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All Journal Jurnal Ilmu Ternak Microbiology Indonesia Jurnal Biologi dan Pembelajarannya (JBP) Jurnal Riset Kimia Jurnal Agribisnis Terpadu
Pingkan Aditiawati
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary

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The Effect of Mutation at Thr 295 of Saccharomyces cerevisiae eRF1 on Suppression of Nonsense Codons and eRF1 Structure PRIMA ENDANG SUSILOWATI; PINGKAN ADITIAWATI; FIDA MADAYANTI; . AKHMALOKA
Microbiology Indonesia Vol. 2 No. 1 (2008): April 2008
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.2.1.3

Abstract

The termination of translation in Saccharomyces cerevisiae is controlled by two interacting polypeptide chain release factors, eRF1, and eRF3. Two regions in eRF1, at position 281-305 and 411-415, were proposed to be involved on the interaction to eRF3. In this study we have constructed and characterized eRF1 mutants at position 295 from threonine to alanine and serine residues resulting in eRF1(T295A) and eRF1(T295S) respectively. The mutations did not affect the viability or temperature sensitivity of the cells. The stop codons readthrough of the mutants were analyzed in vivo using PGK-stop codon-LACZ gene fusion and the results showed that thesuppression of the mutants was increased in all of the codon terminations. The suppression of the UAG codon was the high for both mutants, with a 7-fold increased for eRF1(T295A) and a 9 fold increase for eRF1(T295S). The suppressor activity of eRF1(T295S) was higher compared to that of eRF1(T295A), suggesting that the accuracy of translational termination in eRF1(T295S) was lower than that of eRF1(T295A). Computer modeling analysis using Swiss-Prot and Amber version 9.0 programs revealed that the overall structure of eRF1 mutants has no significant difference with the wild type. However, substitution of threonine to serine on eRF1(T295S) triggered a secondary structure change on the other motif of the C-terminal domain of eRF1. This observation did not occur for on eRF1(T295A). This suggests that the high stop codon suppression on eRF1(T295S) is probably due to the slight modification of the structure of the C terminal motif.
Pengembangan Minuman Probiotik dari Buah Kawista (Feronia limonia) dengan Bakteri Asam Laktat Indigenous elysabet herawati; pingkan aditiawati
Jurnal Biologi dan Pembelajarannya (JB&P) Vol 2 No 1 (2015): Jurnal Biologi dan Pembelajarannya
Publisher : Universitas Nusantara PGRI Kediri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29407/jbp.v2i1.329

Abstract

Kawista (Feronia limonia) merupakan tanaman suku jeruk-jerukan (Rutaceae) berpotensi sebagai tanaman obat. Menurut penelitian yang pernah dilaksanakan, buah kawista baru diolah menjadi sirup, dodol, selai dan madumongso. Semakin meningkatnya perhatian terhadap pengaruh makanan dan minuman terhadap kesehatan, memicu berkembangnya produk kesehatan dengan pemanfaatan bahan alami. Berdasarkan manfaat buah kawista, penelitian bertujuan untuk mengembangkan produk sirup buah kawista sebagai minuman probiotik dengan pemanfaatan bakteri indigenous genus Lactobacillus yang diisolasi dari buah kawista. Pembuatan minuman probiotik kawista diawali dengan pembuatan sirup dengan metode maserasi. Bakteri  indigenous diisiolasi dari kulit luar, kulit dalam dan sirup buah kawista dengan teknik pengenceran bertingkat. Isolasi dilakukan pada media NA yang dimodifikasi dengan sirup kawista. Sebanyak 35 isolat bakteri yang didapat diseleksi dengan pemindahan ke media MRS. Dilakukan uji metode difusi sumur untuk mengetahui aktivitas antimikroba isolat terhadap bakteri patogen pencernaan yakni Bacillus subtilis, Escherichia coli dan Staphylococcus aureus. Diamati zona bening yang terbentuk pada 3 bakteri uji sehingga didapatkan 3 isolat bakteri terpilih dengan diameter zona 15,5 mm; 17 mm dan 30,5 mm. Isolat terpilih didentifikasi dengan metode  perwarnaan gram sehingga diketahui jenis bakteri adalah gram positif. Identifikasi molekuler dilakukan oleh Macrogen-Korea. Dari hasil sekuensing didapatkan spesies Lactobacillus paracasei strain FT179 sebagai isolat fermentasi. Dilakukan pembuatan kurva baku dan kurva tumbuh untuk mengetahui pertumbuhan optimal sebagai acuan pembuatan starter. Waktu pertumbuhan optimum Lactobacillus paracasei didapatkan pada jam ke 12.
PENGARUH PELATIHAN TEKNOLOGI PESTISIDA LIMBAH TEMBAKAU TERHADAP PERILAKU PETANI Pingkan Aditiawati; Mia Rosmiati; Dadang Sumardi
JURNAL AGRIBISNIS TERPADU Vol 8, No 2 (2015): Jurnal Agribisnis Terpadu
Publisher : Jurusan Agribisnis Fakultas Pertanian Universitas Sultan Ageng Tirtayasa

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (68.222 KB) | DOI: 10.33512/jat.v8i2.1307

Abstract

The purpose of the training is to socialize and provide comprehension to farmers about technologyinnovation of botanical pesticide from tobacco waste. Farmer- behavior change is expected to lead tofarmers’ desire to apply technological innovation of botanical pesticide. This research useddescriptive method. Determination of respondent is conducted by a census of all the farmers whoattended training on botanical pesticide technology (20 respondents). Indicators of farmer- behaviorchanges including cognitive, affective, and psychomotor about the benefits, how to make and apply thebotanical pesticide. The indicators are measured using a Likert scale. Data were analyzed usingdescriptive statistics. The results showed that the training of botanical pesticide technology has a goodinfluence on the behavior of farmers.
16S Ribosomal RNA-Based Analysis of Thermophilic Bacteria in Gedongsongo Hot Spring AGUSTINA LULUSTYANINGATI NURUL AMININ1; FIDA MADAYANTI; PINGKAN ADITIAWATI; . AKHMALOKA
Microbiology Indonesia Vol. 1 No. 1 (2007): April 2007
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.1.1.9

Abstract

Denaturing gradient gel electrophoresis was used to identify the bacterial community at the Gedongsongo (WGS-2) hot spring. The bacterial samples were obtained from both culture dependent and independent strategies. Partial 16S rRNA genes were amplified by a set of primers to produce at around 400 bp fragments, including the highly variable V9 region of the 16S rRNA genes. The DGGE profiles showed that there were a few distinct bands, namely G1-G3, and G8-G12, which represent the predominant bacteria in natural habitat and the medium.Further analysis of these bands showed that most of them, except for G7, have a high homology to the 16S rRNA gene sequences of Thermus sp. As for G7, the highest homology was shown to unculturable bacteria. In addition to the distinct bands in DGGE, there were other three thin bands, namely G4, G5, and G6, which possibly represent non dominant microorganisms in the natural habitat, but could grow on GS-A medium. Further analysis of these bands showed that G6 has 80% similarity to the 16S rRNA of Burkholderia sp., while G4 and G5 have a high homology to each other but only contained 10-15% homology to the sequences of 16S rRNA from unculturable microorganisms. The phylogenetic analyses of the last organisms showed that there was branching from Burkholderia. From all the data obtained it was suggested that the WGS-2 hot spring was predominantly occupied by the genus Thermus. In addition, there were a few novel microorganisms found in the hot spring.
Pengaruh Silase Sinambung Jerami Jagung Terhadap Fermentasi Dalam Cairan Rumen Secara In Vitro Crhisterra Ellen Kusumaningrum; Irawan Sugoro; Pingkan Aditiawati
Jurnal Ilmu Ternak Vol 18, No 1 (2018): June
Publisher : Fakultas Peternakan, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (220.995 KB) | DOI: 10.24198/jit.v18i1.14460

Abstract

Penelitian dilakukan untuk mengevaluasi pengaruh penggunaan silase sinambung jerami jagung terhadap, nilai pH, volatile fatty acid (VFA), amonia (NH3), produksi gas total dan produksi biomassa mikroba sebagai pakan ternak ruminansia secara in vitro. Rancangan percobaan dalam penelitian ini adalah Rancangan Acak Lengkap Pola Faktorial 3x6 dengan 3 ulangan. Faktor pertama dalam rancangan penelitian ini adalah pakan perlakuan yaitu pakan kontrol (P1), jerami jagung (P2) dan silase sinambung jerami jagung (P3) dan faktor kedua adalah waktu inkubasi selama fermentasi in vitro yaitu 0, 2, 4, 6, 12 dan 24 jam (T1, T2, T3, T4, T5 dan T6). Hasil penelitian menunjukkan bahwa baik pakan perlakuan maupun waktu inkubasi tidak memberikan pengaruh yang nyata (p>0,05) terhadap nilai pH, VFA, NH3, produksi gas total dan produksi biomassa mikroba serta tidak terdapat interaksi antara perlakuan pakan dengan waktu inkubasi. Secara numerik, kisaran pH perlakuan adalah 6,89 - 7,05; konsentrasi VFA sebesar 107–110 mM, konsentrasi amonia 23,92 – 29,88 mg/100 ml, produksi gas total sebesar 27,47 – 46,31 ml/200 mg dan produksi biomassa mikroba sebesar 40,60 – 56,80 mg/20 ml. Kesimpulan pada penelitian ini adalah baik jerami jagung maupun silase sinambung jerami jagung dapat digunakan sebagai pakan ternak karena mampu memenuhi kebutuhan nutrisi ternak ruminansia ditinjau dari produksi gas dan fermentasi pakan dalam rumen.Kata kunci: silase sinambung jerami jagung, amonia, VFA, produksi gas in vitro.
PRODUKSI PROTEASE ALKALI DAN KERATINASE DARI Brevibacillus agri A-03 TERMOFILIK Anthoni Agustien; Jetty Nurhajati; Linar Z. Udin; Pingkan Aditiawati
Jurnal Riset Kimia Vol. 4 No. 1 (2010): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v4i1.44

Abstract

 ABSTRAK Protease alkaline and keratinase are a group of protease enzym which have important value in detergen industry and skin tannery. Brevibacillus agri A-03 is thermophilic bacteria isolate that comes from Ambayan Sumatera Barat hot spring and has the ability to produce protease and keratinase. The purpose of this research is to get protease alkaline and thermostable keratinase from Brevibacilus agri-A03. thermostable enzym is produced from enzym production enzym that contains kasein and keratin at various medium pH, inoculum incubation temperature and medium type. Enzym activity is measured by modified Walker methode, protein content is measured by Lowry methode. Protease alkaline is produced at exponential phase, maximum at 18th hours of incubation and keratinase is produced at stationer phase, maximum at 22nd hours. Both enzym is produced optimically at medium pH condition 9.0; incubation temperature 55°C, inoculum 5% by using modified Johnvesly and Naik medium with each protease and keratinase specific activity 1.927 and 1.047 U/mg  Keywords: Protease alkaline, Keratinase, Thermofilic, Brevibacillus agri A-03   
Cell Lysis Method Affects Assessment of Microbial Diversity Based on Ribotyping Analysis HENI YOHANDINI; FIDA MADAYANTI; PINGKAN ADITIAWATI; . AKHMALOKA
Microbiology Indonesia Vol. 2 No. 1 (2008): April 2008
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5454/mi.2.1.6

Abstract

The microbial community in Kawah Hujan, Kamojang, West Java, Indonesia, was analyzed using 16S-rRNA-gene-sequencing combining with denaturing-gradient-gel electrophoresis (DGGE) technique. Two different cell lysis methods, enzymatic-based, and physical treatment-based DNA extraction, were used to isolate chromosomal DNA for 16S rDNA gene-fragment amplification. The DGGE profiles showed some differences in banding pattern that were obtained from both cell lysis methods. The DNA sequence analysis of the individual DGGE bands revealed that most of the band sequences obtained by physical treatment were close to 16S rRNA gene fragments from the bacterial domain, while most of band sequences performed by enzymatic method had high homology with 16S rRNA gene fragments from archaeal domain. Further analysis of the sequences from both methods performed by comparisons with the Ribosomal Database Project showed that some of DGGE sequences from Kawah Hujan consisted unique 16S rDNA sequences.
Co-Authors . AKHMALOKA Agustina L.N. Aminin Anthoni Agustien Crhisterra Ellen Kusumaningrum Dadang Sumardi Elysabet Herawati FIDA MADAYANTI Heni Yohandini Irawan Sugoro Jetty Nurhajati Linar Z. Udin Mia Rosmiati PRIMA ENDANG SUSILOWATI