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Bioma
ISSN : 01263552     EISSN : 25809032     DOI : -
Core Subject : Agriculture,
Bioma is a national peer-reviewed and open access journal that publishes significant and important research from all area of biosciences fields such as biodiversity, biosystematics, ecology, physiology, behavior, genetics and biotechnology. All life forms, ranging from microbes, fungi, plants, animals, and human, including virus, are covered by Bioma.
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Articles 115 Documents
Uji Efektivitas Vaksin Flu Burung Subtipe H5N1 pada Ayam Kampung di Legok, Tangerang, Banten Arria Janovie; Rusdi Rusdi; Atin Supiyani
Bioma Vol 10 No 2 (2014): Bioma
Publisher : Biologi UNJ Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (131.292 KB) | DOI: 10.21009/Bioma10(2).6

Abstract

Abstract The aim of the study was to determine the effectiveness of Avian Flu Vaccine Subtype H5N1 on native chicken. There were several inclusion criteria to selected the chicks. The criteria were just native chicken in Legok, at least 8 weeks old and never been had previous vaccine program. Total of 20 chickens were used as samples. All chicken was split in two cages, cage A contained 10 chickens were treated with vaccine and cage B contained 10 chickens as a control. Then 0.5 mL of vaccine administered intramuscularly in the chicken breast. The effectiveness of vaccination determined by measuring antibody titers before and 3 weeks after vaccination program. Measurement of antibody titers obtained by HI (Hemagglutination Inhibition) test method. Result showed that antibody titers of native chicken increased after vaccination program. There were 70% of samples that have been vaccinated could be protected against the virus. Three chickens as a control had positive titers prior to vaccination with a range of 21 - 22. Key Word : test of effectiveness, homologue killed vaccine, antibody titers.
Keragaman Karakter Morfologi, Komponen Hasil, dan Hasil Plasma Nutfah Kedelai (Glycine max L.) Priskilla Purnaning Putri; Adisyahputra Adisyahputra; Asadi Asadi
Bioma Vol 10 No 2 (2014): Bioma
Publisher : Biologi UNJ Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (345.057 KB) | DOI: 10.21009/Bioma10(2).7

Abstract

Abstract Soybean (Glycine max L.) is annual crop that have high morphologies and yield components diversity. The research was conducted at the first season of 2011, the objective of the research were to find morphological, yield, and yield component of Soybean germplasm (Glicine max L.). The research was carried out at experimental station BB-BIOGEN Citayam, Depok, and laboratory of Gene Bank BB-BIOGEN. The experiment used randomized block design with 100 different accessions and three replications for each accession. Based on the observation, the morphological characters have many visual forms. They are as follows: growth percentage in which 19.33 – 99%; growth types were determinate and indeterminate, the leave form was triangle to sharp; purple and white flowers; yellow and black seeds color. The range of values for each characteristic component are as follows: plant height 29,23 – 104,25 cm; number of pods per plant was 23,6 – 99,82; flowering time 33 – 47 days after planting; 100 seed weight 5,98 – 20,77 gram; maturing time 75 – 96,67 days after planting; root nodule’s weight 0,004 – 0,109 gram; seed’s weight 3,15 – 11,45 gram/plant. Among the accessions, the highest yield was shown by B 4323 (643,27 gram/3,6 m2). Significant correlation was shown between soybean’s yield components and yield which were plant’s height, growth percentage, numbers of main stem’s node, numbers of pods, seeds weight for each plant and root nodule’s weight. 100 seeds weight showed significant negative correlation with soybean components. Key words: germplasm, morphological characteristics, soybean, yield components
KADAR PEROKSIDA LIPID DAN AKTIVITAS SUPEROKSIDA DISMUTASE SERUM DARAH PADA PENDERITA DIABETES MELITUS TIPE 2 HENI KRISTINA; NURMASARI SARTONO; RUSDI RUSDI
Bioma Vol 11 No 1 (2015): Bioma
Publisher : Biologi UNJ Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (457.585 KB) | DOI: 10.21009/Bioma11(1).1

Abstract

ABSTRACT Prevalence of type 2 diabetes mellitus rapidly increase in industry and developing country. Oxidative stress was estimated has role in development of type 2 diabetes mellitus. This study was aim to measure levels of malondialdehyde (MDA) and activity of superoxide dismutase (SOD) in type 2 diabetes mellitus, and also to analyse the relationship among both. This study was conducted on July-October 2014, using Ex Post Facto method and cross sectional design. The result showed that MDA serum levels significantly higher in diabetes mellitus patient compare to normal (P=0,000). Activity of superoxide dismutase (SOD) in diabetes mellitus serum has no significant difference with normal (P=0,290).There was possitive correlation between levels of MDA and activity of SOD in type 2 diabetes mellitus but not significant (P=0,478) with rs=0,199, thus also in normal subject (P=0,194) with rs=0,355. There was no significant different between two correlation coefficient (P=0,6781). In conclusion, there was significant different level of MDA, there was no significant different activity of SOD, there was positive correlation but not significant between levels of MDA and activity of SOD serum in type 2 diabetes mellitus and normal. And there was no significant different between two correlation coefficient. Keywords: malondialdehyde, superoxide dismutase, type 2 diabetes mellitus
PERTUMBUHAN DAN AKTIVITAS ANTIOKSIDAN DARI MIKROALGA MONIKA RAFAELINA; YOSWITA RUSTAM; SRI AMINI
Bioma Vol 11 No 1 (2015): Bioma
Publisher : Biologi UNJ Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (486.647 KB) | DOI: 10.21009/Bioma11(1).2

Abstract

ABSTRACT Microalgaebiomass containschemicalcomponentssuch as vitamin, proteins, ω-3 and ω-6 polyunsaturated fatty acid and pigments. components with antioxidant activities can be found in only a few species of algae. The potential species of microalgae who have compounds act as antioxidant are Porphyridium cruentum and Chlorella sp. This study aimed to know the growth and antioxidant activity of P. cruentum and Chlorella sp. with the treatment of different cultures. Experimental method and completely randomized design was used as the research method. Antioxidant activity (Ic50 value) was measured by DPPH method (2,2-diphenyl-1-pikrilhidrazil). SPSS 16.0 is used to analyze the data with General Linier Model (GLM) Univariate. The results of GLM Univariate test for cell density showed that there was significant different for cell density in species factors (p= 0,000), but there was no significant different for cell density in culture factors (p= 0,375), and GLM Univariate test for antioxidant activity showed that no significant different for antioxidant activity in species factors (p= 0,522) but siginificant different for antioxidant activity in culture factors (p= 0,001). There was significant different for growth in P. cruentum and Chlorella sp. but no significant different in indoor and outdoor culture, as well as there was no significant different antioxidant activity in two species but significant different in indoor and outdoor culture. Keywords: growth, antioxidant activity, indoor cultivation, outdoor cultivation.
HUBUNGAN KARAKTERISTIK LANSKAP DENGAN PREFERENSI SITUS OVIPOSISI KUPU-KUPU DI RUANG TERBUKA HIJAU KAWASAN JAKARTA TIMUR PUTRI DIANA; REFIRMAN DJAMAHAR; HANUM ISFAENI
Bioma Vol 11 No 1 (2015): Bioma
Publisher : Biologi UNJ Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1020.786 KB) | DOI: 10.21009/Bioma11(1).3

Abstract

ABSTRACT Urban area is dominated by land that functioned of the interest of economy and settlement, but only a few land allocated for wildlife. The butterflies was one of the wildlife that could be found in urban areas.The remaining habitat that can be used by butterflies assumed confined to the urban green space. Based on its life cycle, the butterflies having an initial phase (egg to larvae) is a phase which is generally require specific habitat. This research aims to determine the relationship between landscape characteristic and oviposition site preferences of butterfly. This research was conducted on April to June, 2014 at fifteen urban green spaces in East Jakarta by using descriptive survey technique. Landscape characteristics measured include area, perimeter, lawn area, closed vegetation area, open vegetation area, non vegetation area. Landscape characteristic not only measured from urban green space, but also measured from the area around urban green space within the scope of 100 meters buffer. Results show that there is a relationship between landscape characteristic and oviposition site preferences. Significant positive correlation between the abundance and area correlation coefficients rs (0,546), open vegetation area rs (0,758) and non vegetation buffer area rs (0,688). There was no significant correlation between the abundance with perimeter area, lawn area, closed vegetation area, non vegetation area, lawn buffer, open vegetation buffer and closed vegetation buffer. Keywords: caterpillar,landscape characteristic, oviposition site, preferences, urban green space
PENGARUH AKTIVITAS FISIK MAKSIMAL DAN EKSTRAK DAUN BAMBU MANGGONG (Gigantochloa manggong) TERHADAP AKTIVITAS KATALASE PADA HATI TIKUS PUTIH (Rattus norvegicus) RIRIEN APRIANA APRIANA; SUPRIYATIN SUPRIYATIN; SRI RAHAYU
Bioma Vol 11 No 1 (2015): Bioma
Publisher : Biologi UNJ Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (392.961 KB) | DOI: 10.21009/Bioma11(1).4

Abstract

ABSTRACT Gigantochloa manggong, one of endemic bamboo plant in Indonesia is suspected have potensial as an exogenous antioxidant. Exogenous antioxidant can help the activity of endogenous antioxidant on the body when overtraining occurs. Antioxidant can be measured by catalase enzymes activity. This present study was carried out to determine the effect of maximum physical activity and leaf bamboo extract on catalase activity in liver organ of rats. This research used experimental method with completely randomized design (cRD). The test groups were the control rats (E0R0), rats induced leaf extract (E1R0), rats treated swimming activity (E0R1) and rats induced leaf extract and swimming activity (E1R1). Data were analyzed by the two-way ANOVA statistical test. Bamboo leaf extract turned out to be non-toxic and leaf extract contained flavonoids, alkaloids, triterpenoids dan saponins. Catalase units in the control group = 1.00 unit/ml, the group which induced leaf extract = 0.89 unit/ ml, the group which treated maximum physical activity = 0.78 unit/ml and the group which treated maximum physical activity and induced leaf extract = 0.56 unit/ml. Based on statistical test, catalase activity no effect (p> 0,05) on rats. It was concluded that maximum physical activity can’t decreased catalase activity, leaf bamboo extract can’t increased catalase activity and there was no effect between maximum physical activity and manggong bamboo leaf extract on catalase activity in liver organ of rats. Keyword : antioxidant, catalase, manggong bamboo, physical activity
ISOLASI DAN KARAKTERISASI BAKTERI PENGHASIL SENYAWA ANTIBAKTERI YANG BERASOSIASI DENGAN KARANG BATU DARI PERAIRAN BITUNG DAN SPONS DARI SELAT MAKASSAR FRANSISCA SUNNY; TRI HANDAYANI KURNIATI; ARIANI HATMANTI
Bioma Vol 11 No 1 (2015): Bioma
Publisher : Biologi UNJ Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (369.545 KB) | DOI: 10.21009/Bioma11(1).5

Abstract

ABSTRACT Recently the needs of antibacterial compounds is increasing. This is due to the bacterial resistence to common antibacterial compounds. coral and sponge-ssociated bacteria are potential producer of antibacterial compounds. This research was aim to obtain coral and sponge-associated bacteria that could produce antibacterial compound. coral associated-bacteria was isolated from Bitung and was isolated in Marine Agar by pour plate method. The antibacterial compounds were obtained by extraction using ethyl acetate and acetone. The antibacterial assay was performed by agar diffusion method using paper discs and was performed by testing with Staphylococcus aureus, Bacillus subtilis, Vibrio cholerae biotipe El Tor, and Escherichia coli. Total 37 isolate was isolated from corals and 25 isolate from sponge obtained from Selat Makassar. Based on the assay, only bacteria from sponge that showed antibacterial activity. Two sponge-associated bacteria, S.5-8 and S.2-1 NRBC were found to inhibit S. aureus. From those isolates, isolate S.5-8 produced bigger clear zone (2,6 mm) than S.2-1 NRBC (1,5mm). S.5-8 could hydrolize gelatine whereas S.2-1 NRBC showed positive result on oxidase test and was able to fement xilose and arabinose to produce acid. Key words: antibacterial activity, association, characterization, coral, isolation, sponge
KADAR Na+, K+, Cl-, DAN KALSIUM TOTAL SERUM DARAH SERTA HUBUNGANNYA DENGAN TEKANAN DARAH PADA PENDERITA HIPERTENSI PUSPITA ANGGRAINI; RUSDI RUSDI; ERMITA IBRAHIM ILYAS
Bioma Vol 11 No 1 (2015): Bioma
Publisher : Biologi UNJ Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (372.227 KB) | DOI: 10.21009/Bioma11(1).6

Abstract

ABSTRACT Hypertension is currently still a major problem in the world. In Indonesia, the number of hypertension tends to increase every year. One of the causes of hypertension is high salt consumption. This research was held to identify levels of Na+, K+, Cl- and total calcium in blood serum and to analyze the relationship among levels of Na+, K+, Cl- and total calcium with blood pressure. This study was conducted on March to May 2014 using a Crosssectional method. The result showed that Na+ level in hypertensive patient significantly higher compared in normotensive (p=0.000). K+ level in hypertensive patient significantly lower than normotensive (p=0.002). In addition, no significant between Cl- level (p=0.514) and total calcium level (p=0.417) in hypertensive patients and normotensive subject. There was a significant relationship between Na+ level with systolic blood pressure (rs=0.419) and diastolic blood pressure (rs= 0.455). There was a significant relationship between K+ level with systolic blood pressure (rs=-0.403) and diastolic (rs=-0.496). There was no significant relationship between Cl- level with systolic blood pressure (rs=0.071) and diastolic (rs=0.092). There was significant relationship between total calcium level with systolic blood pressure (rs=-0.393) and no significant with diastolic (rs=-0.306). In conclusion, there were significant differences in the level of Na+ and K+ in hypertensive patient and normotensive and a significant relationship among level of Na+ and K+ with blood pressure. There were no significant differences in the levels of Cl- and total calcium in hypertensive patient and normotensive and no significant relationship among levels of Cl- and total calcium with blood pressure. Key Words: blood pressure, Cl-, hypertension, K+, Na+, total calcium
PERBANDINGAN PERILAKU HARIAN BURUNG BAYAN JANTAN (Eclectus roratus Müller, 1776) BERDASAKAN KEBERADAAN PEJANTAN PESAING DI TAMAN MARGASATWA RAGUNAN ANDES SACHRAN DESMUDZAT; PASKAL SUKANDAR; ADE SURYANDA
Bioma Vol 11 No 1 (2015): Bioma
Publisher : Biologi UNJ Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (401.997 KB) | DOI: 10.21009/Bioma11(1).7

Abstract

ABSTRACT Eclectus is one of many birds in Indonesia. The condition at captive may vary to the bird’s natural habitat. The objective of this research was to find out the comparison of daily behavior between two males Eclectus parrot at Ragunan Zoological park. Each males has different condition. The first male has a competitior in its cage, and the second male hasn’t. This research conducted January 2015 until March 2015. The behavior were ingestive, movement, motionless, and sexual. The descriptive method was used in this study. The focal animal sampling was used as a technique for recording those behavior. U Mann Whitney statistical test (p< 0,05) showed there are significant differences in the frequency of motionless and sexual behavior between male with competitor and male with no competitor. However, there is no significant diffrences in ingestive and movement behavior (p< 0,05). Keywords : Eclectus parrot, behavior, competitor, Ragunan
MULTIPLIKASI TANAMAN NILAM (Pogostemon cablin Benth) var. TAPAK TUAN SECARA IN VITRO SITI ASIYAH; CHRISTIANI TUMILISAR; TUTI LESTARI
Bioma Vol 11 No 1 (2015): Bioma
Publisher : Biologi UNJ Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (369.169 KB) | DOI: 10.21009/Bioma11(1).9

Abstract

ABSTRACTTissue culture is a technique widely used for propagation and improvement of crop quality. One way of plant propagation can be done with in vitro techniques. The purpose of the study was to determine the combinations and concentrations of NAA and BAP are appropriate for plant propagation patchouli. Dependent variable is the time of initiation of callus and shoots, callus texture, color callus, shoot number, and the number of roots patchouli. Independent variable is the concentration of growth regulators NAA and BAP. The method in this study is the experimental method. Data were analyzed by two-way Anova test followed by Duncan’s Multiple Range Test (DMRT). The results showed the stem explants concentrations of NAA and BAP best to induce callus is without NAA and BAP (control), 0.1 ppm NAA without BAP, NAA + BAP 0.5 ppm 0, while the leaf explants is there a 0.1 ppm NAA without BAP, NAA 0.1 ppm BAP + 0.5 ppm, 0.5 ppm BAP without NAA and NAA + BAP 0:01 ppm 1 ppm. Highest number of shoots obtained at a concentration of 0 ppm NAA concentration and NAA and 0.01 for leaf explants at a concentration of 0:01 ppm NAA + BAP 1 ppm and 0 ppm NAA + BAP 0.5 ppm for stem explants. Highest number of roots was found in 0:01 ppm for NAA concentration and leaf explants at a concentration of 0.1 ppm NAA + BAP and NAA 00:01 0 ppm ppm + 0.5 ppm BAP stem explants. Results of statistical analysis showed that there were significant differences of NAA and BAP concentrations on plant propagation patchouli (Pogostemon cablin Benth). Keywords: tissue culture, NAA, BAP, plant nilam (Pogostemon cablin Benth)

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