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Nonny Manampiring
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Jl, Kampus Unima, Tonsaru, Minahasa - North Sulawesi. Indonesia 95632
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INDONESIA
Indonesian Biodiversity Journal
Published by Universitas Negeri Manado
ISSN : 27222659     EISSN : -     DOI : -
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
International Biodiversity Journal (IBJ) is an international peer-reviewed and open access journal that publishes significant and important research from all area of biological science fields such as biodiversity, biopharmaca, DNA barcoding and molecullar biology, ecology, physiology, behavior, and biotechnology. All life forms, ranging from microbes, fungi, plants, animals, and human, including virus, are covered by International Biodiversity Journal (IBJ).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ecology, Evolution, Behavior and Systematics
Articles 49 Documents
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THE BIOACTIVE CONTENT AND ANTIOXIDANT ACTIVITY OF HONEY BEEHIVES (Apis dorsata BINGHAMI) FROM THE BOGANI NANI WARTABONE BOLAANG MONGONDOW NATIONAL PLANT Yermia S Mokosuli; Herry Sumampouw; Hendra Pratama Maliangkay; Jimmy Langi
Indonesian Biodiversity Journal Vol. 2 No. 1 (2021): April 2021
Publisher : Universitas Negeri Manado

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Abstract

Bee Honey (Apis dorsata Binghami) Is a subspecies of A. dorsata which is only found in sulawesidan island and surrounding islands and until now has not been successfully cultivated. Various studies have been conducted species of trees used for nesting places, nesting behavior, biotic conditions, physical surrounding nesting trees and nesting characteristics. This study aims to obtain secondary metabolite components and scientific data of antioxidant activity of honeybee extract (Apis dorsata Binghami) derived from Bogani Nani Wartabone Bolaang Mongondow National Park, North Sulawesi Province. The method of extraction in this research was conducted using maceration with methanol solvent, phytochemical test and antioxidant activity analysis using method (1,1-definil-2-picrilhidrazil) by measuring absorbance absorption at maximum wavelength using UV-Vis spectrophotometer. The results of the phytochemical test show that the honeycomb (Apis dorsata Binghami) contains alkaloids, flavonoids, saponins and tannins. From the analysis results showed that with the increasing concentration of extracts, the greater the antioxidant activity is marked by decreasing the value of IC50. The honeycomb (Apis dorsata Binghami) has IC50 of 212.42 ppm in comparison with vitamin C IC50 of 59.34 ppm
ANTIBACTERIAL ACTIVITY OF EXTRACTS OF LEAVES OF LEILEM (Clerodendrum minahassae Teijsm. & Binn.) ON GROWTH GRAM - POSITIVE BACTERIA Staphylococcus aureus strains ATCC 25923 Jefry Oldy Raturandang; Dintje Pendong; Masje Wurarah
Indonesian Biodiversity Journal Vol. 2 No. 1 (2021): April 2021
Publisher : Universitas Negeri Manado

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Abstract

Leaf leilem (Clerodendrum minahassae Teism. & Binn.) commonly used by the Minahasa as food and traditional medicine for treating intestinal worms, abdominal pain, and pain in the chest. Gram-positive bacteria Staphylococcus aureus ATCC 25923 strain is a type of bacteria that is found in humans and can be the cause of the emergence of the disease. This research aims to know the types of solvents are most effective and how the antibacterial activity of extracts of leaves of leilem on the growth of the bacteria Staphylococcus aureus strains ATCC 25923. The solvent used is ethanol is polar, ethyl acetate which is semi polar, and n-heksan which is non polar. Preliminary research has been conducted on konsenterasi extract with 100 ppm 200 ppm 400 ppm and 800 ppm, the antibacterial activity of the most optimal at 800 ppm so the data will be used for the analysis in this study was konsenterasi 800 ppm. Deuteronomy was done by as much as 3 times. Positive control using antibiotics clindamicyn 400 ppm and negative controls using aquades and 10% DMSO. Isolation of bacteria using scratch in zig-zag and to test the antibacterial activity of using diffusion well in order. Results of the study showed that the percentage yield of solvent is ethanol that is 1,2329% in maceration 1:4. Drag the diameter of most bacteria are grown on ethyl acetate extract polar spring with a grade average of 12.6 mm. Based on the analysis of OneWay ANOVA showed different results for real then followed by Tukey test, to see that these three types of different extract significantly in inhibiting the growth of gram positive bacteria Staphylococcus aureus strains ATCC 25923. In conclusion, namely the first ethanol is a solvent that is most effective in dissolving the leaves leilem, both of the antibacterial activity in sequence from the most minor to the most massive is the n-heksan (non-polar), ethanol (polar), and ethyl acetate (semi polar). The third conclusion in the optimum konsenterasi 800 ppm, extract n-heksan n-heksan (non-polar), ethanol (polar), and ethyl acetate (semi polar) showed antibacterial activity significantly different.
ANTIOXIDANT ACTIVITY TEST AND EFFECT OF ETHANOL EXTRACT OF NUSA INDA PUTIH (Mussaenda pubescens) AGAINST CREATININE, URIC ACID AND UREUM LEVELS IN WHITE RATS (Rattus novergicus) Rolef rumondor; Rino Komalig
Indonesian Biodiversity Journal Vol. 2 No. 1 (2021): April 2021
Publisher : Universitas Negeri Manado

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Abstract

The purpose of this study was to examine the antioxidant activity and the effect of the extract of Nusa Indah Putih (Mussaenda pubescens) on creatinine, uric acid and urea levels in white rats (Rattus novergicus). The study design, the treatment group was divided into 5 groups. The control group (P0), the ethylene glycol group (P1), the kidney drug treatment group (P2), the Nusa Indah Putih ethanol extract group at a dose of 100 mg/kgBW (P3), and the Nusa Indah Putih ethanol extract group at a dose of 200 mg/kgBW (P4 ). Nusa Indah Putih extract has strong antioxidant activity with IC50 value of 78.27 ppm and AAI value of 1.277. Nusa Indah Putih extract was effective in lowering creatinine, uric acid and urea levels in experimental animals.
ANTILITHIASIS EFFECTIVENESS TEST OF ETHANOL EXTRACT OF ALANG-ALANG (Imperata cylindrica (L.) Beauv) ON WHITE RATS (Rattus novergicus) Stenly Komansilan; Rolef Rumondor
Indonesian Biodiversity Journal Vol. 2 No. 1 (2021): April 2021
Publisher : Universitas Negeri Manado

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Abstract

The purpose of this study was to determine the antilithiation effectiveness of alang-alang extract in white rats by looking at the histology of the rat kidney. The study design, the treatment group was divided into 5 groups. Control group (P0), ethylene glycol group (P1), Hydrochlorothiazide treatment group (P2), alang-alang extract group at a dose of 100 mg/kgBW (P3), and alang-lang extract group at a dose of 200 mg/kgBW (P4). The results showed that alang-alang extract had activity in repairing the kidneys of white rats with urolithiasis. And the alang-alang extract dose of 200 mg/kgBW has better effectiveness.
ANTAGONIST, ANTIBACTERIAL ACTIVITY OF Stapylocossus aureus AND ISOLATE OF ORAL BACTERIA FROM THE ENDOGENOUS FUNGUS Apis dorsata BINGHAMI NEST Dina Rombot; Ellen Adil; Mario Walean; Virjinia Bastian
Indonesian Biodiversity Journal Vol. 2 No. 2 (2021): Agustus 2021
Publisher : Universitas Negeri Manado

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Apis dorsata Binghami is a honey bee endemic to Indonesia, living naturally in the forests of Sulawesi. This study aimed to obtain isolates and characteristics of endogenous fungi antibiotic activity from Apis dorsata Binghami nest. The study consisted of isolation of fungi from beehives using potato dextro agar, pure culture of fungi, antagonist test and antibiotic test using disc diffusion method. Antibiotic test was performed on oral bacteria and Staphylococcus aureus. The results obtained six fungal isolates from Apis dorsata Binghami's nest, namely isolates FAB1, FAB2, FAB3, FAB4, FAB5 and FAB6. The results of the antagonist test showed that the isolates FAB1, FAB2, FAB3, FAB4 and FAB5 were antagonistic. The results of the antibacterial test showed that all isolates produced an average bacterial growth inhibition of 17-18 mm on Staphylococcus aureus and isolates of oral bacteria. The inhibition zone lasts up to 3 x 24 hours so that the activity of the bacteria is bactericidal. From the results of this study, it can be concluded that the endogenous fungus Apis dorsata Binghami is a potential source of antibacterial bioactives.
BIOAKTIVASI LARVASIDA NYAMUK Aedes sp. DARIiEKSTRAK DAUNiECENGiGONDOK (Eichhornia crassipes (Mart.) Solms) DARI DANAUiTONDANO Jonathan Polandos; Revolson Alexius Mege; Yermia Mokosuli
Indonesian Biodiversity Journal Vol. 3 No. 1 (2022): April 2022
Publisher : Universitas Negeri Manado

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Nyamuk Aedes sp. adalah vektor utama penyakit demam berdarah dengue yang mempunyai kiprah besar pada terhadap penyebaran penyakit DBD di Indonesia. Pengendalian penyebaran vektor nyamuk dibutuhkan agar dapat menurunkan populasi vektor nyamuk Aedes sp. sehingga dapat menurukan masalah penyebaran DBD. Salah satu pengendalian vektor penyakit DBD yaitu mengunakan larvasida yang berasal dari tumbuhan. Ekstrak alkohol daun eceng gondok (Eichhornia crassipes (Mart.) Solms) diduga memiliki pengaruh larvasida Aedes sp., sebab mempunyai kandunan flavonoid, saponin dan tanin yang mengganggu perkembanan larva nyamuk Aedes sp. Tujuan dari penelitian ini untuk mengetahui efetivitas ekstrak (crude extract) alkohol 70% daun eceng gondok (Eichhornia crassipes (Mart.) Solms) terhadap mortalitas larva nyamuk Aedes sp. Penelitian eksperimental dilakukan dengan subyek dari penelitian yaitu 150 larva nyamuk Aedes sp. instar 3. Subyek penelitian dibagi menjadi lima grup pelakuan, konsentrasi kandungan yang digunakan di penelitian adalah 1000 ppm, 1500ppm, 2000 ppm, 2500ppm dan untuk kontrol positif adalah abate 1%. Pengamatan dilakukan 1x24 jam di ke 24 jam setelah perlakuan, perlakuan dilakukan replikasi sebanyak 3 kali. Data dianlisis serta dilakukan penghitungan nilai Lethal consentration mengunakan analisis probit. Berdasarkan analisis probit tidak ditemukan nilai dari LC50 di seluruh konsentrasi. Ekstrak (crude extract) alkohol 70% daun eceng gondok (Eichhornia crassipes (Mart.) Solms) tidak mempunyai efek larvasida nyamuk Aedes sp. Kesimpulan ekstrak (crude extract) alkohol 70% Daun eceng gondok (Eichhornia crassipes (Mart.) Solms) tidak mampu membunuh nyamuk vektor penyakit DBD nyamuk Aedes sp. di seluruh tingkat konsentrasi sesudah dilakukan pengamatan selama 24 jam.
POTENTIAL ANTIBACTERIAL EXTRACTS OF MANGROVE GENUS AVICENIA ROOT AND LEAVES FROM KOMBI BEACH MINAHASA Masje Wurarah; Devitha Kalitouw; Raflen Aril Gerungan
Indonesian Biodiversity Journal Vol. 2 No. 3 (2021): Desember 2021
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This study aimed to obtain the phytochemical content of the crude extract of leaves and roots of Avicenia spp from Kombi Beach Minahasa. Obtaining data on the activity of inhibiting the growth of infective microbes on external wounds of crude extract of leaves and roots of Avicenia spp from Kombi Beach, Minahasa. To determine the concentration of minimum growth inhibition of crude extract of leaves and roots of Avicenia spp from Kombi Beach, Minahasa. Knowing the crude extract (crude extract) leaves and roots of Avicenia spp originating from the Minahasa Kombi Beach is bactericidal or bacteriostatic. This research consisted of extraction, phytochemical analysis and antibacterial activity test. The results showed that the dominant secondary metabolites in the root and leaf extracts were alkaloids, flavonoids, saponins and tannins. The chloroform extract inhibited the growth of bacteria in infected wounds more than the ethanol and n-hexane extracts, at concentrations of 15 and 30 ppm for the test solution of ethanol extract with an inhibitory diameter of < 7 mm and a concentration of 50 ppm with an inhibitory diameter of > 10 mm indicating that the high bioactive activity antibacterial.
ANTIBACTERIAL ACTIVITY OF CHLOROFORM EXTRACT AND RHIZOPHORA SPP LEAVES METHANOL EXTRACT ON MOUTH BACTERIA Carolin Manuahe; Herry Sumampouw; Friska Montolalu
Indonesian Biodiversity Journal Vol. 2 No. 3 (2021): Desember 2021
Publisher : Universitas Negeri Manado

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The purpose of this study was to obtain the phytochemical profile of the methanol and chloroform extracts of the leaves of Rhizophora apiculata and to find the antibacterial activity of the methanol and chloroform extracts against human oral bacteria. This research was carried out in 3 stages, namely Rhizopora spp. Leaf Sampling, Extraction and Analysis of Secondary Metabolite Content and Antibacterial Activity Test using the agar diffusion method. Based on the results of the phytochemical screening examination of the leaf extract of Rhizopora spp., the results showed that it was rich in chemical compounds of alkaloids, flavonoids, tannins, and saponins in both methanol extract and chloroform extract. The results of the antibacterial activity test of methanol extract obtained that the smallest concentration in bacterial isolate bacteria 2 was 30 mg/ml, while the smallest concentration in bacterial isolate 1 was 50 mg/ml. The results of the antibacterial activity test of the chloroform extract obtained that the smallest concentration in the bacterial isolate bacteria 2 was 20 mg/ml while the smallest concentration in the bacterial isolate 1 was 30 mg/ml. Both methanol extract and chloroform extract had better activity on gram-positive bacteria.
BIOLOGY OF YELLOW SELAR FISH (Caranx leptolepis) AND ICE PRESERVED PROTEIN, LIPID AND CARBOHYDRATE CONTENT Jeffry Raturandang; Femmy Rogahang; Fandi Tuju
Indonesian Biodiversity Journal Vol. 2 No. 3 (2021): Desember 2021
Publisher : Universitas Negeri Manado

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Yellow Selar fish (Caranx leptolepis) as pelagic fish are mostly caught by fishermen in North Sulawesi. However, from observations and preliminary research, fishermen often do not pay attention to fish sustainability because even small fish are caught. This is motivated by the lack of knowledge of fishermen and the lack of research on yellow selar fish in Sulawesi. Research has been carried out with the aim of obtaining the Biological Characteristics of Yellow Selar fish caught by fishermen in North Sulawesi. Obtaining the biochemical characteristics of selar kuning fish (macronutrient content). The results that have been achieved: The average total length of Caranx leptolepis without distinguishing males and females is 180 mm and weighs 90 gr. Fish gonad maturity index is between 1.02 to 1.90. From the stomach contents that we observed, the types of food eaten included small fish and shrimp. A total of 96% of small fish and 4% of shrimp were found in the stomach contents of the slugs that were analyzed. Protein and Fat content of Selar fish decreased with the length of storage time using ice. However, the carbohydrate content remained relatively unchanged. The best storage time for tude on ice is 48 hours. More than 48 hours there is a very significant decrease in protein and fat content.
PHYTOCHEMICAL ANALYSIS OF BITUNG TREE SEEDS (Baringtonia asiatica L.) Hendra Pratama Maliangkay; Rolef Rumondor; Mario Walean
Indonesian Biodiversity Journal Vol. 2 No. 3 (2021): Desember 2021
Publisher : Universitas Negeri Manado

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This study aims to obtain data on the phytochemical content of the methanol extract of Baringtonia asiatica. Obtain data on the phytochemical content of the B. asiatica clorform extract. This research was carried out in the Biology laboratory and Chemistry Laboratory, State University of Manado. The study consisted of extraction and phytochemical analysis using the Harborne method, the results showed that from the extraction process the highest yield was obtained in extracts with n-hexane solvent, then chloroform and methanol. The results of phytochemical analysis show that saponins are the phytochemical group with the strongest intensity found compared to other phytochemical groups. Furthermore, flavonoids were found in high intensity also in the analyzed samples.

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