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Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati

biota
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Published by Universitas Atma Jaya Yogyakarta

ISSN : 25273221 EISSN : 2527323X DOI : doi.org/10.24002/biota

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati merupakan jurnal ilmiah yang memuat hasil-hasil penelitian, kajian-kajian pustaka dan berita-berita terbaru tentang ilmu dan teknologi kehayatian (biologi, bioteknologi dan bidang ilmu yang terkait). Biota terbit pertama kali bulan Juli 1995 dengan ISSN 0853-8670. Biota terbit tiga nomor dalam satu tahun (Februari, Juni, dan Oktober).

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 22 Documents

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Search results for , issue "Vol 13, No 3 (2008): October 2008" : 22 Documents clear

Struktur Populasi Genetik Ayam Hutan Hijau Menggunakan Sekuen Hypervariable 1 D-Loop DNA Mitokondria Arifin Zein, M. Syamsul; Sulandari, Sri
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 3 (2008): October 2008
Publisher : Universitas Atma Jaya Yogyakarta

Thirty-three individuals from four populations of green junglefowl (Gallus varius) were collected throughout Indonesia: 14 from Central Java, 4 from Eastern Java, 3 from Sumbawa island and 12 from Flores island. The mitochondrial DNA D-loop the samples were analysed for sequence diversity. Twenty-five haplotypes with 28 polymorphic sites were identified within the first hypervariable-1 fragment (397 bp) of the D-loop. Fuâ€™s Fs value was -25.96 (all samples, P=0), indicating high genetic diversity and population expansion; a conclusion supported by a neighborâ€“joining analysis of the haplotypes. Though sample size per population varied between 3 and 14, the Fs values for the four populations, between -2.20 and -10.76, were all significant (P=0). Only one haplotype was shared between three populations (Central Java, Sumbawa island, and Flores island) by a total of seven individuals. Within populations, only three haplotypes were shared by three individuals. The remaining haplotypes were unique, indicating genetic differentiation between populations as confirmed by significant pairwise Fst values at P=0.05 in four out of the six population pairs (except two pairs of Central Java & Sumbawa island and Flores island & Sumbawa island). The diversity pattern observed at the mtDNA of the green junglefowl provides a baseline which may help to understand the recent population expansions of domesticated chickens from multiple centres of domestications. Our observations also suggest careful interpretation of the results of genetic characterization may be needed when applied to the management and conservation of species like the green junglefowl. As in other multiparous birds and mammals with a short reproductive interval, green junglefowl may have established distinct genetic entities in metapopulations across its geographic distribution.

Pemanfaatan Î±-Tokoferol untuk Meningkatkan Profil Superoksida Dismutase (SOD) Ginjal Tikus di Bawah Kondisi Stres Wresdiyati, Tutik; Astawan, Made; Fithriani, Diini; Adnyane, I Ketut Mudite; Hidayati, Mustika
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 3 (2008): October 2008
Publisher : Universitas Atma Jaya Yogyakarta

Stress condition has been reported decreased the level of intracellular antioxidant, especially superoxide dismutase (SOD). The condition can lead to certain cell impairment and finally can reach to the degenerative processes, such as aging and carcinogenesis. We need several alternative treatments to solve the severe problem under antioxidant defiency status caused by stress condition. The aim of the study was to observe the effect of Î±-tocopherol on increasing the profile of superoxide dismutase (SOD) in the kidney of rats under stress condition. The stress condition was achieved by five days fasting together with smimming for 5 min/day and only drinking distiled water ad libitum. Î±-tocopherol was orally administrated at a dose of 60 mg/Kg/BW/day for seven days. By measuring SOD activity and Cu,Zn-SOD imunohistochemically, showed that Î±-tocopherol has effect on increasing the profile of intracellular antioxidant in the rats kidney tissue under stress condition.

Produksi Rennet Mucor pusillus yang Ditumbuhkan pada Limbah Padat Tapioka (Onggok) Choliq, Abdul
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 3 (2008): October 2008
Publisher : Universitas Atma Jaya Yogyakarta

The production of rennet enzyme extracted from Mucor pusillus in â€œonggokâ€ medium the effect of incubation peptone and ammonium nitrate to enzyme production, and the effect of pH and temperature to the activities of rennet were found. The statistical method used is Completed Randomized Design with 3 replicates for each treatment, with the rennet activity in RU/ml enzyme extract. The rennet production was conducted in erlenmeyer (100 ml) that contain 17.5 g â€œdry onggokâ€ and 50 ml solution of suspension used. The effect of incubation time was detected in three incubation times (4, 7, and 10 day), while effect of peptone and ammonium nitrate to the production of the rennet enzyme was detected in 4 concentrations (0, 1, 2, and 3%) for 7 days of incubation times. The effect of pH to the activity of rennet from the treatment of adding peptone 1% with 7 days incubation time was detected in 5 pH variation (5, 5.5, 6, 6.5 and 7) while the effect of temperature were detected in 5 variations (30, 35, 40, 45, and 50oC). The research results showed that the time of incubation, the addition of peptone and ammonium nitrate affected significantly to enzyme production of rennet Mucor pusillus in â€œonggokâ€ medium. The highest production of the rennet enzyme products was in the addition of peptone 1% (12.53 RU/ml) with 7 days incubation time. On the treatment of temperature and pH, the optimum activity of rennet Mucor pusillus was in pH 5.5 (40.03 RU/ml) and the activity of rennet Mucor pusillus increased up to temperature of 50oC.

Inventarisasi Jenis dan Distribusi Habitat Rotan pada Hutan Dataran Tinggi dan Dataran Rendah di Kalimantan Selatan Arifin, Yudi Firmanul
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 3 (2008): October 2008
Publisher : Universitas Atma Jaya Yogyakarta

Rattans are spiny climbing palms in the Old World tropics and subtropics. Some species are the source of cane for the cane furniture industry in Indonesia. The large number of rattan species and their wide geographical range is matched by great ecological diversity. The restriction of rattan species to different climatic zones and altitudinal range suggests that these species may have precise climatic requirements and also altitudes. The research was conducted on upland and lowland of natural forest in South Kalimantan. The data collection used survey with transect method. Volume of rattans in upland forest was relatively lower than lowland forest. In altitudinal range in South Kalimantan, rattans were found from sea-level up to 870 m, the highest altitude record being held by Calamus javensis, Daemonorops micracantha, Calamus pilocellus, and Calamus sp. (rotan tunggal). While the lowest altitude record was occupied by Calamus trachycoleus. Rattans in natural forest require light intensity, humidity and temperature were 18â€“ 65%, 60 â€“ 70%, 28 â€“ 33oC, respectively.

Keragaman dan Penyebaran Benalu pada Tanaman Koleksi di Kebun Raya Cibodas, Jawa Barat Uji, Tahan; Sunaryo, Sunaryo
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 3 (2008): October 2008
Publisher : Universitas Atma Jaya Yogyakarta

A study on mistletoe has been carried out in Cibodas Botanical Garden,West Java. The aim of the study were to know mistletoe diversity which have attacked many collection plants species in the area. Result of the study indicated that there were 5 species mistletoe (i.e. Dendrophthoe pentandra, Scurrula oortiana, Macrosolen cochinchinensis, Lepeostegeres gemmiflorus and Viscum articulatum) and they attacked 91 collection plants species in Cibodas Botanical Garden. D. pentandra was reported as the most abudance mistletoe. Collection plants species of familes Moraceae and Myrtaceae were also reported as the highest of collection plants species have been parasited by mistletoe.

Kemampuan Mikroorganisme Efektif dalam Mengolah Limbah Cair Pabrik Spiritus Lestari, H. N.; Atmodjo, P. Kianto; Sidharta, B. R.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 3 (2008): October 2008
Publisher : Universitas Atma Jaya Yogyakarta

Proses produksi suatu industri senantiasa menghasilkan limbah yang harus diolah dengan baik agar tidak membahayakan lingkungan. Pabrik spiritus â€œXâ€ di Yogyakarta telah mengolah limbah cairnya secara fikokimiawi, namun hasilnya masih kurang baik. Karakteristik limbah pabrik ini adalah berbentuk cair, berwarna hitam, bau, keruh, kandungan BOD 2.978 mg/l dan COD 103.433 mg l, sehingga nilainya sangat jauh di atas baku mutu (Kepmen: 03/men LH/I/1998). Untuk itu perlu dilakukan usaha-usaha mengolah lebih lanjut agar mutu limbah semakin mendekati baku mutu. Salah satu usahanya adalah mengolah lebih lanjut dengan metode biologi.

Produksi Metabolit Utama (-)-Citrinin, pada Kultur Jamur Endofit Penicillium sp dari Tanaman Teh Agusta, Andria; Jamal, Yuliasri
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 3 (2008): October 2008
Publisher : Universitas Atma Jaya Yogyakarta

Endophytic fungi have been recognized as source of broad range biological active metabolites with high chemical structure diversity. The purpose of this research is to isolate and characterize major metabolite produce by the endophytic fungi Penicillium sp isolated from a tea plant. Cultivation of endophytic fungus Penicillium sp (AB2245443) in liquid medium PDB on a rotary shaker at 100 rpm, temperature 25 â€“ 30oC for 7 days, produced a yellow metabolite. Separation of metabolite through chromatography technique and followed by chemical structure elucidation based on MS, IR, NMR spectra and published data showed that the yellow metabolite is (-)-citrinin.

Seleksi, Karakterisasi dan Identifikasi Bakteri Pendegradasi 2-(thiocyanomethylthio) benzothiazole (TCMTB) Sembiring, Langkah; Susilawati, Lela; Suhartanti, Dwi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 3 (2008): October 2008
Publisher : Universitas Atma Jaya Yogyakarta

The objective of this research was to investigate the capabilities of bacteria isolated from industrial tanning waste to degrade TCMTB. The bacteria was initialy screened, based on their tolerance to various concentration of TCMTB using paper disk method. Then, those strains were further analyzed in terms of their ability to produce ammonia (NH4+) and sulphate (SO42-). Degradation activity was measured based on remaining residue of TCMTB analyzed using HPLC. The superior strain that showed the highest activity in degradation of TCMTB then were characterized and identified based on phenotypic and 16S rDNA sequence analysis. The result of the experiments showed that four selected strains among seven were choosen based on their high tolerance to various concentration of TCMTB, namely PK1, PK2, PK4 and PK6. All four strains showed the ability to produce ammonia and sulphate but three of which, namely PK2, PK4 and PK6 showed the high capability to degrade TCMTB. One particular strain (PK2) was observed to degrade TCMTB 40.8% within 7 days, but the others were less than 30%. Based on the phenotypic characteristics and 16S rDNA sequence analysis, the best strains (PK2) was identified to be member of genus Pseudomonas.

Komposisi Guild dan Lebar Relung Burung Strata Bawah di Sipisang, Sumatera Barat Novarino, Wilson; Mardiastuti, Ani; Prasetyo, Lilik B.; Widjakusuma, Reviany; Mulyani, Yeni A.; Kobayashi, Hiroshi; Salsabila, Anas; Jarulis, Jarulis; Janra, M. Nazri
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 3 (2008): October 2008
Publisher : Universitas Atma Jaya Yogyakarta

Guild composition and niche breadth are important point on avian studies. This paper describes the guild composition and niche breadth of understorey bird in Sipisang, West Sumatra. The study was conducted since May 2002 until October 2004 for approximately 10 days each month (totally 284 days or 51.120 net.hours). Fifteen mist nets were operated on ground level separately on three locations, which made 60 m line each. Mist nets were operated from 6.00 AM until 18.00 PM, and checked every two hours. The captured birds were identified, ringed, measured, weighted, photographed and released. In total 1061 individuals were captured during the study; those birds belong to 103 Species and 28 families. Based on guild, study area was dominated by insectivore-frugivore birds (IF) both in species and individual level. Meanwhile terrestrial frugivorous (TF) became the lowest one. Insectivore-frugivore also recorded has the broadest niche breadth. The bird communities dominated by small birds, which have weight lower than 30 g.

Kondisi Optimum untuk Produksi Kitinase dari Streptomyces Rkt5 dan Karakterisasi pH dan Suhu Enzim Yurnaliza, Yurnaliza; Margino, Sebastian; Sembiring, Langkah
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 3 (2008): October 2008
Publisher : Universitas Atma Jaya Yogyakarta

Chitinase is chitin degrading enzyme which is produced by Streptomyces Rkt 5 is isolated microorganism from peanut rhizosfer. This enzyme and its microorganism can be used in many agricultural, medicine and industrial purposes. The aim of the research was to find out the optimum condition for production of chitinase and to characterize of pH and temperature to chitinase activity. Optimalizing production the research had 4 treatments. The optimum conditions were achieved at mineral liquid medium containing with chitin 0,2% (w/v) as inducer, 10% (v/v) inoculum, pH 7 and 48 hours incubation. The crude enzyme was partially purified by salting out with 70% ammonium sulfate resulted in 3.31 time more purity enzyme than the crude one. This enzyme had maximum activity at 50oC and pH 5.5.

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Brigitta Laksmi Paramita

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brigitta.laksmi@uajy.ac.id

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+6282329549978

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journal.biota@gmail.com

Editorial Address
Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia

Location
Kota yogyakarta,

Daerah istimewa yogyakarta

INDONESIA

Abstract

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Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Brigitta Laksmi Paramita

Contact Email brigitta.laksmi@uajy.ac.id

Phone +6282329549978

Journal Mail Official journal.biota@gmail.com

Editorial Address Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia

Location Kota yogyakarta, Daerah istimewa yogyakarta INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Brigitta Laksmi Paramita

Contact Email
brigitta.laksmi@uajy.ac.id

Phone
+6282329549978

Journal Mail Official
journal.biota@gmail.com

Editorial Address
Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia

Location
Kota yogyakarta,

Daerah istimewa yogyakarta

INDONESIA