Diyah Martanti, Diyah
Pusat Penelitian Biologi-LIPI, Cibinong Science Centre, Jl. Raya Bogor Km. 46, Cibinong 16911

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INDUKSI MUTASI KULTUR IN VITRO Amorphophallus muelleri Blume DENGAN IRRADIASI GAMMA Poerba, Yuyu S; IMELDA, MARIA; WULANSARI, AIDA; MARTANTI, DIYAH
Jurnal Teknologi Lingkungan Vol. 10 No. 3 (2009)
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (149.166 KB) | DOI: 10.29122/jtl.v10i3.1482

Abstract

Amorphophallus muelleri Blume (Araceae) is valued for its glucoman content for use infood industry (heathy diet food), paper industry, pharmacy and cosmetics. The cultivationof A. muelleri is hampered by limited genetic quality of seed. The species is triploid(2n=3x=39), the seed is developed apomictically, and pollen production is low. Thespecies is only propagated vegetatively. This may explain that the species is difficultto breed conventionally and genetic variability in the existing landraces cultivars israther limited. Conservation of this species, therefore, is important for availability of thespecies in the future use of this plant. The objective of present research is to increasegenetic variation by induce mutation using gamma-rays irradiation of shoot culturesof A. muelleri and to identify DNA polymorphism induced by gamma irradiation usingrandom amplified polymorphic DNA (RAPD), so the mutants produced can be used forbreeding purposes and for conservation program. Results of the experiment showedthat gamma irradiation less than 5 gray was effective to induce mutation of A. muelleri.Four RAPD primers generated 35 scorable bands with 100% polymorphic bands. Sizeof the bands varied from 350bp to 2.0kbp. Clustering analysis was performed based onRAPD profiles using the UPGMA method. The range of genetic distance among individualgenotypes was from from 0.00 to 0.72, while genetic variance of the population was0.21 + 0.13. The eighteen genotypes were proof to be mutants. The mutants producedin this experiment could be used as new germplasms for breeding purposes as well asfor use in conservation strategy
ANALISA GENETIK PISANG HIBRID DIPLOID BERDASARKAN MARKA RAPD (Random Amplified Polymorphic DNA) [Genetic Analysis of Diploid Banana Hybrids Based on RAPD Markers] Martanti, Diyah; Poerba, Yuyu Suryasari; Herlina, Herlina
BERITA BIOLOGI Vol 16, No 3 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3363.503 KB) | DOI: 10.14203/beritabiologi.v16i3.2263

Abstract

Banana breeding by crosses is one way to get new cultivars with a better quality. This study aims to verify molecular characteristic of diploid hibrid bananas i.e MDRK, SNMT and MDMT using Random Amplified Polymorphic DNA (RAPD) markers. Ten RAPD primers have been selected to detect the differences in molecular pattern from all three diploid hybrid bananas. The results showed that the hybrid combination of MDRK, SNMT and MDMT produced bands that shared some similarities with each parents by 43.83%, 51.25% and 47.21% respectively as the number of bands from type I, III and IV markers. Type III marker is the important marker for identification of male parents. Type VII marker (non-parent bands) was detected from hybrid MDRK, SNMT and MDMT by 18 (20.22%), 6 (7.5%) and 11 (12.64%) respectively. Marker of type VII can be used to identified hybrid because they are present only in offspring. The highest polymorphic bands was obtained by SNMT (83.9%) and the lowest obtained was performed by MDMT (71.25%). From this result, RAPD markers can be used as a tool to detect the differences of molecular pattern of diploid hybrid bananas.
Karakterisasi Pisang Hibrid Tetraploid ‘GRNK’ Hasil Persilangan Pisang Triploid ‘Goroho’ dengan Pisang Iiar Diploid Musa acuminata Colla var. nakaii Nasution Poerba, Yuyu S.; Martanti, Diyah; Handayani, Tri; Witjaksono, Witjaksono
JURNAL BIOLOGI INDONESIA Vol 13, No 2 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2794.062 KB) | DOI: 10.14203/jbi.v13i2.3393

Abstract

ABSTRACTThe use of tetraploid banana in triploid banana breeding has been conducted through 4x x 2x crossing and/or its reciprocals, 2x x 4x crossing. However, the availability of tetraploid banana is naturally very limited, therefore artificially induced tetraploid banana is needed. The present research is aimed to generate tetraploid banana plants by crossing triploid banana ‘Goroho’ as female parent with wild diploid banana, Musa acuminata var. nakaii Nasution as male parent, and to characterize the tetraploid hybrid. 94 crossings were made by pollinating stigma of Pisang Goroho with polen of M. acuminata var. nakaii. Embrios resulted from hybridization were in vitro rescued and propagated. Hybrid plantlets were then acclimatized in the green house and seedlings were planted in the field for two cycles of banana reproduction. Ploidy identification, morphology and molecular characteristics were also conducted for two cycles of banana reproduction. Results of the experiment showed that 9 hybrid seeds were produced from 94 pollinations. However, only 2 hybrid embryos grew and developed into shoots and from two shoots only one shoot was survived. The one shoot were then in-vitro propagated to produce 21 hybrids planted in the field. After being evaluated for two plant reproductive cycles, all hybrid plants were tetraploids (2n=4x=44). The hybrid plants had drooping leaves, bigger fruit diameter compared to both parents, and blunt fruit tip. DNA (RAPD) profiles of tetraploid hybrid showed that 37.5% of the bands were from female parent, 37.5% from male parents, and 25% from both parents. The tetraploid hybrid ‘GRNK’ is expected to be useful in triploid banana breeding in the future.Key Words: Hybrid banana, tetraploid, Goroho, Musa acuminata Colla var. nakaii Nasution
ANALISA KESTABILAN GENETIK PISANG KEPOK ‘UNTI SAYANG’ HASIL MIKROPROGASI DENGAN MARKA RAPD DAN ISSR [Genetic Stability Analyses of Micropropagated Pisang Kepok ‘Unti Sayang’ by RAPD and ISSR Markers] Poerba, Yuyu Suryasari; Imelda, Maria; Martanti, Diyah
BERITA BIOLOGI Vol 11, No 2 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (206.276 KB) | DOI: 10.14203/beritabiologi.v11i2.497

Abstract

Random Amplified Polymorphic DNA (RAPD) and Inter-Simple Sequence Repeats (ISSR) markers were used to evaluate the genetic stability of micropropagated plants of ‘Pisang Kepok Unti Sayang’ at various stage of in vitro sub-cultures and in vivo plant material.All RAPD and ISSR profiles from micropropagated plants were monomorphic and similar to those of field grown control plants until stage tenth of sub cultures (V1S10). No variation was detected within the micropropagated plants, except for C12 (V1S44),G7 (V1S48 ), I11 and I12 (V1S10).RAPD and ISSR marker were both could be used to test the genetic stability of micropropagated bananas using the developed protocol.
PENGARUH MUTAGEN ETIL METAN SULFONAT (EMS) TERHADAP PERTUMBUHAN KULTUR IN VITRO ILES-ILES {Amorphophallus muelleri Blume) Poerba, Yuyu S; Leksonowati, Aryani; Martanti, Diyah
BERITA BIOLOGI Vol 9, No 4 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (916.437 KB) | DOI: 10.14203/beritabiologi.v9i4.2013

Abstract

Amorphophallus muelleri Blume (Araceae) is one of 27 Amorphophallus species occur wild in Indonesia (Sumatera, Java, Floresand Timor). The species is valued for its glucoman content for use in food industry (heathy diet food), paper industry, pharmacyand cosmetics. The cultivation of A. muelleri is hampered by limited genetic quality of seed. The species is triploid (2n=3x=39),the seed is developed apomictically. and pollen production is low. The species is only propagated vegetatively. This may explainthat the species is difficult to breed conventionally and genetic variabillity in the exiting landaraces cultivars is rather limited.Induced mutation using ethyl methan sulfonate is one of techniques to increase genetic variation. The present research is aimed todetermine Lethal Dosage (LD) 50% and 75% of EMS and to study effects of EMS on growth of A, muelleri in vitro cultures for usein induced mutation program. Results of the experiment showed that LD-50 and LD-75 was observed at 0.875% EMS and 0.5%EMS. respectively. Number of shoot, and percentage of rooting culture were decreasing as EMS level concentration increases.
ANALISIS KERAGAMAN GENETIK KENTANG HITAM Herlina, Herlina; Poerba, Yuyu S.; Martanti, Diyah; Ahmad, Fajarudin; Yulita, Kusumadewi Sri
BERITA BIOLOGI Vol 13, No 2 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i2.686

Abstract

Kentang hitam [Plectranthus rotundifolius (Poiret) Sprengel] of family Lamiaceae is a minor root crop known only for people living in some parts of Java, Bali and Madura. It was rarely found in its natural habitat, thus it was assumed to have low level of genetic diversity. This present study aimed to assess genetic diversity of 63 accessions of kentang hitam from provenances of Java based on Inter Simple Sequence Repeats (ISSR) and Random Amplified Polymorphic DNA (RAPD) fingerprints. Ten primers of ISSR and RAPD were initially screened and eight were selected for the analysis. These eight primers (OPA13, OPB10, OPB13, OPD8, OPN14, UBC 807, 834 and 835) generated 61 bands with an average of 7.63 polymorphic fragment per primer. Percentage of polymorphism ranged from 8.20% (UBC 807 and 834) to 16.39% (OPB 10) with an average of 12.50%polymorphism.Clustering analysis was performed based on ISSR and RAPD profiles using the neighbour joining method and Principle Coordinate Analysis (PCO). The range of genetic similarity among accessions was 51-100% to which most of the accessions were clustered with more than 80% similarity. This confirmed our hypothesis of the low level of variation existed among accessions.
KARAKTERISASI PISANG HIBRID TETRAPLOID ‘GRNK’ HASIL PERSILANGAN PISANG TRIPLOID ‘GOROHO’ DENGAN PISANG IIAR DIPLOID MUSA ACUMINATA COLLA VAR. NAKAII NASUTION Poerba, Yuyu S.; Martanti, Diyah; Handayani, Tri; Witjaksono, Witjaksono
JURNAL BIOLOGI INDONESIA Vol 13, No 2 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v13i2.3393

Abstract

ABSTRACTThe use of tetraploid banana in triploid banana breeding has been conducted through 4x x 2x crossing and/or its reciprocals, 2x x 4x crossing. However, the availability of tetraploid banana is naturally very limited, therefore artificially induced tetraploid banana is needed. The present research is aimed to generate tetraploid banana plants by crossing triploid banana ?Goroho? as female parent with wild diploid banana, Musa acuminata var. nakaii Nasution as male parent, and to characterize the tetraploid hybrid. 94 crossings were made by pollinating stigma of Pisang Goroho with polen of M. acuminata var. nakaii. Embrios resulted from hybridization were in vitro rescued and propagated. Hybrid plantlets were then acclimatized in the green house and seedlings were planted in the field for two cycles of banana reproduction. Ploidy identification, morphology and molecular characteristics were also conducted for two cycles of banana reproduction. Results of the experiment showed that 9 hybrid seeds were produced from 94 pollinations. However, only 2 hybrid embryos grew and developed into shoots and from two shoots only one shoot was survived. The one shoot were then in-vitro propagated to produce 21 hybrids planted in the field. After being evaluated for two plant reproductive cycles, all hybrid plants were tetraploids (2n=4x=44). The hybrid plants had drooping leaves, bigger fruit diameter compared to both parents, and blunt fruit tip. DNA (RAPD) profiles of tetraploid hybrid showed that 37.5% of the bands were from female parent, 37.5% from male parents, and 25% from both parents. The tetraploid hybrid ?GRNK? is expected to be useful in triploid banana breeding in the future.Key Words: Hybrid banana, tetraploid, Goroho, Musa acuminata Colla var. nakaii Nasution
Embryo and Seed Germination of Pisang Klutuk Wulung (Musa balbisiana Colla) After Storage Kurniajati, Wulan Septiningtyas; Rahayu, Resa Sri; Prawestri, Apriliana Dyah; Zaelani, Ahmad; Martanti, Diyah; Herlina, Herlina; Poerba, Yuyu Suryasari
Biosaintifika: Journal of Biology & Biology Education Vol 14, No 1 (2022): April 2022
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v14i1.32377

Abstract

Pisang Klutuk Wulung is one of Musa balbisiana accessions which has potential for genetic improvement of cultivated banana so that its conservation is essential. This research aimed to study the post-storage germination of Indonesian seeds of Pisang Klutuk Wulung. Storage methods were carried out using a factorial completely randomized design with three factors: packing methods using vacuum and non-vacuum plastic bags; storage temperature at 25ºC, 4ºC, and -20ºC; and storage duration by 7, 14, 30, and 50 days. The germinations were done in vitro and ex vitro. The results showed that seeds stored at 25ºC in non-vacuum plastic bags were infested by molds, contrasting to the non-vacuum treatment. The sterilization method using 25% sodium hypoclorite, Tween 20, and 80% alcohol resulted in less contamination than 96% alcohol. In vitro germination from the vacuum treatment had a higher germination rate than non-vacuum treatment. However, ex vitro germination was not affected by the storage method. Similar patterns were seen in vitro and ex vitro germination as storage in 4ºC resulted in better seed germination after 30 and 50 days. In contrast, at -20ºC, no embryo germinated in all storage duration treatments. Pisang Klutuk Wulung seeds could not be stored in the long term as they rapidly lost their viability. Our finding showed that airtight condition by vacuum treatment and low temperature at 4ºC were able to maintain seed viability for longer period of storage. Thus, this finding was useful to improve Musa breeding programs and as an essential step for the long-term conservation of Musa genetic resources.