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BIOVALENTIA: Biological Research Journal

biov
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Published by Universitas Sriwijaya

ISSN : - EISSN : 24771392 DOI : https://doi.org/10.24233/biov

Core Subject : Health, Science, Agriculture, Social,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology

BIOVALENTIA: Biological Research Journal aims to review and publish the Biological research results from researchers. The range of articles which BIOVALENTIA: Biological Research Journal publishing is the research results in Biology science and applied Biology. Biology science is included Ecology, Biosystematics, Physiology, Developmental Biology, Microbiology, Genetics. Applied Biology is included Biotechnology, and the sciences derived from Biology science.

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 136 Documents

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EFFECT OF GIVING MIXED INSECTICIDE CARBOFURAN IN COW FECES TOWARDS CONSUMPTION RATE AND ASSIMILATION EFFICIENCY EARTHWORM Pheretima javanica Gates Nofyan, Erwin; Lamin, Syafrina; Patriot, Innocenthya Tygra
BIOVALENTIA: Biological Research Journal Vol. 2 No. 2 (2016)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Research about â€œGiving a mixture of Insecticide Carbofuran in cow feces to the Rate of Consumption and the Efficiency of Absorption on Land Worm Pheretima javanica Gates was held on June to August 2016 at Animal Physiology Laboratorium, Biology Department, Faculty of Mathematics and Science, Sriwijaya University, Indralaya, Ogan Ilir, South Sumatera. The purpose of this research is to learn the effect of Insecticide Carbofuran to the rate of consumption and the efficiency of absorption on land worm Pheretima javanica Gates. Contribution of this research gives the information to farmer about the effect of insecticide carbofuran to non-target animal, especially to land worm Pheretima javanica Gates. This research used Completely Randomized Design with 6 treatments and 5 times repetition. Treatment that was given to sample are the insecticide carbofuran with concentration ofÂ 0 % (control); 0.1% ;Â 0.2 % ; 0.3 % ;Â 0.4 % ; 0.5 %. Data analysis was using Varians Analysis. If there was real difference then data analysis continued with The Duncan Test on level of confidence ofÂ 95%. The results of this research show us that several concentration of insecticide carbofuran have the real effect to the average of consumption rate and the efficiency of absorption. The lowest average of consumption rate on land wormÂ Pheretima javanica is on concentration of 0,5 % (0.23 Â± 0.02Â mg/g day) and the highest average of consumption rate on land wormÂ Pheretima javanica is on concentration of 0% (control) (2.53 Â± 0.05 mg/g day). The lowest average of absorption efficiency on land wormÂ Pheretima javanica is on concentration of 0 % (control) (40.78Â Â± 2.56 % )and the highest average of absorption efficiency on land wormÂ Pheretima javanica is on concentration of 0,5 % (70.76Â Â± 3.67 %). Â Keywords: carbofuran, the rate of consumption, the efficiency of absorption,Â Pheretima javanica Gates.

CONDITION OPTIMIZATION PRODUCTION XYLANASE THERMOSTABLE BY Bacillus licheniformis TS10 USING SUBSTRATE OIL PALM EMPTY FRUIT BUNCHES Safitri, Ayu; Muharni, -; Kusumawati, Heni Yohandini
BIOVALENTIA: Biological Research Journal Vol. 3 No. 1 (2017)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Optimization conditions for thermostable xylanase production ofÂ Bacillus licheniformis TS10 by using substrate oil palm empty fruit bunchesÂ has been conducted from November 2015 to January 2016 in the Laboratory of Genetics and Biotechnology, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Sriwijaya, Indralaya. The aims of this study was to determine the potential of oil palm empty fruit bunches ( EFB ) as a substrate for the production of thermostable xylanase by Bacillus licheniformis TS10 and the optimum conditions of temperature, pH and substrate concentration in the fermentation process. The research method is to make the growth curve and the curve of Bacillus licheniformis TS10 enzyme production on EFB substrate. The number of bacterial cells was determined by using a UV-VIS spectrophotometer and total plate count (TPC) through a standard curve. Optimization performed at various pH (5, 6, 7, 8, 9), temperature (50 Â°C, 60 Â°C, 70 Â°C, 80 Â°C) and substrate (1%, 2%, 3%, 4%) , Test activity at each pH, temperature and substrate usingÂ methods DNS by measuring enzyme activity based on the reducing sugar released by the substrate by using dinitrosalicylic acid (DNS). Based on the results of this research showed that oil palm empty fruit bunches (EFB ) has potential as a substrate for the production of thermostable xylanase by Bacillus licheniformis TS10, thermostable xylanase production of Bacillus licheniformis TS10 on the substrate EFB has an optimum condition at pH 6, 80 Â°C and the substrate concentration of 4%.

DIVERSITY OF WATER ENVIRONMENT GASTROPODA IN A EMPAYANG-KASAP RIVER IN THE DISTRICT LAHAT Hecca, Desven; Hidayat, Saleh; Dewiyeti, Susi
BIOVALENTIA: Biological Research Journal Vol. 3 No. 1 (2017)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

This research has been conducted with the tittle the diversity of gastropods on the river water environment Empayang Lahat regency of South Sumatra province. The aim of this research species diversity of gastropods contained the region. This research was implemented conducted in October 2015, a river Empayang Kasap Sukajadi Lahat regency. The methods of this research is purposive random sampling method with 5 sampling stations, each station is divided into three substations (edges first, middle, edge 2) was determined by linear transects (line transect). The data of the sample was taken in the morning and afternoon. The results of this research were obtained 8 species of gastropods (Melanoides granifera, Elimia acuta, Brotia tetidinaria, Lymnae stagnalist, Lymnae rubiginosa, Pomacea canaliculata, Pila polita and Menetus sp) with 7 genus, and of the six tribes (families) of 4 order. The total index of the diversity in the river Empayang Kasap Sukajadi Lahat district was of 2.09 to 2.46. The highest levels of diversity found in the station V (2.46) and the lowest at station I (2.09). While the value of dominance index (C) with a range of 0.17 to 0.26 at five stations in the lower state, the highest level of dominance index at station I (0.26) and the lowest at station III (0.17). River water quality conditions greatly affect the existence of gastropods.

THE ANTIBACTERIAL ACTIVITY OF WATER APPLE LEAVES ACTIVE COMPOUND (Syzygium zeylanicum) AGAINST Escherichia coli AND Staphylococcus aureus Hamidah, -; Salni, -; Tanzerina, Nina
BIOVALENTIA: Biological Research Journal Vol. 3 No. 1 (2017)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Escherichia coli is one of the bacteria that cause infections in the human digestive tract such as diarrhea, while Staphylococcus aureus is one of the bacteria that cause infections in the skin injury such as boils and pimples. This study used Syzygium zeylanicum leaves because it has potential as a antibacterial because it contains active compounds. This study aimed was determine the antibacterial activity of the fraction and the active compound in Syzygium zeylanicum leaves against Escherichia coli and Staphylococcus aureus. Research conducted on November 2015 to January 2016. The method used in this research were extraction by maceration, fractionation by liquid fractionation, antibacterial activity test, and determination of minimum inhibitory concentration with the diffusion method and isolation of active compounds by column chromatography method. The bacteria used in this test are Escherichia coli and Staphylococcus aureus. Data are presented in tabular form based on the average value of the inhibition diameter and deviation standard. The results of this research showed the water methanol active fraction against the bacteria that used in this test. The methanol water fraction had obtained one antibacterial compound in bottle 1,3,5 which shows the value of tannin Rf 0,416. The minimum inhibitory concentration of water methanol of water apple leaves is 1000 Âµg/mL for Escherichia coli and 500 Âµg/mL for Â Staphylococcus aureus. The minimum Â inhibitory concentration of the activeÂ compound Â to Â Escherichia Â coli Â and Â Staphylococcus Â aureus Â in Â 500 Â Âµg/mL. Â The fraction and the active compound of water apple leaves have an antibacterial activity with Escherichia coli and Staphylococcus aureus and the active compound is tannin.

HABITAT OPTIMALIZATION OF CAPTIVE DEER PT. PUSRI PALEMBANG Rahmania, Nadya; Yustian, Indra; Setiawan, Arum
BIOVALENTIA: Biological Research Journal Vol. 3 No. 1 (2017)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Deer is one of the species that going through a reduction of population because its being used as the target of illegal hunting. To prevent the reduction in population, the efforts to save them with conservation method both in-situ or ex-situ has been done. Captive deer in PT. Pusri Palembang is the only captive deer which located in urban areas of Palembang and carrying out an ex-situ conservation effort. This research aimed to analyze the habitat condition such as daily feed, water needed, and shelter, to know about the level of palatability and also to analyze the habitat condition as a basic of captive deer management towards an optimalization. The research has been done in January 2015. The interview method, literature study and field observation are used to get the data about population and habitat condition in captive deer PT. Pusri Palembang. It can be concluded that the habitat condition in the captive deer PT. Pusri Palembang still quite good, and 1,5 hectare areas still be able to maintain the total of 44 deers (22 sambar deers and 22 chital deers) in 2015.Key words: sambar deer, chital deer, captivity, ex-situ conservation, PT Pusri Palembang.Â

HORMESIS PHENOMENA OF SOME PARAMETER ASPECT IN USAGE OF WATER LETTUCE (Pistia stratiotes L.) FOR PHYTOREMEDIATION PROCESS OF PETROLEUM LIQUID WASTE Marisa, Hanifa; Estuningsih, Sri Pertiwi
BIOVALENTIA: Biological Research Journal Vol. 3 No. 1 (2017)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

The research about potential test of water lettuce (Pistia stratiotes) in order of phytoremediation of petroleum liquid waste had been done during June untill December 2012 at Microbiology Laboratory, Department of Biology, Faculty of Science, The University of Sriwijaya, Indonesia. Completely Randomized Design was used in the experiment while the concentration of liquid waste as treatment were: 0, 15, 30, 45, 60 and 75 %. Each treatment were replicated 4 times. Three parameter was measured; Total Petroleum Hydrocarbons (TPH) reduction percentage, efficiency of phytoremediation and number of tillers. Regression analisys were used for the data and two of parameters show the hormesis phenomena, were; efficency of phytoremediation and number of tillers. The highestÂ peak effect of treatment for efficiency of phytoremediation and number of tillers was on 45 %. TPH reduction percentage was the only one parameter that shows linier regressionÂ Keywords hormesis, Pistia stratiotes, TPH, phytoremediation, tiller

ANTIBACTERIAL ACTIVITY STUDY OF ACTIVE FRACTION FROM CHICK WEED PLANTS (Ageratum Conyzoides L.) AGAINST Bacillus Subtilis AND Vibrio Cholerae Anggara, Ratih; Munawar, -; Elfita, -
BIOVALENTIA: Biological Research Journal Vol. 3 No. 1 (2017)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

The purpose of this research to determine the fractions of Chick Weed which has strong antibacterial activityÂ against bacteria test categories of Bacillus subtilis and Vibriocholerae.determine the value of the minimum in hibitory concentration(MIC) of the active fraction antibacterial Chick Weed.This research was carried out in August up to November 2016. The method used in this studyÂ were extracted by maceration, fractionation by liquid-liquid fractionation, separation by column chromatography fractions, antibacterial activity test by theKirby-Bauermethod, while the determination of minimum in hibitory concentration by dilution broth,with test bacteria Bacillus subtilis and Vibriocholerae.The data presented in tabular form based on the average value and percent.The results of this study showed that the methanol extract Chick Weed active against test bacteria Bacillus subtilis and Vibrio cholerae. Fractionation which has strong category to standard antibiotics are methanol fraction by fraction column S4.The concentration MIC1000;500;250;125;62.5;31.2515.62; 7.81 ppm. The minimum in hibitory concentration column fractions S4 to test bacteria Vibrio cholerae of 62.5 ppm gives half the antibacterial activity of the antibacterial activity of standard antibiotics streptomycin and penicillin,tetracycline while giving a quarter activity. It can be concluded that the active fraction of methanol extractisa methanol fraction by fraction column S4 to test bacteria Vibrio cholerae.Keywords: Chick Weed, Minimum Inhibitory Concentration (MIC), active compound, Bacillus subtilis, Vibriocholera.

VEGETATION ANALYSIS ON RECLAMATION AREA OF COAL MINE OF PT. BUKIT ASAM TANJUNG ENIM, SOUTH SUMATERA Prasetyo, Rendra Bayu; Marisa, Hanifa; Sarno, -
BIOVALENTIA: Biological Research Journal Vol. 3 No. 1 (2017)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

The Research of Vegetation Analysis on Reclamation Area of Coal Mine of PT. Bukit Asam Tanjung Enim, South Sumatera was aimed to determine the types of vegetation that composing reclamation area was found in PT. Bukit Asam in terms of structure and composition. The research has been done in November until Desember 2014, in four reclamation area at PT. Bukit Asam, namely the area of reclaimed the Muara Tiga Besar Selatan, Mahayung, Klawas Timur, and the Muara Tiga Selatan having different strata age. the vegetation data includes rate of growth seedlings, saplings, poles, and tree obtained through transek method in the form plot lane. In each transect made a plot in size 20x20 m for tree level, 10 x 10 for poles level, 5x5 for saplings level, and 2x2 m for seedling and cover plants level. The results of this research, in the Muara Tiga Besar Selatan area was obtained the vegetation seedlings-cover plants and saplings level, each of 17 and 7 species. In the area Mahayung are only found level vegetation of seedlings-cover plants as many of 13 species. For the area Klawas Timur and Muara Tiga Besar Selatan was obtained growth level of seedlings-cover plants, saplings, poles, and tree each of 20, 4. 3, dan 2 species for Klawas Timur area, while on Muara Tiga Selatan area each obtained 19, 8, 7, and 3 species.The diversity index of species, overall in all area of reclamation and rate of growth included in the low category with value 0 â‰¤ Hâ€™ â‰¤ 2.Key words : PT. Bukit Asam, Vegetation Analysis, Reclamation, Transect, Diversity Index

CONCENTRATIONS TEST OF METHANE GAS FROM SWAMP FORAGE SILAGE WITH IN VITRO METHOD Sarno Sarno; Moh. Rasyid Ridho; Dwi Puspa Indriani; Harmida Harmida; Adelia Rizki Pancasari
BIOVALENTIA: Biological Research Journal Vol. 3 No. 2 (2017)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

Aims of this study to concentration test of methane gas from swamp forage silage with in vitro methods.This study used Completely Randomized Design with 3 treatments and each treatment consisted of 5 replications. The treatments used are as follows: P1 (100% kumpai tembaga grass (Hymenachne acutigluma)), P2 (50% kumpai tembaga grass (Hymenachne acutigluma) + 50% kemon air (Neptunia oleracea lour)), P3 (100% kemon air ( Neptunia oleracea lour)). The parameters observed N-Ammonia, total gas production, methane gas concentrations in vitro and VFA partially. The results of variance showed that treatment significantly different (P <0.05) with methane concentration in vitro with highest gas concentration treatment of silage made from 50% kumpai tembaga grass (Hymenachne acutigluma) and 50% kemon air (Neptunia oleracea lour).Keywords: Concentration, Methane, Silage Forage Swamp, In Vitro.

EFFECT OF LIME APPLICATION ON INDIGENOUS NITROGEN-FIXING BACTERIA IN TIDAL SOILS MANAGED FOR THAN 30 YEARS Ridwan, Rahmawati; Budianta, Dedik; Widjajanti, Hary
BIOVALENTIA: Biological Research Journal Vol. 3 No. 2 (2017)
Publisher : Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University

The study was conducted to determine the effect of land typology and lime on the population of indigenous nitrogen-fixing bacteria and soil pH, and to analyze the effect of soil typology interaction and lime on the population of indigenous nitrogen-fixing bacteria and soil pH. Sampling of soil in tidal land of Mulya Sari and Banyu Urip Village of Banyuasin Regency, South Sumatera. Then the sample was studied at the Laboratory of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, University of Sriwijaya, Inderalaya. During January-May 2017. Based on the results of the study known the soil typology of the total population of nitrogen-fixing bacteria, the highest population of nitrogen inhibitor bacteria in the second week of soil typology D was 6.0x107 and the highest pH of 5.18 in typology B on week Third, the lime treatment that affected the population of indigenous nitrogen-fixing bacteria, obtained the population of bacteria as much as 9.3x105 in the second week and the highest soil pH 4.93 in the second week, while the results of the interaction between soil typology and lime treatment only affect the population of bacterial inhibitor Indigenous nitrogen, with bacterial population of 3.5x107 in soil typology D in the second week, but this interaction did not significantly affect soil pH, with the highest pH measurement of 5.33 on the soil typology D.Keywords: nitrogen-fixing bacteria, tidal land, lime treatment

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Editorial Address Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University Palembang-Prabumulih Road KM 32 Indralaya, Ogan Ilir, South Sumatera 30662 Indonesia

Location Kab. ogan ilir, Sumatera selatan INDONESIA

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Contact Name
Enggar Patriono

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epatriono@unsri.ac.id

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+62711 580306

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biovalentia@unsri.ac.id

Editorial Address
Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University Palembang-Prabumulih Road KM 32 Indralaya, Ogan Ilir, South Sumatera 30662 Indonesia

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Kab. ogan ilir,

Sumatera selatan

INDONESIA