Article Per Year (5 Year)
p-Index From 2019 - 2024
1.259
P-Index
This Author published in this journals
All Journal SAINSMAT bionature Indonesian Journal of Biotechnology Biology Teaching and Learning Seminar Nasional Pengabdian Kepada Masyarakat SAINSMAT: Jurnal Ilmiah Ilmu Pengetahuan Alam Indonesian Journal of Fundamental Sciences
Hartono
Jurusan Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Negeri Makassar
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Education Electrical & Electronics Engineering Energy Engineering Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Physics Public Health Veterinary

Published : 21 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 21 Documents
Search

 1   2   3 
Parameter Kualitas Limbah Padat Rumah Potong Hewan Tamangapa Kota Makasasar Sebagai Bahan Baku Pembuatan Pupuk Kompos ., Hartono; Hiola, St. Fatma; Nur, Surahman
bionature Vol 15, No 2 (2014): Oktober
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (451.014 KB) | DOI: 10.35580/bionature.v15i2.1561

Abstract

Tujuan dari penelitian ini adalah untuk mengetahui parameter kualitas limbah padat Rumah Potong Hewan (RPH) Tamangapa. Sampel penelitian berupa isi rumen dan kotoran sapi diambil dari RPH Tamangapa. Data kuantitatif meliputi N-total (%) diukur dengan metode Kjeldahl, P-total (%) dengan metode Spektrofotometri, K-total (%) dengan metode AAS, C-Organik (%) dengan metode Chumies, rasio C/N dengan metode kalkulasi, kadar air (%) dengan metode oven dan bahan organik (%) dengan metode pengabuan. pH diukur dengan menggunakan pH meter. Data kualitatif berupa bentuk fisik dari limbah padat RPH Tamangapa dilakukan melalui pengamatan secara visual yang meliputi warna dan tekstur limbah. Kandungan C, rasio C/N dan bahan organik paling tinggi terdapat pada serbuk gergaji, Kandungan N dan P tertinggi terdapat pada isi rumen, sedangkan kandungan K paling tinggi terdapat pada kotoran ternak. Kandungan unsur C, N, P, K dan rasio C/N serta kadar air pada semua bahan baku ternyata memenuhi standar SNI 19­7030­2004 untuk kualitas pupuk kompos, kecuali kandungan bahan organik pada isi rumen dan kotoran ternak masih rendah dan pH yang lebih tinggi. Isi rumen tampak berwarna cokelat muda dengan tekstur yang kasar sedangkan kotoran ternak berwarna cokelat kehitaman, tekstur lebih halus dan berbentuk gumpalan menyerupai tanah.
Produksi Biogas Limbah Isi Rumen Sapi Asal Rumah Pemotongan Hewan (RPH) ., Ramli; ., Hartono
bionature Vol 16, No 2 (2015): Oktober
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (186.273 KB) | DOI: 10.35580/bionature.v16i2.2467

Abstract

Saat ini pemenuhan kebutuhan energi dalam negeri perlu diarahkan kepada diversifikasi sumber-sumber energi selain minyak bumi, salah satunya dengan program “waste to energy” dimana biogas adalah salah satu contohnya. Bahan yang bisa digunakan salah satunya adalah isi rumen sapi yang merupakan limbah Rumah Pemotongan Hewan (RPH) berupa rumput yang belum terfermentasi dan tercerna sepenuhnya oleh hewan. Di dalamnya terkandung bakteri metanogenik dan selulolitik sehingga bahan ini sangat efisien dalam membuat biogas. Penelitian ini bertujuan untuk mengetahui potensi penggunaan limbah isi rumen sapi sebagai bahan baku utama pembuatan biogas. jenis penelitian berupa pre experimental design. Jenis data yang digunakan adalah data primer yang diperoleh dari hasil pengukuran terhadap kuantitas biogas, temperatur dan kadar keasaman pada limbah isi rumen sapi. Berdasarkan data hasil penelitian, Rata-rata produksi biogas selama 21 hari sebanyak 21,57 ml dengan suhu rerata berkisar antara 29oC-30oC. Adapun kadar keasaman digester berada dalam kondisi normal. Limbah isi rumen sapi cukup potensial untuk dijadikan sebagai bahan baku utama produksi biogas.
16s rRNA Sequence Analysis and Ammonium Excretion Ability of Nitrogen Fixing Bacteria Isolated from Mineral Acid Soil H. Hartono; Jaka Widada; Siti Kabirun
Indonesian Journal of Biotechnology Vol 14, No 2 (2009)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (283.87 KB) | DOI: 10.22146/ijbiotech.7812

Abstract

Nitrogen fixing bacteria defined as bacteria which is capable to transform free nitrogen molecules into ammonium v (PCR). Nitrogenase activity of these selected isolates was measured using Acetylene Reduction Assay (ARA). The ability of these selected isolates in ammonium excretion was qualitatively and quantitavely measured using Nessler reagent and spectrophotometry method respectively. Taxonomic position of the selected bacteria were determined based on their 16S rRNA sequence analysis. Genetic diversity analysis of these 15 isolates of nitrogen fixing bacteria yield eight selected bacteria for subsequent analysis. Sequence of nifH gene from all of these selected bacteria were successfully amplified. Nitrogenase assay of these selected bacteria revealed 6 isolates with high nitrogen fixation capasity namely GMA3, GMA5, GMA6, GMA9, GMA12 AND GMA 13.</div><div>Ammonium excretion analysis revealed 4 isolates which have remarkable ability of producing high level of ammonium namely GMA1, GMA3, GMA6, and GMA9. The 16S rRNA sequence analysis shown that isolates GMA3, GMA5, GMA11 and GMA12 had a close relationship with Brevibacillus formosus strain DSM 9885T, Flexibacter canadensis strain ISSDS-428, Rhizobium tropici strain rif 200849, and Azotobacter tropicalis strain RBS. Respectively, isolate GMA1 and GMA13 had a close relationship with Sthenotropphomonas sp. Strain MFC-C, while isolate GMA6 and GMA9 had a close relationship to Azotobacter vinelandii strain ISSDS-428.</div>', 'string'),(105, 'en_US', 'subject', 'nitrogen fixing bacteria, ammonium excretion, identification', 'string'),(105, 'en_US', 'sponsor', '', 'string'),(107, 'en_US', 'title', 'Effect of Probiotic Lactobacillus sp. Dad13 on Humoral Immune Response of Balb/C Mice Infected with Salmonella typhimurium', 'string'),(107, 'en_US', 'abstract', 'An indigenous strain of lactic acid bacterium (LAB) identified as Lactobacillus spp. Dad13 (Dad13), isolated from traditional fermented buffalo milk, was found to be potential as probiotic. The aim of this research was to study the effect of probiotic Dad13 on humoral immune response of Balb/C mice infected with Salmonella typhimurium. Thespecific objective was to find out the effect of different Dad13 consumption time (before and along with infection of S. typhimurium) on the humoral immune response of Balb/C mice. The experiment was conducted by in vivo trial on 20 males of Balb/C mice, age of 6-8 weeks, fed with AIN-93 standard diet. The mice were assigned into 4 groups. Each group received the following treatments, ie. Dad13 only, Dad13 before infection, Dad13 along with infection and Salmonella infection only. A volume of 100 &mu;l Dad13 cell suspensions (1010 CFU/ml) were given by oral forced feeding daily for a week, at week 3 for group before infection and at week 4 for group of Dad13 only and Dad13 along with infection. Salmonella infection (109 CFU/ml) was given once orally at week 4 to all groups except group treated with Dad13 only. The humoral immune response of Balb/C mice was detected 2 weeks after infection by measuring the titers of IgG and IgA specific from serum and mucosal intestinal liquid samples using Enzyme-linked Immunosorbent Assay (ELISA) method. The result indicated that humoral immune response of Balb/C mice consuming Dad13 before and along with Salmonella infection were significantly different (p<0.05). Dad13 consumption along with Salmonella infection increased circulated IgG and IgA as well as secretory IgA. It can be concluded that Dad13 probiotic feeding along with infection increased humoral immune response more significantly compared to that before infection.
Komposisi Kimia Pupuk Kompos dari Limbah Padat Rumah Potong Hewan Tamangapa Kota Makassar Hartono Hartono; Surahman Nur; Oslan Jumadi
bionature Vol 23, No 1 (2022): April
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v23i1.34365

Abstract

Abstract. Slaughterhouses (RPH) produce large amounts of solid waste every day and have not been utilized optimally. RPH solid waste has great potential to be used as plant fertilizer through the composting process. Composting is expected to change the organic material composition of the abattoir solid waste to be more ideal to support plant growth. This study aims to determine the chemical composition of compost made from waste at RPH Tamangapa Makassar City before and after the composting process. Composting from solid waste at Tamangapa RPH Makassar was carried out using the main ingredients, namely rumen contents (treatment I) and rumen contents mixed with livestock manure in a ratio of 1:1 (Treatment II). The composting process was carried out in a compost box with a size of 40x40x60 cm with a passive aeration system for 4 weeks with three replications. Quantitative data tested in this study included the content of N-total (%), C-Organic (%), P-total (%), K-total (%), C/N ratio, organic matter (%), water content (%) and compost reduction (%). The results showed that the content of elements C, N, P, K, C/N ratio, organic matter, and water content before composting in both treatment I and treatment II were generally still low and almost the same as the raw materials. After the composting process took place, the content of C, P, K, C/N ratios generally showed an increase, while the N content was relatively constant. The results of the analysis showed that there was no significant difference between the chemical composition of the compost in the treatment and both before and after the composting process.Keywords: RPH solid waste, composting process, chemical composition of compost, C/N ratio.
Seleksi dan Karakterisasi Bakteri Penambat Nitrogen Non Simbiotik Pengekskresi Amonium Pada Tanah Pertanaman Jagung (Zea mays L.) dan Padi (Oryza sativa L. ) Asal Kabupaten Barru, Sulawesi Selatan, Indonesia . Hartono; Oslan Jumadi
Sainsmat : Jurnal Ilmiah Ilmu Pengetahuan Alam Vol 3, No 2 (2014): September
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/sainsmat3211222014

Abstract

Penelitian ini merupakan penelitian deskriptif yang bertujuan untuk menyeleksi (screening) bakteri penambat nitrogen non simbiotik yang diisolasi dari rhizosfer tanaman jagung (Zea mays L.) dan padi (Oryza sativa L.) yang memiliki kemampuan mengekskresikan amonium. Sampel diambil dari daerah Mangkoso, Kabupaten Barru, Provensi Sulawesi Selatan pada daerah rhizosfer tanaman. Isolasi bakteri penambat nitrogen dilakukan dengan menggunakan medium pertumbuhan bebas nitrogen yaitu medium Burk’s dan Ashby. Koloni bakteri yang tumbuh pada medium pertumbuhan selanjutnya dimurnikan dan diuji kemampuan ekskresi amoniumnya dengan menggunakan metode spectrophotometry. Isolat bakteri yang memiliki kemampuan mengekskresikan amonium dikarakterisasi secara morfologi dan fisiologis. Hasil penelitian menunjukkan terdapat 20 isolat bakteri yang mampu tumbuh pada medium bebas nitrogen, namun setelah dilakukan uji ekskresi amonium hanya diperoleh 9 isolat yang memiliki kemampuan mengekskresikan amonium dari masing-masing medium pertumbuhan. Isolat bakteri dan konsentrasi amonium yang diekskresikan masing-masing adalah ABJ211 (179 µg/L), ABP213 (269 µg/L), ABP211 (162 µg/L), ABP131 (254 µg/L), ABP242 (104 µg/L), BBJ221 (263 µg/L), BBJ222 (272 µg/L), BBP222 (269 µg/L) dan BBP214 (257 µg/L). Kesembilan isolat tersebut memiliki karakteristik morfologi dan fisiologis yang bervariasi terutama pada sifat struktur dinding sel (gram), kemampuan hidrolisis pati, gelatin dan kasein, sementara kemampuan dalam mereduksi nitrat dan H2O2 (reaksi katalase positif) serta fermentasi glukosa menunjukkan karakter yang seragam.Kata kunci: Bakteri Penambat Nitrogen, Non Simbiotik, Ekskresi Amonium, Karakterisasi Morfologi dan Fisiologis
Hubungan Pengetahuan Gizi dan Tingkat Kecukupan Zat Gizi dengan Status Gizi Peserta Didik Kelas XI MIPA di SMAN 2 Maros Wahdaniyyah Wahdaniyyah; A. Mushawwir Taiyeb; Hartono Hartono
Biology Teaching and Learning Vol 5, No 2 (2022): Desember
Publisher : Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/btl.v5i2.39563

Abstract

This study aims to determine the relationship between nutritional knowledge and nutritional adequacy levels with the nutritional status of students in class XI MIPA at SMAN 2 Maros. This research is a descriptive correlational research. The research population is all students of class XI MIPA at SMAN 2 Maros as many as 76 people. The sampling technique is a total sampling technique by making all members of the population a sample. Data were analyzed descriptively and inferentially using partial correlation test and multiple correlation test. The results of this study indicate that there is a relationship between nutritional knowledge and the level of nutritional adequacy with the nutritional status of students with a significance value of 0.000 <0.05. The evenness of the relationship is in the "strong" category based on the correlation value (R) of 0.610. The contribution of nutritional knowledge and nutritional adequacy level to nutritional status based on the coefficient of determination (R2) is 37.3% while the remaining 63.7% is the influence of other factors.
Pelatihan Budidaya Jamur Tiram pada Anggota MGMP Biologi SMA Kabupaten Takalar Hartono Hartono; Oslan Jumadi; Andi Rahmat Saleh; Siti Saenab; Marsito Marsito
Seminar Nasional Pengabdian Kepada Masyarakat SEMINAR NASIONAL 2022:PROSIDING EDISI 6
Publisher : Seminar Nasional Pengabdian Kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Abstrak. Mitra Program Kemitraan Masyarakat (PKM) ini adalah Musyawarah Guru Mata Pelajaran (MGMP) Biologi SMA Kabupaten Takalar. Masalah yang dihadapi oleh anggota kelompok mitra adalah masih terbatasnya pengetahuan dan keterampilan guru-guru Biologi dalam mengajarkan dan mempraktekkan teknik budidaya jamur tiram kepada siswa. Permasalahan ini menjadi salah satu faktor penghambat bagi guru biologi di Kabupaten Takalar dalam melaksanakan proses belajar mengajar, khususnya pada mata pelajaran pelajaran Prakarya dan Kewirausahaan. Metode yang digunakan dalam kegiatan PKM ini adalah metode ceramah, diskusi, tanya jawab, demonstrasi dan metode praktikum. Hasil yang dicapai menunjukkan bahwa anggota kelompok mitra mendapatkan manfaat langsung dari kegiatan PKM yang dilaksanakan dalam hal peningkatan pengetahuan dan keterampilan mereka dalam membudidayakan jamur tiram khususnya pada aspek pembuatan media pertumbuhan jamur tiram, pembuatan bibit jamur tiram, teknik inokulasi bibit dan pemeliharaan jamur tiram. Peserta juga merasa puas dengan pelayanan yang diberikan oleh tim PKM selama kegiatan pelatihan berlangsung..Kata kunci: Jamur tiram, teknik budidaya, PKM, MGMP Biologi, Kabupaten Takalar
Tinjauan Molekuler dan Epidemiologi Mutasi pada Virus SARS-CoV-2 Hartono Hartono; Yenni Yusuf
bionature Vol 22, No 1 (2021): April
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v22i1.22379

Abstract

Abstract. The SARS-CoV-2 virus which is the cause of the COVID-19 pandemic since the end of 2019 has undergone many mutations that gave rise to several variants of concern (VOC) with higher transmission, virulence, and ability to evade the immune system than the initial variant (wild-type). Until now, there are four variants included in the VOC of the virus, namely alpha, beta, gamma and delta variants. The increased transmission and virulence of these VOCs were associated with mutational changes in the spike protein, which is the structure of the virus that plays a role in binding to host cells. In this article, we conduct a literature review on VOCs from the SARS-CoV-2 virus related to mutations that occur and their impact on the viral binding process. To gain an understanding of the impact of mutations in these variants, we also reviewed the structure of the spike protein and the process of viral entry into host cells. Keywords: viral mutation, variants of concern (VOC), COVID-19, SARS-CoV-2.
Cemaran Bakteri pada Bakso yang Beredar di Kota Makassar Hartono Hartono; Alimuddin Ali; Irma Suryani Idris; Hikmanul Irfiany Daud
Indonesian Journal of Fundamental Sciences Vol 7, No 2 (2021)
Publisher : Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (459.4 KB) | DOI: 10.26858/ijfs.v7i2.26154

Abstract

Daging dan produk olahannya merupakan sumber kebutuhan protein asal hewan yang dibutuhkan dan banyak diminati oleh masyarakat, khususnya di Kota Makassar. Mikroba patogen seringkali mencemari daging dan produk olahannya seperti bakso. Hal ini sangat berbahaya karena dapat menimbulkan penyakit pada manusia. Oleh karena itu peningkatan keamanan pangan terhadap makanan asal hewan dan olahannya mutlak dilakukan untuk mencegah dan menurunkan prevalensi food borne pathogens. Tujuan dari penelitian ini adalah untuk mengetahui kandungan total bakteri pada sampel daging bakso yang dijual di Kota Makassar. Sampel penelitian berupa daging bakso yang diambil dari delapan lokasi yang berbeda di kota Makassar. Metode pengujian yang digunakan dalam penelitian ini mengacu pada Standar Nasional Indonesia (SNI) 2897:2008 dengan menggunakan metode pengujian total plate count (TPC). Data yang diperoleh dianalisis secara kualitatif dengan membandingkan data tersebut dengan standar tingkat cemaran bakteri berdasarkan peraturan BPOM No HK.00.06.1.52.4011 dan SNI 7388:2009. Hasil penelitian menunjukkan bahwa kandungan total bakteri pada 8 sampel bakso yang beredar di Kota Makassar berkisar antara 3,25 x 103 cfu.g-1 sampai 3,09 x 105 cfu.g-1. Kandungan total bakteri tertinggi ditemukan pada sampel BKS-A yaitu 3,09 x 105 cfu.g-1 dan kandungan total bakteri terendah ditemukan pada sampel BKS-D yaitu 3,25 x 103 cfu.g-1. Ada 2 sampel bakso yang yang mengandung jumlah total bakteri diatas batas maksimum cemaran bakteri pada produk olahan daging (1 x 105 cfu.g-1) berdasarkan peraturan BPOM No HK.00.06.1.52.4011 dan SNI 7388:2009 yaitu sampel BKS-A dan sampel BKS-G sedangkan kandungan total bakteri pada sampel BKS-B, BKS-C, BKS-D, BKS-E, dan BKS-F dan masih memenuhi standar tersebut. Hasil ini menunjukkan bahwa keenam sampel tersebut masih layak untuk dikonsumsi.
Validity of Practicum Guide in Molecular Genetics Course for Bachelor's Student of Biology Study Program of Mathematic and Science Faculty in Universitas Negeri Makassar Aulya Novira; Oslan Jumadi; Hartono Hartono; Irma Suryani
Biology Teaching and Learning Vol 6, No 1 (2023): Juni
Publisher : Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/btl.v6i1.46774

Abstract

Abstract. The formulation of the problem in this research is how to develop a valid molecular genetics practicum guide with the aim of testing the validity of the practicum guide in molecular genetics courses for undergraduate biology study program students. This type of research is Research and Development (R&D) with the ADDIE development model which consists of five stages, namely analysis, design, development, implementation and evaluation. The instrument used is a validation sheet filled out by a validator expert. Data was collected through analysis of validation sheets that had been filled in by expert validators and analyzed descriptively in order to obtain data in the form of a validity score. The results showed that practicum guides had an average validity score in the range of 4.5 ≤ Va = 5, in this case it met the very valid criteria.Keywords: ADDIE development model, practicum guide, molecular genetics
Co-Authors . Syafruddin A. Mushawwir Taiyeb Alimuddin Ali Andi Rahmat Saleh Aulya Novira Ayu Andi Indra, Ayu Bakri Zakia, Bakri Cut Muthiadin Halifah Pagarra Hikmanul Irfiany Daud Hilda Karim Idris, Irma Suryani Irma Suryani JAKA WIDADA Liawati ., Liawati Marsito Marsito Oslan Jumadi Oslan Jumadi Oslan Jumadi Ramli . Siti Kabirun Siti Saenab St. Fatmah Hiola Surahman Nur Surahman Nur, Surahman Syamsiah, Syamsiah Wahdaniyyah Wahdaniyyah Yenny Yusuf Yusuf Yenni