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Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 15 Documents
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Search results for , issue " Vol 8, No 2 (2016): September 2016" : 15 Documents clear
The Effect of Organic Nutrient and Growth Regulators on Seed Germination, Embryo and Shoots Development of Dendrobium antennatum Lindl. Orchid by In Vitro Utami, Edy Setiti Wida; Hariyanto, Sucipto
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 2 (2016): September 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i2.5165

Abstract

Dendrobium antennatum has high economic value as cut flowers and flowerpots. Like orchid seeds in general, D. antennatum is difficult to germinate under natural conditions. This study aimed to determine the effect of coconut water on seed germination and embryo development, as well as the effect of NAA on shoots development of D. antennatum. This study consisted of two stages. In the first stage, the 12 weeks-old seeds after pollination were sown on MS medium containing 2 g/L peptone + 0%; 5%; 10%; and 20% coconut water. After 8 weeks of culture, the seeds germinated and the shoot formed were recorded. The highest in seed germination (92.2%) and the formation of shoots (51.4%) were obtained when seeds were cultured on MS medium containing 2 g/L peptone + 20% coconut water. In the second stage, the shoots were sub-cultured on MS medium containing 1 mg/L thidiazuron + 0 mg/L; 1 mg/L; 2 mg/L; and 3 mg/L NAA. After 16 weeks of sub-culture, the height of plantlets, the length of the roots and leaves, number of leaves and roots formed were recorded. MS medium containing 1 mg/L thidiazuron + 1 mg/L NAA was the most suitable for the shoots development of D.antennatum. The embryo development of D.antennatum in vitro begins with the enlargement of embryo, with further it emerges from the seed coat (germinated) followed by the formation of the apical meristems to form the shoots and the roots.How to CiteUtami, E. S. W., & Hariyanto, S. (2016). The Effect of Organic Nutrient and Growth Regulators on Seed Germination, Embryo and Shoots Development of Dendrobium antennatum Lindl. Orchid by In Vitro. Biosaintifika: Journal of Biology & Biology Education, 8(2), 165-171.
The Correlation Between Dietary Habits and Dental Hygiene Practice with Dental Caries Among School Children at Urban Area in Semarang Ali, Omaran Ibrahim Mohammed; Oedijani, Oedijani; Muis, Fatimah
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 2 (2016): September 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i2.6489

Abstract

Dental caries is a major cause of tooth loss in children and young adults. Dental caries have been linked to the situation of underprivileged families, nutritional imbalance, and poor oral hygiene techniques, including lack of tooth brushing or flossing the teeth, and also have a genetic etiology. Dietary habits and dental hygiene practice can result in high caries in school children. This research aimed to reveal the correlation between dietary habits and dental hygiene practice with dental caries among school children in urban area of Semarang. The subjects of this research are the elementary student 7 9 years old enrolled in schools located in at urban area in Semarang in 2016 and the mother of a student who became the study sample. Data were statisically analyzed usingbivariate analysis and multivariate analysis. Based on the research result, it can be concluded that: there was no correlation between total carbohydrate intake, refined carbohydrate intake, fiber intake, dental hygiene practice with dental caries, bottle feeding and duration of bottle feeding were assosiated with dental cariest-score. Overall, def-t score in the study was very bad with high median of dental caries score and many children have dental caries t-score more than 6.How to CiteAli, O. I. M., Muis, F. & O, Oedijani. (2016). The Correlation Between Dietary Habits and Dental Hygiene Practice with Dental Caries Among School Children at Urban Area in Semarang. Biosaintifika: Journal of Biology & Biology Education, 8(2), 178-184.
Growth Pattern and Copper Accumulation in Callus of Datura metel Nurchayati, Yulita; Santosa, Santosa; Nugroho, Laurentius Hartanto; Indrianto, Ari
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 2 (2016): September 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i2.5177

Abstract

This experiment was aimed to evaluate the copper accumulation using callus culture of Datura metel L. The culture was established from leaves onto MS contained NAA 2.5 mg/L and Kinetin 0.5 mg/L as the control. The exposure of the culture was carried out by 2 copper compounds as treatment, i.e. CuCl2.2H2O and Na2CuEDTA at level concentration 0.; 0.1; 5; 10; 15; and 20 M. The growth pattern of callus in control showed increasing growth rate in 36 days, whereas exponential stage was reached at 12-20th doi*. Whilst, after 10 doi, the treatment showed constant growth pattern. The absorption rate of the culture was increased by the addition of the CuCl2.2H2O at 5 15 M of level concentration but declined at 20M. The maximum rate of accumulation of Cu (0,1519 mg g-1) was obtained at 15 M. Instead, the addition of Na2CuEDTA at 5 20M of level concentration showed the significant increment while the maximum accumulation was obtained at 20M (0,1420 mg g-1). The existence of chelator in copper compound reduced the rate of toxicity while all tolerance index values were between 66,24 and 97,28 %.The results suggested the role of callus of D. metel as that fairly absorbed and accumulated Cu2+. Exposure with CuCl2.2H2O indicated higher accumulation than Na2CuEDTA.How to CiteNurchayati, Y., Santosa, S., Nugroho, L. H., & Indrianto, A. (2016). Growth Pattern and Copper Accumulation in Callus of Datura metel. Biosaintifika: Journal of Biology & Biology Education, 8(2), 135-140.
The Effectiveness of Experimental Diet with Varying Levels of Papain on The Growth Performance, Survival Rate and Feed Utilization of Keureling Fish (Tor tambra) Muchlisin, Zainal Abidin; Afrido, Fardin; Murda, Tanzil; Fadli, Nur; Muhammadar, Abdullah A.; Jalil, Zulkarnain; Yulvizar, Cut
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 2 (2016): September 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i2.5777

Abstract

The objective of present study was to determine the optimum level of papain in the diet of keureling fish (Tor tambra). The complete random design was utilized in this study. Six levels of papain dosage were tested in triplicates, i.e. 0 (control); 17.5 mg kg-1, 20.0 mg kg-1, 22.5 mg kg-1, 25.0 mg kg-1 and 27.5 mg kg-1 of feed. The experimental fish were fed the experimental diet two times a day at 8 AM and 5 PM at feeding level of 5% body weight for 90 days. The Anova test result showed that papain enzyme gave a significant effect on the weight gain, daily growth rate, specific growth rate, survival rate, feed conversion ratio and feed efficiency (P<0.05). The Duncan multi-rage test result showed that the higher values for all measured parameters were obtained at the dosage of 27.5 mg kg-1. Therefore, it is concluded that the optimum dosage of papain enzyme for keureling fish was 27.5 mg kg-1 of feed.How to CiteMuchlisin, Z. A., Afrido, F., Murda, T., Fadli, N., Muhammadar, A. A., Jalil, Z., & Yulvizar, C. (2016). The Effectiveness of Experimental Diet with Varying Levels of Papain on The Growth Performance, Survival Rate and Feed Utilization of Keureling Fish (Tor tambra). Biosaintifika: Journal of Biology & Biology Education, 8(2), 172-177.
Characterization of Three Species of Thrips on Weeping Fig, Nutmeg, and Marine Seruni Plants Based on Mtcoi DNA Sequences Kurniawaty, Nia; Hidayat, Purnama; Rauf, Aunu
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 2 (2016): September 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i2.5448

Abstract

Thrips are widely reported as pests in vegetable crops. However, the existence of Phlaeothripidae members has a less concern in Indonesia. Phlaeothripidae is the only family of Tubulifera Suborder and some reports suggested that they had potential to be pests in several crops due to their ability to roll up and to make galls on leaves. The first step in pest management attempt is to identify the pest accurately and quickly, so the pest management can be on target and more efficient. One of the identification methods is the molecular identification using DNA barcoding techniques. This study aimed to characterize and to compare species thrips in banyan, nutmeg, and marine seruni based on their molecular characteristics. This research was conducted in Bogor and Kuningan. The process of molecular characterization consisteds of four steps DNA total extraction, amplification by using PCR, COI gene sequence, and data analysis. PCR programme was succesfully to amplified mtCOI gene fragment at 710 bp. The length of mtCOI gene of Gynaikothrips uzeli, Haplothrips ganglbaueri, and Pseudophilothrips ichini were 704, 686, and 702 bp dominated by A and T bases with nucleotide variation value of 27.8%. This results confirmed that molecular characterization using mtCOI gene mitochondrial had successfully supported the morphological data.How to CiteKurniawaty, N., Hidayat, P. & Rauf, A. (2016). Characterization of Three Species of Thrips on Banyan, Nutmeg, and Marine Seruni Plants Based on Coi Gene. Biosaintifika: Journal of Biology & Biology Education, 8(2), 185-192.

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