Sunarno Sunarno
Biology Laboratory Structure And Function Of Animals, Department Of Biology, Faculty Of Science And Mathematics, Diponegoro University, Jl. Prof. Soedarto, SH, Tembalang, Semarang

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Journal : Jurnal Biologi Tropika

Aplikasi biomaterial aktif dari daging ikan gabus (Channa striata) untuk penyembuhan luka pascaoperasi pada hewan model tikus Wistar Sunarno Sunarno; Rahayu Damayanti; Alfisa Devi; Muhammad Fikri; F Pratiwi; Linda Ayu
Jurnal Biologi Tropika Vol.1, No. 2, Tahun 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (532.757 KB) | DOI: 10.14710/jbt.1.2.13-20

Abstract

One problem for postoperative patients is tissue recovery takes a long time due to the occurrence of blood clotting disorders and immune system. Snakehead fish was known to contain bioactive ingredients that can potentially speed up the process of tissue repair as a result of injuries. Snakehead fish meat contains a lot of minerals, albumin, and amino acids needed for tissue protein synthesis and increases the antioxidant glutathione. This study was conducted to test the extraction of snakehead fish flesh against skin tissue wound healing in Wistar rats. The study was conducted with a completely randomized design consisting of three treatments with 6 replication, which include (P0) a negative control (without treatment madecassol and snakehead fish meat extract), (P1) madecassol commercial drug treatment, and (P2) treatment of snakehead fish meat extract. Each material was injected intramuscularly in Wistar rats at a dose of  9 ml/kg bb for 7 days. Data analysis uses Anova followed by test Honestly Significant Difference (HSD) with a significance of 95%. Treatment of snakehead meat extract with a dose of 9 ml/kg bb a significant effect on skin tissue repair after injury, better than treatment with madecassol and control with the average value of extensive tissue remaining after recovering from the condition of the wound by 0.2 cm2; 0.33 cm2; and 0.4 cm2, respectively. Snakehead fish meat extract with a dose of 9 ml/kg bb injected intramuscularly can accelerate the recovery of tissue in the skin after the wound in Wistar rats. 
Bobot otot dan tulang tibia itik pengging (Anas platyrhyncos domesticus L.) setelah pemberian imbuhan tepung daun kelor (Moringa oleifera Lam.) dalam pakan Hikmah Putri Pratiwi; Kasiyati Kasiyati; Sunarno Sunarno; Muhammad Anwar Djaelani
Jurnal Biologi Tropika Vol. 2, No. 2, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (207.952 KB) | DOI: 10.14710/jbt.2.2.54-61

Abstract

Moringa leaf contains the nutrient which includes carbohydrates, protein, fat, fiber, beta-N, calcium, and phosphor. Various kinds of nutrient are essential supporting the growth and productivity of Pengging ducks, both egg or meat production. The aimed of this study to analyze the effect of Moringa leaves powder in feed on the weight of tibial bone and muscle as well as the length and diameter of tibial bone in Pengging ducks. The study used a Completely Randomized Design (CRD) consisting of 5 treatments with 3 replications. The treatment included the control group (basal feed without the addition of Moringa leaves powder), the treatment of basal feed supplemented with Moringa leaves powder by 2.5; 5; 7.5; and 10%. The data wereanalyzed by one way-ANOVA and the relationship between variables can be seen from the value of the correlation coefficient. The results showed that the added Moringa leaves powder did not significantly (P > 0.05) affect the weight of the tibial bone and muscle, and the lenght and diameter of the tibial of the pengging duck. The positive correlation between the length of thetibial bone and the diameter of the tibial bone with an r-value by 0.894. It was concluded that Moringa leaves powder as feed additive of Pengging duck could not increase the weight of the tibial bone and muscle, and the length and diameter of the tibial bone had not changed.
Suplemen kombucha dan kayu manis untuk menghasilkan daging broiler kaya antioksidan dan rendah kolesterol Laili Fitria Zulfa; Sunarno Sunarno; Salsabila Alifah; Salindri Prawitasari
Jurnal Biologi Tropika Vol. 2, No. 1, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (763.503 KB) | DOI: 10.14710/jbt.2.1.34-40

Abstract

Quality and healthy meat is the goal of broiler cultivation. One way to get quality, safe broiler meat is by giving kombucha tea and cinnamon flour supplements in a diet that can lower cholesterol and increase the antioxidant content of meat. This study aims to analyze the cholesterol and antioxidant content of broiler (femoris and  pectoralis major muscles) after the supplementation of kombucha tea and or cinnamon flour in feed. This study uses a completely randomized design consisting of 5 treatments with      4 replications. The treatment in this study included P0: control, without being given kombucha tea and cinnamon flour, P1: 5% cinnamon flour; P2, P3, and P4 respectively are 100% kombucha tea and 5% cinnamon flour with a ratio (3: 1), (1: 1), and (1: 3). The variables measured were total antioxidant and cholesterol levels in the femoral and pectoral major muscles. The research data were analyzed by ANOVA followed by Duncan Test with a confidence level of 95%. The results showed that kombucha tea and cinnamon flour supplements significantly affected antioxidant levels and total cholesterol in broiler meat. The conclusion of this study is that 100% kombucha tea with 5% cinnamon with a ratio (3: 1) and (1: 1) gives the most optimal influence on increasing antioxidant levels and decreasing cholesterol levels in femoral muscle and major pectotalis in broilers.
Pengaruh imbuhan tepung daun kelor (Moringa oleifera Lam.) dalam pakan pada kandungan protein dan kolesterol telur itik pengging (Anas platyrhyncos domesticus L.) Khunfaya Firnanda Putri Rossida; Sunarno Sunarno; Kasiyati Kasiyati; Muhammad Anwar Djaelani
Jurnal Biologi Tropika Vol. 2, No. 2, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (210.522 KB) | DOI: 10.14710/jbt.2.2.41-47

Abstract

One important component contained in Moringa leaves is protein. Apart from protein, bioactive flavonoids and saponins in Moringa leaves have potential as antioxidants and hypocholesterolemia. The objective of this study was to analyze the effect of Moringa leaf flour as a feed additive to protein content and cholesterol of Pengging duck eggs. The study design used was a Completely Randomized Design (CRD) with 5 treatments, and each treatment consists of 3 replications. The treatment group consisted of a control group and a basal feed treatment that was added with Moringa leaf flour by 2.5; 5; 7.5; and 10%. Measurement of duck egg protein content using the Bradford method and cholesterol measured by the Indiko method. Data were analyzed with one-way ANOVA. The addition of Moringa leaf flour on feed did not have a significant effect (P > 0.05) on the protein content and cholesterol of the Pengging duck eggs. The percentage of white and egg yolk weights did not increase significantly. The conclusion of this study was the use of Moringa leaf flour as a feed additive with a concentration of 2.5 to10% could not change the protein content and cholesterol of the Pengging duck eggs.
Aplikasi larutan EMOVA daun kelor (Moringa oleifera) dan daun afrika (Vernonia amygdalina) dalam menekan mortalitas ikan nila (Oreochromis niloticus) pada simulasi transportasi Sunarno Sunarno; Eko Purnomo; Khunfaya Firnanda Putri Rossida; Maulida Aqlinia; Falasifah Falasifah; Mamluatul Lailiyah
Jurnal Biologi Tropika Vol. 2, No. 1, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (185.1 KB) | DOI: 10.14710/jbt.2.1.8-15

Abstract

Fish transportationis logistical aspect of the fishery business. Wrong procedures in handling fish during transportation can trigger stress. Herbal ingredients such as kelor (Molinga oleifera) and afrika leaf (Vernonia amygdalina) have the potential to overcome stress on fish. Both types of herbal ingredients contain compounds of flavonoids and phenols that have the potential to be analgesik. The purpose of this study was to analyze the analgesik effect of kelor and afrika leaf extract to nila fish (Oreochromis niloticus) using transportation simulation model. The experimental design was usedcomplete randomized design with four treatments, including the cold temperature of 10ºC (L0), the extract solution of kelor and afrika leaf was use ratio 1:1 with concentrations of 10% (L1), 20% (L2) and 30% (L3) solution. The main parameters observed were viability and mortality. The experiment results showed that the treatment of L1 and L2 was not significantly different with the control, L2 treatment was not significantly different with L3 but L3treatment was significantly different with kontrol and L1. The viability of the L3 treatment is higher than control and treatment of L1, as well as for mortality parameters. The conclusion of this research is, extract of kelor and afrika leaf combination with 1:1 ratio at 30% concentration in transportation simulation model can cause high viability value and able to depress mortality. The results of this study can be used as a solution to fish mortality due to stress and handling of fish based on animal welfare principle during transportation.
Bobot telur, indeks bentuk telur, dan nilai kantung udara telur itik pengging setelah pemberian imbuhan tepung daun kelor (Moringa oleifera Lam.) dalam pakan Novia Marcelina; Muhammad Anwar Djaelani; Sunarno Sunarno; Kasiyati Kasiyati
Jurnal Biologi Tropika Vol. 3, No. 1, Tahun 2020
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (315.566 KB) | DOI: 10.14710/jbt.1.1.1-7

Abstract

Moringa oleifera leaves contain nutrition of protein, lipid, carbohydrate, fiber, calcium, phosphorus as well as bioactive content such as tannin and saponin, that have the potential to improve egg quality. The objective of this research to evaluate the effect of Moringa powder of weight, shape index, and air space value of Pengging duck eggs. The study used a completely randomized design (CRD) consisting of 5 treatments with four replication, i.e., K0 (100% basal feed without Moringa leaves powder), K1 (basal feed and 2.5% Moringa leaves powder), K2 (basal feed and Moringa leaves powder), K3 (basal feed and 7.5% Moringa leaves powder) and K4 (basal feed and 10% Moringa leaves powder). The data were obtained  from a total of 60 female Pengging duck of 28 weeks old or sexually mature. Feed treatments administrated for six weeks started in 26 to 32 weeks old ducks. The measurement of research variables was carried out every week and measured for three weeks. The data of the study were analyzed by one way-ANOVA at a significant difference level of  5%. The results showed that the addition of Moringa leaves powder did not a significant difference (P > 0.05) in weight, shape index, and air space value of pengging duck eggs. Overall concluded that the addition of Moringa leaves powder with a concentration of 2.5; 5; 7.5; dan 10% in Pengging duck feed could not change egg quality.
Bobot Karkas dan Bagian Karkas Ayam Petelur Jantan (Gallus gallus domesticus L.) Setelah Pemberian Tepung Daun Kelor (Moringa oleifera Lam.) Sebagai Imbuhan Pakan Dwi Rahmawati; Muhammad Anwar Djaelani; Kasiyati Kasiyati; Sunarno Sunarno
Jurnal Biologi Tropika Vol. 3, No. 2, Tahun 2020
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbt.3.2.65-72

Abstract

Consumption of meat as a source of animal protein has increased every year, especially the consumption of rooster. The increasing cost of poultry feed has made it difficult for breeders to produce roosters that have meat with high protein content. The cost of poultry feed can be minimized by the addition of herbal ingredients, one of which is the addition of leaf powder. Moringa oleifera leaf powder contains various kinds of amino acids and antioxidants that can be used as a supplement to additives feed. This study aimed to evaluate the effect of M. oleifera leaf powder on carcass weight rooster (Gallus gallus domesticus L.). Completely randomized design (CRD) used in this study consisted of 5 treatment groups with three replications, namely the control treatment group and the basal feed treatment group supplemented with Moringa leaf powder 1; 2; 3; and 4%. The feed used is the standard starter and finisher period rations formulated with Moringa leaf powder according to their respective concentrations. Parameters were measured during the seventh week when the rooster was 49 days old, which included: body weight, carcass weight, breast weight, and thigh weight. Based on the research conducted, it was found that giving Moringa leaf powder 1; 2; 3; and 4% as feed additives in rooster had no significant effect (p> 0.05) on body weight, carcass weight, breast weight, and thigh weight. Overall, the conclusion of this study is the addition of Moringa leaf powder with a concentration of 1; 2; 3; and 4% in rooster feed up to 7 weeks of age did not affect on carcass weight, breast weight, and thigh weight of rooster.