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Contact Name
Marcellino Rudyanto
Contact Email
marcellino-r@ff.unair.ac.id
Phone
+628165434137
Journal Mail Official
bikfar@ff.unair.ac.id
Editorial Address
Department Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Airlangga Surabaya, Kampus C, Mulyorejo, Surabaya-60115, East Java, Indonesia
Location
Kota surabaya,
Jawa timur
INDONESIA
Berkala Ilmiah Kimia Farmasi
Published by Universitas Airlangga
Core Subject : Health, Science,
to facilitate interaction, discussion, and updating of research ideas in the fields of pharmacy, Pharmaceutical Chemistry, Medicinal Chemistry, Drug Development, Organic synthesis, Analytical chemistry, Pharmaceutical Technology, Pharmaceutical Microbiology and Biotechnology, Pharmacology and Toxicology, Nutraceutical / functional foods.
Articles 20 Documents
Screening and Identification of Fibrinolitic Bacteria from Tempeh Muhammad Hakim Rafiga; Achmad Toto Poernomo; Sudjarwo Sudjarwo
Berkala Ilmiah Kimia Farmasi Vol. 7 No. 1 (2020): JUNI
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (334.965 KB) | DOI: 10.20473/bikfar.v7i1.29057

Abstract

Tempe is a food that is well known by indonesian people. The purpose of this study was to obtain bacteria with the largest fibrinolytic activity from Tempe. This research sample was obtained from four different markets in Surabaya area. Proteolytic activity of bacteria is examined using Skim Milk Agar media. Followed by fibrinolytic examination on the fibrin plate media. Identification of bacteria is carried out by gram staining method and 16S rRNA method. The sample is prepared by mixing the sample with a normal saline solution until 10-7 dilution is obtained. Then the bacterial suspension is spread on SMA media then incubated at 37°C for 24 hours. The bacterial suspension is spread onSMA media then incubated at 37°C for 24 hours. The selection of fibrinolytic protease producing bacteria was carried out using Skim Milk Agar (SMA) and Fibrin Agar (FA) are characterized by the presence of clear zones around them. Bacteria that produce fibrinolytic activity are then cultured in the Nutrrient Agar medium. The bacteria was then tested for fibrinolytic activity on the Fibrin Plate media in incubation at 37°C for 24 hours.  Positive bacteria are calculated by measuring the diameter of clear zones. Bacteria with sample code T2.2 produce the largest fibrinolytic index. T2.2 bacteria are then microscopically characterized, macroscopically, and bacterial isolates showing fibrinolytic activities were identified using Polymerase Chain Reaction (PCR) targeting their 16S rRNA gene. T2.2 bacteria is thought to be Stenotrophomonas maltophilia with a percentage similarity 96%.
Validasi Metode Spektrofotometri UV Untuk Penetapan Kadar Kolkisin Dalam Infus Kembang Sungsang Muhammad Muslich; Isnaeni Isnaeni; Sudjarwo Sudjarwo
Berkala Ilmiah Kimia Farmasi Vol. 7 No. 1 (2020): JUNI
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (306.588 KB) | DOI: 10.20473/bikfar.v7i1.29058

Abstract

Validation of colchicine analysis methods in herbal infusions has been carried out to facilitate quality control of raw materials or simplicia. The validation parameters of the method set include selectivity, linearity, precision, and accuracy, which are included in category I. In this study, the raw material was prepared from the leaves of Gloriosa superba Linn. infusion made. The results of the selectivity test with colchicine as a standard provide uptake at a chosen wavelength of 340.2 nm. Linearity test results show the equation y = 0.0303x + 0.0131 with r = 0.9998> r Table for n = 6, ie 0.917 for p <0.01 and 0.811 for p = <0.05, the value of Vxo is 1 15%. In the precision test, the coefficient of variation is 0.54% and the accuracy test gives an average recovery of 92.81% ± 4.16%. Determination of colchicine in the infusion preparation was carried out as many as three replications. The results showed that the content of colchicine in raw materials was 0.3152% ± 1.99%, while the colchicine content in herbal infusion preparations was 0.483 ± 4.47%.
Inhibitory Activity of Probiotic Milk Against Eschericia coli ATCC 6538 and Staphylococcus aureus ATCC 8739 Andyanita Hanif Hermawati; Aryati Aryati; Isnaeni Isnaeni
Berkala Ilmiah Kimia Farmasi Vol. 7 No. 1 (2020): JUNI
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (549.674 KB) | DOI: 10.20473/bikfar.v7i1.29059

Abstract

The aim of this research is to evaluate inhibitory activity of Lactobacillus acidophilus (L.a) and Lactobacillus casei (L.c) probiotic milk against Escherichia coli and Staphylococcus aureus growth. The fermented milk was prepared by inoculating each probiotic in milk at 43ºC and then incubated at room temperature for 24 hours. Diffusion agar method by using nutrient agar media was used to assess the minimum inhibition concentration (MIC). Obtained results showed pH of fermentation probiotic milk 5 + 0,05. Optimum inhibition zone diameter of L. acidophilus fermented milk against Escherichia coli and Staphylococcus aureus at MIC value of 14 mm. Inhibition zone diameter of L. casei fermented milk obtained an optimum against Escherichia coli and Staphylococcus aureus at MIC value of 12 mm and 13 mm respectively. The L. acidophilus and Lactobacillus casei probiotics fermented milk exhibited considerable antibacterial agent against Escherichia coli and Staphylococcus aureus.
Optimasi Produksi Fibrinolitik oleh Bacillus megaterium BM 9.1 menggunakan media Limbah Pertanian sebagai sumber karbon Kiki Lestari; Achmad Toto Poernomo; Prawita A
Berkala Ilmiah Kimia Farmasi Vol. 7 No. 1 (2020): JUNI
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bikfar.v7i1.29060

Abstract

The study of effect agro recidues carbon sources on fibrinolytic enzyme production by Bacillus megaterium BM 9.1 was done by using fibrin plate method. Bacillus megaterium BM 9.1 grown on agro residues medium with solid state fermentation. The fibrinolytic enzymes activity is declared by fibrinolytic index. Fibrinolytics index is the comparisson between clear zones and wall diameters. From various carbon sources tested, Bacillus megaterium BM 9.1 which is grown in banana peel, pineaple skin, corn husk, rice bran and corn cob could produce fibrinolytic enzymes. Optimum fibrinolytic activities found in a nutrient agar  and banana peel medium at fibrinolytic index 4,95 ± 0,08 (p<0,05). The comparisson fibrinolytic index  between Bacillus megaterium BM 9.1 which grown in combination nutrient agar, banana peel and nutrien agar standart is 117,%. Banana peel concentration optimation was done at concentration of 0,5 %, 1 %, 2 % and 4 % and the fibrinolytic index was found respectively according to 4,26 ± 0,11 ; 4,32 ± 0,03 ; 4,61 ± 0,03; and 4,35 ± 0,05. The optimum fibrinolytic activity was found in banana peel on consentration 2 %.
Inhibitory Activity of Fermented Milk Multi Strain Lactic Acid Bacteria Against Staphylococcus Aureus ATCC 25923 Ambarini Indah; Noor Erma Nasution; Achmad Toto Poernomo; Isnaesi Isnaeni
Berkala Ilmiah Kimia Farmasi Vol. 7 No. 1 (2020): JUNI
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (280.59 KB) | DOI: 10.20473/bikfar.v7i1.29064

Abstract

The aim of the study was to investigate inhibitory activity of fermented milk multi strain lactic acid bacteria (LAB): Lactobacillus casei Shirota, Lactobacillus acidophilus, and Lactobacillus bulgaricus against Staphylococcus aureus ATCC 29523 at variable concentration milk using diffusion method. The multi strain culture of LAB were inoculated on skimmed and diary cow milk (DCM) at concentration of 50% and 100%; in which carbon, amino acid, fat and water concentration were different. Effect of the milk and its concentration on the inhibitory activity against the test bacteria was observed as well as decreasing milk acidity and increasing of milk viscosity after 24 hours incubation. Results showed that the performance of LAB in two kinds of milks at 50% and 100% concentrations were different. On the lower milk concentration, the acidity was lower than on the 100% milk, while on the higher milk concentration, the viscosity was bigger than on the 50% concentration. The fermented milk of L. cassei Sirota did not show inhibitory activity. The multi strain of LAB on the DCM at concentration of 50% and 100% showed antibacterial activity againts Staphylococcus aureus ATCC 25923, while the skimmedmed milk fermentation showed lower inhibitory activity on both concentrations.
Pengaruh Sumber Karbon Organik Terhadap Produksi Protease Fibrinolitik Bacillus Sphaericus BM 9.1 Dengan Solid State Fermentation (SSF) Afrilia Diana Fitri; achmad Toto Poernomo; Muhammad Faris Adrianto
Berkala Ilmiah Kimia Farmasi Vol. 7 No. 2 (2020): DESEMBER
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (567.602 KB) | DOI: 10.20473/bikfar.v7i2.29269

Abstract

Enzim fibrinolitik merupakan kelompok enzim protease serin yang mampu menghancurkan bekuan darah (fibrin) pada berbagai penyakit trombotik. Sumber enzim fibrinolitik dapat diperoleh dari mikroorganisme. Bacillus sp merupakan salah satu mikroorganisme yang berpotensi menghasilkan enzim fibrinolitik. Medium memiliki peran penting dalam menumbuhkan dan memproduksi enzim fibrinolitik. Dalam medium, sumber karbon yang cukup diperlukan untuk pertumbuhan bakteri. Dalam fermentasi mikroba, sumber karbon sangat diperlukan untuk pembentukan biomassa dan produksi energi. Media yang digunakan dalam penelitian ini adalah media Nutrient agar yang ditambahkan sumber karbon organik. Sumber karbon yang digunakan adalah glukosa, fruktosa, sukrosa, dan pati dengan konsentrasi masing-masing 1% b/v. Penambahan sumber karbon dengan konsentrasi 1% b/v pada medium menunjukkan pengaruh yang berbeda terhadap produksi enzim fibrinolitik oleh Bacillus sphaericus BM 9.1. Jadi di antara semua sumber karbon yang diuji, fruktosa menghasilkan indeks fibrinolitik tertinggi (2,86 ± 0,03). Langkah selanjutnya adalah mengoptimalkan konsentrasi sumber karbon fruktosa. Optimasi konsentrasi fruktosa diuji pada konsentrasi 1%, 2%, 3%, dan 4%. Semua konsentrasi telah diuji, konsentrasi fruktosa 1% b/v menghasilkan indeks fibrinolitik tertinggi (2,82 ± 0,04).  
The Effect of Methyl and Chloro Substituent Compounds in Amida Derivatives Synthesis from p-Metoxicynamic Acid with Microwaves Irradiation Ruri Intan Nurcahyaningtyas; Juni Ekowati Ekowati; Marcellino Rudyanto
Berkala Ilmiah Kimia Farmasi Vol. 7 No. 2 (2020): DESEMBER
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (304.52 KB) | DOI: 10.20473/bikfar.v7i2.29300

Abstract

Background: The difference in the nature of these aromatic amine substituents, i.e. methyl and chloro will affect the N atom of aniline as a nucleophile to attack the carbonyl C atom in the p-methoxcycinnamoyl chloride in the synthesis two amides derivate of p-methoxycinnamic acid, namely N-(p-methylphenyl)-p-methoxycinnamide and N-(p-chlorophenyl)-p-methoxycinnamide. Aim: to obtain the N-(p-methylphenyl)-p-methoxycinnamide and the N-(p-chlorophenyl)-p-metoxicinamide compound from p-methoxycinamic acid using the microwave irradiation method as source of energy. Beside that, it also to determine the effect of the presence of methyl and chloro substituents in para position of aromatic amines in the yields of reactions. Method: The reactions were carried out by microwave irradiation at three powers, i.e 120 watts, 200 watts, 280 watts. After separation and purification steps, the products were identified by spectrometric methods. Result: At power of 200 watts for reaction time of 7.5 minutes, the yield of N-(p-methylphenyl)-p-methoxycinamide is larger than N-(p-chlorophenyl)-p-methoxycinnamamide. The percentage of the product synthesis of N-(p-methylphenyl)-p-methoxycinamide was 51.84% and the percentage of N-(p-chlorophenyl)-p-methoxycinnamamide was to 47.20%. Conclusion: The effect of substituent methyl is increase the percentage yield of N-(p-methylphenyl)-p-methoxycinamide compound than that substituent chloro of N-(p-chlorophenyl)-p-methoxycinamide compound under the same reaction conditions. Based on the identification of the structure of the synthesized compound using a UV spectrophotometer, infrared spectrophotomers and 1H-NMR spectrometer it can be concluded that the synthesized compounds are N-(p-methylphenyl)-p-methoxycinnamide and N- (p-chlorophenyl)-p-methoxycinnamide.  
Inhibitory Activity Of Roselle Flower (Hibiscus Sabdariffa L.) Aqueous Extract - Sodium Alginate Gel Against Staphylococcus aureus ATCC 25923 Esti Hendradi; Isnaeni Isnaeni; Arya Putra Damari
Berkala Ilmiah Kimia Farmasi Vol. 7 No. 2 (2020): DESEMBER
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (327.334 KB) | DOI: 10.20473/bikfar.v7i2.29301

Abstract

Staphylococcus aureus is one type of bacterium that causes skin infections resistant to some antibiotics. This encourages research to find new antibacterials by using substances from natural sources, often called medicinal plants. Aqueous extract of Roselle (Hibiscus sabdariffa L.) flower (AERF) was selected as one of natural ingredients for antibacterial in gel dosage form, in which sodium alginate was used as a matrix base. In this study, determination of the sodium alginate concentration effects (2.5%, 3% and 3.5%) on the properties of the gel was performed. The characteristics evaluated included pH, viscosity, and dispersive power; that should be met standard requirement and the gel ability to inhibit Staphylococcus aureus ATCC 25923 growths.  A diffusion test method was used to determine the Minimum Inhibitory Concentration (MIC) of the AERF powder against the test bacteria. The study results showed that AERF gel preparation with a composition of 3% sodium alginate and 3% roselle extract had better properties (pH, viscosity, and dispersive power) than the matrix and extract concentrations of 2.5% and 3.5% respectively. It was found that the optimum formula obtained a MIC of 0.04 mg/ml. In line with these results, it is expected that the AERF gel preparation will be stable, safe, and acceptable when used and might be recommended as antibacterial topical dosage form against Staphylococcus aureus. In the future, inhibitory activity against resistant pathogenic bacteria could be evaluated and developed.  
Analgesic Activity of Acyl-Salicylic Acid Derivatives And In Silico Docking Study For Their Potency As Cyclooxygenase-2 Inhibitors Nuzul Wahyuning Diyah; Anindi Lupita Nasyanska; Bambang Tri Purwanto; Siswandono Siswandono
Berkala Ilmiah Kimia Farmasi Vol. 7 No. 2 (2020): DESEMBER
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (805.626 KB) | DOI: 10.20473/bikfar.v7i2.29302

Abstract

A series of acyl salicylic acid derivatives were screened to investigate their analgesic activities and their potency as cyclooxygenase-2 (COX-2) inhibitors. Fourteen compounds (BS1–14) were assayed by acetic acid induced writhing test. Their ability for interaction with COX-2 was studied through a docking simulation at the COX-2 active site (PDB. 5IKQ). The results of the analgesic activity test gave 3 compounds that produce ED50< 0.39 mmol/kg body weight, lower than aspirin as a positive control. The compounds BS3 and BS4 showed excellent analgesic activity and the tert-butyl substituted molecule BS3 (O-(4-tert-butylbenzoyl)-salicylic acid analog) showed the highest analgesic activity with ED50 of 0.26 mmol/kg. Based on in silico molecular docking, it is known that almost all of the tested ligands (12 compounds) showed a higher binding affinity for COX-2 than meclofenamic acid which is a COX-2 inhibitory NSAID. The results of in vivo analgesic activity were justified with the outcome of in silico investigation. Molecular docking of acyl-salicylates confirmed in vivo experiments and it was found that BS3 was the most active compound as an analgesic agent and the most potent as a COX-2 inhibitor among the evaluated compounds.a
Validasi Metode KLT-Densitometri Pada Penetapan Kadar (-)-Epigallocatechin Gallate (EGCG) Dalam Teh Hijau Chusnul Chatimah; Sugijanto sugianto; Achmad Toto Poernomo
Berkala Ilmiah Kimia Farmasi Vol. 7 No. 2 (2020): DESEMBER
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (463.523 KB) | DOI: 10.20473/bikfar.v7i2.29303

Abstract

Latar Belakang Daun teh (Camellia sinensis L.) memiliki banyak manfaat untuk kesehatan. Daun teh hijau mengandung banyak senyawa polifenol dalam kadar tinggi, antara lain turunan catechin epicatechin (EC), epigallocatechin (EGC), epicatechin gallate (ECG) dan epigallocatechin gallate (EGCG). Komponen bioaktif yang paling dominan adalah EGCG. Sebuah metode TLC-Densitometry sederhana telah dikembangkan untuk penentuan (-)-Epigallocatechin gallate dalam produk teh hijau. Metode ini menggunakan silika gel F 254 sebagai fase diam dan fase gerak kloroform-asam asetat-asam format-isopropanol (16:2:2:8). Hasil Panjang gelombang maksimum terdeteksi pada 278 nm. Respon linieritas ditemukan (-)-Epigallocatechin gallate antara 0,5043 g–2,5215 g dengan regresi persamaan Y = 5409,8 X – 569,97 (r = 0,9996; Vxo= 3,94%). Metode divalidasi untuk menentukan deteksi tiruan (0,0188 g), kuantisasi batas (0,0627µg), akurasi (91,41%), presisi untuk standar EGCG (4,47%) dan presisi metode (7,01 %). Kesimpulan hasil ini menunjukkan bahwa EGCG dalam produk teh hijau dapat dideteksi dengan metode TLC-Densitometry. Dengan menggunakan metode, konsentrasi EGCG pada produk teh hijau adalah 3,33%.  

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