cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Brigitta Laksmi Paramita
Contact Email
brigitta.laksmi@uajy.ac.id
Phone
+6282329549978
Journal Mail Official
journal.biota@gmail.com
Editorial Address
Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia
Location
Kota yogyakarta,
Daerah istimewa yogyakarta
INDONESIA
Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Published by Universitas Atma Jaya Yogyakarta
ISSN : 25273221     EISSN : 2527323X     DOI : doi.org/10.24002/biota
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati merupakan jurnal ilmiah yang memuat hasil-hasil penelitian, kajian-kajian pustaka dan berita-berita terbaru tentang ilmu dan teknologi kehayatian (biologi, bioteknologi dan bidang ilmu yang terkait). Biota terbit pertama kali bulan Juli 1995 dengan ISSN 0853-8670. Biota terbit tiga nomor dalam satu tahun (Februari, Juni, dan Oktober).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 16 Documents
 1   2 
Search results for , issue "Vol 14, No 3 (2009): October 2009" : 16 Documents clear
Analisis Ekspresi Gen Selenometil Transferase pada Isolat Bakteri Termofilik Geobacillus 20K dan Thermomicrobium 14Ka sebagai Sumber Selenoprotein Triana, Evi; Nurhidayat, Novik; Rahayu, Sri Hartin
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 3 (2009): October 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (256.614 KB) | DOI: 10.24002/biota.v14i3.2580

Abstract

Selenium is a trace element that has essential nutrition value for human. Besides its nutritional value, it has important health benefits, including being a cancer chemoprotective agent. Methylated form of selenium is the most effective compound against cancer cells. Selenomethyl transferase (SMT) is responsible for methylating of selenium. This enzyme is coded by selenomethyl transferase (smt) gene which was found only from selenium accumulator plant, Astragalus bisulcatus. Thermophilic bacteria Thermomicrobium 14Ka and Geobacillus 20K have ability to accumulate selenium as well and potential in fighting cancer cells. Therefore a study to determine smt gene and its expression in both bacteria had been conducted in order to develop natural product of seleno-metilselenosistein for cancer treatment. The result showed that Thermomicrobium 14Ka and Geobacillus 20K have putative smt (selenomethyl transferase) gene, and such gene was expressed at different intensity. Geobacillus 20k expressed smt gene at higher intensity than Thermomicrobium 14k. Therefore, it is presumable that Geobacillus has a significant role in cancer remedy, meanwhile Thermomicrobium plays an essential role as cancer protective agent.
Expression and Characterization of Recombinant Protein of J-SU pGEX either by Single or Double Cell Lysis Margawati, Endang Tri; Ridwan, Muhamad
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 3 (2009): October 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (201.705 KB) | DOI: 10.24002/biota.v14i3.2579

Abstract

Penelitian ini dimaksudkan untuk optimasi produk protein rekombinan Superficial Unit dari virus Jembrana (JSU) yang dieksperikan melalui pemecahan sel secara tunggal dan ganda dengan sistem pGEX dalam skala flask 100ml media kultur. Dua metode pemecahan sel yang digunakan yaitu Freeze and Thaw (FT) sebagai pemecahan tunggal dan gabungan FT dan Sonikasi sebagai pemecahan ganda. Sel inang (E. Coli pembawa konstruk JSU pGEX) ditumbuhkan dengan induksi IPTG pada 37oC dengan pengocok berkecepatan 200rpm sampai mencapai kepadatan sel 0,8. Sel atau pelet dikoleksi dengan sentrifugasi, pelet dipecah dengan 2 perlakuan pemecahan sel tunggal dan ganda. Hasil pemecahan sel disentrifugasi untuk dikoleksi peletnya sebagai inclusion body. Solubilisasi dilakukan terhadap inclusion body dengan solubilisasi buffer dan diperoleh substrat protein JSU kemudian dimurnikan melalui Gluthation sepharose 4B (500μl resin) dengan metode batch capture. Hasil karakterisasi dengan SDS PAGE dan Western Blotting menunjukkan ukuran protein JSU pGEX yang tepat yaitu 60kDa pada kedua sistem pemecahan sel. Namun demikian, pemecahan sel secara tunggal menghasilkan protein murni JSU pGEX lebih besar (0.812ng/ul) dibanding pemecahan sel secara ganda (0.486ng/ul). Dari penelitian ini dapat disimpulkan bahwa protein rekombinan JSU pGEX terekspresi lebih baik dengan metode pemecahan sel Freeze and Thaw.
High Prevalence Level of Avian Malaria in the Wild Population of the Java Sparrow Yuda, Pramana
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 3 (2009): October 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (226.109 KB) | DOI: 10.24002/biota.v14i3.2583

Abstract

Java sparrow (Padda oryzativa) is anendemic bird to Java and Bali. It used to be avery common bird, but due to over exploitationthe bird has declined and been classified asVulnerable (BirdLife International, 2001). InIndonesia bird-keeping is a popular pastime,with deep cultural roots (Jepson and Ladle,2005). It is widely assumed that the hobbynegatively affects wild populations of commonas well as threatened birds (Jepson and Ladle,2005; Nash, 1994), such as Java sparrow.
Uji Patogenisitas Isolat Bakteri Indigenous (Bacillus thuringiensis) terhadap Serangga Hama Kubis (Crocidolomia binotalis Zell) Salaki, Christina L.; Situmorang, Jesmandt; Sembiring, Langkah; Handayani, Niken
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 3 (2009): October 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (200.033 KB) | DOI: 10.24002/biota.v14i3.2582

Abstract

Pathogenicity of 34 indigenous B. thuringiensis isolates against C. binotalis were determined. The pathogenicity test was conducted by using leaf dipped method with various spore concentrations. Third instar larvae of C. binotalis were used as insect test. Mortality data of test larvae were used to determine the pathogenicity of the isolates in terms of 72 hours LC50 by using probit analysis. The results of experiments showed YPPA 1. was the most pathogenic isolate, producing 72 hours LC50 = 9.5 x 103 spore.ml-1 with LT50 (1.5 x 107 spore.ml-1) of 24.6 hours while the ACH 2.3 was found to be the least pathogenic isolate with 72 hours LC50 = 2.3 x 106 spore.ml-1 and LT50 (1.5 x 107 spoore.ml-1) of 40.7 hours. The shortest LT50 (1.5 x 107 spore.ml-1 was found to be 18.2 hours produced by TUS.1 with 72 hours LC50 = 3.9 x 105 spore.ml-1 whereas the longest LT50 (1.5 x 107 spore.ml-1) was found tobe 83.2 hours produced by the SLK 4.1 with 72 hours LC50 = 3.1 x 104 spore.ml-1. Therefore, it can be concluded that both YPPA.1 and TUS.1 isolates are potential candidate to be developed for biological control agent.
Penurunan Glukosa dan Perubahan Profil Lipida Serum Tikus Sprague- Dawley Hiperglikemia-Hiperkolesterolemia Akibat Asupan Sorbitol-Oleat Poliester (SOPE) Murdiati, Agnes; Wuryastuty, Hastari; Marsono, Y.; Harmayani, Eni
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 3 (2009): October 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (343.509 KB) | DOI: 10.24002/biota.v14i3.2576

Abstract

Disorder of carbohydrate metabolism in the Diabetes Mellitus patients resulted in increasing of cholesterol synthesis. This condition increases the risk of heart attack or coronary heart disease, therefore it is recommended to lower fat intake. One of the alternatif to lower fat intake is by replacing fat with fat replacer in the diet. The aim of the present study is to evaluate the effect of replacing 75% of fat with SOPE on the glucose and lipid profile in diabetic-hipercholesterolemic rats. Eihgteen Sprague Dawley rats were devided into 2 groups of 9 rats. They were fed with hipercholesterol diet and were diabetic induced with alloxan IM injection. One group was fed with 75% SOPE substitute the fat in the standard diet and the other group was fed with standard diet. The result indicates that the oil substitution of standard diet of Sprague Dawley rats for sorbitol-oleic polyester as much as 75% gives an advantage to rats which suffer hiperglikemic-hypercholesterolemic because it decreased the glucose, total cholesterol, LDL cholesterol, and LDL/HDL cholesterol ratio, and did not decrease the HDL cholesterol, but increased triglyceride serum.
Construction of Soil Metagenomic Library to Obtain Recombinant Clones with an Indigenous Lipase Activity Susanto, Agus Hery; Pramono, Hendro; Lestari, Puji
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 3 (2009): October 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (350.658 KB) | DOI: 10.24002/biota.v14i3.2577

Abstract

Penelitian ini bertujuan untuk mengonstruksi perpustakaan metagenom tanah dari sekitar lokasi industri pengolahan minyak kelapa di Kabupaten Banyumas, Jawa Tengah, dalam upaya mendapatkan klon rekombinan dengan aktivitas lipase indigenuos. Penelitian dilakukan di Laboratorium Genetika, Fakultas Biologi, Universitas Jenderal Soedirman mulai bulan Mei hingga Desember 2006. Secara umum tahapan penelitian terdiri atas ekstraksi dan pemurnian DNA tanah, isolasi pUC19, pemotongan DNA tanah, pemotongan dan defosforilasi pUC19, ligasi fragmen-fragmen DNA tanah ke dalam pUC19 untuk menghasilkan molekul DNA rekombinan, dan transformasi sel inang E. coli JM109 menggunakan molekul DNA rekombinan yang diperoleh untuk mengonstruksi perpustakaan metagenom tanah. Pemotongan DNA tanah menghasilkan fragmen-fragmen yang sebagian besar berukuran lebih kurang 5 kb sehingga gen lipase dimungkinkan untuk dibawa di dalamnya. Transformasi sel inang E. coli JM109 memperlihatkan efisiensi 1.7 x 105 cfu/μg plasmid. Di antara koloni-koloni yang diperoleh, terdapat 102 koloni berwarna putih, yang menunjukkan keberadaan molekul DNA rekombinan. Koloni putih ini dapat digunakan sebagai materi untuk skrining klon rekombinan dengan aktivitas lipase.
Karakterisasi α-Amilase dari Aspergillus versicolor 3a1 yang Diproduksi pada Media Limbah Cair Tapioka Aini, Fitratul; Mubarik, Nisa Rachmania; Manaf, Lisdar A.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 3 (2009): October 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (146.985 KB) | DOI: 10.24002/biota.v14i3.2581

Abstract

The aim of this experiment was to characterize A. versicolor 3a1 α-amylase produced on cassava liquid waste media. Two types of media, base and combination media, were used as a comparison. Cassava liquid waste media contains 1% cassava starch, 1% yeast extract, 0.13% KH2PO4, and 0.05% MgSO4 diluted in cassava liquid waste. Base media contains same composition but using aquadest as a solvent, and combination media using mixture of aquadest and cassava liquid waste. A. versicolor 3a1 α-amylase showed its maximum specific activity in cassava liquid waste, base, and combination media after 3, 7, and 4 days incubation, respectively. Crude extract of α-amylase fromA. versicolor 3a1 was precipitated in 20-80% (w/v) ammonium sulphate. Precipitation of A. versicolor 3a1 α-amylase with 70% (w/v) ammonium sulphate on cassava liquidwaste, 60% on base media, and 60% on combination media will increase its specific activity 16.6, 4.28, and 5.65 times, respectively, compared to the specific activities ofcrude before precipitation. α-Amylase crude extract from A. versicolor 3a1 from all media showed its highest specific activity at 70oC and pH 5.0, and addition of FeSO4 increased the specific activity. Precipitated A. versicolor 3a1 α-amylase from all media showed its highest specific activity at 70oC and pH 6.0. Addition of FeSO4 precipitated 3a1 α-amylase from base and combination media will increase its specific activity, while MgSO4 will increase its specific activity in cassava liquid waste media. Thermostability assay revealed that the crude and the precipitated 3a1 α-amylase were relatively stable at 70oC up to 180 minutes incubation, except for precipitated3a1 -amylase on cassava waste media. Crude α-amylase 3a1 was relatively stable at pH 5-9 up to 1 hour incubation with wide pH ranges, while the precipitated with narrow pH ranges.
Seasonal Activity of Metabolic Enzymes of Littorina littorea (Gastropoda: Mollusca) Kaligis, G. J. Fontje
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 3 (2009): October 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v14i3.2578

Abstract

Aktivitas musiman enzim pyruvate kinase (PK), dan citrate synthase (CS) pada otot kaki siput, Littorina littorea (Gastropoda, Littorinidae) telah diteliti. Enzim-enzim ini berperan sebagai enzim kunci pada rangkaian metabolisme (metabolic pathways). Perbedaan yang signifikan terlihat pada aktivitas adalah CS dan PK sepanjang musim. Aktivitas spesifik dari CS bervariasi antara 0,04 dan 0,4 μg berat basah dan PK antara 0,1 dan 1,7 μg berat basah. Aktivitas enzim tersebut lebih tinggi pada musim dingin dibandingkan dengan musim panas. Hubungan negatif antara suhu dan aktivitas enzim terdeteksi dalam penelitian ini. Terdapat perbedaan yang nyata antara suhu air dan aktivitas enzim CS, dan suhu air dan udara pada enzim PK (P<0.05). Teramati tendensi yang sama untuk prosentasi perubahan pada aktivitas enzim PK dan CS pada bulan pertama pengamatan dan bulan berikutnya. Hasil penelitian setuju dengan hipotesis yang mengatakan bahwa terdapat kemungkinan perubahan aktivitas enzim CS dan PK selama siklus musiman. Fenomena ini dibahas dalam pembahasan tulisan ini.
Penurunan Glukosa dan Perubahan Profil Lipida Serum Tikus Sprague- Dawley Hiperglikemia-Hiperkolesterolemia Akibat Asupan Sorbitol-Oleat Poliester (SOPE) Agnes Murdiati; Hastari Wuryastuty; Y. Marsono; Eni Harmayani
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 3 (2009): October 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v14i3.2576

Abstract

Disorder of carbohydrate metabolism in the Diabetes Mellitus patients resulted in increasing of cholesterol synthesis. This condition increases the risk of heart attack or coronary heart disease, therefore it is recommended to lower fat intake. One of the alternatif to lower fat intake is by replacing fat with fat replacer in the diet. The aim of the present study is to evaluate the effect of replacing 75% of fat with SOPE on the glucose and lipid profile in diabetic-hipercholesterolemic rats. Eihgteen Sprague Dawley rats were devided into 2 groups of 9 rats. They were fed with hipercholesterol diet and were diabetic induced with alloxan IM injection. One group was fed with 75% SOPE substitute the fat in the standard diet and the other group was fed with standard diet. The result indicates that the oil substitution of standard diet of Sprague Dawley rats for sorbitol-oleic polyester as much as 75% gives an advantage to rats which suffer hiperglikemic-hypercholesterolemic because it decreased the glucose, total cholesterol, LDL cholesterol, and LDL/HDL cholesterol ratio, and did not decrease the HDL cholesterol, but increased triglyceride serum.
Construction of Soil Metagenomic Library to Obtain Recombinant Clones with an Indigenous Lipase Activity Agus Hery Susanto; Hendro Pramono; Puji Lestari
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 3 (2009): October 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v14i3.2577

Abstract

Penelitian ini bertujuan untuk mengonstruksi perpustakaan metagenom tanah dari sekitar lokasi industri pengolahan minyak kelapa di Kabupaten Banyumas, Jawa Tengah, dalam upaya mendapatkan klon rekombinan dengan aktivitas lipase indigenuos. Penelitian dilakukan di Laboratorium Genetika, Fakultas Biologi, Universitas Jenderal Soedirman mulai bulan Mei hingga Desember 2006. Secara umum tahapan penelitian terdiri atas ekstraksi dan pemurnian DNA tanah, isolasi pUC19, pemotongan DNA tanah, pemotongan dan defosforilasi pUC19, ligasi fragmen-fragmen DNA tanah ke dalam pUC19 untuk menghasilkan molekul DNA rekombinan, dan transformasi sel inang E. coli JM109 menggunakan molekul DNA rekombinan yang diperoleh untuk mengonstruksi perpustakaan metagenom tanah. Pemotongan DNA tanah menghasilkan fragmen-fragmen yang sebagian besar berukuran lebih kurang 5 kb sehingga gen lipase dimungkinkan untuk dibawa di dalamnya. Transformasi sel inang E. coli JM109 memperlihatkan efisiensi 1.7 x 105 cfu/μg plasmid. Di antara koloni-koloni yang diperoleh, terdapat 102 koloni berwarna putih, yang menunjukkan keberadaan molekul DNA rekombinan. Koloni putih ini dapat digunakan sebagai materi untuk skrining klon rekombinan dengan aktivitas lipase.

Page 1 of 2 | Total Record : 16

 1   2 

Filter by Year

2009 2009


Reset
Filter By Issues
All Issue Vol 8, No 3 (2023): October 2023 Vol 8, No 1 (2023): February 2023 Vol 7, No 3 (2022): October 2022 Vol 7, No 2 (2022): June 2022 Vol 7, No 1 (2022): February 2022 Vol 6, No 3 (2021): October 2021 Vol 6, No 2 (2021): June 2021 Vol 6, No 1 (2021): February 2021 Vol 5, No 3 (2020): October 2020 Vol 5, No 2 (2020): June 2020 Vol 5, No 1 (2020): February 2020 Vol 4, No 3 (2019): October 2019 Vol 4, No 2 (2019): June 2019 Vol 4, No 1 (2019): February 2019 Vol 4, No 1 (2019): February 2019 Vol 3, No 3 (2018): October 2018 Vol 3, No 2 (2018): June 2018 Vol 3, No 1 (2018): February 2018 Vol 3, No 1 (2018): February 2018 Vol 2, No 3 (2017): October 2017 Vol 2, No 2 (2017): June 2017 Vol 2, No 1 (2017): February 2017 Vol 2, No 1 (2017): February 2017 Vol 1, No 3 (2016): October 2016 Vol 1, No 2 (2016): June 2016 Vol 1, No 1 (2016): February 2016 Vol 1, No 1 (2016): February 2016 Vol 19, No 1 (2014): February 2014 Biota Volume 19 Nomor 1 Tahun 2014 Biota Volume 13 Nomor 2 Tahun 2014 Vol 18, No 2 (2013): June 2013 Vol 18, No 1 (2013): February 2013 Biota Volume 18 Nomor 1 Tahun 2013 Vol 17, No 3 (2012): October 2012 Vol 17, No 2 (2012): June 2012 Vol 17, No 1 (2012): February 2012 BIOTA Volume 17 Nomor 3 Tahun 2012 Vol 16, No 2 (2011): June 2011 Vol 16, No 2 (2011): June 2011 Vol 16, No 1 (2011): February 2011 Vol 16, No 1 (2011): February 2011 Vol 15, No 3 (2010): October 2010 Vol 15, No 2 (2010): June 2010 Vol 15, No 1 (2010): February 2010 Vol 14, No 3 (2009): October 2009 Vol 14, No 2 (2009): June 2009 Vol 14, No 1 (2009): February 2009 Vol 13, No 3 (2008): October 2008 Vol 13, No 2 (2008): June 2008 Vol 13, No 1 (2008): February 2008 Vol 12, No 3 (2007): October 2007 Vol 12, No 2 (2007): June 2007 Vol 12, No 1 (2007): February 2007 Vol 11, No 3 (2006): October 2006 Vol 11, No 2 (2006): June 2006 Vol 11, No 1 (2006): February 2006 Vol 10, No 3 (2005): October 2005 Vol 10, No 2 (2005): June 2005 Vol 10, No 1 (2005): February 2005 Vol 9, No 3 (2004): October 2004 Vol 9, No 2 (2004): June 2004 Vol 9, No 1 (2004): February 2004 Vol 8, No 3 (2003): October 2003 Vol 8, No 2 (2003): June 2003 Vol 8, No 1 (2003): February 2003 More Issue