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HAYATI Journal of Biosciences

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Published by Institut Pertanian Bogor

ISSN : 19783019 EISSN : 20864094 DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences

HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, animal and human. HAYATI J Biosci has been also indexed/registered in Crossref, DOAJ, CABI, EBSCO, Agricola and ProQuest.

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Articles 10 Documents

Search results for , issue "Vol. 17 No. 1 (2010): March 2010" : 10 Documents clear

Intracellular Ca2+ Regulation in Calcium Sensitive Phenotype of Saccharomyces cerevisiae . HERMANSYAH
HAYATI Journal of Biosciences Vol. 17 No. 1 (2010): March 2010
Publisher : Bogor Agricultural University, Indonesia

Intracellular cytosolic Ca2+ concentration accumulation plays an essential information in Saccharomyces cerevisiae i.e. to explain cellular mechanism of Ca2+ sensitive phenotype. Disruption both S. cerevisiae PPase PTP2 and MSG5 genes showed an inhibited growth in the presence of Ca2+. On the other hand, by using Luminocounter with apoaequorin system, a method based upon luminescent photoprotein aequorin, intracellular Ca2+ concentration was accumulated as a consequence of calcium sensitive phenotype of S. cerevisiae. This fact indicated that PPase ptp2D and msg5D were involved in intracellular Ca2+ transport in addition their already known pathways i.e Mitogen Activated Protein Kinase cell wall integrity pathway, high osmolarity glycerol (HOG) pathway, and pheromone response FUS3 pathway.

Control of Fusarium Wilt of Chili With Chitinolytic Bacteria Dwi Suryanto; Siti Patotah; Erman Munir
HAYATI Journal of Biosciences Vol. 17 No. 1 (2010): March 2010
Publisher : Bogor Agricultural University, Indonesia

Biological control of plant disease using antagonistic microorganism has been obtaining much attention and implemented for decades. One of the potential microorganisms used in this strategy is chitinolytic bacteria. Utilization of this bacteria ranges from cell life, enzymes, genes, or other metabolites. In this study, we examined the ability of chitinolytic bacteria as a biocontrol agent of Fusarium wilt of red chili (Capsicum annuum L.) seedlings. The ability of chitinolytic bacteria to suppress the disease was evaluated by soaking red chili seeds in the bacterial isolates solution for 30 minutes prior seedling. Percentage of seedling of treated chili seed at end of study (4-weeks) ranging from 46 to 82.14%. Relative reduction of the seedling damping-off was observed in all bacterial treatment ranged from 28.57 to 60.71%. Furthermore, manifestation of bacterial suppression to Fusarium wilt was also exhibited by increasing of seedling height (ranged from 7.33 to 7.87 cm compared to 6.88 cm) and dry-weight (ranged from 2.7 to 4.3 mg compared to 2.3 mg). However, no significant effect was observed in leaf number. Then, from all chitinolytic isolates tested, BK08 was the most potential candidate for biological control agent of Fusarium wilt in chili seedling.

Phylogenetic Study of Mangifera laurina and its Related Species Using cpDNA trnL-F Spacer Markers . Fitmawati; Alex Hartana
HAYATI Journal of Biosciences Vol. 17 No. 1 (2010): March 2010
Publisher : Bogor Agricultural University, Indonesia

Phylogenetic study of cpDNA intergenic spacer trnL-F of Mangifera laurina and their related species within the genus Mangifera in Indonesia was conducted using Rutaceae as the outgroup. This study was to reconstruct phylogenetic relationships and to understand infraspecific relationships within Mangifera based on cpDNA trnL-F intergenic spacer sequences. The results showed that Mangifera sp. Hiku (mangga hiku) as the basic cultivar in the clade, and it supported the monophyletic group in Mangifera. And phylogenetic construction indicated that Mangifera sp. Hiku was the progenitor of M. laurina and their related species.

Differential Regulation of the Nodulation Zone by Silver Ions, L--(2-Amino-Ethoxyvinyl)-Glycine, and the skl Mutation in Medicago truncatula Joko Prayitno; Ulrike Mathesius
HAYATI Journal of Biosciences Vol. 17 No. 1 (2010): March 2010
Publisher : Bogor Agricultural University, Indonesia

Nodule formation in Rhizobium-legume symbiosis is negatively regulated by ethylene. Ethylene inhibitors such as L-a-(2-amino-ethoxyvinyl)-glycine (AVG) and silver ions (Ag+), the ethylene-insensitive sickle mutant, and transgenic plants were used to study ethylene-mediated responses in nodulation. The mode of action of ethylene inhibitors AVG and Ag+, and the skl mutation occur at different steps in ethylene biosynthesis and perception. Their effects on root growth and nodulation phenotypes, in particular nodule distribution along the primary root, were compared in this study. Ag+ and AVG treatments showed similar root growth responses to skl mutant. However, nodule distribution in the hypernodulating skl mutant is different from that of wild-type plants grown on either AVG or Ag+. AVG increased nodule numbers and widened the nodulation zone, while the skl mutant had an increased number of nodules within the susceptible zone of nodulation. Ag+ reduced nodule numbers, restricted the nodulation zone, and restored the nodulation phenotype of skl to that of the wild type.

Amplified Fragment Length Polymorphism Diversity of Cultivated White Oyster Mushroom Pleurotus ostreatus Muhammad Jusuf
HAYATI Journal of Biosciences Vol. 17 No. 1 (2010): March 2010
Publisher : Bogor Agricultural University, Indonesia

Amplified fragment length Polymorphism (AFLP) analysis was performed to study the genetic diversity of fifteen isolates of white oyster mushroom (Pleurotus ostreatus) originated from different places in Indonesia (Java, Sumatra, Bali, and Kalimantan) and Thailand. The majority of studied isolates originated from commercial mushroom, and some of them were collected from nature. AFLP analysis revealed 202 loci of positive DNA bands. Heterozygosity value showed polymorphisms of the fifteen studied isolates. Correlation and cluster analyses of the isolates showed that isolate from Thailand (BNK isolate), isolate from Bogor (AMD isolate), and isolate from Purwokerto (USX isolate) were distinctly different from the other isolates indicated by positive bands with DNA size greater than 650 bp. Isolate from Kalimantan (BJM isolate) was distinctly different from Java island isolates. Generally there was no correlation of AFLP variability and geographic distribution of commercial strain isolates.

Quality of Epididymal and Ejaculated Sperms of Spotted Buffalo in Dextrose Supplemented Extender . Yulnawati; Muhammad Gunawan; Hera Maheshwari; Muhammad Rizal; . Herdis; Arief Boediono
HAYATI Journal of Biosciences Vol. 17 No. 1 (2010): March 2010
Publisher : Bogor Agricultural University, Indonesia

Quality of epididymal and ejaculated sperms of spotted buffalo in three different extenders (i.e. Andromed, Andromed supplemented with 0.2% dextrose, and Andromed supplemented with 0.4% dextrose) was studied. The results showed that there was no significantly different (P > 0.05) quality of ejaculated and epididymal sperms. The motility percentage of post thawing epididymal and ejaculated sperms was 41-46% and 41-45%, respectively. And percentage of cytoplasmic membrane integrity of the post thawing epididymal and ejaculated sperms was 66-67% and 47-54% respectively. There was also no significant different (P > 0.05) in the percentage of cytoplasmic membrane integrity between the extenders of both ejaculated and epididymal sperms. This result suggested that epididymal sperms were reliable for artificial insemination as good as ejaculated sperms.

Inhibition of Urokinase-Type Plasminogen Activator Expression by Macelignan in Porphyromonas gingivalis Supernatant-Induced Human Oral Epithelial Cells . Yanti
HAYATI Journal of Biosciences Vol. 17 No. 1 (2010): March 2010
Publisher : Bogor Agricultural University, Indonesia

This study was to investigate the effect of macelignan on Porphyromonas gingivalis supernatant-induced uPA expression via regulating mitogen-activated protein kinase (MAPK) and activating protein-1 (AP-1) signaling pathways in human oral epithelial KB cells using casein zymography, Western blotting, reverse transcription-PCR and reporter gene assays. Zymographic analysis of secreted enzymes identified the main caseinolytic band at 54 kDa. Macelignan inhibited the expression of uPA protein and mRNA, as well uPA secretion, in KB cells exposed to P. gingivalis supernatant. Consistent with these findings, macelignan suppressed phosphorylation of p38 and c-Jun N terminal kinase (JNK) in P. gingivalis supernatant-induced KB cells. The levels of c-Fos and phosphorylated c-Jun, which together form AP-1, the transcription factor that is involved in uPA gene expression, were partially reduced by macelignan. Macelignan also blocked P. gingivalis supernatant-induced AP-1 activity in these cells. These results suggest that macelignan decreased P. gingivalis supernatant-induced uPA expression by blocking AP-1 activity, which may be mediated by inhibition of phosphorylation of p38 and JNK in KB cells. Macelignan may potently use for the modulation of periodontal inflammation.

Diversity and Effectiveness of Insect Pollinators of Jatropha curcas L. (Euphorbiaceae) Puji Rianti; Bambang Suryobroto; Tri Atmowidi
HAYATI Journal of Biosciences Vol. 17 No. 1 (2010): March 2010
Publisher : Bogor Agricultural University, Indonesia

Seed of Jatropha curcas (Euphorbiaceae) is currently established as the source for biofuel Therefore, it is important to understand the diversity insects that pollinated J. curcas inflorescence yellow flowers. We also aimed to study the pollination effects on fruit set on J. curcas. Scan sampling method were carried out to explore the insect pollinators diversity from 07.00 up to 17.00 h in every 15 minutes. Visiting frequency of pollinators insects were observed by using focal sampling. Those information together with flowering periods, flower nectar volumes, and environmental factors were used as the basic data to determine the effectiveness of insect pollination both in covered and uncovered of seed set plants. Results showed that nine species of insect pollinators were from three order (Hymenoptera, Lepidotera, and Diptera) pollinated J. curcas. Four species of Hymenoptera i.e. Prenolepis, Apis dorsata, Xylocopa confusa, and Apis cerana showed the highest abundances. The highest abundance and species richness of pollinators occurred at 08.00-10.15 and 15.00-17.15 h. Bees of X. confusa, A. cerana, and A. dorsata of Apidae are effective as insect pollinators in J. curcas plantations, due to high visited frequencies. The insect pollinators also increased fruits and seeds set of J. curcas in the uncovered experiment plants. Thereby, enhancement the three pollinator insects as part of crop management have to be considered by farmers.

Fecal Steroid Profile of Female Javan Gibbons (Hylobates moloch) Maintained in Pairing-Typed Cage Hera Maheshwari; Luthfiralda Sjahfirdi; Pudji Astuti; Bambang Purwantara; Hadi Sukardi Alikodra; Dondin Sajuthi; Reviany Widjajakusuma
HAYATI Journal of Biosciences Vol. 17 No. 1 (2010): March 2010
Publisher : Bogor Agricultural University, Indonesia

Estrone conjugate (E1C) and pregnanediol glucuronide (PdG) were predominant steroid metabolites of estrogen and progesterone in feces of most primates and could be used to evaluate ovarian function. These metabolites were determined along with records of genital swelling throughout 3-4 months period from three female Javan Gibbons (Hylobates moloch) maintained in pairing-typed cage at Schmutzer Primate Center, Jakarta (Ullah) and at Taman Margasatwa Taman Sari, Bandung (Donna and Citah). Following methanolic extraction of lyophilized fecal powder, samples were analyzed using enzyme linked immunosorbent assay (ELISA) for E1C and PdG. In all of the three females observed, both hormone profiles did not indicate any regular cycle of ovarian function even though genital swellings were sometimes observed. In one female (Donna) the hormone patterns showed clear signs of cycle irregularities with extended luteal phase of 40 days and erratic pattern of follicular phase. Of the other two females, no ovarian cycle was found. The data indicate that the fecal steroids analysis is a practical and valuable diagnostic tool for providing reliable information on ovarian function in Javan Gibbon. Factors affected reproductive hormonal profile should be taken in consideration in trying to achieve success in captive breeding program for this species.

Single Strand Conformation Polymorphism Method for Initial Detection DNA Sequences Homogeneity Topik Hidayat; Adi Pancoro
HAYATI Journal of Biosciences Vol. 17 No. 1 (2010): March 2010
Publisher : Bogor Agricultural University, Indonesia

In molecular phylogenetic study, homogeneity of DNA sequences is a prerequisite before putting it into practice. Internal transcribed spacer (ITS) region of nuclear ribosomal DNA (nrDNA) has been common in phylogenetic study, but a homogenous sequence is often difficult to obtain. Here we use single-stranded conformation polymorphism (SSCP) method to detect homogeneity for nine pooled amplified products of ITS region. Our results suggested that SSCP method has been applicable in detection homogeneity of ITS region prior to using it in sequencing processes.

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