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INDONESIA
Indigenous Biologi
Published by Universitas Kristen Artha Wacana
ISSN : 26144816     EISSN : 26569787     DOI : https://doi.org/10.33323/indigenous
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Jurnal Indigenous Biologi (JIB) adalah jurnal menggunakan double blind peer review dan akses terbuka yang menerbitkan penelitian penting dan penting dari semua bidang bidang biosciences seperti keanekaragaman hayati, biosistematik, ekologi, fisiologi, perilaku, genetika dan bioteknologi. Meliputi semua bentuk kehidupan, mulai dari mikroba, jamur, tumbuhan, hewan, dan manusia, termasuk virus.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 68 Documents
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SELEKSI ENZIM PROTEASE JAMUR ENDOFIT DAUN MANGROVE Avicennia marina DI PANTAI NOELBAKI Ocsryn Lylianti Ludji Lobo
Indigenous Biologi : Jurnal Pendidikan dan Sains Biologi Vol 5 No 3 (2022): Indigenous Biologi
Publisher : Prodi Pendidikan Biologi FKIP Universitas Kristen Artha Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33323/indigenous.v5i3.267

Abstract

ABSTRAK Jamur endofit dapat diartikan sebagai mikroba yang hidup berkoloni dalam jaringan internal makhluk hidup seperti pada daun tanpa menyebabkan efek yang merugikan secara langsung pada organisme tersebut. Jamur endofit mampu menghasilkan enzim ekstraseluler, salah satunya enzim protease. Penelitian ini bertujuan untuk mengidentifikasi jamur endofit penghasil enzim protease yang hidup pada daun mangrove Avicennia marina di Pantai Neolbaki. Metode yang digunakan adalah eksperimental dengan dua perlakuan yaitu daun tua dan daun muda mangrove A. marina. Data karakterisasi morfologi secara makroskopis dan mikroskopis dianalisis secara kualitatif sedangkan data uji aktifitas protease dari jamur endofit dengan metode plug agar berupa hasil perhitungan zona bening secara kuantitatif dan dideskripsikan dalam bentuk gambar dan tabel. Berdasarkan data penelitian ditemukan bahwa dari daun tua dan daun muda mangrove A. marina yang ada di pantai Noelbaki menghasilkan 12 total isolat hasil isolasi. Dari total 12 isolat diperoleh 5 isolat jamur endofit penghasil enzim protease ekstraseluler yang terbagi atas 3 isolat dari daun tua dan 2 isolat dari daun muda. Pembentukan zona aktifitas proteolitik tertinggi pada daun tua A. marina dalam uji enzim protease ekstraseluler terdapat pada kode isolat DT Am 05 OLL dengan diameter zona proteolitik sebesar 2,1 mm. sedangkan untuk daun muda A. marina aktivitas tertinggi terdapat pada kode isolat DM Am 02 OLL dengan diameter zona bening sebesar 1,9 mm. Kata Kunci: Avicennia marina, Enzim protease ekstraseluler, Jamur endofit. ABSTRACT Endophytic fungi can be defined as microbes that live in colonies in the internal tissues of living things such as leaves without causing direct adverse effects on these organisms. Endophytic fungi are able to produce enzymes, one of which is the protease enzymes. This study aims to identify Endophytic fungi that produce protease enzymes that live on mangrove leaves of Avicennia marina in Noelbaki Beach. The research used an experimental method with two treatments, namely old and young leaves of the A. marina mangrove. The macroscopic and microscopic morphological characterization data were analyzed qualitatively, while the protease activity test data of endophytic fungi using the plug agar and the clear zone were analyzed quantitatively, then described in the form of figures and tables. The founding of the research of the old and young leaves of A. marina from the Noelbaki beach had been isolated a total of 12 isolates of endophytic fungi. Of the 12 isolates, 5 isolates were shown to produce extracellular protease enzymes, consisting of 3 old leaves isolates and 2 young leaves isolates. In the extracellular protease enzyme test, the proteolytic activity zone was confirm as a clear zone was found to be the highest in the old leaves of A. marina found in the isolate code DT-Am-05-OLL with a proteolytic zone diameter of 2.1 mm. As for young leaves of A. marina, the highest activity was found in the DM-Am-02-OLL isolate code with a clear zone diameter of 1.9 mm. Key Words : Avicennia marina, endophytic fungi, extracellular protease enzymes
Bakteriuria Asimtomatik Pada Lansia Wanita Di Panti Sosial Tresna Werdha Kota Denpasar Ni Wayan Desi Bintari
Indigenous Biologi : Jurnal Pendidikan dan Sains Biologi Vol 5 No 3 (2022): Indigenous Biologi
Publisher : Prodi Pendidikan Biologi FKIP Universitas Kristen Artha Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33323/indigenous.v5i3.341

Abstract

Asymptomatic bacteriuria is defined as the presence of significant growth of microorganisms in the urinary tract without signs of infection. The prevalence increases in geriatric or elderly patients who live in groups in nursing homes. This study aims to determine the description of asymptomatic bacteriuria in the elderly in one of the nursing homes in Denpasar City. The study was conducted at the Bacteriology Laboratory of STIKes Wira Medika Bali in May-August 2021. The number of samples in this study were 23 elderly women who were selected by purposive sampling technique from 28 populations. Examination of asymptomatic bacteriuria was performed by quantitative culture of urine. Identification of the causative pathogen is carried out by biochemical tests. Quantitative culture of urine culture and identification of bacteria were carried out in two consecutive examinations. The results of asymptomatic bacteriuria examination were obtained 8 samples (34.78%) were positive. According to identification result, pathogenic bacteria causing asymptomatic bacteriuria were E. coli, K. pneumoniae, P. aeruginosa and β hemolytic Streptococcus sp. Based on the results of this study to reduce the risk of asymptomatic bacteriuria in elderly, it is necessary to evaluate personal hygiene and sanitation.
INFLUENCE OF USE OF FIGURE TOOL FROM USED MATERIALS TOWARDS STUDENT LEARNING RESULTS IN SMP NEGERI 1 KUPANG CITY ACADEMIC YEAR 2018/2019 Ance Sayani Riwu Lobo
Indigenous Biologi : Jurnal Pendidikan dan Sains Biologi Vol 5 No 3 (2022): Indigenous Biologi
Publisher : Prodi Pendidikan Biologi FKIP Universitas Kristen Artha Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33323/indigenous.v5i3.76

Abstract

The purpose of this study was to determine the effect of the use of teaching aids from used materials on student learning outcomes. The population of all students in class VIII was 198 students and sample of class VIIIB and class VIIIC totaled 61 students. The method used is an experimental method with the Posstest-Only Control Group Design. The sampling technique used is Cluster Sampling. Data collection is done by learning achievement tests. Student learning outcomes data obtained were analyzed using the t test, with the help of SPSS. Based on the homogeneity test results obtained an F value of 6,560 and the hypothesis test results obtained t value of 11,403 with a significance of 0,000 or less than 0.5 it shows that there are differences in student learning outcomes between the control class and experiment. because by using props students can see directly. This can be seen from the average value of 85.81 compared to student learning outcomes in the control class. Because in the experimental class students more actively find out for themselves about important concepts in learning so that students more easily remember, understand, apply and apply what is found in learning. Thus it can be concluded that H1 is accepted and H0 is rejected, meaning that there is a significant influence in the use of teaching aids on student learning outcomes.
PENGEMBANGAN MODUL PEMBELAJARAN BERPIKIR KRITIS PADA MATERI KHUSUS KEANEKARAGAMAN HAYATI KELAS X IPA SMA 1 CAWANG BARU Nowlinda Kamuihkar; Marina Silalahi; Jendri Mamangkey
Indigenous Biologi : Jurnal Pendidikan dan Sains Biologi Vol 6 No 1 (2023): Indigenous Biologi
Publisher : Prodi Pendidikan Biologi FKIP Universitas Kristen Artha Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33323/indigenous.v6i1.361

Abstract

The implementation of Biology class in a Science Class grade X in SMA 1 Cawang Baru is not optimal yet, particularly in utilizing the learning modules. It makes the learning neglected considering the students’ assignments in the class are clearly intended to listen and record the clarification proposed by teachers. This study aimed to develop a learning model for the topic of Biodiversity by measuring how feasible the module is being used and identifying the students’ responses after using the learning module that had been compiled. The method used in this study was Research and Development (R&D) and it was conducted in SMA 1 Cawang Baru from November 2021 to January 2022 by distributing questionnaires to collect the research data. The findings showed that the module compiled by the teachers was feasible and able to be recommended as a learning module in Senior High Schools, particularly for Science Class grade X. This learning module covers a title page, preface, table of contents, mind map, core competencies, basic competencies, indicators, learning goals, summaries of learning materials, example questions, references, and glossary. The module feasibility was obtained from the expert validation score. Six (6) experts had been involved: two language experts with an average score of 78% in the “feasible” category, two media experts with a score of 87.5% in the “strongly feasible” category, and two learning material experts with a score of 90% in the “strongly feasible” category. Therefore, the overall percentage from all validation experts was 84% in the “strongly feasible” category, indicating that the module is feasible to be used. Meanwhile, based on the students’ responses, the percentage fulfilled the following required aspects: presentation (70%), language (64%), and learning content (69%). Consequently, the average percentage of the students’ responses to the learning module being developed is 69% in the “Good” category.
ANALISIS TOTAL BAKTERI VIBRIO PADA SAMPEL AIR TAMBAK UDANG VANAME DI BALAI PERIKANAN BUDIDAYA AIR PAYAU TAKALAR Syarif Hidayat Amrullah; Khusnul Mar'iyah
Indigenous Biologi : Jurnal Pendidikan dan Sains Biologi Vol 6 No 1 (2023): Indigenous Biologi
Publisher : Prodi Pendidikan Biologi FKIP Universitas Kristen Artha Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33323/indigenous.v6i1.380

Abstract

Vibriosis merupakan suatu penyakit yang sering ditemukan pada budidaya perairan terutama pada udang yang disebabkan oleh bakteri Vibrio sp. yang dapat mengakibatkan kematian pada udang. Penelitian ini bertujuan untuk mengetahui total bakteri vibrio pada air tambak udang vaname dengan menggunakan metode TPC (Total Plate Count) berdasarkan SNI 2332.3:2015. Berdasarkan hasil penelitian, sampel air tambak udang vaname memenuhi persyaratan untuk budidaya udang, yaitu dibawah 104 CFU/mL kecuali pada sampel kode B.565/24/02/22 mengandung bakteri Vibrio sp sebanyak 12.250 CFU/mL. Kepadatan bakteri Vibrio sp yang terlalu tinggi dapat menyebabkan udang rentan terserang penyakit vibriosis.
INVENTARISASI VARIETAS-VARIETAS UBI KAYU (Mannihot esculenta Crantz) DAN UBI JALAR (Ipomoea batatas L ) YANG TERDAPAT DI KABUPATEN TIMOR TENGAH SELATAN angela femina banunaek
Indigenous Biologi : Jurnal Pendidikan dan Sains Biologi Vol 6 No 1 (2023): Indigenous Biologi
Publisher : Prodi Pendidikan Biologi FKIP Universitas Kristen Artha Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33323/indigenous.v6i1.211

Abstract

Provinsi Nusa Tenggara Timur (NTT) merupakan salah satu provinsi yang kaya akan keanekaragaman hayati khususnya pada tanaman pangan. Masyarakat provinsi NTT umumnya mengkonsumsi pangan lokal (jagung, umbi-umbian dan kacang-kacangan) sebagai makanan pokok meskipun saat ini terdapat kecenderungan masyarakat konsumsi beras (Yusuf, 2015). Salah satu tanaman pangan yang dapat memberikan nilai ekonomi dan dapat memenuhi kebutuhan hidup manusia adalah tanaman umbi-umbian khususnya ubi kayu dan ubi jalar. Ubi kayu (Mannihot esculenta Crantz) adalah salah satu umbi-umbian yang dikenal sebagai tanaman perdu yang berumbi, berdaun menjari dan berbuah kotak. Ubi jalar (Ipomoea batatas L) merupakan salah satu dari tanaman umbi-umbian yang tipe pertumbuhannya merambat. Kabupaten Timor Tengah Selatan memiliki banyak tanaman umbi-umbian terkhususnya ubi kayu dan ubi jalar namun, pemahaman masyarakat tentang varietas-varietas ubi kayu dan ubi jalar masih kurang. Salah satu upaya yang dapat dilakukan adalah dengan melakukan inventarisasi dan pendataan kembali tanaman ubi kayu dan ubi jalar yag terdapat di Kabupaten TTS.Tujuan dari penelitian ini adalah untuk mengetahui varietas-varietas ubi kayu dan ubi jalar yang terdapat di Kabupaten TTS dan untuk mengetahui karakteristik morfologi dari setiap varietas tanaman ubi kayu dan ubi jalar yang ditemukan di Kabupaten TTS. Penelitian ini menggunakan metode jelajah dan melakukan wawancara. Analisis data menggunakan deskriptif kualitatif. Hasil penelitain ini ditemukan 7 varietas ubi kayu yaitu varietas Darul hidayah, Adira 1, UK1 Agritan, Malang 1, Malang 2, Malang 6 dan varietas Litbang UK-2 dan 8 varietas ubi jalar, yaitu varietas Cilembu, Papua solossa, Muara takus, Antin 1, Antin 2, Kalasan, Boko dan varietas SawentarKata Kunci : Inventarisasi, Ubi kayu, Ubi jalar
ANALISIS STRUKTUR MORFOLOGI, ANATOMI EKSTREMITAS, DAN PERILAKU MONYET EKOR PANJANG (Macaca fascicularis) DI OBYEK WISATA GOA KREO SEMARANG Lena Enjelina; Bunga Ihda Norra
Indigenous Biologi : Jurnal Pendidikan dan Sains Biologi Vol 6 No 1 (2023): Indigenous Biologi
Publisher : Prodi Pendidikan Biologi FKIP Universitas Kristen Artha Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33323/indigenous.v6i1.358

Abstract

Objek Wisata Goa Kreo Semarang merupakan tempat wisata edukasi karena di dalamnya di huni oleh ratusan monyet ekor panjang yang memiliki morfologi dan perilaku yang berbeda pada setiap individu, serta anatomi extremitas yang memiliki kemiripan dengan manusia. Penelitian ini bertujuan untuk mengetahui morfologi dan perilaku dari monyet ekor panjang (Macaca fascicularis) dan sistem tubuhnya. Desain penelitian berupa kualitatif deskriptif. Teknik pengumpulan data menggunakan metode observasi dan kajian literatur dari berbagai artikel jurnal serta buku. Kegiatan observasi meliputi melakukan pencatatan karakteristik struktur morfologi, perilaku, dan habitat dari monyet ekor panjang (Macaca fascicularis), serta pengambilan dokumentasi. Studi literatur meliputi analisis dari data anatomi dan sistem tubuh monyet ekor panjang. Hasil penelitian menunjukkan bahwa monyet ekor panjang (Macaca fascicularis) jantan dan betina memiliki karakteristik morfologi dan perilaku yang berbeda. Monyet jantan memiliki tubuh lebih panjang dan besar, memiliki penis, terdapat rambut menyerupai kumis pada pipi, ekor lebih panjang dan kepala lebih bulat, mobilitas tinggi, sering berkelahi, grooming, dan mendominasi. Monyet betina memiliki tubuh lebih ramping, tidak ada alat kopulasi, memiliki rambut menyerupai jenggot pada dagunya, memiliki kelenjar susu, tidak banyak beraktivitas, lebih banyak duduk, beristirahat, dan menggendong anaknya. Ciri khas anatomi berupa kantong pipi (cheek pouch). Mobilitas monyet ekor panjang berdasarkan waktu.
Formulasi Bioinsektisida Berbahan Aktif Metarrhizium anisopliae Isolat Lokal untuk Mengendalikan Hama Cylas formicarius pada Ubi Jalar Nina Jeni Lapinangga
Indigenous Biologi : Jurnal Pendidikan dan Sains Biologi Vol 6 No 1 (2023): Indigenous Biologi
Publisher : Prodi Pendidikan Biologi FKIP Universitas Kristen Artha Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33323/indigenous.v6i1.407

Abstract

One of the limiting factors for sweet potato productivity in the South Central Timor District is the Cylas formicarius pest attack. This control using synthetic pesticides can have a negative impact. One alternative pest control that can reduce these impacts is biological pest control. The use of bioinsecticides from entomopathogenic fungi on a wide scale needs to pay attention to the right formulation that can help in terms of availability, mass propagation, storage and application. This study aims to find an appropriate and effective formulation of Metarhizium anisopliae for mass production. The results of this study were a local isolate of the fungus M. anisopliae which was enriched with insect meal with a high virulence level. There are three types of formulations made and tested, namely pellet, flour, and liquid formulations. This study was arranged using a completely randomized design with 4 treatments and 4 replications. Data on fungal growth and pathogenicity were tested by means of variance and followed by the Least Significant Difference test at the 5% level. The results showed that the density of the conidia of M. anisopliae in the liquid formulation was significantly higher (10.31 x 109 /ml) compared to the alginate pellet formulation (8.65 x 109 /ml) and the flour formulation (8.65 x 109 /ml). The highest mortality was found in liquid formulations. This means that the liquid bioinsecticide made from M. anisopliae is the most virulent against C. formicarius larvae. Keywords: bioinsecticide formulation, entomopathogenic fungus, Metarhizium anisopliae.

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