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Acta Biochimica Indonesiana
Published by Perhimpunan Biokimia dan Biologi Molekuler Indonesia
ISSN : 26546108     EISSN : 26543222     DOI : https://doi.org/10.32889
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Acta Biochimica Indonesiana (ActaBiolna) is a peer-reviewed and open-access journal that disseminates original research articles and review articles covering diverse topics in Biochemistry and Molecular Biology. The journal is published biannually by Indonesian Society for Biochemistry and Molecular Biology.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 68 Documents
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Antioxidant and antibacterial activity of mangrove Brugueira gymnorrhiza stem extracts against pathogenic bacteria Vibrio cholerae Angga Crystal Loasana Yami; Irmanida Batubara; Kholis Abdurachim Audah
Acta Biochimica Indonesiana Vol. 3 No. 2 (2020): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.v3i2.51

Abstract

Background : The treatment of some diseases caused by free radicals and pathogenic bacteria usually by using antioxidants and antibiotics. Due to excessive use of antibiotics and other environmental cues, some bacteria are now resistant to certain antibiotics or even to multiple antibiotics. Some Vibrio cholerae bacterial strains are multiresistant to many antibiotics. Objective : The antioxidant and antibacterial activities of Brugueira gymnorrhiza stem extracts against pathogenic bacteria V. cholerae. Method : The B. gymnorrhiza stem was extracted by gradient maceration method. The DPPH method was used to determine the antioxidant activity and the disc diffusion method was used to determine the antibacterial activities. The column chromatography method was used to fractionate the selective extract with the best activity. The LC-MS/MS method was used to identify the compound obtained from the fraction with the best antioxidant and antibacterial activity. Result : Ethyl acetate extract of B. gymnorrhiza stem had the best antibacterial activity with MIC and MBC values of 62.50 mg/L. Ethyl acetate extract also showed the best value of antioxidant activity as indicated by an IC50 value of 255.03 mg/L. The results of fractions test showed that fraction 3 had the best antibacterial and the best antioxidant activities with both the MIC and MBC values of 7.90 mg/L and IC50 value of 348.91 mg/L, respectively. Conclusion : Ethyl acetate extract of B. gymnorrhiza stem has good potential as antioxidant and antibacterial. The compound which is thought as antioxidant and antibacterial from Ethyl acetate extract is 2-Ethyl-4-methyl-1H-imidazole.
Subacute toxicity test of Rhizophora apiculata bark extract on liver and pancreas histopathology of rats Syazili Mustofa; Isnamurti Ciptaningrum; Caesaria Sinta Zuya
Acta Biochimica Indonesiana Vol. 3 No. 2 (2020): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.v3i2.52

Abstract

Background: Rhizophora apiculata, one of the mangrove plant widely spread in Indonesia, can be developed as a medicinal plant. The extract of the bark has been found to have antioxidant and anti-inflammatory. However, the toxicity of Rhizophora apiculata has not been established yet. Objective: This research aims to evaluate the toxicity of ethanolic extract of Rhizophora apiculata bark on histopathological changes in rat’s liver and pancreas. Methods: Subacute toxicity study of the ethanol extract of Rhizophora apiculata bark was performed in healthy male rats by administering the extract at doses of 57, 114, 228, 456, and 918 mg/kg of body weight daily for 28 days. The subacute toxicity in rats was determined by histological analyses. Results: No significant adverse effect of the extract at dose 57 mg/kg was found. However at and over 114 mg/kg dose of the extract exhibited toxicities to the rats’ liver. In addition, the toxic effect appeared in rats’ pancreas at and over 228 mg/kg dose. Conclusions: Rhizophora apiculata bark extract showed no toxicity at or below 57 mg/kg. The ethanol extract from bark of Rhizophora apiculata showed toxicity at 114 mg/kg by subchronic toxicity.
The protective effects of red beetroot (Beta vulgaris l.) against oxidative stress in rats induced by high fat and fructose diet Dianandha Septiana Rubi; Abrory Agus Cahya Pramana; Sunarti
Acta Biochimica Indonesiana Vol. 3 No. 2 (2020): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.v3i2.53

Abstract

Background: One of consequence high-fat and fructose diet is oxidative stress. Consumption of antioxidants from red beetroot may increase antioxidant defense. Objectives: This study aimed to evaluate red beetroot administration on improving antioxidant defense in rats induced high fat and fructose diet. Methods: A total 20 male Wistar rats were divided into 4 groups: 1) normal control group (N), received standard diet; 2) High fat and fructose diet (HF), received high fat and fructose diet (HFFD); 8 weeks induction with HFFD and received 9g red beetroot (BA); and combination of HFFD and 9g of red beetroot from beginning of the study (HFBA). At the end of the study the levels of circulatory oxidized LDL (ox-LDL) were determined using enzyme-linked immunosorbent assay (ELISA) method. Superoxide dismutase 2 (SOD2) and catalase (CAT) gene expressions were determined by quantitative polymerase chain reaction (qPCR) method. Results: Induction HFFD increased the levels of circulatory ox-LDL levels compared to normal control (10.00±0.29 vs 12.69±0.57). Administration of red beetroot for 6 weeks and combination HFFD with red beetroot during the study significantly decreased ox-LDL levels compared to high fat and fructose group (12.69±0.57 vs 9.66±0.46) and (12.69±0.57 vs 8.59±0.18), respectively. The decreased circulatory ox-LDL levels were found negatively correlated with upregulation SOD2 (r=-0.548; P=0.012) and CAT (r=-0.460; P=0.041) gene expression in the liver tissues. Conclusion: Administration of red beetroot may ameliorate oxidative stress in rats induced high-fat and fructose diet through increasing antioxidant defense.
Antioxidant activity of red rose leaves (Rosa chinensis jacq.) extract Deviyanthi Nur Afifah; Yessy; Farida Nur Rahmani; Ayu Lia Ningsih; Citra Dewi Puspita Sari; Resna Reformasi Yudapradja; Praptiwi; Rahmawati Ridwan
Acta Biochimica Indonesiana Vol. 3 No. 2 (2020): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.v3i2.56

Abstract

Background: Background: Antioxidants are compounds that can neutralize free radical reactions. The red rose leaves (Rosa chinensis Jacq.), usually disposed of as waste, have been shown to have antioxidant activity. Objective: This study aims to determine the antioxidant activity of red rose leaves extracts. Methods: The red rose leaves were extracted by the maceration method in ethanol, methanol, ethyl acetate, and n-hexane. The antioxidant activity was measured by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and was expressed as IC50 and an antioxidant activity index (AAI). Results: The results showed that the red rose leaves ethanol, methanol, ethyl acetate, and n-hexane extracts had an antioxidant activity with an IC50 value of 1.8, 8.7, 210.3, 48.7 µg/mL, respectively, and an AAI value of 17.3, 3.5, 0.1, 0.6, respectively. The main content of ethanol and methanol extracts are flavonoid, saponin, and tannin. Conclusion: The ethanol and methanol extract showed the most potent antioxidant activity.
Cytotoxic activity and selectivity index of binahong (Anredera cordifolia) extracts on MCF-7 breast cancer cells and vero normal cells line Dita Sozianty; Rifki Febriansah
Acta Biochimica Indonesiana Vol. 3 No. 2 (2020): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.v3i2.57

Abstract

Background: Breast cancers occur because of an impaired balance between proliferation, differentiation, and apoptosis of breast glands. Natural products have potency as cytotoxic agents with less side effects than chemotherapy. One of the potential plants is Anredera cordifolia (Ten.) Stennis (binahong), which contains flavonoid 8-glucopyranosyl- 4’,5,7-trihydroxy flavone compounds. Objective: This study aims to determine the potency of binahong leaves extract as an anticancer for breast cancer in vitro and in silico. Methods: Preliminary tests using molecular docking of 8-glucopyranosyl-4’,5,7-trihydroxyflavone compounds on Bcl-2 and HER-2 proteins. The extraction and fractionation were to obtain binahong extract. Thin layer chromatography to identify flavonoid compounds in the extract. DPPH assay was performed to evaluate the antioxidant activity. MTT assay was performed to evaluate cytotoxic activity on MCF-7 breast cancer cells and Vero cells. Results: In silico test showed a stable bond between 8-glucopyranosyl- 4’,5,7-trihydroxylflavone, and Bcl-2 and HER-2 with a docking score of -7.5 kcal/mol and -8.0 kcal/mol, respectively. The binahong extract contain flavonoid compounds that had the retention factor value 0.78; 0.49; 0.35. Antioxidant test resulted IC50 value of 4940 μg/mL. Cytotoxic test resulted in IC50 value of 1073 μg/mL and 486 μg/mL for Vero cells and MCF-7 breast cancer cells, respectively. The comparison between IC50 produced a selectivity index value of 2.149, which shows that binahong extract was selective against MCF-7 breast cancers. Conclusion: This study concluded that binahong extract has weak potency as anticancer agent on MCF-7 cells.
The persistence of covid-19 infection, a golden opportunity to be observed Fajri Marindra Siregar; Sarmoko
Acta Biochimica Indonesiana Vol. 3 No. 2 (2020): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.v3i2.62

Abstract

Increased level of malondialdehyde in preterm labor Yesi Mustika Sari; Eti Yerizel
Acta Biochimica Indonesiana Vol. 4 No. 1 (2021): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.8

Abstract

Background: The pathophysiological mechanism associated with spontaneous preterm delivery is oxidative stress through the increased formation of reactive oxygen species (ROS) due to lipid peroxidation. Malondialdehyde (MDA) is one of the biomarkers of oxidative stress produced through the lipid peroxidation process. Objective: The aim of this study is to observe the difference in MDA levels among preterm labor compared to full-term labor. Methods: Observational research was conducted with a comparative cross-sectional design. Maternal venous blood samples were taken from private hospitals and midwives in Padang city and Aro Suka Hospital Solok Regency. Samples were selected by consecutive sampling and divided into two groups with a total of 40 samples. MDA level was measured using the spectrophotometry method. Results: MDA levels in preterm delivery were 3,6±0.42 nmol/mL and in full-term delivery were 2.9±0.33 nmol/mL. Conclusion: There was a significant difference in MDA levels between preterm labor and full-term delivery. MDA levels in preterm childbirth were higher than MDA levels in full-term delivery.
Antibacterial activity of betel (Piper betle L.) fruit against Pseudomonas aeruginosa Annisa Hamka; Fatimawali Fatimawali; Olvie Syenni Datu; Trina Ekawati Tallei
Acta Biochimica Indonesiana Vol. 4 No. 1 (2021): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.10

Abstract

Background: Betel (Piper betle L.) is a well-known medicinal plant for its numerous health benefits. Saponins, flavonoids, polyphenols, and essential oils are among the chemical constituents of betel plants. Flavonoids are one of the most common groups of secondary metabolites found in plant tissues, including in betel plants. Objective: The purpose of this research is to isolate flavonoids from betel fruit and to determine the antibacterial activity of betel fruit extract against Pseudomonas aeruginosa. Methods: Thin-layer chromatography (TLC) with the eluent chloroform: methanol: water was used to isolate flavonoids. UV-Vis spectrophotometry was used to determine the presence of flavonoids in betel fruit. The antibacterial activity of extract and TLC-isolates of betel fruit was tested by using the disc method. Results: TLC analysis resulted in the formation of a brown stain. The UV-Vis spectrophotometry results revealed two absorption bands at 366 nm and 268 nm, indicating that flavonoids are present in betel fruit. Antibacterial activity test against Pseudomonas aeruginosa bacteria showed that the concentration of 30% and 60% of betel fruit extract had strong antibacterial activity. Conclusion: The results revealed that the betel fruit contains flavonoid compounds, and the extract has medium to strong antibacterial activity.
Genetic polymorphism of ALDH2 in Indonesia’s Minang ethnic Abdul Halim Sadikin; Irene Dian; Mukharjon Mukharjon; Rini Puspitaningtum; Septelia Inawati Wanandi
Acta Biochimica Indonesiana Vol. 4 No. 2 (2021): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.14

Abstract

Background: In some people, acetaldehyde, a toxic product from ethanol oxidation, cannot be oxidized to acetate. The excess of acetaldehyde could cause facial flushing, dizziness, and hypertension when they consume ethanol. This ethanol sensitivity is caused by a deficiency of ALDH2. Objective: This study aims to analyze and count the polymorphism frequency of the ALDH2 gene in Indonesia’s Minang ethnic. Methods: DNA samples were taken randomly from hair bulbous of 60 subjects (male and female, 3rd generation). A nested polymerase chain reaction was conducted to amplify the ALDH2 in the samples. Afterward, restriction fragment length polymorphism (RFLP) was conducted to the amplicons using the EcoRI restriction enzyme. The measured parameters were the distribution of the wildtype, atypical homozygote, and heterozygote. Results: Results showed that out of 60 subjects, 53.33% have an atypical homozygote gene (subjects prone to hypersensitive to alcohol), 28.33% have a heterozygote gene, and 18.33% have a wildtype gene. The frequency of the atypical alleles in Minang ethnic is 0.675. Conclusion: The atypical ALDH2 allele was much higher than the normal ALDH2 allele, in which most participants have atypical homozygote ALDH2, suggesting the samples are sensitive to alcohol.
The relationship between vitamin E and C intake with total activity of erythrocytes and breast milk superoxide dismutase in lactating mothers Ninik Mudjihartini; Diyah Eka Andayani; Sheira Taflah Putri Handana
Acta Biochimica Indonesiana Vol. 4 No. 2 (2021): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.15

Abstract

Background: Human milk contains many components, one of them is superoxide dismutase (SOD). Vitamin E and C, together with SOD, can prevent oxidative stress. Objective: This study investigated the correlation between vitamin E and vitamin C intake, with total SOD activity, in erythrocyte and breast milk among lactating mothers in Jakarta, Indonesia. Methods: Sixty lactating mothers aged 20–40 years were recruited in 1–6 months postpartum in Grogol Petamburan and the Cilincing Public Health Centre from March 2019 until April 2019. Vitamins E and C dietary intake were collected using a semi-quantitative food frequency questionnaire. SOD total activity of erythrocyte and breast milk was measured using the Ransod kit 125. Results: The median value of vitamin E intake was 6.50 mg/day, showing 91.7% of patients do not meet recommended daily intake (RDA) (19 gram/day), and the median of vitamin C intake was 120.05 mg/day with 70% participants fulfilling RDA. SOD total activity in erythrocyte and breastmilk showed a median value of 423.73 U/mL and 58.34 U/mL, respectively. The correlation between vitamin E intake with total SOD activity in erythrocyte (r = 0.143 p > 0,05) and breast milk (r = 0.041, p > 0,05) was not significant. Vitamin C intake was also not significantly correlated with SOD total activity in the erythrocyte. Conclusion: There is no significant correlation between vitamin E and vitamin C intake with the total activity of SOD of erythrocyte and breast milk in lactating mothers.

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