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Analisis Klorofil Planlet (Spathoglottis plicata) Bl Plantlet Terhadap (Fusarium oxysporum) Gardis Andari; Endang Nurcahyani; Adrianus Adrianus
BioWallacea : Jurnal Penelitian Biologi (Journal of Biological Research) Vol 8, No 2 (2021): Biodiversitas Wallacea
Publisher : University of Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1207.172 KB)

Abstract

Orchids are ornamental plants that are in great demand by Indonesians, their prices are also very high. There are disturbances that occur in the growth of orchids, namely root rot and leaf spot. Fusarium wilt disease caused by Fusarium oxysporum is an important disease because it becomes an obstacle in the quality and production of orchids. The death rate of orchids in the United States caused by Fusarium oxysporum reaches more than 50% of the total number of orchids and this disease is difficult to control using fungicides. One of the efforts to control fusarium wilt disease safely, effectively, efficiently, and without negative impacts is by using high yielding varieties through tissue culture. Tissue culture applications using fusaric acid (AF) are widely used for in vitro selection of many plants. Plants infected with impacting materials will provide a resistance response (Induced resistance) and function to maintain plant survival, especially in warding off the attack of dangerous pathogens. The aim of this study is the specific expression character ofplantlets Spathoglottis plicata resulting from induced resistance to F. oxysporum based on total chlorophyll content, chlorophyll a chlorophyll b. The research method used tissue culture. The overall results of the study on chlorophyll a, clrophil b and total chlorophyll increased and were significantly different. In the control, the chlorophyll a content was 1.722 ± 1.1309E-02, chlorophyll b was 0.585 ± 2.5537E-02 and total chlorophyll was 2.309 ± 2.7503E-02. The chlorophyll a content in the 10 ppm treatment was 2.157 ± 4.7393E-02, the chlorophyll b was 1.470 ± 1.3237E-02, and the total chlorophyll was 3.625 ± 1.0504E-01. The chlorophyll a, b and total content increased in the 20 ppm treatment, namely chlorophyll a 2.834 ± 5.8198E-03, chlorophyll b 2.582 ± 1.8300E-03, total chlorophyll 5.413 ± 8.1599E-03. Chlorophyll a, b and total chlorophyll increased in 30 ppm treatment, namely chlorophyll a 3.297 ± 2.9527E-04, chlorophyll b 3,966 ± 9.1400E-03 and total chlorophyll of 7,258 ± 6.1367E-03. In the 40 ppm treatment, the content of chlorophyll a, b and total chlorophyll increased significantly, namely in chlorophyll a 3.957 ± 1.8898E-02, chlorophyll b 5.642 ± 3.6749E-01 and total chlorophyll of 9.592 ± 2.2269E-01 so it can be concluded that Chlorophyll a, b, and total chlorophyll content in ground orchid plantlet leaves that were resistant to Fusarium oxysporum increased compared to controls with higher concentrations of fusaric acid given ABSTRAK Anggrek merupakan tanaman hias yang banyak diminati oleh masyarakat indonesia, harganya juga sangat tinggi. Terdapat gangguan yang terjadi pada pertumbuhan tanaman anggrek, yaitu penyakit busuk akar dan bercak daun. Penyakit layu fusarium disebabkan Fusarium oxysporum merupakan penyakit penting karena menjadi salah satu kendala dalam kualitas dan produksi tanaman anggrek. Tingkat kematian tanaman anggrek di Amerika Serikat yang disebabkan oleh Fusarium oxysporum mencapai lebih dari 50% dari jumlah tanaman anggrek dan penyakit ini sulit dikendalikan dengan menggunakan fungisida. Salah satu usaha untuk mengendalikan penyakit layu fusarium dengan aman, efektif, efisien, tidak menimbulkan dampak negatif yaitu dengan menggunakan varietas unggul melalui kultur jaringan. Aplikasi kultur jaringan menggunakan Asam fusarat (AF) banyak digunakan untuk sleksi in vitro pada banyak tanaman. Tanaman yang diinfeksi bahan pengimbas akan memberikan respon ketahanan (Induced resistance) dan berfungsi untuk mempertahankan kelangsungan hidup tanaman, khususnya dalam menangkal serangan patogen yang berbahaya  Tujuan penelitian ini adalah Karakter ekspresi spesifik planlet Spathoglottis plicata hasil induce resistance terhadap F. oxysporum berdasarkan kandungan klorofil total, klorofil a klorofil b. Metode penelitian menggunakan kultur jaringan. Hasil penelitian secara keseluruhan pada klorofil a, klrofil b dan klorofil total mengalami peningkatan dan berbeda nyata. Pada kontrol  kandungan klorofil a yaitu 1,722 ± 1.1309E-02, klorofil b 0,585 ± 2.5537E-02  dan klorofil total 2,309 ± 2.7503E-02. Kandungan klorofil a pada perlakuan10 ppm yaitu 2,157 ± 4.7393E-02 , pada klorofil b 1,470 ± 1.3237E-02, dan pada klorofil total yaitu 3,625 ± 1.0504E-01. Kandungan klorofil a, b dan total mengalami peningkatan pada perlakuan 20 ppm yaitu klorofil a 2,834 ± 5.8198E-03 , klorofil b 2,582 ± 1.8300E-03 , klorofil total 5,413 ± 8.1599E-03. Pada klorofil a, b dan klorofil total mengalami peningkatan pada perlakuan 30 ppm yaitu pada klorofil a 3,297 ± 2.9527E-04, klorofil b 3,966 ± 9.1400E-03 dan klorofil total yaitu 7,258 ± 6.1367E-03. Pada perlakuan 40 ppm, kandungan klorofil a,b dan klorofil total mengalami peningkatan yang berbeda nyata yaitu pada klorofil a 3,957 ± 1.8898E-02 , klorofil b 5,642 ± 3.6749E-01dan klorofil total yaitu 9,592 ± 2.2269E-01  sehingga dapat disimpulkan bahwa kandungan klorofil a, b, dan klorofil total pada daun planlet anggrek tanah yang tahan terhadap Fusarium oxysporum mengalami peningkatan dibandingkan kontrol dengan semakin tinggi konsentrasi asam fusarat yang diberikanKey words: in vitro, klorofil, Spathoglottis plicata
PERTUMBUHAN DAN HASIL TIGA VARIETAS UBI JALAR (Ipomoea batatas L.) PADA TINGGI PETAKAN YANG BERBEDA Adrianus Adrianus
AGRICOLA Vol 2 No 1 (2012): AGRICOLA
Publisher : Universitas Musamus, Merauke, Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35724/ag.v2i1.104

Abstract

This study aim of the research is to find  the  growth and production of three varieties of sweet potato in different heights of maps. The resulted conducted in the experimental farm of Faculty of Agriculture,  Hasanuddin University in Makassar from March to July 2011.  The experiment was prepared using the design plots split consisted of 15 cm high, 30 cm and 45 cm high and three varieties of sweet potato as a sub plot, consisting of Japanese variety, Power variety and Purple/Poiret variety.  The results of the research reveal that  Japanese variety produces an average of the highest number of leaves (125.78 strands), the largest number of branches (6.19 strands), the heaviest leaves (164.00 g),  the highest weight of tubers per hectare (19.96 tons / ha ) and highest weight per tuber (171.73 g) The Power of varieties produced on average the heaviest stalk (295.75 g), the heaviest weight of tuber per plant (539.35 g), the heaviest   economical tuber weight per plant  (473.03 g), heaviest biomass (969.44 g), the longest shoots (451.18 cm) and the highest number of tubers (4.77 pieces). The plot with 30 cm high (p2) produces an average heaviest leaves (146.70 g), while the plot 45 cm (p3),  product  an average  longest tubers (14.57 cm).
Pengaruh Pemberian Jenis dan Dosis Pupuk Organik Cair Terhadap Pertumbuhan dan Produksi Tanaman Cabai Merah (Capsicum annum L.) Wahyuni Wahyuni; Adrianus Adrianus; Amelia A Limbongan
AGRICOLA Vol 8 No 2 (2018): AGRICOLA
Publisher : Universitas Musamus, Merauke, Papua

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Abstract

The research was aims to determine the effect of giving types and doses of organic liquid fertilizer on red chili plants. The use of the right type and dosage of fertilizer will affect the production of red chili plants better. The method used in this study used a factorial Randomized Block Design (FRBD) with the treatment of 2 types of fertilizers and 3 different doses of fertilizer then combined, so that there were 6 test treatments. The study was conducted 3 times, so we got 18 units of experimental design. The results showed that the interaction between treatment types and doses of organic liquid fertilizer did not have a real effect on all variables observed for growth components such as plant height and number of branches, but had a significant effect on the observed variables of production components, namely the total sample of fruit plants, sample crop production, and production per plot, with the type treatment and fertilizer dosage of 10 ml / liter of water.
Pemanfaatan Rizobakteri Penghasil Acetoin Sebagai Biofertilizer Untuk Memacu Pertumbuhan dan Hasil Tanaman Padi (Oryza Sativa L.) adrianus aris; gardis andari
AGRICOLA Vol 10 No 1 (2020): AGRICOLA
Publisher : Universitas Musamus, Merauke, Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35724/ag.v10i1.2750

Abstract

This study aims to look at the application of acetoin-producing rizobacteria as biofertilizers for boosting the growth of rice plants in dissolving phosphates and fixing nitrogen. The experimental design used was a randomized block design (RBD), consisting of 6 (six) treatments, 4 (four) treatments with rizobacteria isolates (Rg21 isolates, Pd13 isolates, Pd7 isolates, and Bb7 isolates), one treatment with synthetic acetoin, and one control. Each treatment was repeated 4 (four) times so that there were 24 experimental units consisting of 10 pots of rice plants. The size of the pot used is a surface diameter of 30 cm, filled with growth media in the form of a layer of paddy soil (taken to a depth of 20 cm from the soil surface) and compost with a ratio of 3: 1. The results showed that the treatment of rhizobacteria producing acetoin was able to spur the growth of rice plants by dissolving phosphates that were still bound by colloidal soil so that it was not available for plants to become available P. Likewise, rhizobacterial treatment is able to fix Nitrogen by changing enzymatically organic N compounds into ammonium
TEST APPLICATION OF LIQUID ORGANIC FERTILIZER DOSE FROM AGRICULTURAL WASTE ON CUCUMBER PLANTS (Cucumis Sativus L) Rangga Kusumah; Adrianus Adrianus; Amelia A. Limbongan
Musamus Journal of Agrotechnology Research Vol 1 No 1 (2018): MJAR
Publisher : Musamus University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (361.553 KB) | DOI: 10.35724/mjar.v1i1.1712

Abstract

The efficiency of fertilizer use is now a necessity in the management of farming in Indonesia. farmers dependents on anorganic fertilizer in the long term will results in soil and ecological damage. This research aims to find the efectivity of liquid organic fertilizer in different aplication doses on growth and production of cucumber plants. This study was conducted in Kampung Yasa Mulya, district of slope of Merauke regency. The design used in the study was a randomized design of a group with five treatments and three repeats. On the vegetative observation the treatment effect the secondary branch, but had no effect on the length of the main branch. In generative parameters, the treatment showed significant effect on the amount of potential flower, fruit diameter and fruit weight. The best results are demonstrated by D3 treatment on of the number of secondary branch with an average of 3.93 branch, and D4 treatment on observations of potential flowers rates with an average of 6.73 flower, the number of harvest fruits 16, the fruit length with an average of 16.63 cm, The diameter of the fruit with an average of 3.6 cm and on the observation of the fruit weights with an average of 296,5 g. Keywords: Liquid organic fertilizer; growth; cucumber
Pengaruh Pupuk Gandasil D Terhadap Pertumbuhan dan Produksi Kacang Tanah (Arachis hypogaea L.) Deni Soviani; Adrianus Adrianus; Abdullah Sarijan
Musamus Journal of Agrotechnology Research Vol 1 No 2 (2019): MJAR
Publisher : Musamus University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (158.49 KB) | DOI: 10.35724/mjar.v1i2.1850

Abstract

This study aims to determine the effect of Gandasil D fertilizer on growth and the production of peanut (Arachis hypogaea L.). The research was carried out in Kuprik, District Semangga, start from July until October 2012. The experimental design used was randomized block design with 4 (four) treatment and three replication. The treatment was Gandasil D fertilizer: 1,0 g/l, 1,5 g/l, 2,0 g/l and without Gandasil D fertilizer (control). The results showed that the treatment of Gandasil D fertilizer not showing significant effect on the growth and the production of peanut. But the treatment of D2 (Gandasil D) with dosis 1,5 g/l can increase the production a peanut higher than another treatment, the result is 1,40 kg/plot or 2,91 ton/ha.
Kampung Semangga Jaya Sebagai Sentra Produksi Pakan Ternak Sapi Abdul Rizal; Fredy Fredy; Adrianus Adrianus; Ineke Nursih Widyantari
Prima Abdika : Jurnal Pengabdian Masyarakat Vol. 1 No. 4 (2021): Volume 1 Nomor 4 Tahun 2021
Publisher : Program Studi Pendidikan Guru Sekolah Dasar Universitas Flores Ende

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37478/abdika.v1i4.1305

Abstract

Semangga Jaya Village, Merauke Regency as one of the villages that has high rice productivity. In 2019 rice production was 55,184.34 tons with a harvested area of 9,614 hectares. This figure is a potential benefit for the community. However, the high production of rice is not followed by the interest of farmers in utilizing rice straw waste to be processed into products that have high economic value, namely into complete feed that can be used as cattle feed. Cattle farming business is currently starting to be developed by farmers, although in practice it has not been fully maximized. This is due to the lack of public understanding of cattle development and the feeding system used is still simple or uses rice straw which basically has a high crude fiber content. The implementation of this activity will provide full participation in solving problems faced by cattle farmer groups, especially the feed processing system by utilizing rice straw waste into complete feed. Semangga Jaya Village, Merauke Regency in the future can be used as a center for cattle feed production. The implementation of activities includes counseling and technical guidance as a medium for transferring knowledge and technology to partners, which will be carried out en masse, through groups and individuals while still implementing Covid-19 health protocols. The solutions offered in the implementation of the program are through non-physical and physical activities. It is hoped that (a) Increased knowledge and skills of farmers regarding the system for processing rice straw waste into complete feed, (b) Increased knowledge and skills in feeding cattle by looking at feed quality standards, (c) Increased knowledge and ability of farmers and breeders to produce feed into attractive packaging for sale through offline and online systems.
Karakteristik Usaha Ternak Sapi Terintegrasi Dengan Tanaman Padi Di Distrik Semangga Kabupaten Merauke Nurliah Nurliah; Adrianus Adrianus; Ineke Nursih Widyantari; Abdul Rizal
Musamus Journal of Livestock Science Vol 5 No 1 (2022): Musamus Journal of Livestock Science
Publisher : Faculty of Agriculture, Musamus University

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Abstract

This research is a qualitative research which describes the condition of farmers and ranchers in the research location based on the observed variables. The method used is a survey method, with the sampling technique is Stratified Random Sampling. This location was chosen because it is one of the districts where the majority of the people are farmers and breeders with an integrated system of rice plants and cattle, while the population in this study is the people of the Semangga District who carry out integrated farming of rice and cattle raising. Sources of data in this study are primary data and secondary data. Data collection was carried out using several techniques, namely (a) Observation, namely direct observation in the field (b) Interview, conducted directly to farmers and ranchers with the help of questionnaires and (c) Documentation, namely taking pictures in the field. The data analysis used is descriptive analysis by looking at the average and percentage on each business characteristic. The conclusion of this study is that the average number of cattle kept is 2 heads with a land area of 3 ha, the highest percentage of education level is SD (49%) and the lowest is SI (1%), the average age of the respondents is in the productive age, namely 26-50 years (94%), the highest length of business is 6-10 years (53%) and the average family responsibilities are 1-4 people (94%).
Pengaruh Pemberian Dosis Pupuk Organik Cair Terhadap Pertumbuhan dan Produksi Tanaman Jagung Manis (Zea mays saccarata Sturt) Arief Rohman Faruq; Adrianus Adrianus adrianus; Amelia Limbongan
Musamus Journal of Agrotechnology Research Vol 3 No 1 (2020): MJAR
Publisher : Musamus University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35724/mjar.v3i1.4244

Abstract

This research aims to determine the best dose of Papuagro liquid organic fertilizer on the growthand production of sweet corn (Zea Mays Saccarata Sturt) in Yasa Mulya Village, Tanah Miring,Merauke Regency. This study use a randomized block design experiment, with 5 treatments and3 replications. This research shows that the combination treatment of dosing of Papuagro liquidorganic fertilizer gives the significant influence at the level of 0.05% on cob’s diameter and thetotal weight per plot. While at the level of 0.01% gives a significant influence on the number ofseeds per line, cob’s weights per plant samples. In some of these combination of liquid organicfertilizer dose treatments are able to give a good results. The treatment of Papuagro liquid organicfertilizer dosing using the D3 combination (140ml and 90ml/ 15 L water) gives the best result insome observation than other treatment.Keywords: Sweet Corn; Growth; Production; Organic Liquid Fertilizer Dose.
PEMBIAKAN BIBIT F2 JAMUR TIRAM (Pleurotus ostreatus) PADA BERBAGAI DOSIS DEDAK SECARA INVITRO MENGGUNAKAN MEDIA TANAM AMPAS SAGU Sania dwintami; Adrianus Adrianus; Andri Prasetia; rosmala widijastuti
Musamus Journal of Agrotechnology Research Vol 4 No 1 (2021): MJAR
Publisher : Musamus University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35724/mjar.v4i1.4573

Abstract

Absract Sago palm waste is a potential media for oyster mushroom cultivation because of cellulose and lignin content,yet it still requires supplementary materials to improve the media,s nutritional status. This research aims to assess the effect of sago palm media supplemented with varying research was designed using a completely randomized design with one treatment factor consisting of four treatments (0g, 20g, and 30g) with 20 replications. The research result revealed that sago palm waste media supplementing with 30g of rice-milled waste is the best combination for mycelium growth in oyster mushrooms (Pleurotus ostreatus) F2 nursery. Keywords: Mushroom, Sago Palm Waste, Sago Palm Waste, Mycelium