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BAKTERI ASAM LAKTAT BERMANFAAT DALAM KEFIR DAN PERANNYA DALAM MENINGKATKAN KESEHATAN SALURAN PENCERNAAN I Putu Aditya Prayoga; Yan Ramona; Ida Bagus Made Suaskara
SIMBIOSIS Vol 9 No 2 (2021)
Publisher : Jurusan Biologi Universitas Udayana

ABSTRAK Kefir merupakan produk fermentasi susu yang melibatkan berbagai macam bakteri asam laktat (BAL) dalam fermentasinya. Bakteri asam laktat di dalam kefir telah banyak dilaporkan memberi manfaat menyehatkan bagi saluran pencernaan manusia yang mengonsumsinya, dengan cara mengendalikan atau mengatur populasi bakteri patogen. Telaah ilmiah ini menjabarkan berbagai BAL dan perannya dalam proses pembuatan kefir, mekanisme yang dilakukan BAL dalam mengendalikan populasi patogen dalam saluran pencernaan, serta efek fungsional BAL terhadap produk kefir. Dalam telaah literature ini diperoleh informasi bahwa Lactobacillus kefiranofaciens, L. kefiri, L. casei, L. delbrueckii dan L. brevis merupakan kelompok BAL yang paling umum terlibat dalam pembuatan kefir. Spesies–spesies BAL tersebut berperan dalam mengendalikan populasi mikroba saluran pencernaan melalui berbagai mekanisme, seperti produksi asam laktat, etanol, diasetil, hidrogen peroksida, reuterin atau bakteriosin. Efek fungsional yang dapat dilakukan oleh BAL setelah berada di dalam saluran pencernaan antara lain mencegah terjadinya infeksi bakteri patogen, menurunkan kadar kolesterol darah, meningkatkan sistem imun, dan sebagai antioksidan. Hal ini mengindikasikan bahwa BAL yang diisolasi dari kefir berpeluang dikembangkan sebagai probiotik potensial dimasa yang akan datang. Kata Kunci: BAL, kefir, sistem pencernaan manusia

UJI DAYA HAMBAT ASAP CAIR TEMPURUNG KELAPA (Cocos nucifera L.) TERHADAP PERTUMBUHAN Escherichia coli SECARA In Vitro Rachel Daniella Dinda Maria Lumban Tobing; Made Ria Defiani; Ni Made Susun Parwanayoni
SIMBIOSIS Vol 9 No 2 (2021)
Publisher : Jurusan Biologi Universitas Udayana

Meat contaminated with bacteria has the potential to cause dangerous diseases when consumed by humans. One of the germs, especially bacteria that contaminate raw meat or meat with an incomplete maturation process is Escherichia coli. Therefore, E. coli needs to be minimized by inhibiting its growth. One natural way to inhibit the growth of E. coli is to test for antibacterials using liquid smoke. Liquid smoke can be obtained through the pyrolysis process from various biomass containing cellulose, hemicellulose, and lignin such as coconut shell (Cocos nucifera L.). Liquid smoke contains phenolic and acid compounds which are antimicrobial and antioxidant. This research uses the diffusion well method. Data analysis used Completely Randomized Design (CRD) with variance test (ANOVA). If the data had a significant difference at the 5% test level (P?5), the Duncan test was continued. Coconut shell liquid smoke is able to inhibit the growth of E. coli which is indicated by the formation of an inhibition zone. The concentration of coconut shell liquid smoke which effectively inhibited the growth of E. coli was a concentration of 50% with an inhibition zone diameter of 16.66 mm and MIC at a concentration of 10% with an inhibition zone diameter of 9.33 mm. Phytochemical test results of coconut shell liquid smoke contain positive phenolic compounds, flavonoids, triterpenoids, and saponins as well as phenol content of 2.403% and saponin content of 5.50%.

DNA HASIL EKSTRAKSI DARI BERCAK SPERMA PADA KAIN KATUN DAN POLIESTER YANG DISIMPAN HINGGA 40 HARI I Gede Yeyen Suharta; I Ketut Junitha; A.A.S.A. Sukmaningsih
SIMBIOSIS Vol 9 No 2 (2021)
Publisher : Jurusan Biologi Universitas Udayana

DNA is an important evidence in forensic-based investigation which is useful in the process of solving criminal cases. Rape is an act of crime which could leave evidence such as sperm stain on fabric. The said stain can be processed to obtain the perpetrator’s DNA and uncover their identity. This study aims to determine whether DNA from sperm stains on cotton and polyester fabrics which had been stored for 0, 20, and 40 days could be extracted and to determine the quality and quantity of the extracted DNA. This study conducted from September to December 2020 at the Serology and Molecular Forensics of UPT Laboratory and the Integrated Biomedical Laboratory of Udayana University. The research method consisted of sample preparation, DNA extraction using Chelex 100 resin, and DNA quantification and qualification using NanoDrop. Data from DNA quantity are analyzed using two-way ANOVA and chi-squared test method. Results of this study shows that DNA can be extracted from sperm stains on cotton and polyester fabrics which are stored for up to 40 days. The decrease of DNA quality and quantity in all samples directly proportional to the increase of the storage time treatment. The decrease of DNA quantity on cotton fabric occurs by 80.42% (fresh-0 day), 12.02% (0 day-20 days), and 0.78% (20 days-40 days). Decrease of DNA quantity on polyester fabric occurs by 62.16% (fresh-0 day), 11.40% (0 day-20 days), and 8.47% (20 days-40 days). Statistical analysis on the quantity of DNA showed that the rate of reduction in DNA concentration was influenced by the type of cloth used.

AKTIVITAS HARIAN DAN FREKUENSI BEGGING BEHAVIOUR DARI ELANG BONDOL (Haliastur indus) SITAAN DI PUSAT PENYELAMATAN SATWA, TABANAN, BALI Gde Oka Widiyavedanta; Luh Putu Eswaryanti Kusuma Yuni; I Ketut Ginantra
SIMBIOSIS Vol 9 No 2 (2021)
Publisher : Jurusan Biologi Universitas Udayana

Brahminy kite (Haliastur indus) is one of bird of prey species that occupy position as top predator. Many brahminy kite was captured and was kept as pet which caused those birds lose their ability to hunt as a result of being caged for too long and for not to be trained to hunt. In Bali Wildlife Rescue Centre, the confiscated brahminy kite showed begging behavior in which the birds “whine” every time a human approach. This kind of behaviour affect their readiness to be released in the wild. The purpose of this study was to determine the daily activity and the frequency of begging behaviour of confiscated brahminy kite in Bali Wildlife Rescue Centre. This study was conducted in January 2020. This study found that the brahminy kite at the Bali Wildlife Rescue Centre spent the most of their time for perching. The second highest activity was preening, whereas the least activities were feeding and moving. The highest percentage to beg in brahminy kite was recorded in the morning, whereas the lowest percentage was in the midday. The results of this study indicate that the confiscated brahminy kite at the Bali Wildlife Rescue Centre still needs more time to be rehabilitated prior released ack into the wild.

PENGARUH PENCUCIAN DETERJEN HASIL EKSTRASI DNA SPERMATOZOA PADA KAIN KATUN Christian Subagya Gunardi; I Ketut Junitha; Inna Narayani
SIMBIOSIS Vol 9 No 2 (2021)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2021.v09.i02.p01

The presence of spermatozoa DNA is reliably counted as a solid evidence when dealing with rape cases. However, police department often finds difficulties to obtain it, mainly because it has been destroyed by the perpetrator. Such act can be done by washing semen stains on the clothing with detergent. The purpose of this research is to analyze the impact of detergent as a washing property towards spermatozoa DNA extract on a cotton fabric. Observed variables in this study are quantity and quality of spermatozoa DNA extract from semen stain on cotton fabric. Samples are divided based on two separate treatments; detergent-washed and unwashed. Furthermore, samples are stored with different storage time. This research applied two methods; DNA extraction using kit (Roche) and DNA quantification using spectrophotometer Nanodrop. The research was held at Biomedic Laboratory, Faculty of Medicine, Udayana University, Denpasar with ethical clearance. Seminal plasma sampel was obtained from a donor with informed consent. This research showed that seminal stain washing on cotton fabric doesn't remove the DNA until two-week-storage time.

STRUKTUR KOMUNITAS PLANKTON DI DANAU TAMBLINGAN KABUPATEN BULELENG BALI Fendria Wadi; Deny Suhernawan Yusup; Ni Made Suartini
SIMBIOSIS Vol 9 No 2 (2021)
Publisher : Jurusan Biologi Universitas Udayana

The purpose of this research was to determine the structure of the Tamblingan lake plankton community and community activities in the area around the lake and on the body. The methods used was survey methods and quantitative-descriptive methods. Survey method by visually observing the location based on the utilization of the lake area. The quantitative-descriptive method describes phenomena based on calculations and compared with ecological index. The results showed that the plankton composition consisted of five phyla dominated by the Chlorophyta phylum. Genus that was frequently found and in high abundance are Cosmarium, Staurastum, and Nitzshia. The abundance of plankton ranges from 5,757-11,197 cells/liter with the diversity index value (H') ranging from 1,38 to 1,76 which is classified as moderate, the uniformity index (E) ranges from 0.52-0.65 which is classified as moderate, the dominance index (C ) ranged from 0.35 to 0.48 indicating no dominance of a particular genus. Most of the community activities in the Tamblingan lake area are farmers and fishermen. Agricultural activity is very low so that it has no impact on the waters of Lake Tamblingan.

AKTIVITAS ANTIDIABETES EKSTRAK ETANOL DAUN BELIMBING BESI (Averhhoa carrambola) PADA MENCIT (Mus musculus) NI PUTU SRI PUSPITA WIDI YANTHI; Ni Putu Adriani Astiti; Ni Wayan Sudatri
SIMBIOSIS Vol 9 No 2 (2021)
Publisher : Jurusan Biologi Universitas Udayana

Senyawa antioksidan, flavonoid, dan saponin banyak terkandung dalam daun belimbing besi (Averrhoa carambola) sehingga tujuan dari penelitian ini untuk melihat pengaruh antidiabetes ekstrak daun belimbing besi pada mencit. Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan 6 perlakuan, yaitu P1 (aquadest tanpa aloksan) sebagai kontrol negatif, P2 (aquadest dengan aloksan) sebagai control positif, P3 metformin 500 mg/kgBB), P4 (aloksan + ekstrak daun belimbing besi 100 mg/kgBB) , P5 (aloksan + ekstrak daun belimbing besi 150 mg/kgBB), P6 (aloksan + ekstrak daun belimbing besi 200 mg/kgBB). Sebelumnya hewan coba dijadikan diabetes dengan menginduksi aloksan sebanyak 120 mg/kgBB secara intramuscular pada otot paha. Pembuatan ekstrak daun belimbing besi menggunakan metode maserasi. Setelah hewan diabetes, diberikan perlakuan ekstrak etanol daun belimbing besi sesuai rancangan percobaan, dimulai hari ke-7 sampai hari ke-21. Kadar glukosa darah diukur dengan glucometer setiap minggu. Hasil penelitian menunjukkan nilai penurunan kadar glukosa darah terbaik terjadi pada minggu pertama dan minggu kedua. Pada minggu pertama, perlakuan metformin mampu menurunkan glukosa darah mencit sebanyak 88.4%, sedangkan perlakuan ekstrak etanol daun belimbing besi dosis 200 mg sebanyak 60%. Pada minggu kedua, 65.8% untuk metformin, dan 63% untuk dosis ekstrak daun belimbing 200 mg/kg BB. Ekstrak etanol daun belimbing besi berpotensi sebagai obat antidiabetes. Kata Kunci: mencit, diabetes mellitus, glukosa darah, daun belimbing besi.

THE IMPACT OF LAUNDRY DETERGENT AS A METHOD OF WASHING TOWARDS SPERM DNA EXTRACTION RESULT ON COTTON FABRIC Christian Subagya Gunardi; I Ketut Junitha; Inna Narayani
SIMBIOSIS Vol 9 No 2 (2021)
Publisher : Jurusan Biologi Universitas Udayana

The presence of spermatozoa DNA is reliably counted as a solid evidence when dealing with rape cases. However, police department often finds difficulties to obtain it, mainly because it has been destroyed by the perpetrator. Such act can be done by washing semen stains on the clothing with detergent. The purpose of this research is to analyze the impact of detergent as a washing property towards spermatozoa DNA extract on a cotton fabric. Observed variables in this study are quantity and quality of spermatozoa DNA extract from semen stain on cotton fabric. Samples are divided based on two separate treatments; detergent-washed and unwashed. Furthermore, samples are stored with different storage time. This research applied two methods; DNA extraction using kit (Roche) and DNA quantification using spectrophotometer Nanodrop. The research was held at Biomedic Laboratory, Faculty of Medicine, Udayana University, Denpasar with ethical clearance. Seminal plasma sampel was obtained from a donor with informed consent. This research showed that seminal stain washing on cotton fabric doesn't remove the DNA until two-week-storage time.

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Location Kota denpasar, Bali INDONESIA

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Contact Name
-

Contact Email
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Journal Mail Official
-

Editorial Address
-

Location
Kota denpasar,

Bali

INDONESIA