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Berkala Ilmiah Kimia Farmasi

BIKFAR
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Published by Universitas Airlangga

ISSN : 23028270 EISSN : 28081048 DOI : http://dx.doi.org/10.20473/bikfar.v8i2.31763

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Chemistry Medicine & Pharmacology

to facilitate interaction, discussion, and updating of research ideas in the fields of pharmacy, Pharmaceutical Chemistry, Medicinal Chemistry, Drug Development, Organic synthesis, Analytical chemistry, Pharmaceutical Technology, Pharmaceutical Microbiology and Biotechnology, Pharmacology and Toxicology, Nutraceutical / functional foods.

Arjuna Subject : Pharmacology, Toxicology and Pharmaceutics - Farmakologi

Articles 30 Documents

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Antioxidant and inhibitory activity of Roselle Extract (Hibiscus sabdariffa L.) against Methicillin-Resistant Staphylococcus aureus (MRSA) Nuril Fikriyah; Isnaeni Isnaeni; Asri Darmawati
Berkala Ilmiah Kimia Farmasi Vol. 8 No. 1 (2021): June
Publisher : Universitas Airlangga

Background: Hibiscus sabdariffa known as roselle, which belongs to the family Malvaceae, grows in sub-tropical and tropical region including Indonesia. Several studies have reported the benefits of roselle for health. Roselle extract has been shown to have antibacterial effect as a support for antibacterial therapy, especially for case of antibiotic resistance, and antioxidant effect which can neutralize free radicals. Purpose: The aim of this study was obtaining the minimum inhibitory concentration (MIC) and inhibition concentration (IC50) of roselle extract. Methods: Antibacterial effect of roselle extract against Methicillin-Resistant Staphylococcus aureus (MRSA) was tested in nutrient agar media using diffusion method. Antioxidant activity of roselle extract was performed by DPPH. The violet color of DPPH solutions that was reduced by roselle extract were measured using visible spectrophotometer at the wavelength of 516 nm. Result: The result of this study obtained the (MIC) and IC50 of roselle extract were 2,5% and 1251±202,32 ppm, respectively. Conclusion: It can be concluded that roselle extract have antibacterial effect against MRSA and antioxidant effect.

Analgesic Activity of Acyl-Salicylic Acid Derivatives And In Silico Docking Study For Their Potency As Cyclooxygenase-2 Inhibitors Nuzul Wahyuning Diyah; Anindi Lupita Nasyanska; Bambang Tri Purwanto; Siswandono Siswandono
Berkala Ilmiah Kimia Farmasi Vol. 7 No. 2 (2020): DESEMBER
Publisher : Universitas Airlangga

A series of acyl salicylic acid derivatives were screened to investigate their analgesic activities and their potency as cyclooxygenase-2 (COX-2) inhibitors. Fourteen compounds (BS1–14) were assayed by acetic acid induced writhing test. Their ability for interaction with COX-2 was studied through a docking simulation at the COX-2 active site (PDB. 5IKQ). The results of the analgesic activity test gave 3 compounds that produce ED50< 0.39 mmol/kg body weight, lower than aspirin as a positive control. The compounds BS3 and BS4 showed excellent analgesic activity and the tert-butyl substituted molecule BS3 (O-(4-tert-butylbenzoyl)-salicylic acid analog) showed the highest analgesic activity with ED50 of 0.26 mmol/kg. Based on in silico molecular docking, it is known that almost all of the tested ligands (12 compounds) showed a higher binding affinity for COX-2 than meclofenamic acid which is a COX-2 inhibitory NSAID. The results of in vivo analgesic activity were justified with the outcome of in silico investigation. Molecular docking of acyl-salicylates confirmed in vivo experiments and it was found that BS3 was the most active compound as an analgesic agent and the most potent as a COX-2 inhibitor among the evaluated compounds.a

Solid Soap Formulation with Ethanol Extract of Ginger Rhizome (Zingiber officinale Rosc.) Combination of Virgin Coconut Oil (Vco) and Palm Oil Risma Andriani; Yani Ambari; Iif Hanifa Nurrosyidah
Berkala Ilmiah Kimia Farmasi Vol. 8 No. 2 (2021): December
Publisher : Universitas Airlangga

Soap is a type of cleaning product that is used to clean dust, oil, dirt, germs, and bacteria that stick to the skin. Ginger is a plant that contains a wide range of antibacterial compounds including E. Coli, Staphylococcus aureus, B. Cereus, L monocytogenes and Candida albicans bacteria. Soap is made by chemically reacting sodium or potassium compounds with fatty acids derived from vegetable oil or animal oil. This study aims to determine the physical characteristics of solid soap with ginger rhizome extract with a combination of coconut oil and palm oil and analyze the panelist’s response to the soap produced. In the third week of observation, it is known that the water content ranges from 0,07%-0,1% with foam stability 71,11%-88,23% and pH 8,96-9,08. The most preferred solid soap based on the response of the panelist is soap from a mixture of coconut oil and palm oil with a ratio of 15:5.

Produksi Enzim Fibrinolitik Tempe oleh Rhizopus oryzae FNCC 6078 Alicia Sada; Noor Erma Sugianto; Achmad Toto Poernomo
Berkala Ilmiah Kimia Farmasi Vol. 8 No. 1 (2021): June
Publisher : Universitas Airlangga

ackground Rhizopus oryzae FNCC 6078 had been evaluated producing fibrinolytic enzyme under solid state fermentation. Soybean had been used to produce fibrinolytic enzyme through fermentation in tempeh. The main purpose of this study was to reveal optimum condition for fermentation. The parameters of the condition were inoculum volume, incubation period and temperature. Optimum condition was defined by maximum fibrinolytic activity. Methode Fibrinolytic activity was measured using spectrophotometry at 274 nm. Result optimum condition for producing fibrinolytic enzyme was 1,5 mL volume of inoculum of Rhizopus oryzae suspension in 25%T, 42 hours for incubation period and 35oC temperature incubation.

Inhibitory Activity of Fermented Milk Multi Strain Lactic Acid Bacteria Against Staphylococcus Aureus ATCC 25923 Ambarini Indah; Noor Erma Nasution; Achmad Toto Poernomo; Isnaesi Isnaeni
Berkala Ilmiah Kimia Farmasi Vol. 7 No. 1 (2020): JUNI
Publisher : Universitas Airlangga

The aim of the study was to investigate inhibitory activity of fermented milk multi strain lactic acid bacteria (LAB): Lactobacillus casei Shirota, Lactobacillus acidophilus, and Lactobacillus bulgaricus against Staphylococcus aureus ATCC 29523 at variable concentration milk using diffusion method. The multi strain culture of LAB were inoculated on skimmed and diary cow milk (DCM) at concentration of 50% and 100%; in which carbon, amino acid, fat and water concentration were different. Effect of the milk and its concentration on the inhibitory activity against the test bacteria was observed as well as decreasing milk acidity and increasing of milk viscosity after 24 hours incubation. Results showed that the performance of LAB in two kinds of milks at 50% and 100% concentrations were different. On the lower milk concentration, the acidity was lower than on the 100% milk, while on the higher milk concentration, the viscosity was bigger than on the 50% concentration. The fermented milk of L. cassei Sirota did not show inhibitory activity. The multi strain of LAB on the DCM at concentration of 50% and 100% showed antibacterial activity againts Staphylococcus aureus ATCC 25923, while the skimmedmed milk fermentation showed lower inhibitory activity on both concentrations.

Qualitative Analysis of Rhodamine B in Shrimp Paste at Sumbawa Besar City Markets Using Thin Layer Chromatography Abby Rahmat Kamaruzzaman; Asri Darmawati; Djoko Agus Purwanto
Berkala Ilmiah Kimia Farmasi Vol. 8 No. 2 (2021): December
Publisher : Universitas Airlangga

Terasi biasa diberi pewarna merah agar lebih menarik. Tahun 2013, pernah ditemukan Rhodamin B dalam produk terasi yang beredar di pasar kabupaten Sumbawa Barat. Rhodamin B adalah pewarna merah yang dilarang digunakan untuk makanan. Diantara berbagai produk terasi yang dijual di Sumbawa, terasi khas asli Sumbawa yaitu terasi Empang, perlu dijaga keamanannya agar dapat lebih dipromosikan. Tujuan penelitian ini adalah membuktikan bahwa terasi yang dijual pasar kota Sumbawa Besar tidak mengandung Rhodamin B. Metode yang digunakan untuk identifikasi Rhodamin B adalah kromatografi lapis tipis (KLT). Penelitian ini menggunakan silika gel 60 F254 sebagai fase diam dan fase gerak terpilih yaitu N-butanol:etil asetat:amonia 25% (10:4:5). Sampel adalah produk terasi yang dijual di Pasar Kota Sumbawa Besar, periode sampling bulan Februari dan Maret 2021. Preparasi sampel terasi dengan cara diekstraksi menggunakan etanol dan volume ekstrak yang ditotolkan pada lempeng KLT adalah 2 μl. Parameter validasi metode yang diuji adalah spesifisitas/selektifitas dan batas deteksi (LOD). Hasil penelitian menunjukkan metode ini spesifik untuk Rhodamine B yaitu Rf 0,69, profil spektra yang sama antara baku dan sampel yang diadisi Rhodamin B. Resolusi (Rs) antara noda Rhodamin B dengan noda terdekat lain dalam sampel adalah >1,5. Panjang gelombang serapan maksimum Rhodamin B adalah 544 nm. Nilai LOD sebesar 4,14 ng. Hasil identifikasi terhadap 10 sampel yang terdiri dari 3 sampel terdaftar di BPOM dan 7 sampel tidak terdaftar di BPOM tidak terdeteksi adanya Rhodamin B. Kesimpulan dari penelitian ini adalah semua sampel terasi yang dijual di pasar kota Sumbawa Besar tidak terdeteksi mengandung Rhodamin B.

Validasi Metode KLT-Densitometri Pada Penetapan Kadar (-)-Epigallocatechin Gallate (EGCG) Dalam Teh Hijau Chusnul Chatimah; Sugijanto sugianto; Achmad Toto Poernomo
Berkala Ilmiah Kimia Farmasi Vol. 7 No. 2 (2020): DESEMBER
Publisher : Universitas Airlangga

Latar Belakang Daun teh (Camellia sinensis L.) memiliki banyak manfaat untuk kesehatan. Daun teh hijau mengandung banyak senyawa polifenol dalam kadar tinggi, antara lain turunan catechin epicatechin (EC), epigallocatechin (EGC), epicatechin gallate (ECG) dan epigallocatechin gallate (EGCG). Komponen bioaktif yang paling dominan adalah EGCG. Sebuah metode TLC-Densitometry sederhana telah dikembangkan untuk penentuan (-)-Epigallocatechin gallate dalam produk teh hijau. Metode ini menggunakan silika gel F 254 sebagai fase diam dan fase gerak kloroform-asam asetat-asam format-isopropanol (16:2:2:8). Hasil Panjang gelombang maksimum terdeteksi pada 278 nm. Respon linieritas ditemukan (-)-Epigallocatechin gallate antara 0,5043 g–2,5215 g dengan regresi persamaan Y = 5409,8 X – 569,97 (r = 0,9996; Vxo= 3,94%). Metode divalidasi untuk menentukan deteksi tiruan (0,0188 g), kuantisasi batas (0,0627µg), akurasi (91,41%), presisi untuk standar EGCG (4,47%) dan presisi metode (7,01 %). Kesimpulan hasil ini menunjukkan bahwa EGCG dalam produk teh hijau dapat dideteksi dengan metode TLC-Densitometry. Dengan menggunakan metode, konsentrasi EGCG pada produk teh hijau adalah 3,33%.

Screening and Identification of Fibrinolitic Bacteria from Tempeh Muhammad Hakim Rafiga; Achmad Toto Poernomo; Sudjarwo Sudjarwo
Berkala Ilmiah Kimia Farmasi Vol. 7 No. 1 (2020): JUNI
Publisher : Universitas Airlangga

Tempe is a food that is well known by indonesian people. The purpose of this study was to obtain bacteria with the largest fibrinolytic activity from Tempe. This research sample was obtained from four different markets in Surabaya area. Proteolytic activity of bacteria is examined using Skim Milk Agar media. Followed by fibrinolytic examination on the fibrin plate media. Identification of bacteria is carried out by gram staining method and 16S rRNA method. The sample is prepared by mixing the sample with a normal saline solution until 10-7 dilution is obtained. Then the bacterial suspension is spread on SMA media then incubated at 37°C for 24 hours. The bacterial suspension is spread onSMA media then incubated at 37°C for 24 hours. The selection of fibrinolytic protease producing bacteria was carried out using Skim Milk Agar (SMA) and Fibrin Agar (FA) are characterized by the presence of clear zones around them. Bacteria that produce fibrinolytic activity are then cultured in the Nutrrient Agar medium. The bacteria was then tested for fibrinolytic activity on the Fibrin Plate media in incubation at 37°C for 24 hours. Positive bacteria are calculated by measuring the diameter of clear zones. Bacteria with sample code T2.2 produce the largest fibrinolytic index. T2.2 bacteria are then microscopically characterized, macroscopically, and bacterial isolates showing fibrinolytic activities were identified using Polymerase Chain Reaction (PCR) targeting their 16S rRNA gene. T2.2 bacteria is thought to be Stenotrophomonas maltophilia with a percentage similarity 96%.

Anti-bacterial activity of Rosela Flower Extract (Hibiscus sabdariffa L.) against Extended-Spectrum Beta-Lactamase (ESBL) Eschericia coli Muhammad Mu’amar Fathoni; Isnaeni Isnaeni; Asri Darmawati
Berkala Ilmiah Kimia Farmasi Vol. 8 No. 1 (2021): June
Publisher : Universitas Airlangga

Roselle (Hibiscus sabdariffa L.) contains cyanidin-3-rutinoside, delphinidin, delphinidin-3-monoglucoside, cyanidin-3-monoglucoside, cyanidin-3-sambubioside, cyanidin-3,5-diglucoside may inhibit the growth of Extended-Spectrum-Beta-Lactamase (ESBL) Escherichia coli, but there is no research reported the determination of MIC of Roselle (Hibiscus sabdariffa L.) extract powder against ESBL E.coli ATCC 6110 and ATCC 5949 and their potency ratio compared to meropenem. This study aimed to determine the MIC of Roselle flower extract powder on the growth of ESBL E. coli ATCC 6110 and ATCC 5949 and determine the potency ratio compared to meropenem. This study used two methods in determining MIC, namely the agar diffusion method and the dilution method with Nutrient Agar media which was incubated at 37 ±1°C for 24 hours, while the determination of the potency ratio was carried out by diffusion method with the same media which was incubated at 37 ±1°C for 24 hours. The results obtained were diameter of inhibition zone (mm) which were then observed and analyzed to calculate the potency ratio. The results showed that the MIC of Roselle flower extract powder was obtained at a concentration of 12,500 ppm by diffusion method and at a concentration of 3,125 ppm by dilution method with 24 hours incubation at 37 ± 1o C and the potency ratio of Roselle flower extract powder compared to meropenem was 89.7% and 97.97% against ESBL E.coli ATCC 6110 and ESBL E.coli ATCC 5949 respectively.

Pengaruh Sumber Karbon Organik Terhadap Produksi Protease Fibrinolitik Bacillus Sphaericus BM 9.1 Dengan Solid State Fermentation (SSF) Afrilia Diana Fitri; achmad Toto Poernomo; Muhammad Faris Adrianto
Berkala Ilmiah Kimia Farmasi Vol. 7 No. 2 (2020): DESEMBER
Publisher : Universitas Airlangga

Enzim fibrinolitik merupakan kelompok enzim protease serin yang mampu menghancurkan bekuan darah (fibrin) pada berbagai penyakit trombotik. Sumber enzim fibrinolitik dapat diperoleh dari mikroorganisme. Bacillus sp merupakan salah satu mikroorganisme yang berpotensi menghasilkan enzim fibrinolitik. Medium memiliki peran penting dalam menumbuhkan dan memproduksi enzim fibrinolitik. Dalam medium, sumber karbon yang cukup diperlukan untuk pertumbuhan bakteri. Dalam fermentasi mikroba, sumber karbon sangat diperlukan untuk pembentukan biomassa dan produksi energi. Media yang digunakan dalam penelitian ini adalah media Nutrient agar yang ditambahkan sumber karbon organik. Sumber karbon yang digunakan adalah glukosa, fruktosa, sukrosa, dan pati dengan konsentrasi masing-masing 1% b/v. Penambahan sumber karbon dengan konsentrasi 1% b/v pada medium menunjukkan pengaruh yang berbeda terhadap produksi enzim fibrinolitik oleh Bacillus sphaericus BM 9.1. Jadi di antara semua sumber karbon yang diuji, fruktosa menghasilkan indeks fibrinolitik tertinggi (2,86 ± 0,03). Langkah selanjutnya adalah mengoptimalkan konsentrasi sumber karbon fruktosa. Optimasi konsentrasi fruktosa diuji pada konsentrasi 1%, 2%, 3%, dan 4%. Semua konsentrasi telah diuji, konsentrasi fruktosa 1% b/v menghasilkan indeks fibrinolitik tertinggi (2,82 ± 0,04).

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Jawa timur

INDONESIA

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Marcellino Rudyanto

Contact Email marcellino-r@ff.unair.ac.id

Phone +628165434137

Journal Mail Official bikfar@ff.unair.ac.id

Editorial Address Department Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Airlangga Surabaya, Kampus C, Mulyorejo, Surabaya-60115, East Java, Indonesia

Location Kota surabaya, Jawa timur INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Marcellino Rudyanto

Contact Email
marcellino-r@ff.unair.ac.id

Phone
+628165434137

Journal Mail Official
bikfar@ff.unair.ac.id

Editorial Address
Department Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Airlangga Surabaya, Kampus C, Mulyorejo, Surabaya-60115, East Java, Indonesia

Location
Kota surabaya,

Jawa timur

INDONESIA