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All Journal International Journal of Biosciences and Biotechnology METAMORFOSA Journal of Biological Sciences Buletin Udayana Mengabdi Jurnal Veteriner Jurnal Biologi Udayana Bumi Lestari Ecotrophic, Journal of Environmental Science SIMBIOSIS Jurnal Riau Biologia Indonesian Biodiversity Journal Indonesian Biodiversity Journal
Ni Wayan Sudatri
Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Veterinary

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Uji Teratogenik Ekstrak Minyak Biji Jintan Hitam (Nigella sativa) pada Mencit (Mus musculus l.) Bunting SETYAWATI, IRIANI; SUDATRI, NI WAYAN
Jurnal Riau Biologia Vol 1, No 1 (2016)
Publisher : Jurusan Biologi FMIPA Universitas Riau

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Abstract

This experiment was performed to examine the effects of black cumin (Nigella sativa) seed oil extract onreproduction performance and fetus development if given during organogenesis period. Forty pregnantmice were randomly divided into four groups. Extract was treated orally by gavage with doses 0%(control), 0.1 ml, 0.2 ml, and 0.3 ml since gestation day 6 to 15. Caesarean section were performed topregnant mice on day 18 to remove fetuses. Observation covered reproduction performance i.e. the foetusnumber (reabsorptions, dead/ alive foetus), and morphological of the foetus (weight and length of thelitter, and malformations). Statistical analysis was performed using Anova and Duncans Multiple RangeTest. Teratogenic effects of black cumin oil extract caused decreasing of reproductive performance of themother (alive foetus decreased, dead and resorbed foetus increased) and an increased incidence of birthdefects (fetal weight and length and fetal hemorrhage).Keywords: Nigella sativa, congenital malformations
THE DEVELOPMENT OF OVARIAN FOLLICLE CELLS AND CORPUS LUTEUM OF MICE (Mus musculus) SWISS WEBSTER GIVEN Leucaena leucocephala LEAF EXTRACT Ngurah Intan Wiratmini; Ni Wayan Sudatri; Iriani Setyawati
International Journal of Biosciences and Biotechnology Vol 3 No 1 (2015)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

This research was conducted to determine the effect of Leucaena leucocephala leaf extract on the development of ovarian follicle cells of mice (Mus musculus). Twenty four female mice, 10 weeks old, were divided into 4 groups. The control group (P0) were given 0.9% NaCl and the treatment group P1, P2 and P3 were given 0.5, 1 and 1.5 g/ kg bw of Leucaena leaf extract, respectively. The treatments were administered daily for 15 days by gavage. After the treatment ended, all mice were dissected to collect the ovaries. Histological preparation of the ovaries used paraffin method and Hematoxylin-Eosin staining. Observations were made on the number of primary, secondary, and tertiary oocytes, and also De Graaf follicles and corpus luteum. Data were statistically analyzed using One Way ANOVA method. The results showed there were no significantly differences (P>0.05) on the number of primary, secondary, and tertiary oocytes, as well as De Graaf follicles and corpus luteum among the control and treatment groups. The mimosine content of Leucaena leaf extract was 0.87% (less than 1%) so it could not inhibit the secretion of Follicle Stimulating hormone by the pituitary gland. The doses of Leucaena leaf extract used in this research did not significantly affect the follicles development of mice ovaries. In this study, the mimosine content of Leucaena leaf extract decreased 91.8%.
Uji Efektivitas Vitamin C sebagai Neuroprotektor pada Mencit (Mus musculus) yang Diinduksi Akrilamida Salsabillah Rahma; Ngurah Intan Wiratmini; Ni Wayan Sudatri
Metamorfosa: Journal of Biological Sciences Vol 9 No 1 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i01.p08

Abstract

Acrylamide is a contaminant compound found in several types of food containing carbohydrates and processed food through frying or roasting at high temperatures. Acrylamide compounds could cause the formation of oxidative stress which could lead to neurotoxicity. This oxidative stress could be minimized by administering the antioxidant vitamin C. This study was conducted to determine the mice cerebral cortex histology, the mice cerebellum histology induced by acrylamide and vitamin C supplementation, and the most effective dose as a neuroprotector. This study used a completely randomized design (CRD) with 4 groups, namely K + (acrylamide 12.5 mg / kg BW); K- (NaCl 0.9%); P1 (vitamin C dose of 0.26 mg/animal /day + acrylamide dose of 12.5 mg/kg BW); and P2 ((vitamin C dose of 0.52 mg/animal/day + acrylamide dose of 12.5 mg / kg BW) and 6 repetitions so that the number of mice used was 24. The acrylamide and vitamin C solutions were administered orally, and acrylamide given for 20 days, while vitamin C was given 3 days before acrylamide induction until day 20. Mice were dissected at day 21, brain organs were collected for histological preparations by the paraffin method and Hematoxylin-Eosin staining. The qualitative data obtained were analyzed by One Way ANOVA and continued with the Duncan test. The parameters observed were necrosis in pyramid cells and glial cells. The results showed that vitamin C at dose of 0.52 mg/animal/day significantly repaired the damage in pyramid cells and glial cells induced by acrylamide.
PENURUNAN FUNGSI HATI TIKUS BETINA (Rattus norvegivus L) YANG DIINJEKSI WHITE VITAMIN C DOSIS TINGGI DALAM JANGKA WAKTU LAMA DITINJAU DARI KADAR SGPT, SGOT SERTA GAMBARAN HISTOLOGI HATI Ni Wayan Sudatri; Iriani Setyawati; Ni Made Suartini; Dwi Ariani Yulihastuti
Metamorfosa: Journal of Biological Sciences Vol 3 No 1 (2016)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2016.v03.i01.p07

Abstract

This study aims to determine the side effects of high-dose injections of vitamin C in a long term against liver histology. This study used a completely randomized design (CRD) with different long treatments of vitamin C injection in various doses of P0 (control), P1 (30 days injection), P2 (50 days injection), P3 (70 days injection) and P4 (90 days injection). Parameters measured were plasma levels of SGPT and SGOT, the percentage of hydropic degeneration,  fatty degeneration, pyknotik nuclei, and necrosis of liver cells, and also inflammatory cell infiltration and sinusoid congestion. SGOT levels showed a significant differences (P=0,009) between the treatment and control group by ANOVA test. The Kruskal Wallis test showed that ALT levels were not significantly different (P=0,86), however, the levels of creatinine showed significant differences (P=0,002). The hydropic degeneration were not significantly different (P=0,054) by Kruskal Wallis test, whereas the fatty degeneration were significantly different (P=0,000) between the treatment and control groups by ANOVA test. Pyknotik nuclei and necrotic cells showed significant differences between the control and treatment with the P value of 0,016 and 0,01 respetively. The injected white vitamin C in high doses for long periods decreased the physiological functions of female mice liver.
DIVERSITAS SERANGGA PADA PERKEBUNAN PEPAYA (Carica papaya L.) DI DAERAH SANUR, DENPASAR, BALI Ni Made Suartini; Ni Wayan Sudatri; Ni Luh Watiniasih
Metamorfosa: Journal of Biological Sciences Vol 2 No 2 (2015)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

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Abstract

The role of insects in papaya plants is as pollinators, pests, parasites and predators of other insects. Referring to the role of insects, this research was conducted to determine the insects on an papaya plantations. Traping method using methyl eugenol and ripe papaya fruit as well as the manual method with insect net used in this research. Each method was repeated three times with an interval of sampling is one week. Insect netting done on the same day with traping method. Samples were preserved and then taken to the laboratory for identification. Environmental factors were measured are: temperature, humidity, latitude and altitude. Insects were identified by reference: Borror et al. (1992), CSIRO I & II (1991) and also Peggie and Amir (2006). Five species belonging to the genus Bactrocera found on methyl eugenol traps and five species belonging to the genus Drosophila was found in ripe papaya fruit traps as well as 16 other species found using insect netting around the papaya plant. In addition, there is also the genus Paracoccus on a papaya plant. Bactrocera papayae and genus Paracoccus is an insect pest on papaya plants.Keywords: diversity, insect, papaya plantation, Sanur
Diversitas Gastropoda pada Habitat Persawahan dengan Ketinggian Berbeda Ni Made Suartini; Ni Wayan Sudatri
Metamorfosa: Journal of Biological Sciences Vol 6 No 2 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i02.p11

Abstract

Gastropods or often called snails, live in various habitats including in rice fields habitat. There are several species of rice field gastropods which are detrimental because they damage the plants but some can be utilized. The presence of rice field gastropods is influenced by environmental factors. Environmental factors are certainly different at different altitudes, so that it might affect the species of Gastropods that exist. This study aims to determine the diversity of Gastropods found on rice fields at different altitude. Gastropod sampling were carried out in several rice fields with different altitudes, that is under 100 m, 100 m to 300 m and above 300 m to 500 m above the sea level. Sampling were done by making a 1mx1m square placed in each corner of the rice field and in the middle of the rice field. In addition to the rice fields, samples were also taken from irrigation channels that irrigated the fields. Gastropods found were six species where Lymnaea rubiginosa and Digoniostoma truncatum were only found in rice fields in each location and Terebia granifera was only found in irrigation channels. Melanoides tuberculata, Melanoides maculata and Pomacea canaliculata were found in rice fields and irrigation channels. Locations I and II have a moderate diversity index (1,360 and 1,408) and location III is low (0,795).
Prevalensi Leucocytozoonosis Dan Plasmodiosis Pada Itik (Anas plathyrynchos) Yang Dipelihara Dalam Skala Rumah Tangga Kadek Indah Kartika Sari; Ni Wayan Sudatri; Ni Made Suartini
Metamorfosa: Journal of Biological Sciences Vol 8 No 1 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i01.p06

Abstract

Leucocytozoon and Plasmodium are blood parasitic protozoa that can be found in poultry blood including ducks. These protozoa can cause Leucocytozoonosis and Plasmodiosis in poultry. Spread of the disease through different biological vectors. Similium sp. and Culicoides arakawae as vectors of Leucocytozoonosis and vectors of Plasmodiosis are Culex sp., Culiseta sp., and Aedes sp. This study aims to determine the prevalence of Leucocytozoonosis and Plasmodiosis in ducks that are maintained on a household scale. Ducks are taken from four locations (breeders on a household scale) in Besan Village. In each location, 5 ducks were taken so that the total number of ducks used was 20. This research used purposive sampling method. Samples of duck blood were made as blood smear preparations and stained with 10% Giemsa stain solution then observed under a microscope. The parameters observed were the percentage of blood samples infected with Leucocytozoon and Plasmodium and hematological parameters (total leukocyte count, total erythrocyte count, hemoglobin level, and PCV value). The results showed that Leucocytozoon and Plasmodium were not found in duck blood cells. The prevalence of Leucocytozoonosis and Plasmodiosis in ducks was 0%. The average value of duck blood profile through One Way Anova statistical analysis showed no significant difference between ducks from each sampling location (p> 0.05) and the value of duck blood profile for all samples was within the normal range.
Penurunan Kualitas Sperma Tikus (Rattus novergivus) yang Diinjeksi Vitamin C Dosis Tinggi dalam Jangka Waktu Lama Ni Wayan Sudatri; Dwi Ariani Yulihastuti; Ni Made Suartini
Metamorfosa: Journal of Biological Sciences Vol 6 No 1 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i01.p02

Abstract

Benefits of white vitamin C to increace the immune system is already exis. However, the use of high doses of vitamin C and in the long term can cause side effects.. The objective of this study was to determine the side effects of high-dose injections of vitamin C in the long term on sperm quality of rats. The design used in this study is completely randomized design (CRD) with long treatment injections of high doses of vitamin C are different. ie: P0 (control), P1 (injected 30 days), P2 (injected 50 days ), P3 (injected 70 days) and P4 (injected 90 days). Parameters measured were sperm quality (motility, viability, morphology, number) ,. The results showed that the sperm quality of rats (Rattus novergivus). Sperm quality of male rats (Rattus norvegivus L.) were injected vitamin C in high doses for long periods of time between the control and treatment groups showed significant differences (P = 0.000). The quality of sperm of mice injected high doses of white vitamin C tend to decrease sperm quality indicated by percentage of motility, viability, and morphology of spermatozoa although the concentration of spermatozoa tends to increase. Keywords: white vitamin C, rat, sperm quality
PELATIHAN PEMBUATAN BODY CREAM DAN LOTION LIDAH BUAYA (ALOE VERA) DI PURI DAMAI DESA SINGAKERTA KECAMATAN UBUD KABUPATEN GIANYAR BALI N.L. Arpiwi; I.K. Muksin; I.G.A.S. Wahyuni; I.K. Ginantra; N.W. Sudatri
Buletin Udayana Mengabdi Vol 18 No 4 (2019): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (421.357 KB) | DOI: 10.24843/BUM.2019.v18.i04.p02

Abstract

Lidah buaya (Aloe vera) adalah tanaman sukulen dari keluarga Asphodelaceae yang tersebar luas di daerah tropis dan sub-tropis. Tanaman ini sangat populer karena memiliki banyak kegunaan, misalnya sebagai pelembab kulit, pertumbuhan rambut, dan pengendalian jerawat. Tujuan dari pelatihan ini adalah untuk memberikan informasi kepada masyarakat Desa Singakaerta, Kabupaten Gianyar, tentang bagaimana mengolah lidah buaya menjadi body cream dan lotion sehingga dapat meningkatkan nilai ekonomi tanaman tersebut. Prosedur kerja dimulai dengan mengupas daun lidah buaya untuk mendapatkan pulp, lalu diblender dan disaring untuk mendapatkan jus. Basis cream dan lotion terdiri dari minyak kelapa murni (VCO), cethyl alcohol, asam stearat, gliserin, triethanolamine, nipagin, nipasol dan aquades dengan jumlah sesuai formulasi. Fase air dicampur dengan fase minyak pada suhu 70oC sambil diaduk sampai homogen dan kemudian ditambahkan jus lidah buaya pada suhu 40oC. Aroma cendana ditambahkan untuk body creamdan aroma melati ditambahkan ke body lotion pada suhu 30oC. Body cream berwarna putih susu, aroma cendana, tekstur lembut, agak kental, pH 6,5, homogen dengan viskositas 95 poise. Body lotion berwarna putih susu, aroma melati, tekstur lembut, lebih encer, pH 7, homogen dengan viskositas 39 poise. Kata kunci : Body cream, body lotion, Aloe vera, VCO
UPAYA MENINGKATKAN KEMAMPUAN PROBLEM SOLVING SISWA SD ADHI MEKAR INDONESIA MELALUI PEMBELAJARAN MATEMATIKA BERBASIS EKSPLORASI I. Harini; I G.S. Astawa; N.W. Sudatri; I G.A.P. Adnyana; I M. Widiartha
Buletin Udayana Mengabdi Vol 16 No 2 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Abstract

Diantara semua mata pelajaran SD, pembelajaran matematika tetap menjadi topik yang paling sering muncul sebagai pembelajaran yang paling bermasalah dalam proses belajar mengajarnya. Kesiapan guru dalam mengajar dan kurang minat siswa dalam belajar matematika merupakan dua masalah penting yang harus diatasi. Untuk membuat pembelajaran matematika lebih bermakna bagi siswa, maka pengintegrasian mata pelajaran matematika dengan masalah-masalah nyata sangat penting. Salah satu alternatif yang dapat ditempuh untuk menyelesaikan masalah tersebut adalah dengan merancang pembelajaran matematika yang berbasis eksplorasi. Kegiatan pengabdian ini menggandeng SD Adhi Mekar Indonesia sebagai sekolah mitra. Adapun tujuan yang ingin dicapai melalui kegiatan ini diantaranya: (1) guru dapat mengembangkan perangkat pembelajaran matematika yang lebih inovatif berbasis eksplorasi (2) melihat sejauh mana peningkatan kemampuan problem solving siswa melalui pembelajaran berbasis eksplorasi. Dari hasil analisis tabulasi silang antara nilai pre test dan post test menunjukkan bahwa pemberian pelatihan pembuatan bahan ajar berbasis eksplorasi berpengaruh signifikan terhadap peningkatan kemampuan guru dalam membuat bahan ajar berbasis eksplorasi.
Co-Authors A.A.S. A. Sukmaningsih Anak Agung Gde Raka Dalem Dwi Ariani Yulihastuti G.A.A Rina Febrina I G.A.P. Adnyana I G.S. Astawa I Gede Agus Pradana Putra I Gusti Ayu Intan Warmadewi I GUSTI AYU MANIK ERMAYANTI I Gusti Ayu Manik Widhyastini I Ketut Ginantra I KETUT MUKSIN I M. Widiartha I Putu Ari Wijana Dipa I Wayan Andi Yoga Kurniawan I Wayan Wena Diartha I. Harini I.G.A.S. Wahyuni Ida Bagus Gede Darmayasa Iriani Setyawati Iriani Setyawati Iriani Setyawati IRIANI SETYAWATI Iriani Setyawati Kadek Indah Kartika Sari Kadek Mardika LUH Puru ESWARYANTI KUSUMA YUNI MADE PHARMAWATI Ngurah Intan Wiratmini Ni G. A. Manik Ermayanti Ni LUH ARPIWI Ni Luh Watiniasih Ni Made Rai Suarni Ni Made Suartin Ni Made Suartini Ni Nyoman Wirasiti Ni Putu Adriani Astiti Ni Putu Sinta Puspa Dewi NI PUTU SRI PUSPITA WIDI YANTHI Putu Ayudina Asti Puspita Retno Kawuri Salsabillah Rahma SANG KETUT SUDIRGA