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All Journal JURNAL SAINS PERTANIAN EQUATOR Perkebunan dan Lahan Tropika Jurnal Pertanian Agros
Agustina Listiawati
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science

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RESPON PERTUMBUHAN ANGGREK HITAM PADA BERBAGAI KONSENTRASI EKSTRAK BAWANG MERAH DAN BAP PADA MEDIA MS SECARA IN VITRO Angelic Kusuma Nagari; Asnawati Asnawati; Agustina Listiawati
Agros Journal of Agriculture Science Vol 26, No 1 (2024): Januari
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37159/jpa.v26i1.3922

Abstract

One alternative to preserve the existence of black orchid populations in nature is to carry out ex-situ conservation by multiplying them using in vitro culture methods. In vitro propagation requires the right composition of growth media, including the addition of growth regulators to stimulate shoot and root initiation, especially auxin and cytokinin groups at the right concentration. The aim of this research is to obtain the best interaction concentration of shallot extract and BAP for the growth of black orchids on MS media in vitro. The research was carried out at the Biotechnology Laboratory, Faculty of Agriculture, Tanjungpura University, Pontianak, which lasted for 3 months. This research used a factorial completely randomized design (CRD) with 2 factors. The first factor is shallot extract with 3 treatment levels, namely m₁= 15 g/l, m2 = 30 g/l, m3 = 45 g/l. The second factor is BAP with 3 treatment levels, namely b1 = 1 mg/l, b2 = 1.5 mg/l, b3 = 2 mg/l. The variables observed were shoot emergence time (MST), root emergence time (MST), increase in the number of shoots (buds), increase in the number of leaves (strands) and increase in the number of roots (roots). The research results showed that there was no interaction between shallot extract and BAP in influencing the growth of black orchids, of all the growth variables observed. The concentration of shallot extract independently had a significant effect on the variable of increasing the number of leaves, and the shallot extract concentration of 45g/l was an effective concentration, producing 4.55 leaves. Keywords : Black Orchid, BAP, In vitro, Shallot extract  INTISARISalah satu alternatif untuk melestarikan keberadaan populasi anggrek hitam di alam adalah dengan melakukan konservasi  secara ex- situ dengan memperbanyaknya menggunakan metode kultur in vitro.  Perbanyakan in vitro membutuhkan komposisi media tumbuh yang tepat, termasuk penambahan zat pengatur tumbuh untuk merangsang inisiasi tunas dan akar, terutama dari kelompok auksin dan sitokinin dengan konsentrasi yang tepat. Tujuan dari penelitian ini yaitu mendapatkan konsentrasi interaksi ekstrak bawang merah dan BAP yang terbaik untuk pertumbuhan anggrek hitam pada media MS secara in vitro. Penelitian telah dilaksanakan di Labolatorium Bioteknologi Fakultas Pertanian, Universitas Tanjungpura Pontianak yang berlangsung selama 3 bulan. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) Faktorial dengan 2 faktor. Faktor pertama yaitu ekstrak bawang merah dengan 3 taraf perlakuan yaitu m₁= 15 g/l, m2 = 30 g/l, m3 = 45 g/l. Faktor kedua yaitu BAP dengan 3 taraf perlakuan  yaitu b1 = 1 mg/l, b2 = 1,5 mg/l, b3 = 2 mg/l. Variabel yang diamati yaitu waktu muncul tunas (MST), waktu munculnya akar (MST), pertambahan jumlah tunas (tunas), pertambahan jumlah daun (helai) dan pertambahan jumlah akar (akar). Hasil penelitian menunjukan bahwa tidak terjadi interaksi antara ekstrak bawang merah dan BAP dalam mempengaruhi pertunbuhan anggrek hitam, dari semua variable pertumbuhan yang diamati. Konsentrasi ekstrak bawang merah secara mandiri berpengaruh nyata terhadap variabel pertambahan jumlah daun, dan konsentrasi ekstrak bawang merah 45g/l merupakan konsentrasi yang efektif dengan menghasilkan 4,55 helai. Kata Kunci: Anggrek hitam, Ekstrak Bawang Merah, BAP, In vitro
PENGARUH PUPUK KANDANG SAPI DAN BERBAGAI JARAK TANAM TERHADAP PERTUMBUHAN DAN HASIL BAWANG DAUN PADA TANAH GAMBUT Tiurida Marbun; Agustina Listiawati; Dwi Zulfita
Agros Journal of Agriculture Science Vol 26, No 2 (2024): Edisi Juli
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37159/jpa.v26i2.4766

Abstract

Scallions ( Allium fistulosum L.) are a horticultural crop commodity that is worthy of intensive development in Indonesia, especially in Pontianak, West Kalimantan Province. Peat soil has great potential for the development and cultivation of spring onions. Efforts that can be made to overcome the very low availability of nutrients are cow manure. One cultivation technique that can be used to increase leek production is by adjusting plant spacing. This research was carried out on research land located on Jalan Sungai Raya Dalam, Alam Mulia Housing Complex starting on 15 September – 2 November 2023. This research aims to obtain the best dose of cow manure for the growth and yield of leek plants on peat soil and to obtain the best planting distance for the growth and yield of leek plants on peat soil. This research was carried out using a field experimental method with a Split Plot Design pattern , namely: The first factor is the distance between the plants for the main plot: j 1 = 20 cm x 20 cm, j 2 = 20 cm x 25 cm and j 3 = 20 cm x 30 cm. The second factor is cow manure as a sub plot: p 1 = 10 tonnes/ha, p 2 = 20 tonnes/ha and p 3 = 30 tonnes/ha. The observation variables consist of: Root Volume, Plant Dry Weight, Number of Leaves/clump, Plant Height, Number of Saplings/clump and Fresh Weight of the plant. From research that has been carried out, the interaction of using a planting distance of 20 x 25 cm and 10 tonnes/ha of cow manure shows the best growth and yield of leek plants on peat soil.
Co-Authors Acinake Acinake Adriantono Adriantono Adrianus Aping Akhira Nurfansyah Zed Angelic Kusuma Nagari Anik Dwi Aryanti ARTI PURNAMA SARI Asnawati . Asnawati Asnawati Basuni Basuni Darussalam - Dian Asmarawati Dini Anggorowati dwi zulfita eni kusmawati eni septiarni Erlangga Tungga Dewa Evi Gusmayanti F.X Widadi Padmarsari Gustami Gustami Gustian Gustian Inpurwanto Inpurwanto Ishak Dianisius KASIM PURNAMA YUSUF Kurnilah Ary Fitri Maulidi Maulidi Meylani Dwi Jayanti mita elfini Murti Lestari NORMALA NORMALA Nur Arifin Nurbaiti R Rafi Adista Putra Rahmidiyani Rahmidiyani Rini Susana RISKA MASYURA Sahwaldi Sahwaldi Setia Budi SRI RAHAYU Surachman Surachman Tantri Palupi Tiurida Marbun Tri Indra Wijaya Ummu Mardhiyyaturrosyidah WALKIS WALKIS Warganda Warganda Wasi’an Wasi’an YERIKHO JIMMY KURNIAWAN Yuspiatuti Yuspiatuti