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Berkala Penelitian Hayati

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Published by Perhimpunan Biologi Indonesia Cabang Jawa Timur

ISSN : 08526834 EISSN : 2337389X DOI : https://doi.org/10.23869/bphjbr

Core Subject : Health, Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology

Berkala Penelitian Hayati is a half yearly international peer reviewed, an open access life science journal. The journal was published by The East Java Biological Society and formerly used the Indonesian language. The first edition of this journal is Vol 1 No 1 in June 1995. It was accredited by Ministry of Culture and Education. It continues recorded by Zoological Record by Thomson Reuters Clarivate Analytics since 2011. Since April 2012, the journal was changed into English. This journal is indexed by DOAJ, Crossref, Google Scholar, Academia.edu, and EBSCO Host. This journal publishes original research, applied, review article, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. This journal publishes original research, applied, review articles, and educational articles in all areas of biology. Authors are encouraged to submit complete unpublished and original works that are not under review in other journals. The journal scopes include, but are not limited to, the following topic areas including botany, zoology, ecology, microbiology, physiology, nanobiology, coastal biology, hydrobiology, neurobiology, genetics, developmental biology, biochemistry and molecular biology, biophysics, and life science.

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 507 Documents

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Characterization of biocontrol agents isolated from the leaves of mangroves grown in Sampiran Beach, North Sulawesi, Indonesia againts damping-off of cucumber Yuliar Yuliar; Suciatmih Suciatmih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 22 No 2 (2017): June 2017
Publisher : The East Java Biological Society

Bacterial biocontrol isolates MND61, MND63, and MND65 were originally isolated from mangrove leaves of Derris trifoliata Lour, Sonneratia alba J. Smith, and Avicennia marina (Forsk) respectively grown in Sampiran beach, North Manado, North Sulawesi island, Indonesia. They were evaluated on suppression of cucumber damping-off. Identification results showed that based on 16S rRNA sequence, MND61 isolate had 99% similarity to Kleibsiella pneumoniae ATTCC13884T, MND63 isolate had 98% similarity to Bacillus cereus NENA11, and MND65 isolate had 98% similarity to Bacillus subtilis jllsy. Results of greenhouse test showed that MND61 and MND63 suppressed Pythium aphanidermatum damping-off of cucumber significantly (P < 0.005) by 73-80%. Cocultures of these two biocontrol agent in potato dextrose broth also suppressed the pathogens population about 5-46 %. MND61 isolate produced indole acetic acid, in contrary to MND63 and MND65 isolates did not. The inhibitory assay effect of broth culture of MND61, MND63, and MND65 isolates to phytopatogenic fungi Fusarium oxysporum, P. aphanidermatum, P. ultimum, Rhizoctonia solani, Pyricularia oryzae and Xanthomonas oryzae in PDA plate indicated that two strains (MND63 and MND65) inhibited the growth of F. oxysporum, and R. solani growth, otherwise MND61 isolate did not inhibit the growth of any phytopatogenic assay.

Carbon storage of medium-sized tree: a case study on Dillenia collection in Purwodadi Botanic Garden Ridesti Rindyastuti
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 22 No 2 (2017): June 2017
Publisher : The East Java Biological Society

Dillenia is a medium-sized tree which has high species diversity in tropical regions especially in Southeast Asia. Dillenia in Purwodadi Botanic Garden are collected from native habitats in Java, Kalimantan, Sulawesi and Papua which planted on the area of 17 x 55 m2. The purpose of this re-search is to study the above ground carbon storage in Dillenia collection in Purwodadi Botanic Garden. Carbon storage estimation was established by measuring stem carbon stocks from plant collections with plant age ranging between 12-30 years. Twelve years old collection contributed carbon storage of 7.35 tonnes/ha for D. sumatrana. Twenty years old species had the lowest carbon storage of 2.17 kg/plant for D. serrata and the highest of 51.9 kg/plant for D. auriculata with a range of carbon storage of 3.47 to 41.072 tonnes/Ha. Thirty years old plant contributed 39.465 kg/plant and carbon storages of 63.14 tonnes/ha for D. serrata and 135.59 kg/plant and 216.94 tonnes/ha for D. philipinensis. Overall, Dillenia collections in Pur-wodadi Botanic Garden contributed 793.94 kg carbon storages, store carbon on average of 30.54 kg/plant and 46.46 tonnes/ha. The increase of carbon storage in the second 10 years was higher than in the first 10 years. It indicated that Dillenia had growth strategy in the early growth then alocated more mass after 10 years. Carbon storage of Dillenia was high and different in age. D. serrata, D. papuana and D. auriculata are recommended species as a priority in planting trees based on carbon sink.

Crab communities (Decapoda: Brachyura) in mangrove and estuaries in the Eastern Part of Lombok Island Dewi Citra Murniati
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 22 No 2 (2017): June 2017
Publisher : The East Java Biological Society

Mangroves and estuary ecosystem in East Lombok which are appointed as Local Marine Conservation Areas (Kawasan Konservasi Laut Daerah/KKLD) are Gili Lawang, Gili Sulat, Sambelia and Jerowaru districts. Inventory of aquatic fauna in mangroves and estuaries is one of the important components of the marine conservation program. The objective of this study is to determine crab species composition in mangroves and estuaries of eastern Lombok during the East Lombok KKLD program. Targeted area for this study includes Gili Lawang, Gili Sulat in southern Lombok Island, Sugian in Sambelia, Ekas Beach, Tanjung Luar and Seriwe in Jerowaru, and Kidang in western Jerowaru. The crab was collected at 0 m to 100 m of seashore by digging the burrow. The crab community recorded was analyzed for resulting the Diversity, Evenness and Similarity Index. Ten families with 35 species of crab were found from two ecosystem types, mangrove and estuary. Each location has different diversity of ecosystem and habitat. The highest diversity is recorded in Kidang and Seriwe, while the lowest diversity in Gili Sulat. Species diversity is associated with ecosystem and habitat type diversity. Kidang and Seriwe has the highest ecosystem and habitat diversity, while Gili Sulat has the lowest diversity. In addition, the population of Ocypodidae is accounted as the dominant crab family for which Austruca perplexa is the dominant species.

Effect of diazepam on kidney function and histological structure of white rat’s kidney Putu Gede Martha Setiawan; Woro Anindito Sri Tunjung; Luthfi Nurhidayat
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 22 No 1 (2016): December 2016
Publisher : The East Java Biological Society

The use of NAPZA (Narcotics, Alcohol, Psychotropic, and other addictive substances) in Indonesia keeps increasing. One type of frequently used NAPZAs is diazepam. Diazepam is a kind of sedative-hypnotics drug which belongs to benzodiazepine. The objective of this study was to determine kidney function by examining the level of urine ureum and creatinine also histological structure in rat after treated with diazepam. Rats were divided into one control group and three diazepam treatment groups which were solvent control (PEG 1%), treatment I (62.25 mg/kg BW), treatment II (83 mg/kg BW), and treatment III (124.5 mg/kg BW) of diazepam. These doses were based on LD50 in human. Rat urine was taken on the D0, D7, D14, D21, and D28. The ureum level was examined by ureumse-GLDH method and the creatinine level was analyzed by Jaffe method. The histological section was made by paraffin method with Hematoxylin and Eosin (HE) staining. Results showed that diazepam given to rat for 28 days led the in-crease of urine ureum and creatinine levels compare to control group. Furthermore, histological damages of the kidney also detected including cellular injuries, both reversible and irreversible injuries, congestion, hemorrhage, and glomerular damage. In conclusion, the use of diazepam for 28 days affected kidney function in rat.

Trees structure and composition on logged over forest at Kopiyo Meratus, Hulu Sungai Tengah District, South Kalimantan Asep Sadili
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 22 No 1 (2016): December 2016
Publisher : The East Java Biological Society

Natural forest area in Kalimantan has changed drastically. The aim of this study was to reveal the diversity of tree in Kopiyo Forest of Meratus after termination of Forest Concession was over. Study was carried out at two different observation sites with square method (slope and drier especially). The obtained data was varied, which diversity in the sapling group in two site was 32 species, 28 genera, 25 families, and density of 285 individu-als/ha, with basal area ± 9.50 m2/ha. Whereas, diversity in the tree group in two site was 34 species, 28 genera, 23 families, and density of 485 individual/ha, basal area ± 32.85 m2/ha. Gordonia cf. borneensis was the most dominant species of sapling group in site I and II, and Macaranga hypoglauca was the most dominant species of the tree group in site I and II. Euphorbiaceae, Dipterocarpaceae, Sapindaceae, Myrtaceae, and Rubia-ceae were mostly found in observation sites. Shorea parvifolia, S. pinanga, and S. Smithiana were species with endanger status.

Activity test of various mangosteen (Garcinia mangostana) pericarp extract fractions to decrease fasting blood cholesterol levels and lipid peroxidation activity in diabetic mice Saikhu Akhmad Husen; Dwi Winarni; Firas Khaleyla; Septian Hary Kalqutny
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 22 No 1 (2016): December 2016
Publisher : The East Java Biological Society

The objectives of this study were to determine the effect of various fractions of mangosteen (Garcinia mangostana) pericarp extract to the changes of the fasting blood cholesterol and serum malondialdehyde (MDA) levels on diabetic mice (Mus musculus). Thirty 3-4 months old male mice strain BALB/c, weight 20-30 g were divided into six groups. The first group was KN as a non diabetic control group, KD as a diabetic control, KM as a group of diabetic mice treated with metformin, and NP, SP, and P as the treatment groups that were treated by using three different fractions from mangosteen pericarp extract, non polar, semi polar, and polar respectively. The induction of Diabetes mellitus was done by the injection of STZ, and the mice were given a high fat diet treatment to induce the hiperlipidemia condition using lard for three weeks. The blood cholesterol levels were measured in all groups before and after the injection of lard, and day 1, 7, and 14 of treatment as well. The serum MDA level as the indicator of lipid peroxidation were measured by using QuantiChrom TBARS Assay Kit (DTBA-100). The data of cholesterol levels were statistically analyzed by t-test, while the data of serum MDA levels were analyzed by variance analysis followed by Duncan test. The results showed that the polar fraction of mangosteen pericarp had effect to decrease the fasting blood cholesterol level in mice, however the non polar and semi polar fraction had no simmilar effect. All of the fractions has shown significant effect to decrease the serum MDA level in mice.

The growth of seedlings of rhizophoraceae in the nursery utilizing fresh water Sarno Sarno; Moh. Rasyid Ridho
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 22 No 1 (2016): December 2016
Publisher : The East Java Biological Society

The recovery of the mangrove areas that have been degraded needs mangrove seedlings in large quantity. A mangrove nursery is generally located on the land close to the planting site and brackish water. The mangrove nursery by means of fresh water is an innovation in the provision of seeds. The objective of this experiment was to determine the growth of seedlings of Rhizophoraceae in fresh water based on the parameters of the number of leaves and the height of the shoots. The method of breeding utilizes fresh water and to keep the water remains available the water circulation is regulated. The results of the observations show that the number of leaves of Bruguiera gymnorrhiza has the number of pairs of leaves compared with Rhizophora apiculata dn R. mucronata. The average height of the shoots up to the age of 5 months after planting, the highest seedlings are R. mucronata seedlings. In general, mangrove nursery with fresh water can be utilized as a means for providing seedlings for rehabilitation of mangrove.

Effect of organic matter and Si liquid fertilizer on growth and yield of sugar cane Djajadi Djajadi; Sulis Nur Hidayati; Roni Syaputra
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 22 No 1 (2016): December 2016
Publisher : The East Java Biological Society

Sugarcane is known to absorb more Si than any other nutrient from the soil; therefore continuous cropping of the plant at the same soil would bring consequences of more Si and organic matter depletion. Silicon (Si) is considered as a beneficial nutrient for sugarcane production while organic matter is well known as soil amendment. Field study was carried out to know the effect of organic and Si liquid fertilizer on growth, Si and N uptake, and yield of cane variety of PSBM 901. The study field was located at Kempleng village, Purwoasri, East Java and the study was done from May 2013 up to September 2014. Split plot design with three replicates was employed to arrange treatments. Organic matter types (no organic matter, Crotalaria juncea and manure) were set as main plots while Si liquid fertilizer concentration (0, 15% Si and 30% S) were arranged as sub plots. C juncea was planted at 15 days before planting of sugar cane, and after 35 days the C juncea were chopped and mixed into the soil. Manure was added one week before sugar cane was planted. Si liquid fertilizer was sprayed to the whole part of sugar cane plant at 30 and 50 days after sugar cane was planted. All treatments received basal fertilizer of 800 kg ZA/ha, 200 kg SP 36/ha and 300 kg KCl/ha. Results showed that interaction between organic matter and Si liquid fertilizer significantly affected on Si and N absorption, length of stem, yield and rendement of sugar cane. Addition of manure and followed by spraying of 30% Si liquid fertilizer gave the highest value of S and N absorption (869 g SiO2/plant and 720 g N/plant), cane yield (155.74 tons/ha) and rendement (8.15%).

Growth and accumulation of flavan-3-ol in Camellia sinensis through callus culture and suspension culture method Sutini Sutini; Susilowati Susilowati; M. Rasjad Indra; Djoko Agus Purwanto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 22 No 1 (2016): December 2016
Publisher : The East Java Biological Society

This study was aimed to assess flavan-3-ol biomass in C. sinensis through callus cultures and suspension cultures derived from leaf explants. Callus initiation of both cultures were using Murashige and Skoog medium were enriched with plant growth regulators Naphthalene Acetic Acid 3.0 mg/L and kinetin 2.0 mg/L. The procedures in this study were: (1) callus initiation by cutting the leaves of C. sinensis shoots then planted on Murashige and Skoog medium that were enriched with plant growth regulators, (2) sub callus culture on fresh medium that enriched with the same growth regu-lators, (3) suspension culture initiation of liquid callus, (4) growth examination of callus and suspension cultures in week 12, (5) examination of qualitative-quantitative content of flavan-3-olin suspension cultures at week 4. The results show that suspension cultures contain biomass flavan-3-ol that increase in the same manner of the increase of callus age and weight.

Flowering of Areca catechu in Bogor Botanic Gardens Angga Yudaputra; Rizmoon N. Zulkarnaen; Irvan F. Wanda; Joko R. Witono
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 22 No 1 (2016): December 2016
Publisher : The East Java Biological Society

Areca catechu is a species of palms that widely distributed in Indonesia. This palm has unique characteristics and important roles of a tropical forest ecosystem. Indonesian local tribes used seed of this palm for traditionaly medicinal purposes. Information regarding of flowering of A.catechu is valuabe to support a genetic improvement program. Currently, the flowering information about this palm is still lack, thereby study that related with flowering of A. catechu is important to be conducted. This study aimed to provide information about the flowering of A.catechu species. The observation was started with randomly selection of three individuals A. catechu from the Moluccas island. There were 3 main observations which done in this study, included the observation of flowering phases period, morphological changes of each phases, and the micro-climate factors. Flowering A. catechu was categorized into 4 phases, including initiation, bud towards anthesis, anthesis and young fruit to maturity. Each of flowering phases have a different time. The flowering phases were not directly influenced by micro-climate factors. Trigona laeviceps was the most dominant insect visitor and wind was an abiotic factors that influenced the flowering of A. catechu.

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Sutiman Bambang Sumitro

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berkalahayati@yahoo.com

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+62341570631

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Abstract

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Sutiman Bambang Sumitro

Contact Email berkalahayati@yahoo.com

Phone +62341570631

Journal Mail Official wulidanisa@berkalahayati.org

Editorial Address Jalan Surakarta No. 5 Malang, Indonesia

Location Unknown, Unknown INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Sutiman Bambang Sumitro

Contact Email
berkalahayati@yahoo.com

Phone
+62341570631

Journal Mail Official
wulidanisa@berkalahayati.org

Editorial Address
Jalan Surakarta No. 5 Malang, Indonesia

Location
Unknown,

Unknown

INDONESIA