressing the growth of P. palmivora fungi. This study was aims to determine the potential inhibition of exophytic fungi in suppressing the growth of P. palmivora fungi in vitro. This research was conducted in July 2016 to October 2016 at the laboratory of Biotechnology, Faculty of Agriculture, University of Udayana. Stages of the research include: isolation of pathogenic fungi, isolation exophytic fungi, an in vitro test, and identification of fungi. The result showed the pathogen P. palmivora the cause black pod disease of cocoa at Perean Village, Tabanan Regency, Bali. Exophytic fungus which has the potential inhibition of the highest in study include: Rhizoctonia sp. 93,7%, Trichoderma sp1. 92,9%, Rhizopus sp. 94,4%, Aspergillus sp. 88,2%, Trichoderma sp2. 94,8%, Mucor sp. 93,7%. Exophytic fungi which has antibiosis substance is Trichoderma sp1.
Uji Antagonistik Beberapa Rizobakteri terhadap Sclerotium rolfsii Sacc. Penyebab Penyakit Rebah Kecambah pada Tanaman Kacang Tanah NYOMAN RAI KUNTALINI; KHAMDAN KALIMI; NI MADE PUSPAWATI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.2, April 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

ABSTRACT Antagonistic Test of Some Rhizobacteria Against Sclerotium rolfsii Sacc. Causes Damping Off Disease in Peanut Plant Damping-off disease is one of the important diseases on peanut plants caused by the fungi Sclerotium rolfsii Sacc. Control which is currently only with synthetic pesticides, which many negative impacts on ecosystems and humans. The many negative effects of pesticides would require alternative more environmentally friendly control. One alternative that can be recommended is the use of rhizobacteria which acts as a biological agent. The results showed that there were some rhizobacteria that effectively inhibit the growth of S. rolfsii Sacc. the inhibition test in vitro with the highest inhibition in the treatment of K. pneumoniae isolates KCX1GRA by 94.9% when compared with controls at 3 days after inoculation observation. Rhizobacteria filtrate tested in vitro showed that the treatment of the filtrate concentration of 10% - 20% on respectively filtrate rhizobacteria able to inhibit the growth of fungus S. rolfsii Sacc. Treatment of the filtrate rhizobacteria isolates 20M2 and KCX1GRA concentration of 20% -50% able to kill the fungi S. rolfsii Sacc., while the filtrate rhizobacteria isolates KCBS and Pi1 concentration of 30% -50% are also able to kill the fungi S. rolfsii Sacc. In the glass house study, the treatment was able to suppress rhizobacteria disease incidance in peanuts from 92% to 8% -10% compared with the control treatment was 92% at 6 weeks after planting observation. Keywords: rhizobacteria, biological agents, and S. rolfsii Sacc.
Deteksi Keberadaan Penyebab Penyakit Citrus Vein Phloem Degeneration (CVPD) pada Tanaman Jeruk dengan Gejala Menyeluruh Menggunakan Teknik Polymerase Chain Reaction (PCR) OCTA FRANSISCA SITORUS; I NYOMAN WIJAYA; NI MADE PUSPAWATI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.5, No.4, Oktober 2016
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Detection of Ocurrence Citrus Vein Phloem Degeneration (CVPD) Disease on Citrus Plant with Comprehensive Symptoms using Polymerase Chain Reaction (PCR) Technique Citrus Vein Phloem Degeneration (CVPD) is one of very serious disease of citrus plant. The disease is caused by Liberobacter asiaticum bacteria and transmitted by Diaphorina citri Kuw insect. The outspread of disease can also caused by the use of citrus seedlings which have been infected by CVPD. Preliminary visual observations CVPD disease symptoms, there are two types of plants showing symptoms of partial and systemic. Partial symptoms are not all the leaves of plants showing symptoms CVPD, while the systemic symptom is on the whole leaves of plants showing symptoms CVPD. This research aimed to determine the outspread of L. asiaticum bacteria in every parts of the plant that made detection by PCR. The research has been conducted from in Batukaang, Bangli and Plaga, Badung then continued at Laboratory of Genetic Resources and Biology Molecular, Udayana University. Results of PCR visualization using 1% agarose gel showed that there were a DNA band at 1160 bp on the leaves, twigs, branches and trunks. Because the size of 1160 bp DNA bands is expression by L. asiaticum, then the citrus leaf samples were detected positive for the L. asiaticum and it can be ascertained that L. asiaticum haas been distributed to all parts of the plant.
Co-Authors DWI WIDANINGSIH GEDE SUASTIKA Gusti Ngurah Alit Susanta Wirya I Dewa Nyoman Nyana I G. R. M. TEMAJA I Gusti Ngurah Bagus I GUSTI NGURAH BAGUS PRANATA PUTRA I Ketut Siadi I Ketut Suada I MADE SUDARMA I MADE SUDARMA I MADE SUDARMA I MADE SUDARMA I MADE SUDARMA I MADE SUDARMA I NYOMAN WIJAYA IDA BAGUS MADE DWI JAYA KETUT AYU YULIADHI Khairun Nisak Syahdu KHAMDAN KALIMI M SUDANA MADE SRITAMIN Michel Williams, Michel Muhammad Ikhsan Nulzaen NI NENGAH DARMIATI NI NYOMAN ALIT PURWANINGSIH NI NYOMAN ARI MAYADEWI NI PUTU MERRY SENI ANTARI NI WAYAN SUNITI NYOMAN RAI KUNTALINI OCTA FRANSISCA SITORUS ONGKY ARI WIBOWO PANDE MADE INDRAYOGA Putu Sudiarta Sinarsih, Ni Ketut Sri Wahjuni WIWIK SUSANAH RITA