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Contact Name
Heri Setiyo Bekti
Contact Email
ejournalskalahusada@gmail.com
Phone
+6281703709024
Journal Mail Official
ejournalskalahusada@gmail.com
Editorial Address
Jalan Sanitasi No.1 Sidakarya 80224 Denpasar, Bali - Indonesia
Location
Kota denpasar,
Bali
INDONESIA
JURNAL SKALA HUSADA: THE JOURNAL OF HEALTH
ISSN : 1693931X     EISSN : 25803700     DOI : https://doi.org/10.33992/jsh:tjoh
Core Subject : Health,
Jurnal Skala Husada merupakan media publikasi karya ilmiah para dosen di lingkungan Politeknik Kesehatan yang ada di Indonesia, khususnya karya ilmiah di bidang teknologi kesehatan. Jurnal Skala Husada terbit setiap 6 bulan, atau terbit 2 kali dalam setahun.
Articles 65 Documents
Pengaruh Promosi Kesehatan Melalui Video Edukasi Terhadap Perubahan Pengetahuan dan Sikap Siswa Sekolah Dasar Tentang Pengelolaan Sampah Plastik Ni Kadek Setyaningrum; M. Choirul Hadi; Anysiah Elly Yulianti
Jurnal Skala Husada : The Journal of Health Vol 18, No 2 (2021): Jurnal Skala Husada: The Journal of Health
Publisher : Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (381.333 KB) | DOI: 10.33992/jsh:tjoh.v18i2.1846

Abstract

ABSTRACTPopulation growth and changes in community consumption patterns lead to an increase in volume, type and characteristics of waste. If there is a change in the environment around humans, it’s can change the environmental conditions. The method of health promotion with the video screening method with the design of One Group Pretest Posttest to change behavior so it can get increased knowledge and attitudes. The results of research conducted at SD Negeri 8 Sumerta Denpasar for education about waste management using paired t-test obtained pretest scores of knowledge of grade IV and V students of 5.62 and 6.15. As for the results of the pretest attitudes of class IV and V students namely 5.59 and 6.70. For the posttest value of students' knowledge in grades IV and V, they are 7.59 and 7.88. While the posttest scores of students attitudes in grades IV and V are 6.21 and 8.48. Paired t-test results obtained sig (2-tailed) (0,000) alpha (0.05) then H0 is rejected and HI is accepted, it’s means there are differences students' knowledge and attitudes about waste management before and after getting health promotion video in SD Negeri 8 Sumerta Denpasar.ABSTRAKPertumbuhan penduduk dan perubahan pola konsumsi masyarakat menyebabkan peningkatan volume, jenis dan karakteristik sampah. Jika terjadi perubahan lingkungan di sekitar manusia, maka dapat mengubah kondisi lingkungan. Metode promosi kesehatan dengan metode pemutaran video dengan desain One Group Pretest Posttest untuk mengubah perilaku sehingga bisa mendapatkan peningkatan pengetahuan dan sikap. Hasil penelitian yang dilakukan di SD Negeri 8 Sumerta Denpasar untuk pendidikan tentang pengelolaan sampah dengan menggunakan uji-t berpasangan diperoleh nilai pretest pengetahuan siswa kelas IV dan V sebesar 5,62 dan 6,15. Adapun hasil pretest sikap siswa kelas IV dan V yaitu 5,59 dan 6,70. Untuk nilai posttest pengetahuan siswa kelas IV dan V masing-masing sebesar 7,59 dan 7,88. Sedangkan nilai posttest sikap siswa kelas IV dan V adalah 6,21 dan 8,48. Hasil uji t berpasangan diperoleh sig (2-tailed) (0,000) alpha (0,05) maka H0 ditolak dan HI diterima, artinya ada perbedaan pengetahuan dan sikap siswa tentang pengelolaan sampah sebelum dan sesudah mendapatkan video promosi kesehatan di SD Negeri 8 Sumatera Denpasar. 
FAKTOR-FAKTOR YANG MEMPENGARUHI PARTISIPASI MASYARAKAT DALAM PENGELOLAAN SAMPAH PADAT DI DENPASAR TIMUR Dewa Ayu Posmaningsih
Jurnal Skala Husada : The Journal of Health Vol 13, No 1 (2016): Jurnal Skala Husada: The Journal of Health
Publisher : Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (104.238 KB) | DOI: 10.33992/jsh:tjoh.v13i1.79

Abstract

Abstract:  Urban waste management also has the driving factors and inhibitors in an effort to increase community participation in waste management. The research objective is to find out how much the relationship of factors predisposing, enabling and reinforcing community participation in solid waste management. The design of this study include observational research with cross sectional. The sample size is 180 housewife and the sample was collected through stratified random sampling. The results showed there is a relationship of knowledge and attitudes towards participation in waste management with the knowledge of determination of 20.6% and 24.9% for attitude determination. Facilities management of community participation in waste management with the determination of 36.2%. Relationship local agencies, community leaders and economic benefits of community participation in waste management by local institutions determination of 10.3%, determination of public figures by 8.5%, and the determination of the economic benefits of 35.9%. Facility factors, economic benefits, local institutions, knowledge and attitudes to contribute jointly to the participation of the determination of 65.1%. The conclusions of this study is a factor predisposing, enabling and reinforcing related to public participation. Factors of knowledge, attitudes, facilities, local agencies, and economic benefits together to contribute to community participation. It is recommended that local governments provide guidance to local agencies in participatory planning of environmentally sound and sustainable solid waste management.Keywords: Predisposing, enabling, reinforcement factors, community  participation Abstrak: Pengelolaan sampah perkotaan juga memiliki faktor pendorong dan penghambat dalam upaya untuk meningkatkan partisipasi masyarakat dalam pengelolaan sampah. Tujuan penelitian ini adalah untuk mengetahui seberapa besar hubungan faktor predisposisi, pendukung dan pendorong partisipasi masyarakat dalam pengelolaan sampah. Desain penelitian ini termasuk penelitian observasional dengan cross sectional. Ukuran sampel adalah 180 ibu rumah tangga dan sampel dikumpulkan melalui stratified random sampling. Hasil penelitian menunjukkan ada hubungan pengetahuan dan sikap terhadap partisipasi dalam pengelolaan sampah dengan pengetahuan. Kesimpulan dari penelitian ini adalah faktor predisposisi, pendukung dan pendorong terkait dengan partisipasi masyarakat. Faktor pengetahuan, sikap, fasilitas, lembaga lokal, dan manfaat ekonomi bersama-sama untuk memberikan kontribusi terhadap partisipasi masyarakat. Kepada pemerintah daerah disarankan memberikan bimbingan kepada lembaga lokal dalam perencanaan partisipatif dari pengelolaan sampah yang berwawasan lingkungan dan berkelanjutan.Kata kunci: Faktor predisposisi, pendukung, penguat, partisipasi masyarakat
Tinjauan Rhodamin B Pada Gula Kapas Arum Manis Di Lapangan Puputan Badung Kadek Ayu Candra Duhita; I Gusti Ayu Sri Dhyanaputri; IGA. Dewi Sarihati
Jurnal Skala Husada : The Journal of Health Vol 17, No 2 (2020): Jurnal Skala Husada: The Journal of Health
Publisher : Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (165.841 KB) | DOI: 10.33992/jsh:tjoh.v17i2.2065

Abstract

ABSTRACTRhodamine B is a synthetic dye that is commonly used as a textile dye and banned its use in food products. The use of rhodamine B in food for a long time can lead to liver dysfunction and cancer, and when exposed to large amounts of rhodamine B and then in a short time there will be symptoms of acute poisoning rhodamine B. There were some cases the addition of rhodamine B in food. One is the addition of rhodamine B in cotton candy. The purpose of this study is to determine the contents of rhodamine B dye in cotton candy around Puputan Badung I Gusti Ngurah Made Agung field. Objectives to be achieved in this research is to determine the presence of rhodamin B dye content in cotton candy around Puputan Badung I Gusti Ngurah Made Agung field. Methods this study was an observasional study. Data collected through the sampling stage then testing with the laboratory test methods Thin Layer Chromatography and the results were analyzed descriptively. Results total of 9 sampels tested, 4 sampel (44.5%) cotton candy were positive for rhodamin B. Conclusions rhodamine B dye was found in four sampel of cotton candy around  Puputan Badung I Gusti Ngurah Made Agung field. Cotton candy found positive rhodamine B has a bright pink color.ABSTRAKRhodamin B merupakan pewarna sintetis yang umum digunakan sebagai pewarna tekstil dan dilarang penggunaannya dalam produk makanan. Penggunaan rhodamin B dalam makanan dalam waktu lama dapat menyebabkan gangguan fungsi hati dan kanker, dan bila terkena rhodamin B dalam jumlah besar maka dalam waktu singkat akan timbul gejala keracunan rhodamin B akut. rhodamin B dalam makanan. Salah satunya adalah penambahan rhodamin B pada permen kapas. Tujuan dari penelitian ini adalah untuk mengetahui kandungan zat warna rhodamin B pada permen kapas di sekitar Lapangan Puputan Badung I Gusti Ngurah Made Agung. Tujuan yang ingin dicapai dalam penelitian ini adalah untuk mengetahui adanya kandungan zat warna rhodamin B pada permen kapas di sekitar Lapangan Puputan Badung I Gusti Ngurah Made Agung. Metode penelitian ini adalah penelitian observasional. Data dikumpulkan melalui tahap sampling kemudian pengujian dengan metode uji laboratorium Kromatografi Lapis Tipis dan hasilnya dianalisis secara deskriptif. Hasil uji total 9 sampel, 4 sampel (44,5%) permen kapas positif rhodamin B. Kesimpulan pewarna rhodamin B ditemukan pada empat sampel permen kapas di sekitar Lapangan Puputan Badung I Gusti Ngurah Made Agung. Permen kapas yang ditemukan positif rhodamin B memiliki warna pink cerah.
PROFIL KETAHANAN PANGAN KABUPATEN JEMBRANA 2017 Hertog Nursanyoto
Jurnal Skala Husada : The Journal of Health Vol 15, No 1 (2018): Jurnal Skala Husada: The Journal of Health
Publisher : Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (109.59 KB) | DOI: 10.33992/jsh:tjoh.v15i1.219

Abstract

Abstract. The agricultural and plantation sectors are still the main economic support in Jembrana. Potential commodities that include coconut, coffee, cloves, cacao, and vanilla. Although Jembrana has been known as plentiful food production area, but food security study in Jembrana never done. This research is a cross sectional study conducted for 6 months in 10 selected villages namely Warnasari and Tuwed villages in Melaya; Tegal Badeng Barat and Baler Bale Agung in the Negara; Budeng and Yeh Kuning in Jembrana; Pohsanten and Delod Berawah in Mendoyo; and also Gumrih and Pulukan in Pekutatan. From each village was selected by simple random sampling of 20 households to obtain the total sample as a whole, amounting to200 households. From the result, it is known that the energy accessibility of Jembrana population reach 3669,64 kcal/capita/day, or about 166,8% AKE. As for the accessibility of protein reached 160,96 g/capita/day or nearly three-fold (282.39% AKP). The energy consumption is 2174,37 kcal/capita/day (101,13% AKE). While the protein consumption was 71.65 g/capita/day (125,7% PPA) with an average food desirable pattern  score (PPH) of 90,06. It can be concluded that the food consumption pattern of Jembrana Regency in 2017 quantitatively has been good, but qualitatively still not fulfill the national target. Some current problems and possibilities that occur in the future namely: a) The energy consumption, and especially the protein consumption of Jembrana district in 2017 has exceeded; b) Food security study is necessary every year to monitor the accessibility and level of energy and protein consumption of Jembrana residents. Keywords: Food Security, Level of Conssumption, Food Desirable Pattern
Praktik Pengobatan Gigi Sendiri yang dilakukan oleh Masyarakat Pengguna Facebook Manginar Sidabutar; Rosliani Saragih
Jurnal Skala Husada : The Journal of Health Vol 18, No 2 (2021): Jurnal Skala Husada: The Journal of Health
Publisher : Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (456.089 KB) | DOI: 10.33992/jsh:tjoh.v18i2.1838

Abstract

ABSTRACTTreating our own teeth by taking antibiotics without a doctor's prescription can trigger antibiotic resistance.The purpose of the study was to determine the characteristics of social media users who practice self-medication, dental problems and symptoms of toothache experienced, types of drugs, doses, duration, and side effects experienced, where the drugs were obtained, sources of information and reasons for self-medication. This research method is a survey using google form (web-based questionnaire). The sample in this study was a friend of the researcher on the Facebook application, amounting to 200 people. The results showed that Facebook users who did their own dental treatment were dominated by women (79.6%), under 20 years old (54.9%), students (70.8%), high school education (70.8%), and income below one million rupiah (95.5%). Symptoms of dental disease that are most often experienced are cavities/swollen gums (53.9%), the type of antibiotic commonly consumed is amoxicillin (76.1%), not knowing how many doses of amoxicillin are consumed (34.5%,). Sources of information about antibiotic drugs used were obtained from health workers at pharmacies (33.9%). In conclusion, Facebook users with low economic status and education tend to take care of their teeth by consuming antibiotics irrationally and amoxilin is a commonly used antibiotic. It is recommended that the distribution of antibiotics be strictly regulated to protect the public.ABSTRAKMengobati gigi sendiri dengan mengonsumsi antibiotik tanpa resep dokter dapat menjadi pemicu terjadinya resistensi antibiotik. Tujuan penelitian adalah untuk mengetahui karakteristik pengguna media sosial yang melakukan praktik pengobatan sendiri, masalah gigi dan gejala sakit gigi yang dialami, jenis obat, dosis, durasi, dan efek samping yang dialami, dari mana obat tersebut diperoleh, sumber informasi dan alasan untuk pengobatan sendiri. Metode penelitian ini adalah survei dengan menggunakan google form (kuesioner berbasis web). Sampel pada penelitian ini adalah teman dari peneliti di aplikasi facebook yang berjumlah 200 orang. Hasil penelitian menunjukkan bahwa pengguna Facebook yang melakukan pengobatan gigi sendiri didominasi oleh wanita (79,6%), usia di bawah 20 tahun (54,9%), pelajar (70,8%), pendidikan SMA (70,8%), pendapatan di bawah satu juta rupiah (95,5%). Gejala penyakit gigi yang paling sering dialami adalah gigi berlubang/gusi bengkak (53,9%), jenis antibiotik yang biasa dikonsumsi amoksisilin (76,1%), tidak mengetahui berapa dosis amoksisilin yang dikonsumsi (34, 5%,). Sumber informasi tentang obat antibiotik yang digunakan diperoleh dari petugas kesehatan di apotek (33,9%). Kesimpulannya, pengguna Facebook dengan status ekonomi dan pendidikan rendah cenderung merawat giginya dengan mengonsumsi antibiotik secara tidak rasional dan amoxilin adalah antibiotik yang umum digunakan. Disarankan agar pendistribusian antibiotika diatur secara ketat untuk melindungi masyarakat. 
PERAN PEMBELAJARAN MENGGUNAKAN TUTOR TEMAN SEBAYA DALAM MENINGKATKAN PERILAKU REMAJA TENTANG PENDEWASAAN USIA PERKAWINAN Putu Sri Erawati
Jurnal Skala Husada : The Journal of Health Vol 13, No 1 (2016): Jurnal Skala Husada: The Journal of Health
Publisher : Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (75.092 KB) | DOI: 10.33992/jsh:tjoh.v13i1.91

Abstract

ABSTRACT: Based on Health Research Data in 2010 about early marriage found that Indonesian women married in the age about 10 to 14 years old 0,2%, and in the age 15 to 19 years old 11,7%. Those problem can be anticipated by giving the teenagers an education about maturation age of marriage. This research aims to notice the role of peer group teaching approach to increase teenagers behavior about maturation age of marriage system in teenagers organization of Abiansemal District, Badung Regency. This designed with Experiment Pretest and Posttest Control Group Design. The variables are knowledge, attitude, and practice about maturation age in marriage, with the interval scale. The data was collected by test, which are written test. There were 17 cases had been found. Sixs  cases were intervention group and 11 cases were control group. This research fount significan deferen betwine behavior about maturation age of marriage system in teenagers organization of Abiansemal District, Badung Regency.Teenagers behavior about maturation age of marriage system in case intervention group before and after learning (p=0,046).in case control group (p =0,005). Conclution the role of peer group in teaching approach to increase teenagers behavior about maturation age of marriage system in teenagers organization of Abiansemal District, BadungKey word : Peer group, Teenagers, behavior, Maturation age of marriage
Analisis Kandungan Nitrit Pada Sosis Ayam Dan Sosis Sapi Yang Beredar Di Kota Denpasar Luh Putu Devi; I Gusti Ayu Sri Dhyanaputri; Ida Ayu Made Sri Arjani
Jurnal Skala Husada : The Journal of Health Vol 17, No 1 (2020): Jurnal Skala Husada: The Journal of Health
Publisher : Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (149.386 KB) | DOI: 10.33992/jsh:tjoh.v17i1.2058

Abstract

ABSTRACTSodium nitrite is one of the preservatives used in meat to obtain good color and prevent microbial growth. However, please note that the addition in food should not exceed the permitted limits so that it won’t give any negative affect to human’s health. Permenkes No. 722/Menkes/Per/IX/1988 about food additives limit the maximum use of the preservatine nitrite in processed meat product that is 125 mg/kg. Excessive consumption of nitrit can cause poisoning or nitrites are carcinogenic. The object of this study were 15 brands of sausages which are consist of chicken and beef sausages in Denpasar. Analysis of nitrite content in sausages using spectrophotometric method was measured at wavelength of 520 nm. Of the 15 brands of sausages samples studied, the results showed that levels of nitrite peak in chicken sausage of 12,004 mg/kg and nitrite levels as low as 0.395 mg/kg. Meanwhile the results showed that levels of nitrite peak in beef sausage of 12.086 mg/kg mg/kg and nitrite levels as low as 0,809 mg/kg. The study of 15 brands of sausages in Denpasar shows that all brands of sausages does not exceed the maximum limit the use of sodium nitrite according Permenkes 722/Menkes/Per/IX/1988.ABSTRAKNatrium nitrit merupakan salah satu bahan pengawet yang digunakan pada daging untuk memperoleh warna yang baik dan mencegah pertumbuhan mikroba. Namun perlu diperhatikan bahwa penambahan pada makanan tidak boleh melebihi batas yang diperbolehkan agar tidak memberikan dampak negatif bagi kesehatan manusia. Permenkes No. 722/Menkes/Per/IX/1988 tentang Bahan Tambahan Pangan membatasi penggunaan maksimum pengawet nitrit pada produk daging olahan yaitu 125 mg/kg. Konsumsi nitrit yang berlebihan dapat menyebabkan keracunan atau nitrit bersifat karsinogenik. Objek penelitian ini adalah 15 merek sosis yang terdiri dari sosis ayam dan sosis sapi di Denpasar. Analisis kandungan nitrit pada sosis menggunakan metode spektrofotometri diukur pada panjang gelombang 520 nm. Dari 15 merek sampel sosis yang diteliti, didapatkan hasil bahwa kadar nitrit tertinggi pada sosis ayam sebesar 12.004 mg/kg dan kadar nitrit sebesar 0,395 mg/kg. Sedangkan hasil penelitian menunjukkan bahwa kadar puncak nitrit pada sosis sapi sebesar 12,086 mg/kg mg/kg dan kadar nitrit sebesar 0,809 mg/kg. Penelitian terhadap 15 merek sosis di Denpasar menunjukkan bahwa semua merek sosis tidak melebihi batas maksimal penggunaan natrium nitrit menurut Permenkes 722/Menkes/Per/IX/1988.
ANALISIS FAKTOR YANG PALING BERHUBUNGAN DENGAN JUMLAH GIGI BERFUNGSI PADA LANSIA DI DESA MENGWI KABUPATEN BADUNG TAHUN 2015 sagung putri
Jurnal Skala Husada : The Journal of Health Vol 14, No 1 (2017): Jurnal Skala Husada: The Journal of Health
Publisher : Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (38.942 KB) | DOI: 10.33992/jsh:tjoh.v14i1.78

Abstract

ABSTRACT Generally, elderly has more experience physical setback, one of them is marked with loss teeth. The susceptible problem of dental and oral health which happened on elderly are dental caries and periodontal disease. In Indonesia, both diseases are the main cause elderly’s loss teeth.  Analysis of Factors Associated with the least amount of tooth Functioning in the Elderly in  Mengwi Badung 2015Research methods used in this research is descriptive method, and used 110 samples by Multi stage random sampling  technic. Based on data analysis bivariat dengan uji chi Square d uji regresi logistic. discussion of the research results, from 110 There was a significant correlation between the number of teeth that function in the elderly with age p = 0.000 (p 0.05). The education level of p = 0.000 (p 0.05). Economic status value of p = 0.000 (p 0.05). Concluded that the most influential factor is the level of education with a value of p = 0.000 (p 0.05) with OR 4.46,  (CI 95%  2.63 - 7.57). Suggestions addition of programs for oral health..                                                                                             Keywords: dental health, elderly, teeth function 
Efektifitas Daya Hambat Larutan Ekstrak Siwak Terhadap Pertumbuhan Bakteri Pada Plak Gigi Ratih Larasati
Jurnal Skala Husada : The Journal of Health Vol 18, No 1 (2021): Jurnal Skala Husada: The Journal of Health
Publisher : Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (432.657 KB) | DOI: 10.33992/jsh:tjoh.v18i1.1833

Abstract

ABSTRACTThe problem in the study is the accumulation of dental plaque and tongue causing decreased dental hygiene. This allows the normal flora in the mouth to become a pathogen that causes tooth decay, thus increasing the DMF-T index. The purpose of this research is to know the effectiveness of inhibitory solution of siwak extract in killing the bacteria in dental plaque. This research was purely experimental in the form of Post-test control group design. This research was conducted in the laboratory, and clinic of Dentistry Department of Poltekkes Kemenkes Surabaya. Selected respondents should fit inclusion criteria. The results of this study were not formed inhibition zone on the plate for bacterial seedling in the negative control group, whereas in the positive control group the mean diameter of the inhibit zone was 27,5 mm. Siwak extract solution concentration 50% more effective than 25% siwak extract solution. Siwak extract solution effectively inhibited bacterial growth from dental plaque (p 0.05). However, chlorhexidine 0.2% is more effective in inhibiting bacterial growth from dental plaque than siwak extract solution.ABSTRAKMasalah dalam penelitian ini adalah akumulasi plak pada gigi dan lidah yang menyebabkan buruknya kebersihan gigi. Flora normal dalam keadaan tertentu di dalam mulut dapat berubah menjadi patogen yang menyebabkan gigi karies, sehingga meningkatkan indeks DMF-T. Tujuan penelitian ini adalah mengetahui efektifitas daya hambat larutan ekstrak siwak dalam membunuh bakteri pada plak gigi. Metode penelitian ini adalah eksperimen murni dengan desain Post-test control  group. Penelitian dilakukan di laboratorium, dan klinik Jurusan Keperawatan Gigi Poltekkes Kemenkes Surabaya. Responden yang dipilih harus sesuai kriteria inklusi. Hasil penelitian, tidak terbentuk zona hambat pada lempeng bakteri kelompok kontrol negatif, sedangkan pada kelompok kontrol positif diameter rata-rata zona hambat adalah 27,5 mm. Larutan ekstrak siwak konsentrasi 50% lebih efektif daripada larutan ekstrak siwak 25%. Larutan ekstrak siwak efektif menghambat pertumbuhan bakteri dari plak gigi (p 0,05). Walaupun demikian, klorheksidin 0,2% lebih efektif menghambat pertumbuhan bakteri dari plak gigi daripada larutan ekstrak siwak. 
Perbedaan Derajat Aglutinasi Pemeriksaan Golongan Darah Metode Cell Grouping Berdasarkan Tingkat Konsentrasi Suspensi Sel 5%, 10%, dan 40% Putu Talia Jayanti; I Gusti Agung Dewi Sarihati; I Gede Sudarmanto; I Gusti Ayu Sri Dhyanaputri
Jurnal Skala Husada : The Journal of Health Vol 19, No 1 (2022): Jurnal Skala Husada: The Journal of Health
Publisher : Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (148.601 KB) | DOI: 10.33992/jsh:tjoh.v19i1.1947

Abstract

ABSTRACTExamination of ABO blood type method with cell grouping technique is to check blood cell antigen by adding anti-A, anti-B and anti-D. The strength or reaction power of agglutination produced in blood group examination is influenced by the ability of bonded antibodies or react with antigens The purpose of this study is to know the difference in the degree of agglutination of blood grouping method examination by tube test method based on the concentration level of cell supensi 5%, 10% and 40%. The research subjects used in this study were donors with blood group B, namely as many as 10 blood samples on tubes with anticoagulants EDTA used for blood screening after blood donation. On the examination of the degree of agglutination of blood group examination that has been done using cell suspension 5%, 10% and 40% obtained results where the entire examination produced a positive result 4 where found the results of one large clot with clear fluid around it. From the research that has been done, it can be concluded that there is no difference in the degree of agglutination of blood group examination using cell suspension concentrations of 5%, 10% and 40%.ABSTRAKPemeriksaan metode golongan darah ABO dengan teknik cell grouping adalah memeriksa antigen sel darah dengan cara menambahkan anti-A, anti-B dan anti-D. Kekuatan atau daya reaksi aglutinasi yang dihasilkan pada pemeriksaan golongan darah dipengaruhi oleh kemampuan dari antibodi berikatan atau bereaksi dengan antigen Tujuan penelitian ini adalah mengetahui adanya perbedaan derajat aglutinasi pemeriksaan golongan darah metode cell grouping dengan metode tube test berdasarkan tingkat konsentrasi supensi sel 5 %, 10 % dan 40 %. Subyek penelitian yang digunakan pada penelitian ini adalah pendonor dengan golongan darah B yakni sebanyak 10 sampel darah pada tabung dengan antikoagulan EDTA yang digunakan untuk screening darah setelah melakukan donor darah. Pada pemeriksaan derajat aglutinasi pemeriksaan golongan darah yang sudah dilakukan dengan menggunakan suspensi sel 5%, 10% dan 40% diperoleh hasil dimana seluruh pemeriksaan menghasilkan hasil positif 4 dimana ditemukan hasil satu gumpalan besar dengan cairan jernih disekitarnya. Dari penelitian yang sudah dilakukan dapat disimpulkan bahwa tidak ada perbedaan derajat aglutinasi pemeriksaan golongan darah menggunakan konsentrasi suspensi sel 5%, 10% dan 40%.