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All Journal Majalah Kedokteran Bandung Medula Juke Indonesian Journal of Cancer Jurnal Aplikasi Teknologi Pangan Jurnal Pengabdian Pada Masyarakat Jurnal Aplikasi Teknologi Pangan Bioscientia Medicina : Journal of Biomedicine and Translational Research Review of Primary Care Practice and Education (Kajian Praktik dan Pendidikan Layanan Primer) Jurnal Mandala Pengabdian Masyarakat MAHESA : Malahayati Health Student Journal Bioscientia Medicina : Journal of Biomedicine and Translational Research
Asep Sukohar
Department Of Pharmacology; Faculty Of Medicine; Universitas Lampung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Dentistry Education Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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Journal : Indonesian Journal of Cancer

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Peran Asam Klorogenat Terhadap Ekspresi Gen; miRNA 146 A, Caspase 3, Cyclin D1, dan Kematian Sel Kanker Hepatoseluler Model Cell Lines Hep-G2 SUKOHAR, ASEP; HERAWATTI, HENING; WITARTO, ARIEF B
Indonesian Journal of Cancer Vol 8, No 1 (2014): Jan - Mar 2014
Publisher : "Dharmais" Cancer Center Hospital

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (636.82 KB)

Abstract

Chlorogenic acid (CA) is the active compound isolated from medicinal plants, can be used as a chemopreventive agents of hepatocellular cancer (HCC). It works as an exogenous antioxidant and inhibit growth of cancer cells through the inhibition of free radicals. The purpose of this study is to know mechanism of CA in inhibition to growth of Hep-G2 through apoptosis stimulation. In vitro study was performed Hep-G2 cells. The samples were divided into the control group treatment and experiment group expose to 727, 500 and 250 ?M and 3 times repetition. Expression of miRNA 146 A, caspase 3 and cyclin D1 examined by RT-PCR CFX-96. Samples were analysed at 0, 8, 18, and 24 hours after exposure CA. The data were statistically tested by repeated measurement, pearson, and multivariate regression. The results showed that cell death of Hep-G2 were increase as the dose increase and time, at 8 hours after exposure CA cell death of Hep-G2 increased as much as 35,68, 37,75, 40,86%. At 18 hours cell death of Hep-G2 increased as much as 54,56, 56,48, and 59,73%. At 24 hours cell death of Hep-G2 increased as much as 67,73, 69,37, and 72,16%. The lowest expression miRNA 146 A in group 24 hours after exposure at doses of 727 ?M CA (0,85), followed by 500 ?M (1,28) and the highest expression at a dose of 250 ?M (1,61). The result of repeated measurement test: miRNA 146 A and caspase 3 at 8th and 18th hours was significantly different to the 24th with p<0.05. The expression of caspase 3 increase from 0-24 hours, the highest expression of caspase 3 in group 18 hours after exposure at doses CA at 750 ?M (3,86). After 18 hours, expression caspase 3 decreased and the lowest in group 24 hours after exposure: at a dose of 250 ?M (1,52). Expression of cyclin D1 decrease from 0-24 hours with the highest expression at 0 hours (4,35) at a dose of 250 ?M and the lowest expression at 24 hours after exposure to CA (0,32) at a dose of 727 AK ?M.
Peran Asam Klorogenat Terhadap Ekspresi Gen; miRNA 146 A, Caspase 3, Cyclin D1, dan Kematian Sel Kanker Hepatoseluler Model Cell Lines Hep-G2 SUKOHAR, ASEP; HERAWATTI, HENING; WITARTO, ARIEF B
Indonesian Journal of Cancer Vol 8, No 1 (2014): Jan - Mar 2014
Publisher : Indonesian Journal of Cancer

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (636.82 KB)

Abstract

Chlorogenic acid (CA) is the active compound isolated from medicinal plants, can be used as a chemopreventive agents of hepatocellular cancer (HCC). It works as an exogenous antioxidant and inhibit growth of cancer cells through the inhibition of free radicals. The purpose of this study is to know mechanism of CA in inhibition to growth of Hep-G2 through apoptosis stimulation. In vitro study was performed Hep-G2 cells. The samples were divided into the control group treatment and experiment group expose to 727, 500 and 250 ?M and 3 times repetition. Expression of miRNA 146 A, caspase 3 and cyclin D1 examined by RT-PCR CFX-96. Samples were analysed at 0, 8, 18, and 24 hours after exposure CA. The data were statistically tested by repeated measurement, pearson, and multivariate regression. The results showed that cell death of Hep-G2 were increase as the dose increase and time, at 8 hours after exposure CA cell death of Hep-G2 increased as much as 35,68, 37,75, 40,86%. At 18 hours cell death of Hep-G2 increased as much as 54,56, 56,48, and 59,73%. At 24 hours cell death of Hep-G2 increased as much as 67,73, 69,37, and 72,16%. The lowest expression miRNA 146 A in group 24 hours after exposure at doses of 727 ?M CA (0,85), followed by 500 ?M (1,28) and the highest expression at a dose of 250 ?M (1,61). The result of repeated measurement test: miRNA 146 A and caspase 3 at 8th and 18th hours was significantly different to the 24th with p<0.05. The expression of caspase 3 increase from 0-24 hours, the highest expression of caspase 3 in group 18 hours after exposure at doses CA at 750 ?M (3,86). After 18 hours, expression caspase 3 decreased and the lowest in group 24 hours after exposure: at a dose of 250 ?M (1,52). Expression of cyclin D1 decrease from 0-24 hours with the highest expression at 0 hours (4,35) at a dose of 250 ?M and the lowest expression at 24 hours after exposure to CA (0,32) at a dose of 727 AK ?M.
Co-Authors Aditya Ramadhon Islami Sakaganta Afriyani Afriyani Andi Nafisah Anita Febrina Arief B Witarto Arli Suryawinata Arli Suryawinata Aulian Mediansyah Bainah Sari Dewi Endah Ambarwati Evi Kurniawaty Fibra Nurainy Hari Kaskoyo HENING HERAWATTI, HENING Herri S. Sastramihardja Ihsanti Dwi Rahayu Josi Jeremia Manurung Muhartono Muhartono Novita Herdiana Nur Fitriana Muhammad Ali Ramadhan Triyandi Ratna Dewi Puspita Sari Samsu Udayana Nurdin Samsul Bakri Sofyan Musabiq Wijaya Suharyani Sutarto Sutarto Winda Trijayanthi Utama, Winda Trijayanthi Yofita Sulfiana Sundari Zulpakor Oktoba