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Journal of Indonesian Medical Laboratory and Science (JoIMedLabS)
Published by Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medis Indonesia
ISSN : 27750108     EISSN : 27742504     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology
Journal of Indonesian Medical Laboratory and Science (JoIMedLabS) merupakan jurnal AIPTLMI (Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia) yang dapat diakses secara terbuka (open acces) yang berfokus pada pengembangan ilmu pengetahuan Teknologi Laboratorium Medik (TLM) dengan tujuan untuk memperluas pengetahuan, pemikiran dan penelitian di bidang laboratorium kesehatan. Topik yang diangkat meliputi hematologi, kimia klinik, imunoserologi, imunohematologi, sitohitoteknologi, mikrobiologi, parasitologi, dan toksikologi klinik.
Arjuna Subject : Profesi Kesehatan - Teknologi Laboratorium Medis
Articles 53 Documents
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MODIFIKASI METODE ISOLASI DNA CETYL TRIMETHYLAMMONIUM BROMIDE (CTAB) UNTUK SAMPEL EPITEL PIPI MANUSIA Maharani Pertiwi Koentjoro; Hidayah Sri Wilujeng; Astrina Dilla; Endry Nugroho Prasetyo
Journal of Indonesian Medical Laboratory and Science Vol 2 No 2: Oktober 2021
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v2i2.54

Abstract

Isolation of deoxyribonucleotide (DNA) is an important step in molecular analysis. In this process, DNA must be obtained in sufficient quantities and in good quality for any further analysis. The Cetyl Trimethylammonium Bromide (CTAB) method is commonly used in DNA isolation of plant or fungal. This method is an alternative in DNA isolation since it is easy and inexpensive. This study aims to modify the CTAB method for DNA isolation from human cheek epithelium for any molecular analysis. Epithelial cells were taken from the oral cavity of the researcher. The isolation protocol included cell lysis step with CTAB buffer and proteinase-K, purification step with the addition of chloroform:isoamylalcohol (24:1), precipitation step with isopropanol. The results of the ratio analysis of DNA spectrophotometer at wavelengths of 260 and 280 nm in the range of 1.73-1.85. The quality of DNA isolation was observed by agarose gel electrophoresis and a firm band was obtained after Ethidium Bromide staining. The DNA concentration in both methods ranged from 400-480 mg/mL. The time required for both methods ranges from 2.5-3 hours. The modified CTAB method DNA isolation protocol produces DNA that has good quality and quantity for molecular analysis processes, such as Polymerase Chain Reaction (PCR).
EFEKTIVITAS VARIASI KONSENTRASI TEPUNG TALAS SEBAGAI MEDIA ALTERNATIF PERTUMBUHAN JAMUR Candida albicans Marchamtia Sarah Nur Awalia Fajari; Muhammad Taufiq Qurrohman
Journal of Indonesian Medical Laboratory and Science Vol 2 No 2: Oktober 2021
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v2i2.56

Abstract

Taro flour (Colocasia esculenta (L.) Schott) has 77%% carbohydrate. A high carbohydrate source has the potential to be used as an alternative media in supporting the examination of Candida albicans fungal culture. Candida albicans is one of the fungi causing candidiasis. Therefore, this study aims to see the effectiveness of the variations of taro flour concentrations as an alternative media to the growth of fungus Candida albicans. The type of the study was experimental with a post test only control group design. The subjects of the study were taro flour media with concentrations of 0,12%, 0,24%, and 0,36% tested on fungus Candida albicans, which was inoculated by spread plate with PDA (Potato Dextrose Agar) media used as control. The data collection was performed by calculating the colony of Candida albicans, which grew in each media. The mean colony of each media on PDA, taro flour 0,12%, 0,24%, 0,36% were 15.5 colonies, 18.8 colonies, 19.5 colonies, and 20.8 colonies, respectively. The effectiveness test showed that all taro flour concentrations have a percentage more than 100%. The variation of taro flour has the growth effect of fungus Candida albicans. Thus, the higher the concentration of taro flour media, the more colony the number of Candida albicans grew. The effectiveness test showed that all taro flour concentrations was classified as very effective.
APLIKASI METRIK SIGMA DALAM PEMANTAPAN MUTU INTERNAL PADA PEMERIKSAAN UREUM DISALAH SATU LABORATORIUM RUMAH SAKIT KABUPATEN PANGANDARAN Rizal Apriansyah Pratama; Dewi Kania Yulianti; Doni Setiawan
Journal of Indonesian Medical Laboratory and Science Vol 2 No 2: Oktober 2021
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v2i2.64

Abstract

Intralaboratory Quality Control in clinical laboratories is significant and helps detect errors early to improve quality and guarantee examination results. The guaranteed method can be done by applying the sigma metric, which can detect 690,000 errors per million opportunities or 30.9 probability without defects with a minimum sigma value category of 3 and a maximum sigma value of 6. Urea is one of the clinical chemistry examination parameters that often has a sigma value <3. This study aims to determine the application of the sigma metric in internal quality assurance on urea examination in one of the Pangandaran district hospital laboratories. This research method is a descriptive-analytic based on the sigma value in internal quality assurance on urea examination in Pangandaran district hospital laboratories. Based on the study results, the value of sigma 3 - < 4 or marginal in March and May 2021 at the average control level while at the pathological control level increased from 2 sigma in March to 3 sigma in May 2021 said to be marginal.
PENGGUNAAN SIX SIGMA PADA PEMERIKSAAN JUMLAH LEUKOSIT DI RSUD PANEMBAHAN SENOPATI BANTUL Hieronymus Rayi Prasetya; Nurlaili Farida Muhajir; Magdalena Putri Iriyanti Dumatubun
Journal of Indonesian Medical Laboratory and Science Vol 2 No 2: Oktober 2021
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v2i2.72

Abstract

Internal quality assurance is a prevention and control activity that must be carried out by the laboratory continuously and covers all aspects of laboratory examination parameters. Hematology examination in the laboratory is carried out using a Hematology analyzer, but this tool has limitations, one of which is that it can make leukocyte count reading errors. In order for the results of the tool to be reliable, it is necessary to carry out quality control on the hematology analyzer. The use of Westgard multirule is commonly used in laboratories, but the application of six sigma is still very rarely used, especially in the field of hematology. This research aims to know the internal quality control of the analytical stage of the Hematology analyzer for the leukocyte count based on the analysis of Westgard and Six sigma. This type of research is descriptive research. The sample in this study is the control value data for the examination of the leukocyte count for 1 month at Panembahan Senopati Hospital. The data were analyzed using the Westgard rules and Six sigma. At low level, 13s deviation (random error) is obtained. At the normal level, there is a deviation of 12s (warning). At high level 12s deviation is obtained (warning). The sigma scale at all control levels shows a scale above 6. Analysis based on six sigma for leukocyte count showed an average of 7.16 sigma which indicates that leukocyte examination using a hematology analyzer has an accuracy of 99.9%.
PERBANDINGAN HASIL PEMERIKSAAN KONTAMINASI BAKTERI PADA PRODUK TROMBOCYTE CONCENTRATE METODE KONVENSIONAL DAN APHERESIS Resti Ariani; Ria Aidina
Journal of Indonesian Medical Laboratory and Science Vol 3 No 1: April 2022
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v3i1.47

Abstract

Platelet Concentrate (PC) is one of the blood products that is often used for transfusion. The storage temperature for TC products is in the range of 20-24OC which is the optimal temperature for bacterial growth. TC products contaminated with bacteria can cause a dangerous septic reaction. With the development of blood transfusion, apheresis methods emerged for the manufacture and aseptic collection of TC products. The apheresis method is considered safer than the conventional method which has a high chance of bacterial contamination. This study aims to compare the results of the examination of bacterial contamination in conventional TC products and apheresis methods. This study is a comparative descriptive study with a retrospective approach in the form of examining data on bacterial contamination of TC PMI Semarang City during 2019. The results of the examination of the TC apheresis method product showed that there were no TC products that were contaminated with bacteria (0.0%; 0 of 48), while in conventional method, there is 1 TC product that has bacterial contamination (0.3%; 1 out of 360). Fisher exact test on each TC product obtained p value = 1.00 (> 0.05) so that the difference that appears is not significant. Examination of bacterial contamination in conventional TC products and apheresis gave the same good results.
EKTRAK KULIT MANGGIS (Garcinia Mangostana L) SEBAGAI ALTERNATIF PENGGANTI EOSIN UNTUK PEMERIKSAAN TELUR CACING Apriani Apriani; Ereskadi Ereskadi
Journal of Indonesian Medical Laboratory and Science Vol 3 No 1: April 2022
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v3i1.62

Abstract

Direct examination of worm eggs was found to be colorless, making it difficult to see under a microscope. The use of Eosin 2% is a simple method of examining worm eggs commonly used in medical diagnosis and research. Some of the drawbacks of Eosin are high price and its ingredients can damage the environment. Another dye to consider is the mangosteen peel. Mangosteen peel contains anthocyanins which produce a red color. This study aims to see whether mangosteen peel (Garcinia mangostana L) can be used as an alternative for coloring intestinal nematode worm eggs. This study used a descriptive experimental method, namely observation by looking at the contrast, color absorption, and clarity of the egg in the microscope. The concentration used in this study were 1:1; 1:2; 1:3; 1:4 and 1:5. Staining using mangosteen peel (Garcinia mangostana L) at a concentration (1: 2) showed the quality of the staining was close to the quality of the 2% eosin staining (control). This makes mangosteen peel extract (Garcinia mangostana L) can be used as an alternative to eosin in examining worm eggs.
UJI ANTIFERTILITAS EKSTRAK LIDAH BUAYA (Aloe Vera L.) TERHADAP KUANTITAS SPERMATOZOA DAN EKSPRESI CYCLOOXYGENASE-2 PADA TESTIS MENCIT (Mus musculusL.) Rica Vera; Dedy Sambahtra
Journal of Indonesian Medical Laboratory and Science Vol 3 No 1: April 2022
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v3i1.65

Abstract

The family planning program needs to be carried out as an effort to reduce the rate of population growth. In this case the participation of men is very important and need research about natural ingredients that can be used as antifertility drugs. Aloe vera leaf (Aloe vera L.) is one of the plants that have an antifertility effect so that it can be used as a contraceptive for men. The purpose of this study was to determine the effect of ethanol extract  aloe vera leaves of the quantity of spermatozoa and the expression of Cyclooxygenase-2 (COX-2) in the testes of mice (Mus musculus L.). The research used four treatment groups, namely P0 as a control was given aquadest 0,5 mL, P1 was given extract 15 mg/25g body weight by oral, P2 was given extract 20 mg/25g body weight by oral, and P3 was given extract 25 mg/25g body weight by oral given for 4 weeks. The research used completely randomized sampling method and data were analyzed using One Way ANOVA followed by Bonferroni Post Hoc test 5%. Administration of ethanol extract aloe vera leaves resulted in a decreased sperm quantity and increase expression of COX-2 in the testes of mice so that aloe vera leaves had the opportunity as a herbal contraceptive for men.
PENGARUH VARIASI KONSENTRASI SARI PATI BUAH SUKUN SEBAGAI ALTERNATIF MEDIA SEMI SINTETIK PADA PERTUMBUHAN JAMUR Candida albicans Reza Yuliana; M. Taufiq Qurrohman
Journal of Indonesian Medical Laboratory and Science Vol 3 No 1: April 2022
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v3i1.67

Abstract

Gold standard examination Candida sp.  is a culture test.  The abundance natural resources in Indonesia encourages researchers to take advantage the wealth of natural resources to create alternative semi-synthetic media for mushroom growth from breadfruit.  The purpose of this study was determining the effective concentration of alternative semi-synthetic media of breadfruit essence as growth medium for the fungus Candida albicans. This research is experimental study. Observations were made macroscopically and confirmed microscopically. The results showed that average growth of the colonies on PDA media was 15.50, for the alternative breadfruit media the average of colonies was 24.50 (100% concentration), 19.75 (90% concentration), 19.50 (80% concentration), 16.00 (70% concentration), 15.50 (60% concentration). The results of the ANOVA test obtained value <0.05, so H1 was accepted there was a significant difference in the average growth of fungi in the media of breadfruit essence. The conclusion from this research is that the concentration of 60% breadfruit essence is the effective for growing Candida albicans.
HUBUNGAN KADAR KARBOKSIHEMOGLOBIN DENGAN HEMATOKRIT DALAM DARAH MAHASISWA TINGKAT AKHIR TERPAPAR KARBONMONOKSIDA DI SEKOLAH TINGGI ILMU KESEHATAN NASIONAL Wijanarko Wijanarko; Mastuti Widi Lestari
Journal of Indonesian Medical Laboratory and Science Vol 3 No 1: April 2022
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v3i1.69

Abstract

Carboxyhemoglobin is a substance formed due to the bonding of carbon monoxide with haemoglobin, which results in a lack of oxygen in the body, causing polycythemia which leads to an increase in blood hematocrit. motorized. This research method uses quantitative. The subjects of this study amounted to 30 people where the subjects were divided into 2 categories, namely smokers and motorcyclists. Data analysis using Shapiro Wilk and Rank Spearman Based on the research, it was found that the average COHb level was 6.06 while the average hematocrit in the normal range obtained p values ??> 0.05 for COHb 0.827, 0.836 and 0.827 while the hematocrit was 0.827, 0.836 and 0.827. This study concludes that there is no relationship between carboxyhemoglobin levels and hematocrit levels in final year students who are exposed to carbon monoxide from cigarette smoke and motor vehicle fumes.
UJI EFEKTIFITAS EKSTRAK DAUN PANDAN WANGI (Pandanus amaryllifolius Roxb) TERHADAP PERTUMBUHAN BAKTERI Streptococcus mutans Siti Juariah; Junia Wiranda; Harni Sepryani
Journal of Indonesian Medical Laboratory and Science Vol 3 No 1: April 2022
Publisher : Asosiasi Institusi Pendidikan Tinggi Teknologi Laboratorium Medik Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53699/joimedlabs.v3i1.75

Abstract

Fragrant pandan leaves (Pandanus amaryllifolius Roxb) is a type of plant that contains flavonoid compounds, alkaloids, saponins and tannins that can function as antibacterial. Streptococcus mutans is one of the bacteria that causes dental caries. Fragrant pandan leaf extract is expected to be an alternative to overcome bacterial infections such as dental caries. Dental caries is a disease caused by damage to the enamel layer that can extend to the nerves of the teeth so that it will cause cavities caused by the bacterium Streptococcus mutans. The aim of this study was to determine the effectiveness of fragrant pandan leaf extract against the growth of Streptococcus mutans bacteria that causes dental caries. This type of research is a laboratory experiment using the disc diffusion method. Fragrant pandan leaves were extracted by maceration method using 96% ethanol as solvent. The results of this study showed that the pandan fragrant leaf extract had an inhibitory effect on the growth of Streptococcus mutans bacteria at concentrations of 25%, 50%, and 75% with an average inhibition zone diameter of 10.3 mm, 13.3 mm, 17.6. mm. Positive control (amoxicilin) ??44.3 mm and negative control (aquadest) had no value to inhibit bacterial growth. The conclusion is that pandan leaves extract (Pandanus amaryllifolius Roxb) can inhibit Streptococcus mutans which is the bacteria that causes dental caries.

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