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Dr. Ir. Dyah Rini Indriyanti
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biosaintifika@mail.unnes.ac.id
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Kota semarang,
Jawa tengah
INDONESIA
Biosaintifika: Journal of Biology & Biology Education
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 1,051 Documents
Identification and Cluster Analysis of Pitcher Plant (Nepenthes spp.) from South Sumatera Indonesia Lestari, Weni; Jumari, Jumari; Ferniah, Rejeki Siti
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.13968

Abstract

Nepenthes spp. is a typical plant of Southeast Asia especially Indonesia which has a special leaf modification called a pitcher. The largest number of Nepenthes spp. species in Indonesia is on the island of Sumatra. The purpose of this reseach was to identify and analyze cluster Nepenthes spp. from South Sumatra based on morphological characteristics. The specimens were collected from the forest of Tekorejo Village, Air Itam Village and cultivation location in Palembang city of South Sumatra. Identification of morphological characters performed on the characteristics of root, stem, leaves, and pitcher. The morphological data is used for cluster analysis using NTSYS software version 2.02. The identification results showed 9 variants of Nepenthes spp. which belong to the species N. mirabilis, N. gracilis, and N. sumatrana. Dendogram analysis results form two main clusters with a similarity value of 22%. The first cluster consists of N. mirabilis and N. sumatrana. The second cluster consists of N. gracilis. Based on the results of this study can be concluded that the species Nepenthes spp. South Sumatra is N. mirabilis, N. gracilis, and N. sumatrana. The results of this study will be dedicated to updating information about the existence of Nepenthes spp. from South Sumatra and his cluster.
Uji Kandungan Timbal (Pb) dalam Daun Tanaman Peneduh di Jalan Protokol Kota Semarang Istiaroh, Pawit Dwi; Martuti, Nana Kariada Tri; Bodijanto, F. Putut Martin Herry
Biosaintifika: Journal of Biology & Biology Education Vol 6, No 1 (2014): March 2014
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v6i1.3786

Abstract

Gas buang yang dihasilkan oleh kendaraan bermotor pengguna bahan bakar bensin bertimbal mengemisikan Pb ke dalam lingkungan dan berpotensi terserap ke dalam jaringan daun tanaman peneduh jalan. Penelitian bertujuan untuk menguji kandungan timbal (Pb) dalam daun tanaman peneduh di jalan protokol Kota Semarang. Sampel penelitian adalah daun angsana (Pterocarpus indicus Willd), glodokan (Polyalthia longifolia Bent & Hook. F) dan mahoni (Swietenia mahagoni (L.) Jacq.) yang dominan digunakan sebagai peneduh di lima jalan protokol Kota Semarang yaitu Jl. Kalibanteng, Jl. Pemuda, Jl. Brigjen Katamso, Jl. Kaligawe dan Jl. Setyabudi. Kandungan Pb dalam daun tanaman angsana, glodokan dan mahoni diuji menggunakan metode AAS (Atomic Absorption Spectrophotometery) di Laboratorium Balai Besar Teknologi Pencegahan Pencemaran Industri (BBTPPI) Semarang. Hasil penelitian ditemukan kandungan Pb dalam daun tanaman peneduh di jalan protokol Kota Semarang. Kandungan Pb dalam daun tertinggi 0,05 ppm/g daun basah terdapat pada tanaman angsana dan terendah sebesar 0,01 ppm/g terdapat pada tanaman glodokan dan mahoni. Dari hasil penelitian disimpulkan bahwa kandungan Pb dalam daun tanaman peneduh di jalan protokol Kota Semarang berkisar 0,01-0,05 ppm/g daun basah dan tergolong rendah dibawah kadar normal Pb dalam tanaman yaitu 0,5-3,0 ppm.Exhaust gases produced by motor vehicle users leaded gasoline emits Pb into the environment and potentially absorbed into the leaf tissue of plants along the roadside. The study aimed to examine the content of lead (Pb) in the shade plant leaves in the Semarang City road protocol. Samples were angsana leaves (Pterocarpus indicus Willd), glodokan (Polyalthia longifolia Bent & Hook. F) and mahogany (Swietenia mahagoni (L.) Jacq.). They were predominantly used as a shade in five main streets of Semarang is Kalibanteng St, Youth St, Brig Katamso St, Kaligawe St and Setyabudi St. The content of Pb in the Angsana, glodokan and mahogany leaves were tested using AAS (Atomic Absorption Spectrophotometery) method in Great Hall of The Pollution Prevention Technology Laboratory Semarang. The results showed that the content of Pb was found in the shade plant leaves in the Semarang road protocol. The highest Pb content in leaves was 0.05 ppm/g wet leaves at Angsana plants and the lowest was 0.01 ppm/g contained in mahogany and glodokan plants. Based on the results of this study, it concluded that the content of Pb in the shade plant leaves in the Semarang protocol ranged from 0.01 to 0.05 ppm/g wet leaves and relatively low under normal levels of Pb in plants is 0.5 to 3.0 ppm.
ISOLASI DAN IDENTIFIKASI KHAMIR SECARA MORFOLOGI DI TANAH KEBUN WISATA PENDIDIKAN UNIVERSITAS NEGERI SEMARANG -, Jumiyati; Bintari, Siti Harnina; Mubarok, Ibnul
Biosaintifika: Journal of Biology & Biology Education Vol 4, No 1 (2012): March 2012
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v4i1.2265

Abstract

Tujuan penelitian untuk mengisolasi dan mengidentifikasi khamir secara morfologi di tanah Kebun Wisata Pendidikan Unnes. Penelitian ini merupakan penelitian eksplorasi yang dilakukan di Laboratorium Mikrobiologi Jurusan Biologi FMIPA Unnes. Populasi penelitian adalah khamir di tanah Kebun Wisata Pendidikan Unnes. Sampel penelitian adalah isolat khamir yang diambil dengan teknik Purposive Sampling yaitu membagi kebun menjadi lima zona dan setiap zona diambil secara acak lima titik pengambilan kemudian dihomogenkan. Sampel dibawa ke Laboratorium untuk dilakukan isolasi, purifikasi dan identifikasi secara morfologi koloni dan sel. Simpulan penelitian ditemukan tujuh isolat khamir dan termasuk ke dalam lima genus yaitu Saccharomyces, Candida, Debaromyces, Brettanomyces dan Saccharomycodes. The purpose of the research was to isolate and to identify morphologically the individually-isolated yeasts from the soil of Biology Educational Garden Semarang State University. The exploration research was conducted in the Microbiology Laboratory Department of Biology, FMIPA Semarang State University. The yeasts were collected using purposive sampling technique in five zones and from each zone five random plots were selected to obtain the samples. Samples were isolated, purified and identified morphologically in terms of the colonies and the cells. Result revealed that seven isolated yeasts from five generas (Saccharomyces, Candida, Debaromyces, Brettanomyces, and Saccharomycodes) had been collected from the soil of Biology Educational Garden Semarang State University.
Cryopreservation of Aceh Swamp Buffalo (Bubalus bubalis) Semen with Combination of Glycerol and Lactose Eriani, Kartini; Sari, Nisa; Rosnizar, Rosnizar; Dasrul, Dasrul; Suhartono, Suhartono; Rizal, Muhammad
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (380.96 KB) | DOI: 10.15294/biosaintifika.v9i3.11426

Abstract

Aceh swamp buffaloes(Bubalus bubalis) are decreasing their population and genetic quality. This study was conducted to determine the influence of lactose and glycerol cryoprotectants on spermatozoa of Aceh swamp buffaloesafterthawing.This study used completelya factorial randomized design with nine treatments, and five replications.Fresh semen of the Aceh swamp buffalowere diluted by using a combination extender lactose cryoprotectants 0 mM (L0), 60 mM (L60), 120 mM(L120) and glycerol 3% (G3), 5% (G5), 7% (G7) with the equilibration of 4 hours.The results showed that the combination of cryoprotectants L120G7 influenced significantly (P < 0.05) on the quality of spermatozoa of the Aceh swamp buffalo(B. bubalis)after thawing.The percentage of sperm motility L120G7 (42.60 1.14);viability L120G7 (55.00 0.71);acrosome integrity L120G7 (52.00 0.71); and plasma membrane integrity L120G7(53.20 1.48). The combination of lactose cryoprotectants 120 mM (L120) and glycerol 7% (G7) was the best combination to maintain the quality of spermatozoa of swamp buffalo. This finding could be used to define a policy for the spermatozoa storage of Aceh swamp buffalo to artificial insemination (AI).
Neurogenic Differentiation of Bone Marrow Mesenchymal-Like Stem Cell Induced by Delonix regia Flowers Extract Ichsan, Ichsan; Boediono, Arief; Eriani, Kartini; Suryani, Irma; Azhar, Al; Nursanti, Risa
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.15051

Abstract

Stem cell technology has great potential in the effort to cure degenerative diseases. This study was done to determine optimum dose of flamboyant (Delonix regia) flower extract to induce proliferation and differentiation of mice (Mus musculus) bone marrow mesenchymal-like stem cell. Bone marrow cells were collected from mice by aspiration. Cells suspension (1 x 106) were poured into petri dishes containing 2 ml of modified Dulbeccos Modified Eagles Media (mDMEM) and incubated overnight at 37 °C in a 5% CO2 incubator and microscopically observed. In quadriplicate, MSC were cultured in mDMEM containing D. regia flower extract of 0.0 (control), 0.4, 0.6, 0.8, and 1.0 mg/ml and incubated at 37 °C for 9 days. Population doubling time (PDT) and differentiated cell type were microscopically observed using HE staining on day 1 and 10. Data obtained were analyzed by ANOVA and Tukey test. The results showed that the addition of D regia flowers extracts 0.8 and 1.0 mg/ml significantly reduced PDT compared to that of 0.4, 0.6 and control. The extract, at 0.4 and 0.6 mg/ml, were able to induce MSC differentiation into fibroblast-like and nerve-like cells. In conclusion, D. regia flower extracts of 0.6, 0.8 and 1.0 mg/ml were able to stimulate MSC proliferation, but optimum dose for neurogenic differentiation was 0.6 mg/ml. This is the first to show potential of D. regia flower extract as neurogenic differentiatian inducer on mice MSC. These findings can be used as preliminary information for using the extract as cellular differentian inducer in basic and applicative reseach using stem cells.
Ethnobotanical Study of Rattans on Suku Anak Dalam Community in Bukit Duabelas Nasional Park Mairida, Dwi; Muhadiono, Muhadiono; Hilwan, Iwan
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 1 (2016): March 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i1.5164

Abstract

Suku Anak Dalam (SAD), a tribal in Bukit Duabelas Nasional Park (TNBD) has knowledge for utilizing rattans. This research attempts to investigate the species of rattans utilized and to analyze the local wisdom of Suku Anak Dalam(SAD) community.The data was collected by doing an interview the informants consisting of temenggung (head of the tribe), jenang (the liaison between the villagers and anak dalam), rattans gatherers, induk (mother) and hand maker. Then, the researcher observed active participation (following the SAD activity in utilizing rattans).The interview was conducted based on the appropriate time with qualitative condition and situation. The result showed 12 (twelve) species of rattan utilized by the SAD community, Calamus ornatus (Blume), Calamus caesius( Blume), Calamus flabellatus (Becc.), Calamus manan(Miq.), Calamus csipionum (Lour.), Calamus javensis(Blume), Calamus axilliaris(Becc.), Calamus sp., Daemonorops geniculata (Griff.) Mart., Daemonorops draco(Willd.) Blume, Daemonorops verticiliaris(Griff.) Mart., and Korthalsiaechinometra(Becc.)The eight species were utilized as ambung, penampilan, and tekalo handicraft. One type for rituals, ropes, clotheslines, dye, durian lempok cakes preservatives (Durio spp.),and the species for the food for pregnant mothers (ngidam), and the three species for medicine. The implication of this research gave a documentation for the government to formulate and to make an authority of TNB forest conservation and to protect the culture of SAD community in the form of rattans inventarization and local wisdom of SAD community in utilizing the species of rattans.How to CiteMairida, D., Muhadiono, M., & Hilwan, I. (2016). Etnobotanical Study of Rattans on Suku Anak Dalam Community in Bukit Duabelas Nasional Park. Biosaintifika: Journal of Biology & Biology Education, 8(1) 64-70.
Pengembangan Edible Coating Ekstrak Daun Randu dan Pengaruhnya terhadap Kualitas Mentimun Widyastuti, Nawangwulan; -, Aminudin
Biosaintifika: Journal of Biology & Biology Education Vol 5, No 2 (2013): September 2013
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v5i2.2750

Abstract

AbstrakTujuan penelitian ini adalah untuk mengembangkan bahan edible coating (EC) alami dari ekstrak daun randu; serta mengetahui pengaruhnya terhadap mutu dan umur simpan mentimun. Rancangan penelitian menggunakan RAL dua faktor yaitu konsentrasi EC, (0%, 50%, 100%), dan suhu penyimpanan (suhu ruang dan suhu rendah). Parameter yang diukur adalah perubahan berat, total padatan terlarut (TPT), pH, warna, kekerasan dan kebusukan mentimun. Hasil penelitian menunjukkan bahwa penurunan berat mentimun yang disimpan pada suhu ruang paling rendah terjadi pada perlakuan kontrol (tanpa EC), sedangkan penurunan berat paling rendah pada penyimpanan suhu rendah adalah perlakuan EC konsentrasi 100%. Perlakuan terbaik untuk mempertahankan mutu (pH, TPT, warna, kekerasan dan kebusukan) terdapat pada kombinasi perlakuan EC ekstrak daun randu konsentrasi 100% dalam penyimpanan suhu rendah. Kombinasi perlakuan tersebut menghasilkan umur simpan mentimun yang paling lama sampai 9 hari.AbstractThe purpose of this study was (1) to develop edible coating material (EC) is a natural, easy to make and widely available, the ceiba pentandra leaf extract, and (2) to determine the effect of the EC material on the quality and shelf life of cucumber. Coating application with cucumber dipping way into the EC material. The design of the experiment made in RAL factorial, with material EC factors, concentrations of EC (0%, 50%, 100%), and storage temperature (room temperature and low temperature). Parameters measured were weight change, soluble solid content (SSC), pH, color, hardness and decay (senescence) of cucumber. The results of the study for the ceiba pentandra leaf extract showed the lowest weight loss of cucumber is in control (no EC or control) were stored at room temperature (about 8%), while at low temperatures is treated EC 100% concentration (about 8.7%). The best treatment to maintain quality (pH, SSC, color, hardness and decay) contained in the EC treatment combination of ceiba pentandra leaf extract 100% concentration with low temperature storage. The combination treatment its resulted in a shelf life of most long cucumber to 9 days.
The Dynamic of Phytoplankton Community Structure in Face of Warming Climate in A Tropical Man-Made Lake Sunardi, Sunardi; Febriani, Rina; Irawan, Budi; Saputri, Mutia Septi
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i1.7725

Abstract

In freshwater ecosystems, water temperature plays as an environmental factor that regulates its structure and function. A research on the impacts of changes in temperature to the dynamics of the Phytoplankton community structure has been done. Data from nineteen-year period (1995 to 2013) were collected from Cirata Reservoir as an example of artificial tropical lake in Indonesia. The research aimed to determine the changes of water temperature as the impact of climate warming on the dynamics of phytoplankton community structure. Different measures such as species richness, diversity index, and abundance were measured in order to understand the changes of phytoplankton community structure. Trend analysis, linear regression, and correlation were applied to achieve our objective. The study revealed that changes in water temperature have affected the species richness, but not the diversity index and abundance of the phytoplankton. Bacilariophyceae and Cyanophyceae were found as two predominant phytoplankton classes in the lake with percentage of 48,45 and 41,43 respectively, assuming their capacity to adapt the new environment. This study suggests that climate warming implies changes of the freshwater ecosystems.
Biodiversity of Termites and Damage Building in Semarang, Indonesia Subekti, Niken; Priyono, Bambang; Aisyah, Afrin Nur
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.12832

Abstract

Termites cause a lot of damage and big losses for building components, especially those made of wood. High population growth and rapid infrastructure development in Semarang city affect the natural habitats of termites. This phenomenon changes the termites behavior of foraging in buildings. Semarang city has humidity, temperature, and altitude that allows termites to live and breed well. The purpose of this study was to determine the types of termites living in housing in Semarang city as well as the level of attacks on housing. This research was conducted in nine housing in Semarang City. The research was carried out with two methods: building inspection and wood-feeding method by using Pinus mercusii wood measures 2 cm x 2 cm x 46 cm. The results found four types of termites that attack wood feeds namely Macrotermes gilvus, Microtermes inspiratus, Odontotermes javanicus, Coptotermes curvignathus and Cryptotermes sp.. Based on interviews and building inspection, it was known that the condition of the building in the category of "moderate" was occupying the most position (74.44%), good (17.78%) and lightly damaged (7.78%). From the causes of damage to building components, it can be concluded that the level of termite attack against damage to recidential buildings in Semarang city 44.58%. Cross-tabulation analysis of Chi-Square shows that there is a correlation between age and treatment with damage to the building. Futher, the finding can be used as baseline information for further scientific investigation for effective termites control on residential building components.
Efektivitas Seng (Zn) sebagai Imunostimulan dalam Produksi Reactive Oxygen Intermediate pada Mencit Balb/C yang Diinfeksi Salmonella typhimurium Yuniastuti, Ari; H, Nugrahaningsih W; Zunikhah, Zunikhah
Biosaintifika: Journal of Biology & Biology Education Vol 2, No 1 (2010): March 2010
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v2i1.1151

Abstract

This study aims to prove the effectiveness of zinc as a micronutrient to increaseimmunostimulatory Reactive Oxygen Intermediate (ROI) production in balb/c miceinfected by Salmonella typhimurium. Research was designed using randomized thePost Test Only Control Group Design. The sample of 24 male mice balb/c are groupedinto four groups, i.e. one control group and three treatment groups (treatment dose of 78ppm/day; 169 ppm/day and 260 ppm/day) intraperitoneally for 14 days with each groupconsisting of six rats. The independent variable is the provision of zinc with various doses,dependent variable is the ROI production of macrophages, which are checked by usingNitroblue Tetrazolium (NBT) Reduction Assay, and control variables are body weight,gender and age. All mice infected with Salmonella typhimurium 105 intraperitoneally onday 8th. At the end of treatment mice were killed and was examined for levels of ROI.Inspection results were analyzed by Kruskal Wallis test. Result showed that doses ofzinc of 78 ppm/day have immunostimulatory effective as the highest ROI productionof mice Balb/c infected with Salmonella typhimurium.Keywords: immunostimulant, Reactive Oxygen Intermediate, zinc (Zn)

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