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Journal : Bioma : Berkala Ilmiah Biologi

Kadar Protein Daging Pada Keturunan Puyuh (Coturnix coturnix japonica L.) Betina (F1) Dari Induk Yang Diberi Suplemen Serbuk Kunyit Dalam Pakan Rifa'i, Ahmad; Saraswati, Tyas Rini; Tana, Silvana
Bioma : Berkala Ilmiah Biologi Vol. 18, No.2, Tahun 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (40.721 KB) | DOI: 10.14710/bioma.18.2.97-101

Abstract

This research aims to review determine protein level of meat tillers (F1) quails female breederwas treated supplemented turmeric powdersin feeds. This research using an experimental method with completely randomized design (CRD). The animals test used 45 tillers quail female its breederwas treated supplements turmeric powders, divided in 3 (three) treatment, i.e. K0: Tillersquails female which breeder was not treated supplements turmeric powders in feed. K1: Tillersquails female which breeder was treated turmeric powder 54 mg/head/day in feed. K2: Tillersquails female which breeder was treated turmeric powder 108 mg/head/day in feed. Tillersjapanese quail not given any treatment, only the standard feed and maintained until 60 days. Data is taken from meat protein content, daily feed intake and body weight. Data were analyzed with analysis of variance (ANOVA) with assisted program SPSS 16, the results show a real difference, then continued with Duncan test with 95% confidence level (α=0.05). The results showed that effect of turmeric powder on breeder can lower daily feed intake, but can increase the protein content of the meat, so the feed efficiency is increased in tillers japanese quails female of breeder was treated turmeric powder. Keywords: Coturnixcoturnix japonica L.,Meat protein content, Japanese quail, Turmeric powder.
Histologis Testis pada Keturunan F1 dari Induk Puyuh (Coturnix coturnix japonica L.) yang diberi Suplemen Serbuk Kunyit (Curcuma longa L.) dalam Pakan Waty, Mitra; Tana, Silvana; Saraswati, Tyas Rini
Bioma : Berkala Ilmiah Biologi Vol. 19, No. 1, Tahun 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (51.682 KB) | DOI: 10.14710/bioma.19.1.13-17

Abstract

Quail child has a tremendous potential to continue further descent because quails have almost all the potential that can be exploited by humans, either for consumption or economic. Children quail superior quality can be produced from superior quality parent, so the parent quail quality must be considered. Additional food in the form of powder turmeric supplements can increase the phytoestrogen which stimulates the liver to produce vitellogenin as the  material forming the yolk. Egg yolk is a source of nutrients for the development of quail embryos. This study aimed to analyze the differences in the quality of the parent male quail chicks were given supplements of turmeric powder to the testis weight, testis size, number of spermatogonia and spermatids amount contained in the seminiferous tubules. The study design used was completely randomized design (CRD) that is 3 treatments and 5 replications. By using quail male child as much as 15 tails as test animals, divided into three groups, namely K0: F1 generation of the female quail are not given turmeric powder supplement, K1: F1 generation of the female quail turmeric powder supplemented with a dose of 54 mg/tail/day and K2: kuturunan F1 of the parent quail turmeric powder supplemented with a dose of 108 mg/tail/day. The variables measured were testis weight, testis size, number of spermatogonia and spermatids number. Data were analyzed using Analysis of Variance (ANOVA) followed by a further test Duncan Multiple Range Test (DMRT) at the 95% significance level. The results showed that the quality of parent child quail turmeric powder supplemented with a dose of 108 mg/tail/day for better views of the process of spermatogenesis. Keywords: Child  quail  (Coturnix  coturnix  japonica.  L),  Turmeric,  Testis,  spermatogonia, spermatids.
Kadar Kolesterol Daging Pada Keturunan F1 Dari Induk Puyuh Jepang (Coturnix coturnix japonica L.) Yang Diberi Suplemen Tepung Kunyit (Curcuma longa L.) Dalam Pakan Andriani, Regita; Saraswati, Tyas Rini; Tana, Silvana
Bioma : Berkala Ilmiah Biologi Vol. 20, No 2, Tahun 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (61.456 KB) | DOI: 10.14710/bioma.20.2.86-91

Abstract

Quail is one of poultry commodities that compliance the needed of animal protein like egg and meat. Good quality was breed from quail parent that has good physiological condition. This research aimed to quantified cholesterol of quail meat from F1 from quail parent that treatment using turmeric powder supplement on itsfed in order to obtained best quail chick with good physiological condition. This research use Completely Randomized Design (CRD) experimental method. Animal on this research was 15 Japanese quail (Coturnix coturnix japonica L.) hens that gave 3 treatments with 5 repetitions. K0 was F1 of quail parent without turmeric powder supplementation, K1 was F1 of quail parent with 54 mg/quail/day turmeric powder supplementation before its sex period, K2 was F1 of quail parent with 108 mg/quail/day turmeric powder supplementation before its sex period. The F1 quail was fed using standard quail feed. Data was obtained from daily fed consumption average, daily water consumption, and quail meat cholesterol rate on 60 day old chicken. Data was analyses using Analysis of Variance (ANOVA). This research result showed that daily fed consumption, daily water consumption and meat cholesterol rate of F1 that treatment using turmeric powder supplementation wasn’t show significant difference, so conclusion of this research is physiological condition of F1 was normal. Key words: Quail Meat Cholesterol, F1 of Japanese quail (Coturnix coturnix japonica), Turmeric powder
Struktur Histologi Hepar Tikus Wistar (Rattus norvegicus) Jantan setelah Pemberian Teh Kombucha Konsentrasi 75% dengan Waktu Fermentasi yang Berbeda Isdadiyanto, Sri; Tana, Silvana
Bioma : Berkala Ilmiah Biologi Vol. 21, No 2, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (461.605 KB) | DOI: 10.14710/bioma.21.2.165-172

Abstract

This research was conducted to study and analyze the influence of kombucha fermentation 6 (P1), 9 (P2) and 12 (P3) days fermentation with 75% concentration on hepatic tissue structure and hepatocyte diameter. This study used 16 male Wistar rats consisted of 4 treatments and 4 repeat. The treatment of this study consisted of P0: control treatment; P1, P2 and P3 were treatment of kombucha fermented tea 6, 9 and 12 days of 75% concentration respectively. This research used Completely Randomized Design (RAL). The variables measured in this study were hepatocyte diameter, body weight, feed and drink consumption in animal test. The data obtained were analyzed using Analysis of Variance (ANOVA) at 95% confidence level. The result of data analysis showed that kombucha fermentation 6, 9 and 12 days of 75% concentration had no significant difference to all variables. Histology of the liver were not indicate the presence of tissue-damaged features such as chronic inflammation (cirrhosis). It could be concluded that tea kombucha was safe for consumption by mammalia because it was not affect liver damage.
Profil Histologi Duodenum Berbagai Itik Lokal Di Kabupaten Semarang Apriliyani, Nur Indah; Djaelani, Muhammad Anwar; Tana, Silvana
Bioma : Berkala Ilmiah Biologi Vol. 18, No.2, Tahun 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (53.441 KB) | DOI: 10.14710/bioma.18.2.144-150

Abstract

Local duck had been widely cultivated not only as a producer of eggs processed as salted egg but also there are cultivated as broiler ducks. The efforts to improve the productivity of ducks can be seen from the effectiveness of the process of absorption of nutrients in the duodenum, so the use of energy in the metabolic process can be optimized for adding mass and volume of the cell.  This study aims to determine the duodenum profile of the histological structure  covered the lumen diameter, villous height and number of goblet cells of Magelang ducks, Tegal ducks and Pengging ducks .This study used a completely randomized design (CRD) with three treatments (Magelang ducks, Tegal ducks and Pengging ducks) and 6 replications. The measured variable was the long intestine tenue, lumen diameter, villous height and number of goblet cells. Data were analyzed using analysis of variance (ANOVA) with a differentiation factor was the type of duck. The realdifferences data were analyzed using advanced test of Duncan at the level of 95%. The results showed long intestine tenue, lumen diameter and height of the villi were not significantly different at each Magelang ducks, Tegal ducks, Pengging ducks. The number of goblet cells in Magelang ducks and Tegal ducks showed significantly different, but not on Pengging Ducks. The conclusion of the study was the length of the intestine tenue, lumen diameter and height of villous had no real effect on three types of ducks. Keywords: Local ducks, duodenum, histological
Pertumbuhan Kelinci (Lepus spp.) Setelah Pemasangan Implan Stainless Steel AISI 316L Wicaksono, Wisnu Adi; Tana, Silvana; Mardiati, Siti Muflichatun
Bioma : Berkala Ilmiah Biologi Vol. 18, No.2, Tahun 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (116.147 KB) | DOI: 10.14710/bioma.18.2.137-143

Abstract

A lot of accidents can cause fractures/cracks, especially in the thigh bone. Bone fractures/cracks can be overcome through implants installation (implantation) particularly on the part of the femur. A test take the implant integration process about 4-6 week. Research on animals is performed the rabbit (Lepus spp.) implantation of stainless steel AISI 316L. The implantationof stainless steel AISI 316L implant was on the right femur between thigh bone and sceletal muscle, that was observed for 1,5 months. Parameters observed in this research were body weight gain, feed consumption, conversion of feed, and drinking consumption. Test used is t two test samples independent two sides with take samples 5 control and 5 treatment stainless steel AISI 316L. The results show the stainless steel AISI 316L implantsignificant differences body weight gain, feed consumption, and drinking consumption,but no significant different feed conversion. The conclusion is an implant stainless steel AISI 316L influence body weight gain rabbits after implantation for 1.5 months. Keywords: Rabbit, stainless steel AISI 316L, growth, body weight
Struktur Mikroanatomi Testis Tikus Putih (Rattus norvegicus) Setelah Pemberian Teh Kombucha Konsentrasi 75% Waktu Fermentasi 6, 9 Dan 12 Hari Isdadiyanto, Sri; Tana, Silvana
Bioma : Berkala Ilmiah Biologi Vol. 21, No 1, Tahun 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (362.592 KB) | DOI: 10.14710/bioma.21.1.78-85

Abstract

The objective of this study was to analyze the testicular microanatomy structure of Rattus norvegicus after treatment by 75% kombucha tea with variation of fermentation time. Sixteen male of rats, 2 months of age were used as experimental animals treated with kombucha tea that had been fermented for 6, 9 and 12 days at the temperature of 25oC per oral. This research was using completely randomized design with 4 treatment (for 4 weeks) and 4 replications, namely: P0 = control, without of kombucha tea, P1 = water + 1,8 ml kombucha tea that had been fermented for 6 days in the morning and afternoon, P2 = water + 1,8 ml kombucha tea that had been fermented for 9 days in morning and afternoon, P3 = water + 1,8 ml kombucha tea that had been fermented for 12 days in morning and afternoon. The variables measured were seminiferous tubule diameter and Leydig cell counts. Data were analyzed with ANOVA followed by Duncan test at 95% confidence level were performed using SPSS version 16,0. The results showed that kombucha tea decreased the diameter of the seminiferous tubule, showing the potention of spermatogenesis disturbance, but did not affect Leydig cell counts of Rattus norvegicus
Pengaruh Pemberian Teh Kombucha Konsentrasi 50% dengan Waktu Fermentasi yang Berbeda pada Struktur Histologi Hepar Tikus Wistar (Rattus norvegicus) Jantan Isdadiyanto, Sri; Tana, Silvana
Bioma : Berkala Ilmiah Biologi Vol. 23, No 1, Tahun 2021
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.23.1.51-56

Abstract

This study was conducted to determine the effect of 6 kombucha fermented tea (P1), 9 (P2) and 12 (P3) fermentation days at a concentration of 50% on the liver histological structure and hepatocyte diameter. This study used 16 male white rats consisting of 4 treatments and 4 replications. The treatment of this study consisted of P0: control treatment; P1, P2 and P3 respectively were given 6, 9 and 12 days fermented kombucha tea at a concentration of 50%. This research uses a completely randomized design. The variable measured in this study was the diameter of hepatocytes in test animals. The data obtained were analyzed using Analysis of Variance (ANOVA) at a 95% confidence level and if there were real differences, followed by Duncan's test. The results of data analysis showed that kombucha tea fermented 6, 9 and 12 days with a 50% concentration did not have a significant difference in hepatocyte diameter variables. The histological structure of the liver looks normal and shows no sign of cell damage. It can be concluded that kombucha tea at a concentration of 50% is safe for consumption by mammals because it does not cause liver damage.
Kualitas Kerabang Telur pada Berbagai Itik Petelur Lokal di Balai Pembibitan dan Budidaya Ternak Non Ruminansia (BPBTNR), Ambarawa Eka Fitriani; Sri Isdadiyanto; Silvana Tana
Bioma : Berkala Ilmiah Biologi Vol. 18, No.2, Tahun 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (52.707 KB) | DOI: 10.14710/bioma.18.2.107-113

Abstract

Duck (Anas platyrhynchos) was one type of potential poultry producing eggs. Efforts to increase productivity can be done through the selection of good breeding stock, provision of adequate food in quantity and quality as well as the provision of additional feed (feed additive). Calcium carbonate was a mineral needed by a group of ducks for egg shell formation. The purpose of the research was quality of the local duck eggs Pengging include ducks, duck Tegal and duck Magelang  based on the quality of its shell. This study uses a completely randomized design (CRD) with three treatments (Pengging duck, duck Ducks Tegal and Magelang) and 6 replication.The measured variable was the index of egg shell, eggshell weight, eggshell thickness and eggshell calcium. Data were analyzed using Analysis of Variance (ANOVA) with a differentiating factor is the type of duck. Real different data analyzeda further test using Least Significant Differences Test (LSDT) at the level of 95%. The results of this research indexshowed that ducks Tegaland duck Pengging no significant difference but significantly different with Magelang ducks. Weight eggshell and eggshell thickness showed no significantly different for the three types of local ducks. Eggshell calcium levels showed that Magelang ducks and duck Pengging no significant difference but significantly different with Tegal ducks.Conclusion of the study was index eggshell and eggshell calcium could affect the quality of the eggshell. Keywords: Local Ducks in Central Java, the index of egg shell, eggshell weight, eggshell thickness, eggshell calcium.
Respon Histologis Aorta dan Jantung Rattus Norvegicus Hiperlipidemia Setelah Pemberian Jus Buah Kersen (Muntingia calabura L.) dan Ekstrak Daun Lakum (Cayratia trifolia L.) Enyda Agustina; Tyas Rini Saraswati; Silvana Tana
Bioma : Berkala Ilmiah Biologi Vol. 24, No 2, Tahun 2022
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.24.2.96-104

Abstract

This study aims to determine the histological features of the aorta and heart of male Rattus norvegicus after  administration of cherry juice (Muntingia calabura L.) dan lakum leaf extract  (Cayratia trifolia L.). The study used 30 male Rattus norvegicus L.  in a Completely Randomized Design (CRD) consisting of 5 treatments with 6 repetitions, namely the control group (P0), the standard feeding group (P1), the hyperlipid feed group + 0.2 mL/200 g BB cherry juice (P2), the hyperlipid feed group + 40 mg / 200 g BB lakum leaf extract (P3) and hyperlipid feed group + 0.18 mg / 200 g BB simvastatin (P4). Hyperlipid feed was given during the treatment, which was 28 days. To obtain histological data, preparations were made using the paraffin method and then observed using a photomicrograph with 400x magnification and observing the aortic lumen, foam cells and intracellular accumulation of lipids in smooth muscle. Data were analyzed by ANOVA followed by Duncan Test at a significant level of 5%. The results showed that the addition of cherry juice and lakum leaf extract gave a significant effect on lumen width and thickness of the aortic wall (p <0.05), while on changes in heart muscle cell size no significant effect (p> 0.05). Histomorphometri showed the presence of foam cells and accumulation of smooth muscle lipids in the control and group treatments with hyperlipid feeding. The conclusion of this study was that the addition of cherry juice  and lakum leaf extract can reduce atherosclerotic lesions by eliminating showed foam cells and prevent proliferating smooth muscle cells so the size of the aortic lumen width returns to normal, but didn’t cause enlargement of the heart muscle (cardiomegaly).