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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Berkala Ilmu Kesehatan Kulit dan Kelamin Indonesian Journal of Tropical and Infectious Disease
Linda Astari, Linda
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Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Education and Workshop Improve Healthcare Workers' Knowledge of Laboratory Examination for the Diagnosis of Superficial Dermatomycosis Ervianti, Evy; Astari, Linda; Rahmadewi; Setyaningrum, Trisniartami; Mappamasing, Hasnikmah; Anggraeni, Sylvia; Widia, Yuri; Ngesti Kumalasari, Diah; Axelia, Presstisa Gifta; Hadiwidjaja, Farsha Naufal; Sunarto, Olivia Awwalin; Kathrin Kezia Henry; Sulaksanaswastho Suyoso; Puspowati, Erindah
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol. 35 No. 3 (2023): DECEMBER
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bikk.V35.3.2023.187-192

Abstract

Background: Superficial dermatomycosis is one of the most prevalent skin diseases worldwide. The diagnosis of  superficial dermatomycosis is established clinically, and supported by several examinations, such as microscopic examination, fungal culture, and Wood's lamp examination for certain species. Purpose: This study is to evaluate the improvement of the knowledge of general practitioners and medical analysts after education and workshop. Methods: This cross-sectional observational study involved 70 subjects, consisting of 35 general practitioners and 35 medical analysts from 35 public health centers in Bojonegoro who were willing to participate in this study. The level of knowledge was assessed before and after the health education on laboratory examination for the diagnosis of superficial dermatomycosis. Result: There was a significant difference in mean scores between the pre-test and post-test after health education (p = <0.001). The mean knowledge score of the pre-test before intervention among general practitioners was 36.57 ± 19.088, which increased to 68.00 ± 13.240, and the mean knowledge score before intervention among analysts was 27.14 ± 13.842, which increased to 62.00 ± 12.078 after health education intervention. Conclusion: Following health education, there was an improvement in the healthcare workers's knowledge of laboratory examinations for the diagnosis of superficial dermatomycosis
Mycobacterium leprae Survival Inside Acanthamoeba sp. Isolated from Water Source in Leprosy Endemic Area, Indonesia: Mycobacterium leprae Survival Inside Acanthamoeba sp. Wahyuni, Ratna; Astari, Linda; Iswahyudi, Iswahyudi; Paling, Sepling; Adriaty, Dinar; Kurniawati, Siti; Aulia, Syifa; Rahmatari, Bandaru; Prakoeswa, Cita Rosita; Agusni, Indropo; Izumi, Shinzo
Journal of Tropical Life Science Vol. 14 No. 2 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.02.07

Abstract

Mycobacterium leprae is an acid-fast bacterium that causes leprosy diseases, which remains a problem worldwide. Even though leprosy prevalence in the world has decreased significantly, many endemic pocket areas continue reporting new cases and harbor M. leprae in the environment, including water and soil. The presence of obligate intracellular bacteria-M. leprae in the environment raises a question on how it survives. Free-living amoeba has been proposed as its reservoir host in the environment. The study was conducted to give evidence that M. leprae can survive inside free-living amoeba isolated from water sources of leprosy endemic areas. M. leprae from leprosy patients was cultured together with Acanthamoeba sp. isolated from the water source of the leprosy endemic area. Viability and duplication of M. leprae inside amoeba then observed at day 14 and 28 using reverse transcriptase PCR and qPCR. The results showed that M. leprae survived inside the amoeba until day 28, but no bacterial replication was observed. The study reveals in vitro evidence of viable M. leprae inside free-living amoeba of leprosy endemic area environment.
Co-Authors Afif Nurul Hidayati, Afif Nurul Alarik L., Albertus Alimsardjono, Lindawati Anggraeni, Sylvia Astindari Astindari, Astindari Aulia, Syifa Auliya, Arsya Axelia, Presstisa Gifta Bagus Haryo Kusumaputra, Bagus Haryo Budi Prasetyo Budi Utomo Budiono Budiono Cita Rosita Sigit Prakoeswa Damayanti Damayanti Diah Mira Indramaya DINAR ADRIATY Dwi Murtiastutik Dwiyanti Puspitasari, Dwiyanti Dyah Ratri Anggarini, Dyah Ratri Evy Ervianti Hadiwidjaja, Farsha Naufal Hari Sukanto, Hari Harningtyas, Citra Dwi Hartanto, Felix Indah Purnamasari Indiastuti, Danti Nur INDROPO AGUSNI Irmadita Citrashanty, Irmadita ISWAHYUDI ISWAHYUDI Kathrin Kezia Henry M. Yulianto Listiawan Mappamasing, Hasnikmah Maylita Sari Maylita Sari, Maylita Medhi Denisa Alinda, Medhi Denisa Meita Ardini Pratamasari, Meita Ardini Ngesti Kumalasari, Diah Noviannisa, Farah Ayu Pepy D. Endraswari Pepy Dwi Endraswari, Pepy Dwi Prakoeswa, Cita Rosita Puspowati, Erindah Rahmadewi Rahmatari, Bandaru RATNA WAHYUNI Sepling Paling Sheilaadji, Maria Ulfa SHINZO IZUMI Siti Kurniawati Sulaksanaswastho Suyoso Sunarto, Olivia Awwalin Trisniartami Setyaningrum Widyantari, Septiana Willy Sandhika Yuri Widia, Yuri