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Isolasi Jamur Endofitik Rumput Mutiara (Hedyotis corymbosa (L.) Lamk.) dan Analisis Potensi Sebagai Antimikroba Pratika Viogenta; Siti Nurjanah; Yuli Wahyu Tri Mulyani
Jurnal Pharmascience Vol 7, No 1 (2020): Jurnal Pharmascience
Publisher : Program Studi Farmasi FMIPA Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jps.v7i1.8076

ABSTRAK Jamur endofit merupakan jamur yang hidup di dalam jaringan atau organ tanaman yang tidak bersifat patogen. Jamur ini dapat menghasilkan beberapa zat yang sama seperti tanamannya. Rumput mutiara (Hedyotis corymbosa (L.) Lamk) merupakan jenis rumput liar yang biasa hidup di tanah lembab. Tujuan penelitian ini adalah untuk mengetahui keanekaragaman mikroba endofit rumput mutiara. Metode dari penelitian ini meliputi isolasi jamur endofit dari batang, daun, akar, bunga rumput mutiara, identifikasi jamur yang berhasil diisolasi, pengukuran diameter laju pertumbuhan jamur endofit dan uji daya hambat antimikroba dari jamur endofit. Hasil penelitian didapatkan tujuh jenis jamur endofit yang berhasil diisolasi dari rumput mutiara (batang, daun, akar dan bunga). Setelah diindentifikasi ketujuh jenis kapang, yaitu 2 jenis dari genus Aspergillus yaitu A. niger dan A. parasiticus yang berhasil diisolasi dari akar, batang, daun rumput mutiara, Penicillium berasal dari akar rumput mutiara, Geotrchihum dari batang, Chrysosporium dari daun, Rhizoctonia dan Phytophthora dari bunga. Pada ketujuh jamur endofit ini fase lag terjadi hari pertama dan fase eksponensial terlihat pada hari kedua sampai hari ketujuh. Aspergillus niger memiliki daya hambat terhadap E.coli, St. aureus, Sh. disentriae,dan P.aeruginosa masing-masing 9.95mm, 8.96mm, 10.51mm, dan 9.26mm. Chrysosporium dan Phytophthora mengahambat C. albicans sebesar 11.3mm dan 3.63mm. Kata Kunci: Rumput mutiara, jamur endofit, antimikroba. ABSTRACT Endophytic fungi are fungi that live in plant tissues or organs that are not pathogenic. This fungus can produce some of the same substances as the plant. Pearl grass (Hedyotis corymbosa (L.) Lamk) is a type of weed that normally lives in moist soil. The purpose of this study was to determine the diversity of endophytic pearl grass microbes. The methods of this study include the isolation of endophytic fungi from stems, leaves, roots, and flowers of pearl grass, identification, measurement of the diameter of endophytic fungi growth rate and antimicrobial inhibition test of endophytic fungi. The results showed seven types of endophytic fungi that were isolated from pearl grass. After identifying the seven types of molds, namely 2 types of the genus Aspergillus namely A.niger and A.parasiticus which were successfully isolated from roots, stems, pearl grass leaves, Penicillium derived from pearl grass roots, Geotrchihum from the stems, Chrysosporium from leaves, Rhizoctonia and Phytophthora from flowers. In these seven endophytic fungi the lag phase occurs the first day and the exponential phase is seen on the second day to the seventh day. Aspergillus niger has inhibitory properties against E.coli, St.aureus, Sh.disentriae, and P.aeruginosa 9.95mm, 8.96mm, 10.51mm and 9.26mm, respectively. Chrysosporium and Phytophthora inhibit C. albicans by 11.3mm and 3.63mm. Keywords: Pearl grass, endophytic fungus, antimicrobial

EKSTRAK DAUN KATUK (Sauropus androgynus (L) Merr) SEBAGAI ANTIBAKTERI TERHADAP Propionibacterium acnes dan Staphylococcus epidermidis Yuli Wahyu Tri Mulyani; Dadan Hidayat; Isbiyantoro Isbiantoro; Yeni Fatimah
JFL : Jurnal Farmasi Lampung Vol. 6 No. 2 (2017): JFL: Jurnal Farmasi Lampung
Publisher : Fakultas MIPA Universitas Tulang Bawang Lampung

Katuk is one of the most common plant species in Indonesia which is known by the community to treat the disease, especially the leaf part can be used as a milk mat. katuk leaf (Sauropus androgynus (L) Merr) also contains saponin, flavonoid and tannin Inhibits the growth of bacteria one of the bacteria that cause acne bacteria P. acnes and S. epidermidis. The aim of this research is to know the antibacterial inhibition effect of katuk leaf extract to growth of P. acnes and S. epidermidis using Cup-plate or pit diffusion method with concentration 20%, 40%, 60%, 80% and 100%. Clindamycin is used as a positive control and aquades as a negative control. The leaves of katuk extracted with 70% ethanol solvent showed no antibacterial activity against P. acnes bacteria while against S. epidermidis bacteria showed antibacterial activity. The highest antibacterial activity at 100% concentration with 18.17 mm zone diameter belong to the category of inhibitory resistance of medium growth and the lowest concentration at concentration of 40% with 16.68 mm inhibitory zone diameter belong to the weak growth inhibitory response category. The minimum inhibitory concentrations produced in S. epidermidis bacteria were 39% - 36%. Keywords : Sauropus androgynus, Propionibacterium acnes, Staphilococcus epidermidis, Minimal Inhibitory Concentration.

FRAKSI ETANOL RIMPANG TEMU PUTIH (Curcuma zedoaria (Christm.) Rosc.) SEBAGAI ANTIFUNGI TERHADAP Candida albicans dan Trichophyton rubrum Yuli Wahyu Trimulyani; Nurma Suri; Niken Dwi Astarina
JFL : Jurnal Farmasi Lampung Vol. 7 No. 2 (2018): JFL: Jurnal Farmasi Lampung
Publisher : Fakultas MIPA Universitas Tulang Bawang Lampung

Traditional medicine is used as natural because it has small effects and more economical than the synthetic drugs. One example of the plants used as a Traditional medicine are Curcuma zedoaria. The study to examine the activity fraction of Curcuma zedoaria rhizome ethanol extract agains Candida albicans and Trichophyton rubrum. The simplisia of Curcuma zedoaria rhizome was extracted with ethanol 70% using a maceration method. Thick extracts were then fractionated using saveral solven such as n-heksane, chloroform and ethanol. The testing method used disc antifungi activity with the concentration of 6.25%, 12.5%, 25%, 50%, 75%, ketokonazol as the positive control and aquades as the resolvent. The test compounds Thin Layer Chromatography (TLC) method with eluen chloroform: methanol: water (2:3:5(v/v/v)) and phase uses G60F254 had been done. The result showed that Curcuma zedoaria rhizome significantly inhibit of C. albicans and T. rubrum the best inhibit zone diameter that are concentration of 75% for both of them with a diameter 8.98 mm in C. albicans and 11.66 mm in T. rubrum. Minimum Inhibitory Concentration (MIC) C. albicans is obtained on concentration of 6.25% and T. rubrum is concentration on of 12.5%. The TLC results showed a compound of flavonoids (Rf 0.88), saponins (Rf 0.74), tannins (Rf 0.86). Minimum Inhibitory Concentration (MIC) in C. albicans and T. rubrum are fungistatik and Minimum Bactericidal Concentration (MBC) are not fungisid for both of them. The ethanol fraction of Curcuma zedoaria rhizome has an activity as an antifungal against C. albicans and T. Rubrum with flavonoids, tannins and saponins. Keywords: Antifungi, Candida albicans, Curcuma zedoaria, Fungistatic, Trichophyton rubrum.

FRAKSI ETANOL EKSTRAK KULIT DURIAN (Durio zibethinus L.) SEBAGAI ANTIFUNGI TERHADAP Trichophyton mentagrophytes DAN Candida albicans Yuli Wahyu Tri Mulyani; Subur Widodo; Lisa Selviani
JFL : Jurnal Farmasi Lampung Vol. 8 No. 1 (2019): JFL: Jurnal Farmasi Lampung
Publisher : Fakultas MIPA Universitas Tulang Bawang Lampung

Durian is a fruit that is in demand by the community, but during durian season environmental problems arise due to waste from skin of durian. The aimed of the study was to prove the antifungal activity of the ethanol fraction of durian skin against Trichophyton mentagrophytes and Candida albicans and to determine the active active compounds inhibiting T. mentagrophytes and C. albicans fungi. Durian skin was extracted by maceration method, then fractionated used ethanol, chloroform, and n-hexane solvents. Ethanol fraction was tested on T. mentagrophytes and C. albicans fungi used the disc method with a concentration of 10%, 20%, 40%, 60%, 80%, 100%, distilled water as a negative control, ketoconazole as a positive control. The best inhibition zone is fungi T. mentagrophytes concentration 100% ( 9.23 mm) negative control (0 mm), positive control (32.96 mm), fungi C. albicans the best inhibition zone is concentration of 100% (8.30 mm), positive control (17.17 mm) negative controls (0 mm).The ethanol fraction was screened by the Thin Layer Chromatography method and positively contained flavonoids (rf 0.7), tannins (rf 0.58), saponins (rf 0.76) and alkaloids (rf 0.64). Minimum Inhibitory Concentrations of T. mentagrophytes were 7% and C. albicans were 8%. The most effective active compound growht of T. mentagrophytes is tannins (rf 0.7) and for C. albicans is flavonoid (rf 0.6). The ethanol fraction of durian bark extract has antifungal activity against T. mentagrophytes and C. albicans. Keywords: Antifungi, Candida albicans, ethanol fraction skin of durian, Trichophytonmentagrophytes

FRAKSI ETANOL, KLOROFORM, DAN N-HEKSAN BUNGA KAMBOJA PUTIH (Plumeria acuminata L.) SEBAGAI ANTIBAKTERI TERHADAP Escherichia coli dan Staphylococcus aureus DENGANBIOAUTOGRAFI Yuli Wahyu Trimulyani
JFL : Jurnal Farmasi Lampung Vol. 8 No. 2 (2019): JFL: Jurnal Farmasi Lampung
Publisher : Fakultas MIPA Universitas Tulang Bawang Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37090/jfl.v8i2.147

Frangipani flowers (Plumeria acuminata L.) Flower is one of the plants that has antibacterial properties. Compounds that act as antibacterial are flavonoids and tannins. The purpose of this study was to prove the antibacterial activity of ethanol, chloroform, and n-hexane fractions of Frangipani flowers (Plumeria acuminata L.) flower as antibacterial against E. coli and S. aureus with Bioautography.The process of extracting plumeria acuminata L. flowers was carried out by maceration method using 70% ethanol. The extract was continued with the fractionation process with ethanol, n-hexane and chloroform solvents. Antibacterial activity testing using the well method with concentrations of 20%, 40%, 60%, 80%, 100%, positive chloramphenicol control, and negative aquadest control. The compound content test used the Thin Layer Chromatography (TLC) method with the mobile phase of chloroform: methanol: water (2:5:3) (v/v).Bioautography test using the contact method, namely the elution TLC plate, was placed on the NA medium containing a bacterial suspension for 3 hours. The biggest antibacterial test results of ethanol fraction of plumeria acuminata L. flowers were at a concentration of 100% with a diameter of inhibition zone of 13.55 mm in E. coli while 14.15 mm S. aureus. The results of TLC showed the presence of flavonoids with a price of Rf 0.70 and tannin at a price of Rf 0.68. Bioautography results showed a inhibition zone with a price of Rf 0.70 in E. coli and S. aureus which are flavonoid compounds. Based on the results of the research that has been done it can be concluded that the ethanol fraction of white frangipani flowers has antibacterial activity against E.coli and S. aureus, while the chloroform fraction and n-hexane fraction do not have antibacterial activity. The active compounds that act as antibacterial are flavonoids and tannin, but the ones that provide antibacterial effects are flavonoids with an Rf value of 0.70. Keywords: Bioautography,Escherichiacoli, Frangipani flowers, Staphylococcusaureus, TLC

FRAKSI ETANOL EKSTRAK KULIT PISANG KEPOK KUNING ( Musa balbisiana) TERHADAP BAKTERI Staphylococcus epidermidis, Staphylococcus aureus, dan Propionibacterium acnes Yuli Wahyu Tri Mulyani; Akhmad Rokiban; Galih Cipto Mahendra
JFL : Jurnal Farmasi Lampung Vol. 10 No. 1 (2021): JFL : Jurnal Farmasi Lampung
Publisher : Program Studi Farmasi Universitas Tulang Bawang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37090/jfl.v10i1.492

Yellow kepok banana peel (Musa balbisiana) contains antibacterial compounds against S. epidermidis, S. aureus and P.acne. This study aims to proved the antibacterial activity of the yellow kepok banana peel extract fractionated using ethanol as a solvent against S.epidermidis, S.aureus and P.acne. The yellow kepok banana peel extraction process was carried out by maceration method with 70% ethanol followed by fractionation to obtain ethanol fraction. Antibacterial activity testing using nutrient agar (NA) media with wells method with a concentration of 25,000 ppm, 50,000 ppm, 100,000 ppm, aquadest as a negative control and clindamycin as a positive control. The phytochemical test of the ethanol fraction showed the content of alkaloids, flavonoids, saponins, tannins and quinones. The results of the inhibition zone diameter of the ethanol fraction at a concentration of 100,000 ppm against S. epidermidis (11.87 mm), S. aureus (12.04 mm) and P. acne (11.35 mm). The test is to determined the minimum inhibitory concentration (MIC) the minimum inhibitory concentration value of the ethanol fraction of S. epidermidis, S. aureus is 20,000 ppm and for P. acne is 17,500 ppm. The conclusion of this studied was that the ethanol fraction of kepok yellow banana peel extract has inhibitory and bacteriostatic properties against S. epidermidis, S. aureus and P.acne bacteria.Keywords: alkaloids,Ant ibacter ial , flavonoids , Fractionated, Musa balbisiana

Activity Test of Durian Peels Essential Oil (Durio zibethinus) as an Antipediculosis Against Pediculus humanus capitis Linn. (Anoplura: Pediculidae) Yuli Wahyu Tri Mulyani; Siti Nurjanah; Samsuar Samsuar; Laila Susanti
JPSCR: Journal of Pharmaceutical Science and Clinical Research Vol 6, No 3 (2021)
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jpscr.v6i3.49900

Pediculosis is a disease caused by infestation of head lice (Pediculus humanus capitis) which is a blood-sucking obligate ectoparasite. The essential oil of durian fruit peel has active compounds that have the potential as vegetable pediculosides that can kill head lice. This study aimed to find the best concentration of essential oil from durian fruit peels that can kill head lice. The research design used with an essential oil concentration of 100%, 75%, 50%, 25%, distilled water as negative control and permethrin 1% as positive control, three repetitions and each concentration consisting of 10 flea tails. The parameters observed were the condition of lice morphologically based on the activity of limb movement, antenna movement and digestion, then the calculation of mortality was observed based on the time of the test. The results showed that the concentration of durian peel essential oil as an antipediculosis had a significant effect on the time of death of head lice, limb movement and digestion. The test result showed that the concentration is 100%, has a death time of 12 minutes 32 seconds, 75% concentration was 16 minutes 28 seconds, 50% concentration was 21 minutes 11 seconds, 25% concentration was 32 minutes 23 seconds, negative control was 61 minutes 15 seconds. The fastest time of death in positive control for chemical pediculosides was 10 minutes 15 seconds. The conclusion in this study was that essential oils the dominan one is 1,5-naphthyridin-2-amine were proven to kill head lice and the best concentration was close to positive control, namely at a concentration of 100%.

PENDAMPINGAN PENGGUNAAN RAMUAN HERBAL UNTUK TELUR ASIN RENDAH KOLESTEROL SEBAGAI UPAYA PENINGKATAN PENDAPATAN “AMEER DUCK” Yuli Wahyu Tri Mulyani; Samsuar; Novita Tri Wahyuni; Annisa Mulia Anasis
Jurnal Media Pengabdian Kepada Masyarakat Vol. 1 No. 2 (2022): JM-PKM
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat

Teknologi pengolahan telur merupakan usaha untuk mengawetkan, memperpanjang daya simpan, dan mencegah penurunan kualitas telur. Pengolahan telur dapat meningkatkan pendapatan keluarga karena dapat dilakukan dalam skala rumah tangga dan dapat menjadi usaha bagi masyarakat yang hidup dipedesaan. Salah satu cara yang dapat dikembangkan adalah menerapkan teknologi pengolahan telur asin. Kandungan garam dan lemak yang tinggi di dalam telur asinÂ dapat meningkatkan kolesterol dalam darah, menyebabkan hipertensi, sehingga meningkatkan risiko terkena penyakit jantung, stroke, dan penyakit ginjal sehingga konsumsi untuk para orang tua dan lansia perlu dibatasi. Upaya mengatasi hal tersebut perlu upaya inovasi dalam pembuatan telur asin dengan menggunakan ramuan herbal diantaranya menggunakan kayu secang, daun mahkota dewa, daun salam, daun jambu biji dan sereh yang sudah terbukti secara ilmiah dapat menurunkan kadar lemak dan kolesterol dalam darah. Tujuan pengabdian ini adalah memproduksi telur asin herbal yang rendah kolesterol, sehingga telur asin yang diproduksi lebih sehat dengan memanfaatkan tanaman-tanaman disekitar kita yang memiliki potensi sebagai penurun kolesterol serta menginspirasi para pengusaha telur asin untuk berinovasi agar produk yang dihasilkan dapatÂ disenangi konsumen. Solusi yang ditawarkan melalui pembinaan dan pelatihan untuk membuat diversifikasi produk sehingga mitra dapat menghasilkan produk yang lebih banyak macamnya melalui sentuhan teknologi yang aman dan praktis. Diversifikasi produk dilakukan dengan memanfaatkan tanaman herbal antara lain kayu secang, daun mahkota dewa, daun salam, daun jambu biji dan sereh. Kesimpulan dalam pengabdian ini adalah adanya inovasi pembuatan telur asin herbal mampu meningkatakan pendapatan dan kesehatan masyarakat.

SOSIALISASI PENGOLAHAN TELUR ASIN AYAM RAS DI DESA MARGO LESTARI, KECAMATAN JATI AGUNG KABUPATEN LAMPUNG SELATAN Teguh Rafian; Miki Suhadi; Yuli Wahyu Tri Mulyani; Elia Agustiana; Kusuma Adhianto; Dian Kurniawati
Jurnal Media Pengabdian Kepada Masyarakat Vol. 2 No. 1 (2023): JM-PKM
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37090/jmpkm.v2i1.922

Margo Lestari Village is a village with potential as a laying hen farming center. This can be seen from the people who raise laying hens. However, the high egg production in the village is not matched by egg sales or marketing. This results in the production of eggs that can rot before being utilized or consumed because eggs have a fairly short shelf life. Thus, it is hoped that this service activity can increase the income of farmers through improving the quality of salted egg products from laying hen eggs in Margo Lestari Village. This counseling activity uses the lecture method and question and answer section. Extension activities were carried out on Wednesday, October 26, 2022 at 09.00 to 12.30 WIB at the Margo Lestari Village Hall, Jati Agung District, South Lampung Regency. The socialization was attended by 20 people, most of whom were breeders. After carrying out community service activities, it can be concluded that there are several things obtained by farmers as participants, namely increasing knowledge related to processing poultry farm products into salted eggs to increase the selling price and shelf life of production eggs in an effort to increase the economic value of farmers. Keywords: Processed Livestock Products, Economic Value, Egg Storability

SOSIALISASI PENGOLAHAN TELUR ASIN AYAM RAS DI DESA MARGO LESTARI, KECAMATAN JATI AGUNG KABUPATEN LAMPUNG SELATAN Teguh Rafian; Miki Suhadi; Yuli Wahyu Tri Mulyani; Elia Agustiana; Kusuma Adhianto; Dian Kurniawati
Jurnal Media Pengabdian Kepada Masyarakat Vol. 2 No. 1 (2023): JM-PKM
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37090/jmpkm.v2i1.922

Margo Lestari Village is a village with potential as a laying hen farming center. This can be seen from the people who raise laying hens. However, the high egg production in the village is not matched by egg sales or marketing. This results in the production of eggs that can rot before being utilized or consumed because eggs have a fairly short shelf life. Thus, it is hoped that this service activity can increase the income of farmers through improving the quality of salted egg products from laying hen eggs in Margo Lestari Village. This counseling activity uses the lecture method and question and answer section. Extension activities were carried out on Wednesday, October 26, 2022 at 09.00 to 12.30 WIB at the Margo Lestari Village Hall, Jati Agung District, South Lampung Regency. The socialization was attended by 20 people, most of whom were breeders. After carrying out community service activities, it can be concluded that there are several things obtained by farmers as participants, namely increasing knowledge related to processing poultry farm products into salted eggs to increase the selling price and shelf life of production eggs in an effort to increase the economic value of farmers. Keywords: Processed Livestock Products, Economic Value, Egg Storability

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