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All Journal Jurnal Biologi Udayana Bioma : Berkala Ilmiah Biologi BERKALA FISIKA JURNAL ANATOMI FISIOLOGI SAINS DAN MATEMATIKA Biogenesis: Jurnal Ilmiah Biologi AGRIVITA, Journal of Agricultural Science Kultivasi Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Biologi Tropika NICHE Journal of Tropical Biology Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Life Science
Nintya Setiari
Departemen Biologi, Fakultas Sains Dan Matematika Universitas Diponegoro
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Physics Public Health

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Perlakuan Defoliasi untuk Meningkatkan Pembentukan dan Pertumbuhan Cabang Lateral Jarak Pagar (Jatropha curcas) Darmanti, Sri; Setiari, Nintya; Romawati, Tanti Dwi
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 16, No 2 (2008): Vol. XVI, No. 2, Oktober 2008
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (41.762 KB) | DOI: 10.14710/baf.v16i2.2589

Abstract

Jatropha is an alternative resource of sustainable energi. Therefore, to support the use of sustainable energi,an increase production of Jatropha seed is important. As Jatropha seeds (fruits) only growth at terminal andaxial bud, therefore the presence of lateral bud is necessary to increase production of Jatropha seed.Formation of lateral bud is affected by the balance between auxin and sitokinin. Defoliation will control thebalance of these two hormone. In this study, defoliation is conducted on seedling at the age of 4 month.Each treatment was replicated    7 times. Variabel were observed and ended after 6 month. Result indicatecthat either defoliation will stimulated the formation and growth of lateral bud.
Pembentukan Akar pada Stek Batang Nilam (Pogostemon cablin Benth.) setelah direndam Iba (Indol Butyric Acid) pada Konsentrasi Berbeda Hasanah, Farida Nur; Setiari, Nintya
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 15, No 2 (2007): Volume XV, Nomor 2, Oktober 2007
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (37.831 KB) | DOI: 10.14710/baf.v15i2.2566

Abstract

This paper studies the effect of plant growth regulators, i.e., indol butyric acid (IBA), to the root growth in stem cutting technique of P. cablin, and the correct concentration of the IBA to obtain optimum root growth. The research were carried out in Lab Bio Struktur dan Fungsi Tumbuhan, UNDIP, Semarang. Complete Randome Design was used as the research method in which 4 different concentrations, i.e., control 0, 25, 50 and 75 ppm, were observed with parameter of the amount of the root, the length of the root, wetted weight, dried weight, and efficiency. The results show immersing the stem into 25 ppm IBA exhibits the highest root growth efficiency.
Respon Pertumbuhan dan Produksi Alkaloid pada Kalus Berakar Datura metel L. terhadap Peningkatan Mikronutrien dari Medium MS Hadayani, Wahyu; Nurchayati, Yulita; Setiari, Nintya
BULETIN ANATOMI DAN FISIOLOGI dh SELLULA Vol 12, No 1 (2012): VOL XX, NOMOR 1, TAHUN 2012
Publisher : BULETIN ANATOMI DAN FISIOLOGI dh SELLULA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (242.93 KB) | DOI: 10.14710/baf.v12i1.4763

Abstract

Senyawa alkaloid dari tanaman Solanaceae memiliki potensi obat antara lain sebagai antibakteri bahkan memberi efek halusinasi. Alkaloid pada kecubung, Datura metel disintesis pada organ akar dan diakumulasi pada bagian pucuk. Salah satu metoda untuk produksi senyawa alkaloid tersebut adalah dengan menginduksi perakaran dari kalus yang berasal dari daun (induksi kalus berakar). Produksi alkaloid dapat ditingkatkan dengan memodifikasi komponen mikronutrien dari medium dasar MS (Murashige&Skoog).  Penelitan ini bertujuan untuk mempelajari pengaruh peningkatan konsentrasi mikronutrien terhadap pertumbuhan dan kandungan alkaloid total dari kalus berakar.  Kultur diperoleh dari induksi kalus dalam medium MS tanpa zat tumbuh dengan perlakuan konsentrasi mikronutrien.  Pertumbuhan kultur ditentukan dari berat segar dan berat kering,   sedangkan kandungan alkaloid total dianalisis dengan metoda titrasi. Data dianalisis secara deskriptif. Hasil penelitian menunjukkan  bahwa peningkatan konsentrasi mikronutrien hingga 2,5 kali dari medium MS menghambat pertumbuhan kalus berakar. Namun demikian, kondisi ini tampak tidak mampu memacu produksi senyawa alkaloid dari kultur.
Respon Pertumbuhan dan Produksi Senyawa Antioksidan pada Kalus Hibiscus sabdariffa L. dari Eksplan yang Berbeda secara in vitro Noviati, Agustin; Nurchayati, Yulita; Setiari, Nintya
JURNAL SAINS DAN MATEMATIKA Volume 22 Issue 1 Year 2014
Publisher : JURNAL SAINS DAN MATEMATIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3638.594 KB)

Abstract

Ascorbic acid and carotenoid are secondary metabolites found in roselle, which show antioxidant activity. These compounds can be obtained from callus induced  by several kinds of explants. The aims of this experiment is to study callus growth from explants which can encourage high level of antioxidant compounds. The callus was obtained from difference organ, i.e. section of leaf, petiole and flower sepal. Sterilized explants were planted in MS (Murashige&Skoog) combined with 2 mg/L Naphtalene Acetic Acid (NAA) dan 5 mg/L Benzyl Adenin (BA).  This experiment has been conducted by completly randomized design with 5 replicates. Besides fresh weight callus, callus respons from each explants were analyzed descrptively.  Ascorbic acid and carotenoid content were analyzed qualitatively and quantitatively by titration and spectrophotometric respectively.  The results showed that all kinds of explant dedifferentiated into callus which antioxidant content. Callus from leaf section had the higgest fresh weight with high level ascorbic acid. Whereas the higgest carotenoid level was obtained from callus-derived flower sepals. It conclused that  in vitro callus was useful for producing plant biochemical compounds. Keywords : Callus induction, antioxidant agents, explants, ascorbic acid, cartenoid
PENGGUNAAN NAUNGAN DAN TIPE SUBSTRAT YANG BERBEDA PADA PERIODE AKLIMATISASI EX VITRO PHALAENOPSIS HIBRID Fauziah, Ade Arisma; Prahasti, Nurullita; Setiari, Nintya; Saptiningsih, Endang
Buletin Anatomi dan Fisiologi (Bulletin of Anatomy and Physiology) Volume 5, Nomor 1, Tahun 2020
Publisher : Departemen Biologi Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/baf.5.1.2020.%p

Abstract

Produksi bibit anggrek Phalaenopsis Hibrid secara umum dilakukan secara in vitro melalui teknik kultur jaringan. Periode selanjutnya adalah tahap aklimatisasi yaitu adaptasi plantlet di lingkungan ex vitro sehingga dapat tumbuh, bertahan hidup dan memproduksi bunga yang optimal. Penggunaan naungan dan tipe substrat berperan terhadap pertumbuhan dan kelangsungan hidup plantlet selama periode awal aklimatisasi. Penelitian bertujuan untuk mengkaji peran naungan dan tipe substrat yang berbeda terhadap pertumbuhan dan kelangsungan hidup plantlet Phalaenopsis Hibrid selama periode awal aklimatisasi ex vitro. Penelitian dilakukan secara eksperimental dalam lingkungan greenhouse menggunakan plantlet Phalaenopsis Hibrid dalam botol, naungan paranet dan perlakuan tipe substrat yaitu serabut kelapa, akar kadaka dan spagnum. Penelitian dilakukan selama 5 minggu di greenhouse Jurusan Biologi, Fakultas Sains dan Matemetika, UNDIP, Semarang. Plantlet tumbuh dan mampu bertahan hidup pada semua substrat dan naungan paranet secara bertahap. Pembentukan pori yang besar pada substrat serabut kelapa dan akar kadaka mampu meningkatkan jumlah dan panjang akar. Kemampuan menjerap air tinggi pada spagnum dapat meningkatkan berat segar plantlet. Penutupan paranet secara bertahap dan penggunaan substrat serabut kelapa, akar kadaka serta spagnum dapat mempertahankan kelangsungan hidup plantlet selama periode awal aklimatisasi ex vitro.   Kata kunci: aklimatisasi, anggrek, Phalaenopsis Hibrid, substrat, naungan
Pertumbuhan Kalus Tomat (Lycopersicon esculentum Mill.) Varietas Permata F1 dari Jenis Eksplan dan Konsentrasi Sukrosa yang Berbeda secara In Vitro Ulva, Maria; Nurchayati, Yulita; Prihastanti, Erma; Setiari, Nintya
Life Science Vol 8 No 2 (2019): November 2019
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v8i2.37103

Abstract

culture. Callus growth is influenced by the type of explant and the composition of the planting medium, one of which is sucrose concentration. The purpose of this study was to obtain the best type of explants for callus culture and find out the optimum sucrose concentration for callus growth. The method used is callus induction in Murashige and Skoog (MS) media, with the treatment of sucrose concentration and explant types. Explants were grown in MS media with the addition of 1 ppm naphthalene acetic acid (NAA) and 1 ppm benzyl amino purine (BAP). This study uses explants in the form of hypocotyl and cotyledons from tomato sprouts in vitro. The design of this study used a completely randomized design (CRD) in 2x4 factorial pattern. The first factor is the type of explants in the form of hypocotyl and cotyledons. The second factor is sucrose concentration which is 10, 20, 30 and 40g/L. The parameters observed were initiation time, wet weight, dry weight and callus morphology. The results showed that the treatment of explant type did not affect the growth of tomato callus, but different concentrations of sucrose in the media significantly affected. Sucrose at 30-40 g/L is a concentration that can stimulate the growth of tomato callus, both in hypocotyl explants and tomato cotyledo. Keywords: in vitro culture, hypocotyl, cotyledons, sucrose, callus, kultur in vitro, hipokotil, kotiledon, sukrosa, kalus. udidaya tanaman tomat (Lycopersicon esculentum Mill.) dapat dilakukan dengan cara kultur in vitro melalui kultur kalus. Pertumbuhan kalus dipengaruhi oleh jenis eksplan dan komposisi media tanam, salah satunya konsentrasi sukrosa. Tujuan penelitian ini untuk memperoleh jenis eksplan yang paling baik untuk kultur kalus dan mengetahui konsentrasi sukrosa yang optimum untuk pertumbuhan kalus. Metode yang digunakan adalah induksi kalus dalam media Murashige and Skoog (MS), dengan perlakuan konsentrasi sukrosa dan jenis eksplan. Eksplan ditumbuhkan dalam media MS dengan penambahan Naphthalene Acetic Acid (NAA) 1 ppm dan Benzyl Amino Purin (BAP) 1 ppm. Penelitian ini menggunakan eksplan berupa hipokotil dan kotiledon dari kecambah tomat secara in vitro. Desain penelitian ini menggunakan Rancangan Acak Lengkap (RAL) pola faktorial 2x4. Faktor pertama adalah jenis eksplan berupa hipokotil dan kotiledon. Faktor kedua adalah konsentrasi sukrosa yaitu 10, 20, 30 dan 40g/L. Parameter yang diamati yaitu waktu inisiasi, berat basah, berat kering, dan morfologi kalus. Hasil penelitian menunjukkan bahwa perlakuan jenis eksplan tidak berpengaruh terhadap pertumbuhan kalus tomat, namun konsentrasi sukrosa yang berbeda pada media berpengaruh secara signifikan. Sukrosa sebesar 30 - 40g/L merupakan konsentrasi yang dapat memacu pertumbuhan kalus tomat, baik pada eksplan hipokotil maupun kotiledon kecambah tomat. Kata kunci: in vitro culture, hypocotyl, cotyledons, sucrose, callus, kultur in vitro, hipokotil, kotiledon, sukrosa, kalus
Pertumbuhan Kecambah Kentang (Solanum tuberosum L.) secara In Vitro pada Konsentrasi NaClO dan Waktu Sterilisasi yang Berbeda Nida, Khoirin; Luaeliyah, Masrukhatul; Nurchayati, Yulita; Izzati, Munifatul; Setiari, Nintya
Life Science Vol 10 No 1 (2021): April 2021
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v10i1.47165

Abstract

Potato (Solanum tuberosum L.) have been cultivated with tubers. One alternative to the propagation of potatoes can be done with seeds that are germinated in vitro. The factors that influence the germination of potato seeds are the concentration of sterilant and time of sterilization. The purpose of this study was to determine the proper sterilant concentration and sterilization time to increase growth in the optimal percentage of germination and potato sprouts. The method used is in vitro culture with different concentrations and time treatment. This study used explants of potato seeds which were sterilized with a concentration of 15% and 20%, 1 and 3 minutes of sterilization. The design used a completely randomized design 2x2 factorial. The factor are sterilant concentration and sterilization time. The parameters observed were germination time, plant height, number of leaves and roots. The results showed that sterile concentration did not affect of germination time but affected the germination. A 15% concentration indicates that germination occurs rapidly, while a 20% concentration inhibits germination. A 15% concentration results in an optimal number of leaves and plant height. The higher the concentration of sterile, tends to inhibit the growth of roots and leaves. Keywords: Sprouts; potatoes; in vitro culture; NaClO; growth; sterilization, Kecambah; kentang; kultur in vitro; NaClO; pertumbuhan; sterilisasi Tanaman kentang (Solanum tuberosum L.) selama ini dibudidayakan dengan umbi. Salah satu alternatif perbanyakan kentang dapat dilakukan dengan biji yang dikecambahkan secara in vitro. Faktor yang mempengaruhi perkecambahan biji kentang adalah konsentrasi sterilan dan waktu sterilisasi. Tujuan penelitian ini adalah mengetahui konsentrasi sterilan (yang mengandung zat aktif 5,25% NaClO) dan waktu sterilisasi yang tepat untuk meningkatkan persentase perkecambahan dan pertumbuhan kecambah kentang. Metode yang digunakan adalah kultur in vitro biji kentang dengan perlakuan konsentrasi sterilan dan waktu sterilisasi yang berbeda. Penelitian ini menggunakan eksplan biji kentang yang dilakukan sterilisasi dengan konsentrasi sterilan 15% dan 20% dan waktu sterilisasi 1 menit dan 3 menit. Desain penelitian ini menggunakan Rancangan Acak Lengkap (RAL) pola faktorial 2x2. Faktor pertama adalah konsentrasi sterilan dan faktor kedua adalah waktu sterilisasi. Parameter yang diamati adalah waktu perkecambahan, tinggi tanaman, jumlah daun, dan jumlah akar. Hasil penelitian menunjukkan bahwa konsentrasi sterilan tidak berpengaruh terhadap waktu perkecambahan dan pertumbuhan kecambah. Konsentrasi sterilan 15% menyebabkan waktu perkecambahan cenderung lebih cepat dan konsentrasi sterilan 20% cenderung menghambat pertumbuhan kecambah. Keywords: Sprouts; potatoes; in vitro culture; NaClO; growth; sterilization, Kecambah; kentang; kultur in vitro; NaClO; pertumbuhan; sterilisasi
Glutamic Acid Application for Enhancement of Growth and Productivity of Okra Plant (Abelmoschus esculentus L. Moench) Eva Septiyana; Nintya Setiari; Sri Darmanti
Biogenesis: Jurnal Ilmiah Biologi Vol 7 No 2 (2019)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v7i2.10146

Abstract

Red okra fruit has high nutritional value and fiber but still has low production. Monosodium glutamate (MSG) consists of sodium and glutamic acid. In plants, sodium can role of replacing potassium, stomata physiology, and chlorophyll biosynthesis. Glutamate as the nitrogen donor in primer metabolism and gibberellic acid precursor. The assumption that MSG in plants has a positive impact. This research aimed to examine the effect of MSG and the optimum dosage for enhances of growth and production. This research was conducted with Completely Randomized Designs (CRD) with one factor, is the MSG dosage (0, 3, 6, and 9 g). Quantitative data were analyzed using analysis of variant (ANOVA) dan Duncan’s Multiple Range Test (DMRT) at a 95% confidence level. The result shows that the MSG treatment enhances the growth of the okra based on parameters of plant’s height, fresh weight of plant, dry weight of plant and the width of the leaf and enhances the production of the okra based on the flowering time, the number of the flower, the number of the fruit, the percentage of flower becoming fruit, the weight of fruit, width of fruit, and the diameter of the fruit. The optimal dosage of MSG for okra production and growth is 3 g/plant.
Pertumbuhan planlet anggrek Cymbidium bicolor Lindl. pada tahap subkultur dengan variasi media Fakhri Fadhlurrohman Pratama; Nintya Setiari; Yulita Nurchayati
Jurnal Biologi Udayana Vol 25 No 1 (2021): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2021.v25.i01.p08

Abstract

Cymbidium bicolor is one of the orchids in Indonesia which has beautiful and longlasting flowers, so it has potential to become an ornamental plant and has economic value. The method of propagation generally is tissue culture. One of the stages in plant tissue culture is subculture. The variation of media used at the subculture stage make an effect for growth of plantlet. Media usually can be used include Murashige and Skoog (MS), Vacin and Went (VW), and New Phalaenopsis (NP). This study aims is to analyze the variation of media during the subculture. The method used was tissue culture in subculture with media as treatment. Cymbidium bicolor planlets were subcultured into MS, VW, and NP and observed for 2 months. The parameters observed were the leaf emergence, the number of leafs, leaf length, leaf width, the number of roots, root length, and the number of shoots. This study used a completely randomized design with single factor and 15 replicates. Data were analyzed by ANOVA with 95% significance. Treatment of variation of media significantly affected all parameters. Murashige and Skoog (MS) medium was the best medium for leaf emergence (11 days), the number of leafs (4,92), leaf length (1,56 cm), leaf width (0,33 cm), the number of roots (2,67), root length (2,73 cm), and the number of shoots (4,00). Murashige and Skoog (MS) medium was the best medium and concentrations for C. bicolor growth at the subculture stage.
Kandungan klorofil dan karotenoid Kacang Tunggak (Vigna unguiculata (L.) Walp.) pada umur tanaman yang berbeda Ika Susanti Hendriyani; Yulita Nurchayati; Nintya Setiari
Jurnal Biologi Tropika Vol.1, No. 2, Tahun 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (584.952 KB) | DOI: 10.14710/jbt.1.2.38-43

Abstract

Chlorophyll is a pigment of photosynthesis which is also efficacious as a cure for brain, lung, and oral cancer, and can be used as a disinfectant, antibiotic and dietary supplement, while carotenoids are useful as antioxidants. The content of the two pigments is strongly influenced by the age of the plant, especially for vegetable crops used in leaves such as cowpea (Vigna unguiculata (L.) Walp.). The purpose of this research is to know the pattern of chlorophyll content and carotenoid of cowpea leaf during the growth phase and to know the age of the right plant for harvesting cowpea so that the highest chlorophyll and carotenoid content is obtained. Cowpea seeds obtained from seeds are added then planted in pots containing ready-made planting media in the greenhouse. Chlorophyll and carotenoid analyzes were performed at different plant ages from 1 to 11 weeks. The research design used is Completely Randomized Design. Data were analyzed using variance analysis and continued with Duncan test at 95% significance level. The results showed that the highest chlorophyll and carotenoid content was produced by the cowpea leaf in the vegetative phase or before the flowering time. The highest chlorophyll content was obtained at 4WAP (weeks after planting) of age crop, while the highest carotenoid content was obtained in 3WAP.  
Co-Authors Abdul Khafid, Abdul Ade Arisma Fauziah Ade Arisma Fauziah Agustin Noviati Aziz Purwantoro Desi Arisanti Diah Nurmaningrum Endah Dwi Hastuti Endang Saptiningsih Endang Semiarti Erma Prihastanti Ertina Novaria Sitorus Eva Septiyana Fakhri Fadhlurrohman Pratama Farida Nur Hasanah Fauziah, Ade Arisma Fella Suffah Meinaswati Fella Suffah Meinaswati Fetryani Soni Manurung Heny Irawati Ika Indah Selawanti Ika Susanti Hendriyani Jefri Saputro Luaeliyah, Masrukhatul Much. Azam Muhammad Faisol Hakim Muhammad Khusni Hidayat Muhammad Nur Munifatul Izzati NIDA, KHOIRIN Nita Kumalasari Dewi Nurullita Prahasti Prahasti, Nurullita Sri Darmanti Sri Widodo Agung Suedy Sukarti Moeljopawiro Sulistyowati Sulistyowati Surya Kurnia Hayati Tanti Dwi Romawati Ulva, Maria Wahyu Hadayani Yulita Nurchayati