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Biosaintifika: Journal of Biology & Biology Education
Published by Universitas Negeri Semarang
ISSN : 2085191X     EISSN : 23387610     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Biosaintifika Journal of Biology & Biology Education, an electronic international journal, provides a forum for publishing the original research articles, review articles from contributors, and the novel technology news related to biological research and biological education.
Arjuna Subject : -
Articles 1,051 Documents
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Screening and Isolation of Cellulolytic Bacteria from Gut of Black Soldier Flays Larvae (Hermetia illucens) Feeding with Rice Straw Supriyatna, Ateng; Ukit, Ukit
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6762

Abstract

A research on screening and isolation of cellulolytic bacteria from the gut of larvae Black Soldier treated rice straw feed has been conducted. The purpose of this study is to get the type of cellulolytic bacteria from the gut of larvae and bacteria that have the highest potential to degrade cellulose. Screening and isolation method applied by using intestinal larvae obtained from larval gut vortex at a speed of 1500 rpm. Furthermore, dilution graded from 1 to 10 and grown in media CMC (carboxyl methyl cellulose) at 37 0C and incubated for 48 hours. Observations were made based on the characteristics of the microscopic, macroscopic, biochemical test, cellulolytic activity and the activity of cellulase enzymes selected bacteria. The results showed a 9 cellulolytic bacteria from the gut of the larvae. Bacillus sp. is a bacteria that have the highest potential with cellulolitic activity 2.1 mm (dz/dk), the exponential phase of hour at the 24th, and cellulase enzyme activity of 0.4 U/mL at pH 7 and 0.41 U/mL at pH 8.This research showed that the Black Soldier Flays Larvae (Hermetia illucens) have competencein organic waste degradation, because in Black Soldier Flays Larvaes gut, cellulolitic enzyme is produced by cellulolitic bacteria, specially Bacillus sp,How to CiteSupriyatna, A., & Ukit, U. (2016). Screening and Isolation of Cellulolytic Bacteria from Gut of Black Soldier Flays Larvae (Hermetia illucens) Feeding with Rice Straw. Biosaintifika: Journal of Biology & Biology Education, 8(3), 314-320.
Genetic Variability of Local Corn Cultivars from Kisar Island Southwest Maluku Regency using Microsatellite Molecular Marker Sinay, Hermalina; Karuwal, Ritha Lusian
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.8512

Abstract

This research aim was to reveal the genetic variability of local corn cultivars from Kisar Island using microsatellite molecular marker. The use of microsatellite markers is based on one reasons that the genetic information is polymorphic, and will be better to identify genetic diversity of plants including corn. Corn cultivars used were obtained from farmer in Kisar Island, and three reference varieties as outgroup were obtained from Institute of Cereals in Maros South Sulawesi. Polymerase chain reaction was conducted following the protocols from Fast Start kit (Qiagen-USA). The data obtained then analyzed with MVSP 3.1A Software for dendogram construction. The results showed that the appearance of DNA bands was vary (monomorphic and polymorphic) for local cultivars as well as reference varieties, with one or two bands on each primer. Based on the dendogram, there are three main clusters with similarity index ranged from 20% -100%. It can be concluded that there is very low similarity and distant kinship of local cultivars. The novelty of this research is knowing kinship relationship between local corn in Kisar Island which is not known yet. This results is expected to provide benefits regarding the breeding program, and for instance, it can be an important information regarding the development of local corn as a source in assembly the superior corn cultivars.
Bryophytes in Cibodas Botanical Garden: Diversity and Potential Uses Nadhifah, Ainun; Khujjah, Madinatul; Vitara, Pantalea Edelweiss; Noviady, Ikhsan
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 2 (2018): August 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i2.14433

Abstract

Research on the diversity of bryophytes in Indonesia has been generally accomplished. However, the publication of potential uses of this tiny plant is very limited. Cibodas Botanical Garden (CBG) as a center for ex-situ plant conservation has potential climate conditions as a location of moss growth. The study aims to collect bryophytes in CBG and to identify their potential uses. Purposive sampling method was used on various substrate (soil, rocks, trees). Some environmental parameters were also recorded, for instance, substrate type, temperature, and humidity. All of bryophytes collections were taken to the laboratory for the identification and saved in herbarium (CHTJ). A literature study was conducted to determine the potential uses of identified moss species. From 153 collection numbers recorded in CBG, there were 42 species which have potential use. In addition, there were 42 species that have the potential to be used as a medicinal plant, ornamental plants, uses in agriculture and environmental services. This study provides the initial information about the potential uses of mosses, liverworts, and hornworts particularly in Indonesia. Furthermore, these prospective utilization encourage the innovative research in Indonesia, particularly on alternative natural resources.
Plant Species Composition and Their Conspecific Association in Natural Tropical Rainforest, South Papua Murdjoko, Agustinus; Marsono, Djoko; Sadono, Ronggo; Hadisusanto, Suwarno
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 1 (2016): March 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i1.5217

Abstract

Papua has so many wide lowland areas that cover high diversity in plant from life-forms. In contrast, there is a lack of information concerning species diversity. The objectives of the research were to describe species of plant life-forms and described conspecific associations between small individuals and large individuals in natural tropical rainforest, south Papua. Then, 46 nested plots were placed systematically in natural tropical rainforest of Boven Digoel Regency, Papua. Density, frequency, dominance and importance value index (IVI) were analyzed to describe plant diversity, while detrended correspondence analysis (DCA) was to describe conspecific association between small individuals and large individuals. Results found 2040 individuals in this forest consisting 194 plant species. Those are from pteridophytes, angiosperms and gymnosperms. The plant life-forms are herbs, orchids, palms, pandans, shrubs, rattans, vines and trees in which of the plant life-forms, trees are the most abundant according to number of species. In the dominant plant species, two types of conspecific association occurred between small individuals and large individuals. Analysis of DCA revealed that three are large plant species grow along with their small individuals. Another type is that small individuals grow far from their large individuals.
Kesintasan Undur-Undur pada Media Aklimatisasi Arundina, Mayang; Ngabekti, Sri; Santoso, Kukuh
Biosaintifika: Journal of Biology & Biology Education Vol 6, No 1 (2014): March 2014
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v6i1.2930

Abstract

Penelitian ini bertujuan untuk mengetahui kesintasan undur-undur pada media aklimatisasi. Penelitian dilakukan di rumah teduh Jalan Tumpang I/82 Semarang dan Laboratorium Jurusan Biologi FMIPA UNNES. Variabel utamanya kesintasan undur-undur, berat dan panjang undur-undur, sedangkan variabel pendukungnya jenis media aklimatisasi, meliputi tanah kering, abu dan serbuk batu bata. Tahap awal, disiapkan media hidup undur-undur, media kemudian dimasukkan kedalam kotak aklimatisasi, diberi cangkup dan dibiarkan selama 10 hari. Undur-undur ditimbang dan diukur panjang tubuhnya kemudian dimasukkan kedalam kotak aklimatisasi, masing-masing kotak diisi15 undur-undur. Kesintasan hidup undur-undur pada masing-masing media diamati selama 45 hari, data diambil seminggu sekali. Undur-undur diberi makan 2 - 3 ekor semut sehari 3 kali. Setelah 45 hari undur-undur ditimbang dan diukur panjang tubuhnya kembali. Data kesintasan hidup undur-undur dianalisis menggunakan Anava Dua jalur. Berat dan panjang undur-undur dianalisis menggunakan t-test. Hasil penelitian menunjukkan undur-undur dapat sintas pada media aklimatisasi, yaitu tanah kering dan abu. Undur-undur mengalami pertambahan berat dan panjang sesudah penelitian. Berdasarkan hasil penelitian dapat disimpulkan bahwa undur-undurdapat sintas pada media aklimatisasi yaitu tanah kering dan abu. Ukuran berat dan panjang undur-undur mengalami pertambahan sesudah penelitian.This research was aimed to know the survival of gratus at the acclimatization media. The research was held in the shady house at Jalan Tumpang I/82 Semarang and in the Laboratory of Biology Departement of FMIPA UNNES. The main variables were the survival of gratus, the weight and length of gratus, whereas the supporting variable was the acclimatization media, that is the dry sandy soil, the dust and the powder of bricks. Initially, the acclimatization media was prepared for the gratus, then the media was distributed into the boxes of acclimatization media and the box was covered and left unharmed for about 10 days. The gratus were weighed and the body length was measured, and the animals were placed into the boxes of acclimatization media, each box was filled with 15 gratus animals. The survival of gratus in each media was observed for 45 days, the data was taken once a week. The gratus were fed with 2-3 ants at 3 times a day. After 45 days the gratus were weighed and the body length were measured. The data of the gratus survival was analyzed using Tow-Way Anova. The weigh and the body length were analyzed using t-test. It was concluded that gratus can survive in the dry sandy soil and in the dust. The weight and body length were increased during the research
Genetic Variation in Cytochrome b-Hinf1 and -Alu1 Gene Correlated to Body Size in Soang Gourami (Osphronemus goramy) from Single Spawning Kusbiyanto, Kusbiyanto; Soedibja, Petrus Hary Tjahja; Nuryanto, Agus; Qonita, Nael Huda; Pramono, Hendro
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 2 (2017): August 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i2.9301

Abstract

Soang gourami fingerling shows variable body sizes eventhough resulted from single spawning. Differences in body sizes among individuals is assumed to be correlated to their genetic component which can be studied using cytochrome b gene PCR-RFLP marker. This study aimed to determine specific PCR-RFLP marker among different sizes of soang gourami collected from single spawning. Genomic DNA was isolated using Chelex method. Cytochrome b gene were amplified and digested using four restriction enzymes. Specific markers were analyzed descriptivelly based on DNA band pattern appear in agarose gel. The result showed that PCR-RFLP markers of Cytochrome b-HinfI of 315 bp, and 210 bp, and also Cytochrome b-AluI of 334 bp and 189 bp are specific markers for large individuals, whereas small individuals are characterized by having Cytochrome b- HinfI 366 bp, and 159 bp and Cytochrome b-AluI 525 bp fragments. It is observed that genetic variation of Cytochrome b-HinfI and -AluI markers are possitively correlated to body size in soang gourami fingerling. Therefore, both cytochrome b-HinfI and -AluI gene can be reffered as specific markers to differentiate among different sizes of soang gourami strain fingerling from single spawning. This result proved that genetic divergences among individuals can be related with certain quantitative characters, such size related. Therefore our study can contribute on fisheries development, especially by providing new technique for fingerling selection to obtain high quality fingerling and also provide new insight the application of molecular technique in fisheries.
Evaluation of Soybean Resistance to Pod-Sucking Bug, Riptortus linearis F. and Performance of its Agronomic Characters Krisnawati, Ayda; Adie, Mochammad Muchlish
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.12806

Abstract

Pod sucking bug, Riptortus linearis, is the most damaging soybean pest in Indonesia. A total of 24 soybean genotypes was identified for their resistance to pod sucking bug. The objective of the study was to classifying the resistance of some soybean genotypes against pod sucking pests. The research was conducted during the second dry season (July - October) 2016 in Ngale Research Station, East Java, Indonesia. The experimental design was arranged in randomized block design with five times repetition and two types of environment (full crop protection and controlled until 50 days of planting/dap). The resistance to pod sucking bug was evaluated based on seed damage, pod damage, and seed weight. The grouping of resistance was by using Chiang & Talekar’s method (1980). A very high natural population of pod sucking bug was recorded in the research area as seen from the average number of damaged pod in full protection environment (L1) and insecticide control until 50 dap (L2), i.e. 41.45% and 60.16%, respectively. Genotype of G511H/Anj//Anj-2-8 was consistently resistant to pod sucking bug in L1 as well as L2. This genotype also had early days to maturity (78 days) and large seed size (15.57 g/100 seed), thus potentially to be developed in tropical area of Indonesia. The availability of genotype with such characteristics is in accordance with farmers’ preference and also important to minimize yield losses.
Pertumbuhan dan Produksi Kubis Bunga (Brassica oleracea var botrytis) dengan Sistem Pertanian Organik di Dataran Rendah Widiatningrum, Talitha; Pukan, Krispinus Kedati
Biosaintifika: Journal of Biology & Biology Education Vol 2, No 2 (2010): September 2010
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v2i2.1159

Abstract

The research aimed to compare caulifl ower growth and production planted at lowland and highland by using organic farming system. The research was single treatment experimentally, which was land height, consisted of highland (Kopeng) and lowland (Ambarawa). The variables were vegetative growth (leaves amount and plant weight) and generative development (fl ower initiation and fl ower weight). The data were analyzed by t-test. The result confi rmed that organic farming will make better lowland caulifl ower vegetative growth but least generative development compare to the highland one. It can be concluded that caulifl ower have the potency of being growth at lowland, even though with least yield.Keywords: lowland, highland, caulifl ower, organic
Assessing Students’ Ethnicities and Critical Thinking Skill to Develop PBL Based-Biology Learning Tools Boleng, Didimus Tanah; Lumowa, Sonja V.T.; Palenewen, Evie
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 1 (2018): April 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i1.10554

Abstract

A survey has been conducted in grade XI natural science of senior high school, at odd semester, academic year 2016/2017 in Samarinda, Indonesia. The focus of this research are to determine the students’ ethnicities, and the responses of biology teachers about students critical thinking skills, and Problem-Based Learning. Sampling technique was total sampling. The research instrument was a questionnaire. Data was analysed by techniques used descriptive. The results of the data analysis show that the compositions of students’ ethnics are: Java (12.7%), Bugis (6.9%), Kutai (13.3%), Banjar (13.3%), and other ethnics (28.5%). Most of the teachers (86.0%) who already understood about the learning approaches, said that they already understood, while the remaining 14% did not understand the patterns of Problem-Based Learning. In addition, 23.3% teachers said that students were less able to explain, express opinions, and make conclusions; 76.7% said that students are sufficiently able to explain, express opinions, and make conclusions. Future research related to biology learning in multiethnic students needs to be conducted to get more information. The design of learning tools needs to consider the ethnic  of the students in strengthening critical thinking skills. Similar research needs to be done to obtain more information.The findings of this research are the students’ ethnicities of the XI class of Natural Sciences, as well as the responses of high school biology teachers about students critical thinking skills in Biology.
The Effect of Annona Muricata Leaves Towards Blood Levels of Cxcl9 and Lymphoblast (Study in Cerebral Malaria Phase of Swiss Mice) Gadalla, Mohamed M.Y.; Dharmana, Edi; Jamiatun, Kiss; Laksono, Budi
Biosaintifika: Journal of Biology & Biology Education Vol 7, No 2 (2015): September 2015
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v7i2.4078

Abstract

Cerebral malaria (CM) forms part of the spectrum of severe malaria, with a case fatality rate ranging from 15% in adults in southeast Asia to 8.5% in children in Africa. A.Muricata was used to cure Malaria in traditional medicine. The research will examine the effect of it in the chemokine (C-X-C motif) receptor 3 (CXCR3) binding chemokines, including chemokine (C-X-C motif) ligand 4 (CXCL4), CXCL9. The intervented mice group were infected then the its spleen were cultured , incubation 72 hours and then analyzed the result. The CXCL9 level of PbA-infected mice treated with A. muricata are lower than group of infected mice without treatment. Lymphoblast level of PbA-infected mice treated with A. Muricata are higher than group of infected mice without treatment. A. Muricata treatment cure in the CM in the mice and may be a potential treatment in human CM.Cerebral malaria (CM) adalah keadaan infeksi malaria yang berat dengan tingkat kefatalan dari 15% di Asia tenggara dan 8% di Afrika. A. Muricata secara tradisional dipakai mengobati CM. Riset ini meneliti pengaruh A. Muricata pada ikatan chemokine (C-X-C motif) reseptor 3 (CXCR3)termasuk chemokine (C-X-C motif) ligand 4 (CXCL4) dan CXCL9. Kelompok mice intervensi diinfeksi dan limfanya di culture dalam inkubator 72 jam untuk dianalisis. Kadar PbA CXCL9 pada mencit intervensi yang diberi A. Muricata lebih rendah dari pada kontrol. Kadar PbA limfoblast intervensi lebihtinggi dari pada kontrol. A. Muricata memperbaiki CM pada mencit dan berpotensi sebagai pengobat pada CM manusia.

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