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INDONESIA
Biosaintifika: Journal of Biology & Biology Education
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 1,051 Documents
Selection of Cuculidae to the Hosts Based on the External Characteristics of the Eggs Widodo, Wahyu; Widiyaningrum, Priyantini
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.9939

Abstract

Most of Cuculidae were known as parasitic birds, and they breed depend on their hosts. The obyective of the study was to know the external characteristics of the eggs of Cuculidae and their hosts which includes several components. e.i. shell color, shape, length, diameter and egg index. Those species of Cuculidae were Cacomantis merulinus lanceolatus, Cacomantis variolus sepulcralis, Surniculus lugubris lugubris and Eudynamys scolopaceus malayanus. There were 117 item of the bird egg reference’s collections in the Ornithology Laboratory, Zoology Division, Research Center for Biology, LIPI, in Cibinong, used as a research materials. Color and form the egg from every sample noted by pursuant to direct eyesight with the eye if possible and assisted with the magnifier, especially at flimsy spots egg’s color. The results shown that egg size of Cuculidae groups are bigger than their host eggs. Generally, the color variation of eggs of Cuculidae and their hosts are white or bluish white and their combination like as brown and greenish. The eggs shape of Cuculidae and host are similar, namely oval with IFO value about 73 - 75%.  The Cuculidae family pays attention to the color, spot pattern and shape of the host’s eggs in choosing the host for their own eggs. Though, there was also a tendency not to recognize the characteristic of the host’s egg. The studies represent an early stage in an attempt to strive the conservation of Cuculidae the bird hosts.
Flavonoid Production in Callus Cultures from Mesocarp of Stelechocarpus burahol Habibah, Noor Aini; Moeljopawiro, Sukarti; Dewi, Kumala; Indrianto, Ari
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 2 (2016): September 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i2.6632

Abstract

Stelechocarpus burahol is one of the medicinal plants that contains flavonoids. The study was carried out to know flavonoid production of cultures in vitro S. burahol from mesocarp explants. Mesocarp explants were cultured on MS medium containing different combination and concentration of plant growth regulators i.e. picloram (5, 7.5 and 10 mg/L) and 2, 4-D (10, 15 and 20 mg/L) under dark condition. Induction of callus formation started on the 20.29th to the 29.86th days. Medium supplemented with Picloram and dark state proved to be the best condition for optimum callus induction from mesocarp explants of S. burahol. Callus grown on medium with the addition of 7.5 mg/l Picloram produces the highest flavonoid. The maximum production of the secondary metabolite was obtained from 8 weeks old callus. However, by the time of callus ageing, its output has declined. It could be concluded that callus cultures from mesocarp S. burahol can be used for flavonoid production.How to CiteHabibah, N. A., Moeljopawiro, S. Dewi, K. & Indrianto, A. (2016). Flavonoid Production in Callus Cultures from Mesocarp ofStelechocarpus burahol. Biosaintifika: Journal of Biology & Biology Education, 8(2), 214-221.
Struktur Mikroanatomi Hati dan Kadar Kolesterol Total Plasma Darah Tikus Putih Strain Wistar Pasca Suplementasi Minyak Lemuru dan Minyak Sawit Surasa, Ngudy Jaka; Utami, Nur Rahayu; Isnaeni, Wiwi
Biosaintifika: Journal of Biology & Biology Education Vol 6, No 2 (2014): September 2014
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v6i2.3778

Abstract

Mengkonsumsi minyak lemuru dan minyak sawit secara terus menerus, diduga dapat menyebabkan kerusakan mikroanatomi hati. Penelitian ini bertujuan untuk mengetahui struktur mikroanatomi hati dan kadar kolesterol total plasma darah tikus putih strain Wistar paska suplementasi minyak lemuru dan minyak sawit. Penelitian ini merupakan penelitian eksperimental, dengan rancangan acak lengkap. Penelitian menggunakan 25 ekor tikus usia 3 bulan. Masing-masing diberi minyak sawit dan minyak lemuru sebanyak 0%+0%, 5%+5%, 10%+10%, 15%+15%, dan 20%+20% secara per oral selama 28 hari. Data kadar kolesterol total plasma darah, berat badan tikus dan berat hati tikus dianalisis secara kuantitatif menggunakan ANAVA satu arah, dengan tingkat kesalahan 5%. Hasil penelitian menunjukkan bahwa pemberian minyak sawit dan minyak lemuru bersifat toksik terhadap hati dan dapat menurunan kadar kolesterol total plasma darah. Sifat toksik minyak lemuru dan minyak sawit terhadap hati dapat dilihat dari adanya perubahan struktur mikroanatomi hati, makroskopis hati tikus, dan berat hati tikus. Kerusakan struktur mikroanatomi hati yang ditemukan berupa kerusakan sel hati (nekrosis hati) serta melebarnya sinusoid. Kesimpulan penelitian ini adalah pemberian minyak sawit dan minyak lemuru mengakibatkan kerusakan mikroanatomi berupa nekrosis sel hati, sehingga mempengaruhi makroskopis hati (perubahan tekstur dan warna), berat hati, dan mempengaruhi berat badan tikus.Consuming lemuru oil and palm oil continuously could be expected to cause damage to the liver micro-anatomy. This study aimed to determine the micro-anatomy structure of liver and blood plasma total cholesterol level of white rats Wistar strain by post-oil supplementation of lemuru and palm oil. This research was experimental, with a completely randomized design. The research used 25 mice aged 3 months. Each was given a palm oil and lemuru oil as much as 0%+0%, 5%+5%, 10%+10%, 15%+15%, and 20%+20% orally for 28 days. Blood plasma levels of total cholesterol, body weight of rats and mice liver weight quantitatively analyzed using one-way ANOVA, with an error rate of 5%. The results showed that the palm oil and lemuru oil was toxic to the liver and decreased the blood plasma total cholesterol levels. Toxicological properties lemuru oil and palm oil to the liver can be seen from the change in the micro-anatomy structure of heart, liver macroscopic rat, and mouse liver weight. Micro-anatomy structural damage on liver cells were found in the form liver necrosis as well as the widening of the sinusoid. The conclusion of this study was the provision of palm oil and lemuru oil was caused damage to microscopic liver cell necrosis form, thus affecting the macroscopic liver (changes in texture and color), a heavy heart, and affect the body weight of rats.
Correlation of Physical-Chemical Parameters to Total Coliform Value in Jawi River, Pontianak, West Kalimantan Rahmawati, Rahmawati; Fitria, Laili; Syafitri, Liza
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.10187

Abstract

Coliform bacteria can be used as an indicator of the presence of pathogenic bacteria, such as E.coli bacteria that cause diarrhea. The aimed of this study is to determine the relationship between physical-chemical parameters namely temperature, pH, DO and BOD to the density of coliform bacteria in Jawi River, Pontianak. The sampling was conducted at one point each in the upstream, midstream and downstream area of the Jawi River during two tidal conditions of the Kapuas River in September 2016 at 09:40 (at low tide) and at 15:40 (at high tide). The correlation of physical-chemical parameter to coliform value was tested Pearson Product Moment. The results showed that coliform bacterial density increased from upstream to downstream with 150-1500 MPN/100 ml at high tide and 930-11000 MPN/100 ml at low tide. The results showed that the coliform bacterial density value had a positive relation with pH and BOD parameters and negative relation with temperature and DO parameters. So, it can be concluded that there is a correlation between physical parameters, such as temperature and chemical parameters such as pH, DO and BOD to microbiological parameters especially Coliform bacterial density. The benefit of this study is to give information about water quality of Jawi River and its correlation with density of Coliform bacterial, so that people are expected to pay more attention to the use of clean water to avoid the disease caused by coliform.
Effect of Electron-Beam Irradiation on the Growth Profile and Fatty Acid Composition of Botryococcus sp. Yuniarsih, Windra; Halimatul, Heli Siti; Wulandari, Asri Peni
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.14891

Abstract

Botryococcus sp. is an economically important microalgae as it contains carbohydrates, lipids, proteins, and a number of phytochemicals. The high concentrations of saturated fatty acids (SFAs) and monounsaturated fatty acids (MUFAs) in this strain also a valuable source for production of biofuel. Mutagenic engineering using irradiation has been known to increase the content of these biomass. The aim of this study is to know the effect of electron-beam irradiation to the growth profile and fatty acid composition of Botryococcus sp. In this study, Botryococcus sp. adjusted an optical density 1.0 after being exposed to different doses of electron-beam irradiation (160 kGy, 240 kGy, and 320 kGy) and induced random mutagenesis for strain improvement was observed based on high energy (1.5 MeV and 2 mA). Several mutants obtained were designated as strain B160, B240, and B320, respectively. The profile growth was significantly different between control (0 kGy) and irradiated microalgae strain. The highest growth is found in B320 mutant. Fatty acid of Botryococcus sp. control produced 7 fatty acids, B160 produced 7 fatty acids, B240 produced 6 fatty acids, whereas B320 produced 9 fatty acids. B320 produced hydrocarbon and phtalic acid as well as fatty acids. Total SFAs and MUFAs of B240 was increased 1.6 times compared to the wild type. The results would give some implications to improve the quality of biodiesel from Botryococcus sp.
Aplikasi Pupuk Kandang Kotoran Ayam pada Tanaman Kacang Tanah (Arachis Hypogeae L.) Marlina, Neni; Aminah, Raden Iin Siti; -, Rosmiah; Setel, Lusdi Ramlan
Biosaintifika: Journal of Biology & Biology Education Vol 7, No 2 (2015): September 2015
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v7i2.3957

Abstract

Pupuk kandang kotoran ayam diharapkan dapat memperbaiki sifat fisik, kimia dan biologi tanah, sehingga dapat menyuburkan tanah dan membantu dalam menyumbangkan unsur hara yang dapat digunakan dalam meningkatkan hasil kacang tanah. Penelitian ini bertujuan untuk mendapatkan takaran pupuk kandang kotoran ayam yang tepat dalam meningkatkan produksi tanaman kacang tanah. Penelitian ini telah dilaksanakan di kebun petani di Desa Payakabung Kecamatan Indralaya Utara Kabupaten Ogan Ilir dari bulan Januari sampai dengan April 2014. Rancangan yang digunakan pada penelitian ini adalah Rancangan Acak Kelompok (RAK) dengan tiga perlakuan dan delapan kelompok, sehingga berjumlah 24 petak penelitian dan setiap petak diambil 10 tanaman sebagai sampel . Perlakuannya adalah takaran pupuk kandang kotoran ayam 5, 10 dan 15 ton ha-1. Hasil penelitian menunjukkan bahwa takaran pupuk kandang kotoran ayam sebanyak 10 ton ha-1 memberikan pertumbuhan dan produksi terbaik dengan ditunjukkan produksi per petak sebesar 2,73 kg petak-1.Poultry manure is expected to improve soil physical, chemical and biological properties. It can improve soil fertility and help in nutrients contribution that can be used to increase the yield of peanut. This study aimed to get the right dose of poultry manure fertilizer in increasing the production of ground peanut plants. This research was conducted in farmyard in the North Indralaya Payakabung District of Ogan Ilir from January to April 2014. The design used in this study was a randomized block design with three treatments and eight groups, thus consisting 24 research plots and each plot was taken as a sample of 10 plants. The treatments of poultry manure fertilizer rate 5, 10 and 15 ton ha-1. The results showed that poultry manure fertilizer rate as much as 10 tons ha-1 gave the best growth and production of 2.73 kg per plot.
Varian Kualitatif Kacang Tanah Hasil Kultur in Vitro dan Hasil Seleksi in Vitro (Qualitative Variants of Peanut Plants Obtained from in-Vitro Culture and in-Vitro Selection) Rahayu, Enni Suwarsi; -, Sudarso
Biosaintifika: Journal of Biology & Biology Education Vol 1, No 1 (2009): March 2009
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v1i1.37

Abstract

issue cultures that have passed callus phase can induce somaclonal variation, of which the intensity was influenced by the addition of selective agents to culture media. Somaclonal variations of peanut plant obtained from in-vitro cultured and in-vitro selected somatic embryos using PEG was not yet understood. The objectives of this research were 1) to identify the qualitative variants of peanut plant var. Kelinci obtained from in-vitro cultured and in-vitro selected somatic embryos using PEG, 2) to estimate the factors that control the qualitative variants. The non-selected and the selected variant somatic embryos of peanut were germinated and cultured. From fertile R0 lines, sufficient number of R1 and R2 progenies were grown for evaluation. The results showed that phenotypic variations of qualitative characters can be observed among R0, R1 and R2 generations of somaclonal lines. Variant phenotypes of qualitative characters can be observed, these include wide branching, excessive branching, leaf variegation, leaflet number abnormality, leaf pointed tip, ‘rosette’ leaf, complete sterility and partial sterility. The data indicated that wide branching, excessive branching, leaflet number abnormality, partial sterility and complete sterility were genetically controlled, while variant phenotypes of ‘rosette‘ leaf, leaf variegation, and leaf pointed tip were epigenetically controlled. Key words: somaclonal variant, qualitative characters, in vitro selection, in vitro culture
Grouping of Mangifera indica L. Cultivars of Subang West Java by Leaves Morphology and Anatomy Characteristics Cahyanto, Tri; Sopian, Ahmad; Efendi, Muhammad; Kinasih, Ida
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i1.8780

Abstract

The wide distribution and high species diversity produce large variation in morphology, anatomy and genetics of local mangoes. Until now, most mango classification system is based on the generative character while the vegetative character is neglected. Inventory of mango cultivars in Subang rarely studied, therefore the purpose of this study is to development foundation of identification methods basedthe anatomy and morphology of leavesof mango cultivars of Subang. The data could futher develop as baseline information about mango cultivar diversity in Indonesia. During study, 21 mango cultivars found in Subang, West Java. Leave samples were collected from the field and soaked in 70% alcohol. Leaf anatomy preparats made by whole-mount method and paraffin. The shape and type, location, size, density and index of stomata were observed. The results showed high similarity of all sampels.There are three variations of vascular tissues on petioles, letter U shape (planoconvex),U lobed (highlyconvex) and round (circular) with the type of drus crystal form, small cubes, and prismatic. All cultivars have actinositic stomata and hypostomatatic leaves. It could be concluded that density, size and stomata index are less powerful to be apply as characters to identified cultivars of mangoes however the presence of oxalate crystals can be use to the grouping mango cultivars in Subang.
Ecological Factors Determining Abundance of Parasitic Mites on Aedes spp. Larvae Firmansyah, Nurhadi Eko; Soviana, Susi; Budianto, Bambang Heru
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.11458

Abstract

Ability to infestation and abundance of parasitic mites in Aedes spp. larvae cannot be separated from the influence of various factors. Ecological factors have been suggested to play a role determine the presence of parasitic mites that under certain conditions become a key factor in determining the abundance of parasitic mites on Aedes spp. larvae. The aim of this study to determine the ecological factors affect the abundance of parasitic mites on Aedes spp. larvae in Bogor Regency. Capturing of Aedes spp. larvae was performed directly on the habitats found in indoor and outdoor. Capturing mites in the body of Aedes spp. larvae was performed using insect forceps. Ecological factors measured were dissolved oxygen (DO), pH, temperature, and total dissolved solid (TDS). The influence of ecological factors was analyzed using regression and correlation analysis. The result of mite identification has been obtained three species of mites that are Halacarus sp., Histiostoma sp., and Hydrozetes sp. The result indicated that total dissolved solid (TDS) and temperature was the factors that determined the abundance of mites. The factors of pH, and dissolved oxygen (DO) did not determine the abundance of parasitic mites of Aedes spp. larvae. The research result can be further developed as a new alternative to Dengue Hemorraghic Fever control and provide information on parasitic mites that infest Aedes spp. larvae. In addition, this results become an early step in controlling of Aedes spp. strategy platform by the parasitic mites.
Harmful Algal in Banyuasin Coastal Waters, South Sumatera Aryawati, Riris; Bengen, Dietriech Geoffrey; Prartono, Tri; Zulkifli, Hilda
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 2 (2016): September 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i2.6356

Abstract

Phytoplankton have important as food-chain major component and primary production of marine environment. However, high abundance of phytoplankton could give harmful effects toward water ecosystem. Moreover, they could produce toxic substances that will be accumulated within their consumer. This accumulation could be dangerous for human or animals.This research were aimed to determine and calculatespecies of harmful algae in Banyuasin coastal waters. The study was conducted on April, June, August, October and December of 2013, and in February 2014, at ten stations. Phytoplankton samples were taken vertically using plankton nets. In the form of cone-shaped with a diameter of 30 cm, length 100 cm and mesh size 30 ?m.The result showed that there are 35 genera of phytoplankton. That have been found and consisted of four groups; Bacillariophyceae, Dinophyceae, Cyanophyceae and Chlorophyceae. 13 species were identified as Harmful Algal (Chaetoceros, Coscinodiscus, Nitzschia, Skeletonema, Thalassiosira, Alexandrium, Ceratium, Dinophysis, Noctiluca, Protoperidinium, Prorocentrum, Anabaena dan Oscillatoria), with seven of them were known for having toxin (Nitzschia, Alexandrium, Dinophysis, Protoperidinium Prorocentrum, Anabaena and Oscillatoria). Monitoring result showed that the highest number of species of potential harmful algal blooms (HABs) occured in June and the highest abundance occured in August, especially Chaetoceros and Skeletonema.How to CiteAryawati, R., Bengen, D. G., Prartono, T., & Zulkifli, H. (2016). Harmful Algal in Banyuasin Coastal Waters, South Sumatera. Biosaintifika: Journal of Biology & Biology Education, 8(2), 231-239.

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