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PENGARUH PEMBERIAN JAMUR Fusarium graminearum TERHADAP HISTOPATOLOGI TUBULUS SEMINIFERUS MENCIT (Mus musculus) Fierda Kabayo; Abdul Samik; Ismudiono Ismudiono; Tjuk Imam Restiadi; Soeharsono Soeharsono; Hani Plumeriastuti
Ovozoa : Journal of Animal Reproduction Vol. 8 No. 1 (2019): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (598.727 KB) | DOI: 10.20473/ovz.v8i1.2019.54-60

Abstract

The aim of this research was to show the influence of seminiferous tubules histopathology of mice (Mus musculus) caused Fusarium graminearumexposure. This research was done in April-May 2017 in Microbilogy Laboratory Faculty of Science and Technology, Animal Laboratory, and Microbilogy Laboratory Faculty of Veterinary Medicine UniversitasAirlangga. This research used 20 male mice (Mus musculus) aged 6 weeks with 18-30 gram body weight. The mice devided into four groups: P0 given 0,25 ml Sodium chloride without Fusarium graminearum exposure by oral; P1 given 0,25 ml Fusarium graminearum exposure with dilution 102 by oral; P2 given 0,25 ml Fusarium graminearum exposure with dilution 103 by oral; and P3 given 0,25 ml Fusarium graminearum exposure with dilution 104 by oral. This treatment carried out for 21 days. Each milliliter dilution containing 228x106 spore for P1, 228x107 spore for P2, and 228x108 spore for P3. Then do the surgery and harvesting the testes then performed histopathological examination by scoring of seminiferous tubules. For data analyzing used non parametric difference Kruskall-Wallis and continued with Mann-Whitney. The result of this research was showed that decreased the spermatogenic cells in the process of spermatogenesis significant.
HUBUNGAN ANTARA BODY CONDITION SCORE (BCS) DENGAN PRODUKSI SUSU SAPI PERAH FRIESIAN HOLSTEIN (FH) Melsa Netika; Roesno Darsono; Budi Utomo; Imam Mustofa; Ismudiono Ismudiono; Tri Wahyu Suprayogi
Ovozoa : Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (332.995 KB) | DOI: 10.20473/ovz.v8i2.2019.89-93

Abstract

The aim of this research was to observe the Body Condition Score (BCS) and milk production of Friesian Holstein (FH) dairy cattles. 144 of dairy cattles divided into 3 groups based on BCS 1-9 scale. The results showed that Y = 6.62 + 5.54X - 5.78X2. Coefficient of determination (R2) of 20.6 this mean any increase in milk production by 20.6% is affected by BCS. It can be concluded that there is a correlation between Body Condition Score (BCS) and milk production. The higher BCS (BCS 7-8) the lower milk production, while in ideal BCS production (5-6) the production of milk is optimize.
PENGARUH pH LENDIR MUKOSA VAGINA SAAT BIRAHI TERHADAP PERSENTASE KEBUNTINGAN (Conception Rate) PADA SAPI PERAH DI KUD TANI WILIS KABUPATEN TULUNGAGUNG DAN KSU TUNAS SETIA BARU KABUPATEN PASURUAN Andaru Rizki; Pudji Srianto; Endang Suprihati; Trilas Sardjito; Ismudiono Ismudiono; Mohammad Anam Al Arif
Ovozoa : Journal of Animal Reproduction Vol. 8 No. 2 (2019): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (325.312 KB) | DOI: 10.20473/ovz.v8i2.2019.154-158

Abstract

The purpose of this research is to determine the effect of mucos acidity of vaginal mucous when estrus towards conception rate at KUD Tani Wilis Tulungagung District and KSU Tunas Setia Baru Pasuruan District. Measured samples were taken from 30 dairy cattles in each KUD. Mucos acidity of vaginal mucous were obtained by using a digital acidity meter before insemination was done on dairy cattle. After measuring the acidity from mucosal vagina mucous, artificial insemination has done on estrus dairy cattle. On the 60th day after insemination, rectal palpation was done to check the conception rate. Research result from 30 dairy cattle on each KUD showed that mucos acidity of vaginal mucous has signifficant effect towards conception rate. Mucos acidity of vaginal mucous range 6.5-8.5 with the highest range of conception rate in KSU Tunas Setia Baru Pasuruan District 7.5-7.7 and in KUD Tani Wilis Tulungagung District with the highest range of conception range 7.2-7.4 and 7.5-7.7.
Hubungan antara skor hasil bacaan heat detector dengan gambaran sitologi vagina sebagai parameter penentuan estrus pada kambing Nanda Irsa Meydilasari; Tatik Hernawati; Tita Damayanti Lestari; Erma Safitri; Trilas Sardjito; Ismudiono Ismudiono; Sri Hidanah
Ovozoa : Journal of Animal Reproduction Vol. 9 No. 1 (2020): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (655.361 KB) | DOI: 10.20473/ovz.v9i1.2020.7-11

Abstract

This study aimed to determine the relationship between heat detector reading results and the feature of vaginal swab preparations as a parameter for determining estrus in Peranakan Etawa goats. Five healthy non-pregnant goats weighing 20-30 kg, were synchronized using PGF2α (7.5 mg Dinoprost, Enzaprost) intramuscularly twice with 11 days interval. Vaginal cytology examination began the day after the second PGF2α injection. The heat detector (Draminski®, Dramiński SA, Poland) is an estrus detecting device based on the amount of electric current flowing through the vaginal mucus. Spearman correlation analysis showed a relationship (p <0.05) between heat detector reading and estrus detection based on vaginal cytology. The heat detector readings were very significantly correlated positively (p <0.01) with the number of epithelial cells (coefficient correlation, r= 0.564), but negatively correlated with the number of cornified cells (r = –0.854), and also significantly correlated positively (p <0.05) to the number of leukocytes (r = 0.487). The estrus stages were significantly correlated positively (p <0.05) only to the number of leukocytes (r = 0.918). Likewise, the number of epithelial cells was highly correlated negatively (p <0.01) only to cornified cells (r = –0,643), and the number of cornified cells was significantly correlated negatively (p <0.05) only to the number of leukocytes (r = –0.472). It could be concluded that the features of the proestrus, estrus and metestrus stages based on the goat vaginal cytology was correlated with the reading results of the heat detector.
Interkorelasi antara persentase konsentrat, kadar urea nitrogen susu dan beberapa variabel sapi perah Friesian Holstein Syaiful Rizal; Suzanita Utama; Budi Utomo; Suherni Susilowati; Sri Mulyati; Ismudiono Ismudiono; Soeharsono Soeharsono
Ovozoa : Journal of Animal Reproduction Vol. 9 No. 2 (2020): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v9i2.2020.53-59

Abstract

The purpose of this study was to identify the correlation among the percentage of feed concentrate, milk urea nitrogen (MUN), milk production, services per conception (S/C), calving interval (CI), and days open (DO) of Holstein Friesian cows on smallholder farms. Twenty four, 4-6 year old, non-pregnant, lactating cows, with body condition score (BCS) 5-7, having given birth 2-5 times were recorded their daily milk production, S/C, CI, DO, and the percentage of concentrate in feed given. Milk samples were collected to measure MUN. The correlation calculation among the six variables resulted 15 correlation values, ten of which were not significantly different (p >0.05), including the correlation between the percentage of concentrate and milk production on MUN. The other five correlation values were significantly different (p <0.05). The increase in concentrate intake was moderately correlated (r = 0.52), affecting 27.04% of the increase in milk production, and weakly correlated (r = 0.39), affecting 15.21% of the increase in S/C. The increase in milk production had a very strong correlation (r = 0.82), affecting 67.24% of the increase in S/C. The increase in S/C had a strong correlation (r = 0.61), affecting 37.21% of the increase in CI. The increase in CI was also affected 38.44% by the increase in milk production with a strong correlation (r = 0.62). It could be concluded that dairy cows with higher feed concentrate intake would be followed by an increase in milk production, but had a negative effect on reproduction in the form of increased S/C and CI.
Relationship between the value of the estrous detector measurement result and serum progesterone level in Etawa crossbred goats after estrous synchronization Annisa Lita Triagil; Ismudiono Ismudiono; Tita Damayanti Lestari; Abdul Samik; Budi Utomo; Boedi Setiawan
Ovozoa : Journal of Animal Reproduction Vol. 9 No. 3 (2020): Ovozoa : Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v9i3.2020.82-85

Abstract

This study was conducted to determine the relationship between the estrous detector's reading value (Draminski®, Dramiński S.A, Poland) and serum progesterone levels on Etawa crossbred does. Eighteen healthynon-prehnant Etawa crossbreds does weighing 20-25 kg, in their first parity were used in this study. The does were divided equally into two groups. In the two groups, the does were synchronized their estrus by means of intramuscular and intravulvar injection of 7.5 mg prostaglandin Fɑ (PGF2ɑ) twice in 11-day intervals. After the second injection, the estrous detector value measurement, visual observation of the estrus sign, and blood sampling were conducted for five days. The blood sample was processed into the serum to examine for progesterone levels by ELISA. The data analysis was using the simple correlation method on SPSS type 23 for windows. The result showed that a positive relationship (p <0.05) with coefficient correlation (r) was 0.609 between the values of the estrous detector measurement with serum progesterone levels. It could be concluded that the estrous detector's low value indicates that the Etawa crossbred was in the estrus phase and followed by the decreasing of progesterone level but not reaching the basal level.