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Nonny Manampiring
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nonny@unima.ac.id
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+6285298111186
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nonny@unima.ac.id
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Jl, Kampus Unima, Tonsaru, Minahasa - North Sulawesi. Indonesia 95632
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Kab. minahasa,
Sulawesi utara
INDONESIA
Indonesian Biodiversity Journal
Published by Universitas Negeri Manado
ISSN : 27222659     EISSN : -     DOI : -
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
International Biodiversity Journal (IBJ) is an international peer-reviewed and open access journal that publishes significant and important research from all area of biological science fields such as biodiversity, biopharmaca, DNA barcoding and molecullar biology, ecology, physiology, behavior, and biotechnology. All life forms, ranging from microbes, fungi, plants, animals, and human, including virus, are covered by International Biodiversity Journal (IBJ).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ecology, Evolution, Behavior and Systematics
Articles 49 Documents
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MORPHOLOGY OF Patchouli OIL MICROCACULES (Pogostemon cablin Benth) WHICH IS ENKAPSULATED USING CHITOSAN Kleriyani Mayan; Arrijani; Emma Moko
Indonesian Biodiversity Journal Vol. 1 No. 2 (2020): Agustus 2020
Publisher : Universitas Negeri Manado

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Abstract

Patchouli oil (Pogostemon calbin Benth) has economic benefits which are widely used as raw material for the pharmaceutical, perfume and food industries. Although widely used in various fields, patchouli oil or essential oils are generally young to evaporate, are susceptible to high temperatures and humidity, so we need a way / solution to overcome this, one way is encapsulation. Encapsulation is a technique to protect the core material which was originally liquid from becoming a solid / powder form and can protect the core material from losing its taste and aroma. Coating is a material that can mix with the core material, is not reactive to the core material and can form a layer around the core material. Chitosan is a good coating for essential oil encapsulation. This study aims to determine the particle size and microcapsule characteristics of patchouli oil (P.calbin Benth) using chitosan coating. The microcapsules were made using a freeze dryer with the freeze drying method. The dried samples were analyzed for particle size, morphological shape and content using SEM (Scanning Electron Microscopy). The results showed that the samples with a coating material composition of 1:2 were completely dry freeze drying. The average particle size <3.5 µm with a magnification of 10,000x with round morphology and various shapes. Analysis of the chemical content in the sample showed the similarity of the main compounds in patchouli essential oil, namely α-guaiene, azulene, α/β/ γ-patchoulene, seychellene, and α-himachalene and there was the addition of carbon (C) 76.07% and oxygen. (O) 16.46% originating from coating material
Application Of Apis dorsata Binghami Bee Nest Extract On The Effects Of Human Blood Lipid Reduction Dina Munawaroh; Yermia S Mokosuli; Anita C. C. Tengker
Indonesian Biodiversity Journal Vol. 2 No. 2 (2021): Agustus 2021
Publisher : Universitas Negeri Manado

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Abstract

Honey, royal jelly, bee pollen, bee wax, propolis, bee glue, and bee venom/ apitoxin are among the items produced by honey bees. Honey, pollen, bees, eggs, larvae, and pupae are all stored in the A. dorsata Binghami beehive. Secondary metabolites and bioactive phenolic compounds (phenols, phenolic acids, esters, flavors, dihydroflavones, flavanols, chalcones, and phenolic glycerides), essential oils (mono and sesquiterpenes), polysaccharides, amino acids, amines, lactones, quinones, steroids, and vitamins) can all be found in honeycomb. The goal of this study is to see how A. dorsata Binghami honeycomb tea affects blood lipid levels in humans after 14 days of consumption in a small group of male and female volunteers who were given a 2 gram tea bag extract of A. dorsata Binghami honeycomb simplicia to drink once a day to see how it affects lipid levels (total cholesterol, LDL, HDL, and TAG) in human blood. Total cholesterol (140.56 mg/dL), triglycerides (140.93 mg/dL), LDL (85.92 mg/dL), and HDL (21.49 mg/dL) were all found to be high in humans. As a result, "The Use of A. dorsata Binghami's Honeycomb Extract on the Effect of Reducing Human Blood Lipids" can lower lipid levels.
Effectiveness Test of Tome-tome Fruit Ethanol Extract (Flacourtia inermis,Roxb) against Antidiabetes in White Rats (Rattus norvegicus) Induced by Alloxan Fricilia Leony Mey Jacob; Meity Neltje Tanor; Nonny Manampiring
Indonesian Biodiversity Journal Vol. 2 No. 2 (2021): Agustus 2021
Publisher : Universitas Negeri Manado

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Abstract

Diabetes mellitus is a chronic metabolic syndrome disorder of carbohydrates, lipids, and fats caused by insulin deficiency in the body causing blood glucose above the normal threshold (hyperglycemia). Tome-tome (Flacourtia inermis, Roxb) contains anthocyanins which have the ability to lower blood sugar levels. This study aimed to determine the effectiveness of the ethanolic extract of tome-tome fruit (Flacourtia inermis, Roxb) and to see what dose and day treatment was most effective against diabetes in white rats (Rattus norvegicus) induced by alloxan. The place of research was carried out at the Pharmacy Laboratory of Trinita University, Manado with a research time of 3 months. The test animals used were 25 white rats (Rattus norvegicus) divided into 5 treatment groups, namely normal control, negative control, positive control (glibenclamide) and ethanol extract of tome-tome fruit with doses of 150 mg/kgBW and 300 mg/kgBW. Blood glucose measurements were carried out on day 1, day 7, and day 14. The results showed that the effectiveness of tome-tome fruit ethanol extract against alloxan-induced diabetic rats decreased blood glucose levels. A dose of 150 mg/kgBW ethanol extract of tome-tome fruit was more effective in reducing blood glucose levels compared to a dose of 300 mg/kgBW rats.
IDENTIFICATION GASTROINTESTINAL PARASITES IN THE SMALL INTESTINE, LARGE INTESTINE, AND FECES OF LOCAL PIG FROM NORTH SULAWESI Kezia Andries; Revolson Alexius Mege; Nonny Manampiring
Indonesian Biodiversity Journal Vol. 2 No. 2 (2021): Agustus 2021
Publisher : Universitas Negeri Manado

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Abstract

The purpose of this study was to identify gastrointestinal parasites in the small intestine, large intestine, and feces of local pigs from North Sulawesi. Sampling in this study was carried out in Termaal Village, Likupang Barat District, North Minahasa Regency and in Poopoh Village, Tombariri District, Minahasa Regency. Observations and identification of parasites were carried out at the Biology Laboratory of the Faculty of Mathematics and Natural Sciences, UNIMA. Samples of small intestine fluid, large intestine, and pig feces were taken totaling 9 samples. The sample was examined by the Floating Test method, and if parasites were identified, a literature study was carried out with reference to several sources and previous research books to determine the type of parasite species found. The results of this study indicate that all samples observed and identified were positive for the presence of intestinal parasitic worms. The parasitic worms found were Ascaris lumbricoides, Strongyloides ransomi, Trichuris suis, Taenia solium, and Fasciolopsis buski.
DESCRIPTION OF NUTRITIONAL STATUS IN ELEMENTARY SCHOOL STUDENTS: A CASE STUDY OF SD GMIM 65 WINURI, EAST LIKUPANG DISTRICT, NORTH MINAHASA REGENCY Revolson Alexius Mege; Maria Helena; Sintia Djafar; Megawati Weken; Maiko Polandos; Nonny Manampiring
Indonesian Biodiversity Journal Vol. 2 No. 2 (2021): Agustus 2021
Publisher : Universitas Negeri Manado

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Abstract

Nutrition plays an important role in the human life cycle from fetus to the elderly. Malnutrition in infants and toddlers can cause growth and development disorders that continue into adulthood if not treated early. Good quality of children can be achieved by ensuring that the process of child development is also good. Growth is quantitative, such as height, weight, and head circumference, and development is the gradual change and improvement of abilities, such as movement, sensory, language, and social abilities. This research was conducted at SD GMIM 65 Winuri. The sample of this study were all elementary school children at SD GMIM 65 Winuri. The results showed that the most common age characteristics of the respondents were 7 years old by 20%, the sex characteristics were mostly male by 52%. Nutritional status based on body mass index (BMI) according to age, respondents have less nutritional status by 18%, normal nutritional status by 50%, overweight status by 14%, nutritional status of obesity I by 8%, and nutritional status of obesity II by 10%.
The Effect Of Combination Of Technology Of Planting And Control Of Weeds On The Dominant Value Of Weeds And Rice Productivity Yuriko Kasim
Indonesian Biodiversity Journal Vol. 3 No. 1 (2022): April 2022
Publisher : Universitas Negeri Manado

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Abstract

The problem currently arises in lowland rice cultivation is the presence of nuisance plants, namely weeds which can reduce crop yields by up to 90%. Therefore, it is necessary to find a way out by testing several weed control technologies that farmers can use. The objectives of the study were (1) to determine the effect of technology for planting methods, namely Tabela in arrays, evenly spaced tables, and evenly distributed tables, on the growth and production of lowland rice, (2) To determine the effect of weed control technology using herbicides with active ingredients metsulfuron-methyl and 2,4 -D on the growth and production of lowland rice and (3) To determine the effect of the Combination of planting technology and weed control technology on the growth and production of lowland rice. The experiment was carried out using a Randomized Block Design (RAK) as the environmental design. The treatment design was a split-plot design: Main Plot, Planting Method Technology (T): T1 = Array Tables, T2 = Distance Tables, T3 = Tables Spread Evenly. Sub-plots, Weed Control Technology (G): G1 = Herbicide with active ingredient mtsulfuron methyl, G2 = Herbicide with active ingredient 2,4-D, G3 = Combination of mtsulfuron methyl and 2,4-D. Thus there were nine treatments, each treatment was repeated three times, so there were 27 experimental units. One experimental plot measuring 4 x 6 m. The placement of all treatments in the experimental plot was done randomly. The dominant weed species observed were Paspalum disticum, Fibristylis litoralis, Marsilea crenata, Cyperus iria and Echinochloa colonum. The dominant weed species in all treatment combinations observed at 21 DAP were Paspalum distichum, Echinochloa crusgalli, Marsilea crenata, Lersia hexandra and Fimbristylis litoralis. There was no difference in rice production in all treatment combinations. The difference in production only occurred in the average planting method, where the highest production occurred in the T1 treatment.
ANALYSIS OF THE EFFECT OF PGPR FERTILIZER USE ON GROWTH ACCELERATION OF CHERRY TOMATO (Solanum lycopersicum var. cerasiforme) Genade Kojongian; Orbanus Naharia; Helen Lawalata
Indonesian Biodiversity Journal Vol. 3 No. 1 (2022): April 2022
Publisher : Universitas Negeri Manado

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Abstract

Cherry tomatoes are a type or commodity of horticulture that has a very relatively high economic value compared to ordinary tomatoes. Cherry tomato plants (Solanum lycopersicum var,. cerasiforme. It is one of the local plants that is neglected and not used and is often considered as a wild plant. This study aims to determine the effect of PGPR fertilizer application on the growth of cherry tomato plants. This research was carried out in the urban village). Rerewokan, West Tondano sub-district starting from August to November 2021. The method used is a quantitative method with the type of experimental research in the form of a Randomized Block Design (RAK) with five types of treatment, each repeated four times. P1 = without using fertilizer, P2 = 100ml, P3 = 200ml, P4 = 300ml, P5 = 400ml, so there were 20 treatment combinations, and the data obtained were then analyzed using analysis of variance (ANOVA). From the results of this study, it showed that good plant height growth was found in treatment P4 with the application of a mixture of PGPR fertilizer concentration of 300ml / 2 liters of water, because there was the most effective growth, while the growth in the number of leaves and the number of fruits was very good in treatment P2 with the addition of a mixture. PGPR fertilizer concentration 100ml / 2 liters of water.
ANALYSIS OF THE STATUS OF THE GIRIAN WATERSHED IN BITUNG CITY DUE TO HOUSEHOLD WASTE POLLUTION Rivaldo Tompoh; Arrijani; Nova Ogi
Indonesian Biodiversity Journal Vol. 3 No. 1 (2022): April 2022
Publisher : Universitas Negeri Manado

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Abstract

The Girian watershed with a length of 17.5 km is the longest river in Bitung City which flows through a densely populated area as well as various industries such as smoked fish, tofu and tempeh, animal husbandry, agriculture, and industrial fish factories whose waste goes directly to the Girian watershed. The great deal of population activities can have an impact on water quality and the Girian watershed ecosystem. This study aims to analyse the water quality of the Girian watershed caused by domestic waste. This study uses a quantitative descriptive approach and the method used in this study is the Indonesian National Standard (SNI) according to the water quality parameters that have been determined. The results of the analysis of water quality upstream to downstream show that there is a decrease in water quality. Upstream is classified as good when compared to the parameters that have been determined. Densely populated areas begin to experience a decrease in water quality, namely with DO and Oil and Fat that exceeds the predetermined quality standard. As well as estuaries that have concentrations of TSS, NH3-N, Oils and Fats, COD, and BOD that exceed the specified quality standards. The results of the calculation of the pollution index show that station point 1 upstream is classified as good in 4 groups, station point 2 in densely populated areas shows lightly polluted results in group 2, and The Station 3 estuary shows moderate pollution results in group 3
IDENTIFIKASI MOLEKULER BAKTERI PUPUK ORGANIK SISTIM BIOPORI MENGGUNAKAN GEN 16S rRNA Feren Pangerapan; Suddin Simanjuntak; Decky Kamagi
Indonesian Biodiversity Journal Vol. 3 No. 1 (2022): April 2022
Publisher : Universitas Negeri Manado

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Abstract

Bakteri dari pupuk organik sistim biopori masih perlu ditentukan kedudukan dalam klarifikasi bakteri. Untuk itu perlu dilakukan identfikasi pada tingkat spesies menggunaan analisis sekuen gen 16S rRNA. DNA bakteri dari pupuk organik sistim biopori di ekstrak untuk di identifikasi secara molekuler menggunakan gen 16S rRNA. Isolat yang diperoleh berasal dari sampel pupuk organik sistim biopori. Bakteri di isolasi pada media NA dengan metode tabur dan di inkubasi pada suhu kamar selama 2x24 jam. Isolate yang tumbuh pada media NA diekstrasi menggunakan Presto Mini gDNA Bacteria KIT. Amplifikasi gen 16S rRNA dengan metode PCR, visualisasi amplikon gen 16S rRNA dengan metode elektroforesis, Amplifikasi DNA target dilakukan dengan menggunakan primer 16sA dan 16sB2. Amplicon-amplikon dielektroforesis menggunakan gel agarose 1,6 dengan DNA Ladder 500-1500bp. Sekuensing menggunakan jasa sekuensing First Base Singapura. Hasil analisis penyelarasan menggunakan program BLAST (www.ncbi.nih.gov.com) 16S rRNA urutan isolat bakteri pupuk organik bp4 sistim biopori menunjukkan kemiripan 98% dengan Bacillus cereus strain MH19 (nomor tambahan CP039269.1). Hasil rekonstruksi filogeni dengan Neighbour Joining, urutan gen 16S Rrna isolate bp4 menunjukkan hubungan terdekat dengan Bacillus cereus strain MH19 (nomor tambahan CP039269.1).
IDENTIFICATION OF ETHNOMEDICAL MEDICAL PLANTS FOR DEGENERATIVE DISEASES IN TARERAN DISTRICT, SELATAN MINAHASA REGENCY, NORTH SULAWESI, INDONESIA Ferny Tumbel; Friska Montolalu; Devitha Kalitouw
Indonesian Biodiversity Journal Vol. 1 No. 3 (2020): Desember 2020
Publisher : Universitas Negeri Manado

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Abstract

This study aims to: Obtain distribution data and types of medicinal plants in Tareran District, South Minahasa. Obtain data on the use of medicinal plants for degenerative diseases in Tareran District, South Minahasa. Plant samples can be in the form of leaves, stems and roots or whole plants obtained in Tareran District, South Minahasa Regency. Samples of medicinal plants are devoted to medicinal plants that are used for degenerative diseases. The samples used were wet samples and dry samples (10% moisture content) and wet samples. Materials used include ethanol, alcohol, plant specimen paper, electric oven, etc. Inventory of medicinal plants using plant determination books and writing instruments, cameras and specimen boxes. Data on the use/utilization of medicinal plants using a questionnaire. The results showed that in Tareran District there are many types of plants used as medicinal plants for degenerative diseases, including: Cinnamon (hypertension), Noni (Diabetes), Temulawak (hyperlipidemia). The community has not cultivated medicinal plants intensively. Public knowledge of the scientific efficacy of medicinal plants is still very little so that it affects the utilization of these medicinal plants. In terms of the use of medicinal plants, the economically weak community uses medicinal plants more than the middle to upper economic community. In fact, there are more doubts about the efficacy of medicinal plants in the middle and upper economic community. The use of medicinal plants in the middle to upper economic community is only carried out by the elderly.

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