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Syarif Irwan Nurdiansyah
Universitas Tanjungpura
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Journal : Journal of The Indonesian Society of Integrated Chemistry

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Fraksinasi dan Potensi Antioksidan dari Ekstrak Protein Kasar dari Spons Haliclona sp Asal Perairan Spermonde Sulawesi Selatan Warsidah Warsidah; Mega Sari Juane Sofiana; Ikha Safitri; Syarif Irwan Nurdiansyah
Journal of The Indonesian Society of Integrated Chemistry Vol. 15 No. 2 (2023): Journal of The Indonesian Society of Integrated Chemistry
Publisher : Pendidikan Kimia FKIP Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/jisic.v15i2.25557

Abstract

Sponge Haliclona Sp is a member of the phylum porifera class Demospongia which is found living in the waters of the spermonde coral reefs of South Sulawesi. Its interaction with various microorganisms that live in the waters causes the formation of various kinds of active compounds and biological activities that are important in the world of health. The purpose of this study was to extract and fractionate protein from the Haliclona Sp sponge from Spermonde waters through confirmation using ammonium sulfate at various antioxidant levels, and to determine the content and activity of the protein in each fraction. The results showed that the protein content and antioxidant activity (IC50) of each fraction were, among others, in fraction 1 (0-30%) of 0.143 mg/L and 78.11 mg/L, fraction 2 (31-50%) 0.045 mg/L and 153.55 mg/L, fraction 3 (51–70%) of 0.020 and 161.12 mg/L, and fraction 4 (71–90%) of 0.112 and 104.71 mg/L, while crude protein of 0.277 mg/L and 115.22 mg/L. Ascorbic acid as a positive antioxidant control in this study resulted in an antioxidant activity of 2.74 mg/L.
Co-Authors . Apriansyah Arie Antasari Kushadiwijayanto Arie Antasari Kushadiwijayanto Ashari, Asri Mulya Bambang Kurniadi Desriani Lestari Edwin Enjella Enjella Fadiah Amalia Hasanah Helena, Shifa Ikha Safitri Ikha Safitri Joko Septian Tri Putra Lucky Hartanti Madi Juna Permalem Ginting Mega Sari Juane Sofiana Rita Kurnia Apindiati Riza Linda Sella Agustina Seri Subarni Setra Kusumardana Sukal Minsas Tahirah Hasan Warsidah Warsidah Warsidah Yasnidar Yasnidar Yuliono, Agus Yusuf Arief Nurrahman Yusuf Arief Nurrahman