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EKSTRAKSI MINYAK NILAM (Pogostemon Cablin BENTH) DENGAN TEKNIK HIDRODIFUSI PADA TEKANAN 1 â 3 BAR Harimurti, Niken; Soerawidjaja, Tatang H; Sumangat, Djajeng; Risfaheri, Risfaheri
Jurnal Pascapanen Pertanian Vol 9, No 1 (2012): Jurnal Pascapanen Pertanian
Publisher : Jurnal Pascapanen Pertanian

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ABSTRACT. Niken Harimurti, Tatang H Soerawidjaja, Djajeng Sumangat and Risfaheri. 2012. Patchouly oil extraction with hydrodiffusiontechnique at 1 â 3 bar pressure. Patchouli oil is a type of essential oil which was obtained by pressured steam or water distillation of Pogostemoncablin Benth dried leaves. Key components of patchouli oil are patchouli alcohol and norpatchoulenol which become indicators in determiningpatchouli oil quality. This research was objected to evaluate patchouli oil extraction with hydrodiffusion technique at 1-3 bar pressure. This experimentwas designed with response surface method, with pressure (Low level 1 bar, high level 3 bars) and time (low level 3 hours, high level 12 hours) asvariables. Yield, density, refractive index, optical polarization, solubility in ethanol 90%, acid number and ester number were evaluated as the responsof the two variables. Analysis of respons surface method showed that increasing extraction pressure significantly influence in acid number while theyield of patchouli oil was influenced by pressure, time and interaction of both. Results of qualitative analysis by GC / MS showed that oil samples atno 11 (pressure 3 bar for 3 hours) contained patchouli alcohol 29.66% and norpatchoulenol 0.68%. Microhistology identification of patchouli leavesbefore and after extraction showed reduction of glandular trichomas diameter about 61.2%. Based on quality parameters values, statistical and GC /MS analysis results, the best condition for hydrodiffusion process was at 3 bar pressure for 3 hours.Keywords: patchouli oil, hydrodiffusion, patchouli alcohol, norpatchoulenol

EKSTRAKSI MINYAK NILAM (Pogostemon Cablin BENTH) DENGAN TEKNIK HIDRODIFUSI PADA TEKANAN 1 â 3 BAR Harimurti, Niken .; Soerawidjaja, Tatang S.; Sumangat, Djajeng .; ., Risfaheri .
Jurnal Pascapanen Pertanian Vol 9, No 1 (2012): Jurnal Pascapanen Pertanian
Publisher : Jurnal Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar

ABSTRACT. Niken Harimurti, Tatang H Soerawidjaja, Djajeng Sumangat and Risfaheri. 2012. Patchouly oil extraction with hydrodiffusiontechnique at 1 â 3 bar pressure. Patchouli oil is a type of essential oil which was obtained by pressured steam or water distillation of Pogostemoncablin Benth dried leaves. Key components of patchouli oil are patchouli alcohol and norpatchoulenol which become indicators in determiningpatchouli oil quality. This research was objected to evaluate patchouli oil extraction with hydrodiffusion technique at 1-3 bar pressure. This experimentwas designed with response surface method, with pressure (Low level 1 bar, high level 3 bars) and time (low level 3 hours, high level 12 hours) asvariables. Yield, density, refractive index, optical polarization, solubility in ethanol 90%, acid number and ester number were evaluated as the responsof the two variables. Analysis of respons surface method showed that increasing extraction pressure significantly influence in acid number while theyield of patchouli oil was influenced by pressure, time and interaction of both. Results of qualitative analysis by GC / MS showed that oil samples atno 11 (pressure 3 bar for 3 hours) contained patchouli alcohol 29.66% and norpatchoulenol 0.68%. Microhistology identification of patchouli leavesbefore and after extraction showed reduction of glandular trichomas diameter about 61.2%. Based on quality parameters values, statistical and GC /MS analysis results, the best condition for hydrodiffusion process was at 3 bar pressure for 3 hours.Keywords: patchouli oil, hydrodiffusion, patchouli alcohol, norpatchoulenol

EKSTRAKSI MINYAK NILAM (Pogostemon Cablin BENTH) DENGAN TEKNIK HIDRODIFUSI PADA TEKANAN 1 â 3 BAR Harimurti, Niken; Soerawidjaja, Tatang H; Sumangat, Djajeng; Risfaheri, Risfaheri
Jurnal Pascapanen Pertanian Vol 9, No 1 (2012): Jurnal Pascapanen Pertanian
Publisher : Jurnal Pascapanen Pertanian

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ABSTRACT. Niken Harimurti, Tatang H Soerawidjaja, Djajeng Sumangat and Risfaheri. 2012. Patchouly oil extraction with hydrodiffusiontechnique at 1 â 3 bar pressure. Patchouli oil is a type of essential oil which was obtained by pressured steam or water distillation of Pogostemoncablin Benth dried leaves. Key components of patchouli oil are patchouli alcohol and norpatchoulenol which become indicators in determiningpatchouli oil quality. This research was objected to evaluate patchouli oil extraction with hydrodiffusion technique at 1-3 bar pressure. This experimentwas designed with response surface method, with pressure (Low level 1 bar, high level 3 bars) and time (low level 3 hours, high level 12 hours) asvariables. Yield, density, refractive index, optical polarization, solubility in ethanol 90%, acid number and ester number were evaluated as the responsof the two variables. Analysis of respons surface method showed that increasing extraction pressure significantly influence in acid number while theyield of patchouli oil was influenced by pressure, time and interaction of both. Results of qualitative analysis by GC / MS showed that oil samples atno 11 (pressure 3 bar for 3 hours) contained patchouli alcohol 29.66% and norpatchoulenol 0.68%. Microhistology identification of patchouli leavesbefore and after extraction showed reduction of glandular trichomas diameter about 61.2%. Based on quality parameters values, statistical and GC /MS analysis results, the best condition for hydrodiffusion process was at 3 bar pressure for 3 hours.Keywords: patchouli oil, hydrodiffusion, patchouli alcohol, norpatchoulenol

EKSTRAKSI MINYAK NILAM (Pogostemon Cablin BENTH) DENGAN TEKNIK HIDRODIFUSI PADA TEKANAN 1 â 3 BAR Harimurti, Niken .; Soerawidjaja, Tatang S.; Sumangat, Djajeng .; ., Risfaheri .
Jurnal Pascapanen Pertanian Vol 9, No 1 (2012): Jurnal Pascapanen Pertanian
Publisher : Jurnal Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar

ABSTRACT. Niken Harimurti, Tatang H Soerawidjaja, Djajeng Sumangat and Risfaheri. 2012. Patchouly oil extraction with hydrodiffusiontechnique at 1 â 3 bar pressure. Patchouli oil is a type of essential oil which was obtained by pressured steam or water distillation of Pogostemoncablin Benth dried leaves. Key components of patchouli oil are patchouli alcohol and norpatchoulenol which become indicators in determiningpatchouli oil quality. This research was objected to evaluate patchouli oil extraction with hydrodiffusion technique at 1-3 bar pressure. This experimentwas designed with response surface method, with pressure (Low level 1 bar, high level 3 bars) and time (low level 3 hours, high level 12 hours) asvariables. Yield, density, refractive index, optical polarization, solubility in ethanol 90%, acid number and ester number were evaluated as the responsof the two variables. Analysis of respons surface method showed that increasing extraction pressure significantly influence in acid number while theyield of patchouli oil was influenced by pressure, time and interaction of both. Results of qualitative analysis by GC / MS showed that oil samples atno 11 (pressure 3 bar for 3 hours) contained patchouli alcohol 29.66% and norpatchoulenol 0.68%. Microhistology identification of patchouli leavesbefore and after extraction showed reduction of glandular trichomas diameter about 61.2%. Based on quality parameters values, statistical and GC /MS analysis results, the best condition for hydrodiffusion process was at 3 bar pressure for 3 hours.Keywords: patchouli oil, hydrodiffusion, patchouli alcohol, norpatchoulenol

OPTIMASI PEMBUATAN MI DARI TEPUNG JAGUNG PRAGELATINISASI Nur Aini; Budi Sustriawan; nFN Masrukhi
Jurnal Penelitian Pascapanen Pertanian Vol 16, No 2 (2019): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpasca.v16n2.2019.99-109

Pragelatinisasi merupakan metode modifikasi yang dapat diterapkan untuk tepung jagung sehingga diharapkan dapat mempermudah pada proses pembuatan mi. Suhu gelatinisasi berbeda-beda bagi tiap jenis pati, dan untuk mencapai suju gelatinisasi tersebut tiap bahan memerlukan waktu yang berbeda. Setiap bahan juga membutuhkan penambhan air yang tepat agar dapat mengalam gelatinisasi. Penelitian ini bertujuan untuk (1) mempelajari kombinasi wkatu dan suhu pemasakan serta jumlah air yang ditambhakan untuk suspensi tepung jagung pragelatinisasi yang mempunyai respon maksimum terhadap kadar karbohidrat, kadar pengembangan dan tingkat penerimaan mi jagung; (2) mengetahui karakter fisiokimia dan organoleptik mi jagung pada formula optimal. Hasil penelitian menunjukkan bahwa ondisi pragelatinisasi optimal pada tepung jagung untuk pembuatan mi adalah selama pemasakan 7 menit waktu pemasakan, suhu 71C dan volume air 13% b/v. Mi jagung formula optimal memiliki kadar air 62,61%; kadar abu 0,322% bb; kadar lemak 1,37% bb; kadar protein total 4,18% bb; kadar karbohidrat 31,51% bb; daya pengembangan mi 16,67%; warna 2,5 9kuning keputihan); aroma 3,9 (tidak khas tepung); tekstur (2,5) (agak kenyal); rasa 2,6 (agak gurih); dan tingkat kesukaan keseluruhan 2,8 (suka). mi jagung ini masih memerlukan perbaikan proses untuk menurunkan kadar air yang tinggi, supaya memenuhi SNI 01-2987-1992. Pragelatinization is a modification method that can be applied for corn flour so that it can simplify the application for noodles. The temperature of gelatinization varies for each type of starch, and to reach the gelatinization temperature each ingredient needs different time. Each ingredient also needs the precise amount of water to get gelatinization. The object of this research are (1) to study the combination of cooking time and temperature and the amount of water needed for the suspension of pragelatinized corn flour which has a maximum response to carbohydrate levels, swelling power and preference of corn noodles; (2) to study the physicochemical and sensory characters of corn noodles in the optimal formula. The results showed that the optimal pregelatinization condition in corn flour for the production of noodles was during 7 minutes cooking time, temperature 71oC and volume of water 13% b / v. The optimal formula for corn noodles has a water content of 62.61%; ash content 0.322% bb; fat content 1.37% bb; total protein content 4.18% bb; carbohydrate levels 31.51% bb; power of development of noodles 16.67%; color 2.5 (whitish yellow); aroma 3,9 (not typical flour); texture 2.5 (slightly springy); flavor 2,6 (rather umami); and preference level 2.8. The corn noodles still need to improve the process to reduce the high water content so that they have not fulfilled Indonesian Nasional Standard 01-2987-1992.

RANCANGAN PROSES PRODUKSI FRUIT LEATHER BERBASIS PISANG SKALA USAHA KECIL MENENGAH (UKM) KAPASITAS 50KG/HARI Muhamad Kurniadi; Asep Nurhikmat; Annisa Kusumaningrum; Aldicky Faizal Amri; Dini Ariani
Jurnal Penelitian Pascapanen Pertanian Vol 16, No 2 (2019): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpasca.v16n2.2019.64-72

Penelitian ini bertujuan untuk membuat rancangan proses produksi fruit leather berbasis pisang skala UKM kapasitas 50 kg/hari. Metode penelitian yang digunakan pada tahap pertama adalah melakukan formulasi pisang dengan variasi jenis buah yaitu F1 (pisang /mangga), F2 (Pisang /nanas) dan F3 (pisang/sirsak) serta dilakukan pengujian kimia, mikrobiologi dan sensoris. Simulasi analisis kelayakan usaha dengan menggunakan kriteria kelayakan yaitu tingkat keuntungan, Benefit Cost Rasio (BCR ), IRR, NP, Break Event Point dan penentuan tata letak peralatan proses produksi. Data yang diperoleh disajikan dalam bentuk tabel, selanjutnya diolah dan dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa formula terpilih adalah F1 (pisang/mangga) mempunyai karakteristik kimia yang meliputi kadar air 18,00 %, vitamin C 115,80 mg/100g,serat pangan2,98%,pektin 12,80%, gula reduksi 12,65%, Aw 0,521 dan kuat tarik 6,37 N serta paling disukai panelis. Hasil simulasi kelayakan usaha fruit leather berbasis pisang skala UKM kapasitas 50 kg per hari layak dikembangkan, karena memenuhi kriteria kelayakan usaha yaitu : BCR 1,20; BEP (Rp) 35.111.358,57,-; BEP (unit) 5852, NPV 240.255.699,13, IRR 11,57% , rencana usaha produksi Fruit Leather lebih sensitif terhadap skenario penurunan pendapatan daripada kenaikan biaya. Tata letak peralatan produksi menggunakan bentuk U (U shape). Fruit Leather Production Process Design Based On Middle Small Scale Business Scale (UKM) Capacity 50 Kg/ DayThe aim of this research was to study about desain process of fruit leather production based on banana in home industry scale 50kg/day. The first step was formulated fruit leather based ono banana with varians of fruits i.e. F1 (banana.mango), F2 (banana/pineapple) and F3 (banana/soursop) then analyzed their characteristics (chemical, microbiology and sensory). Business properness analysis simulation used income level, Benefit Cost Ratio (BCR), IRR, NP, Break Event Point and production layout had been done. The data results presented in table and descriptive analyzed. The results showed that selected formula and most preferred and most by panelists was F1 (banana/mango) with water content 18%, vitamin C 115.8 mg/100g, fiber 2.98%, pectin 12.8%, reduction sugra 12.65%, water activity 0.521 and tensile strength of 6.37 N. Simulation results of business properness were BCR 1.20; BEP (Rp) 35.111.358,57; BEP (unit) 5852 NPV 240.255.699,13; IRR 11,57%. Layout of fruit leather production used U shape preferable.

Pengaruh Perlakuan pH dan Suhu Terhadap Sifat Fisikokimia Mocaf (Modified Cassava Flour) nurud diniyah; Pradiska Gita Vindy Ganesha; Achmad Subagio
Jurnal Penelitian Pascapanen Pertanian Vol 16, No 3 (2019): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpasca.v16n3.2019.147-158

Peningkatan sifat fungsional pati dapat dilakukan dengan mengubah struktur pati menjadi lebih banyak pada bagian amorf dengan cara perlakuan fermentasi pada umbi singkong terlebih dahulu sehingga dihasilkan Mocaf. Tujuan penelitian ini adalah untuk mengetahui perubahan karakteristik pati Mocaf dengan perlakuan panas dan pengaturan tingkat keasaman (pH) agar dapat digunaan dalam aplikasi yang lebih luas. Perlakuan dalam penelitian ini meliputi variasi suhu pemanasan (60, 70, 80, 90, dan 120 °C) dan pH (3, 4, 5, 6, dan 7) dan diulang sebanyak 3 kali dengan parameter pengukuran meliputi daya kembang, kelarutan, kekeruhan, viskositas pasta, bentuk granula pati, dan sineresis. Hasil penelitian ini menunjukkan bahwa suhu pemanasan mempengaruhi nilai daya kembang, kelarutan, viskositas, bentuk granula pati, dan sineresis Mocaf, tetapi menyebabkan penurunan tingkat kekeruhan. Sedangkan penambahan asam dapat mempengaruhi viskositas dan granula pati Mocaf. Effect of pH and Temperature Treatment on Physicochemical Properties of Mocaf (Modiffied Cassava Flour) The changes in the starch’s structure to amorphous can increase the functional characteristics of starch using the fermentation process of cassava to be known as Mocaf. The aim of this study was to determine the physicochemical properties of Mocaf’s starch due to temperature and pH processing. Treatment in this research including various of temperature (60, 70, 80, 90, and 120 °C) dan pH (3, 4, 5, 6, dan 7) in three replications with analysis of swelling power, solubility, viscosity, starch granule, and syneresis. The results of this study indicate that the heating temperature affects the value of swelling power, solubility, viscosity, starch granule, and syneresis of Mocaf, but it causes a decrease of Mocaf’s turbidity. The addition of acid may affect the viscosity and granules of Mocaf’s starch.

PENDUGAAN UMUR SIMPAN EKSTRAK KERING BEKU BIJI MELINJO KERIKIL (GNETUM GNEMON L. “KERIKIL”) BERDASARKAN KADAR SENYAWA RESVERATROL DAN AKTIVITAS ANTIOKSIDAN bambang kunarto; nFN Sutardi; nFN Supriyanto; Chairil Anwar
Jurnal Penelitian Pascapanen Pertanian Vol 16, No 2 (2019): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpasca.v16n2.2019.73-79

Biji melinjo kerikil mengandung resveratrol yang merupakan senyawa turunan stilbenoid dan mempunyai aktivitas antioksidan. Selama penyimpanan, senyawa resveratol dapat mengalami perubahan berupa oksidasi dan degradasi yang berdampak pada aktivitas antioksidannya. Penelitian ini bertujuan menduga umur simpan ekstrak kering beku biji melinjo kerikil berdasarkan kadar resveratrol dan aktivitas antioksidan (penghambatan radikal DPPH) sebagai indikator kerusakan. Pendugaan umur simapn dilakukan dengan metoda accelerated shelf life test (ASLT). Metoda ini didasarkan pada penyimpanan pada kondisi yang direkayasa sehingga mempercepat yang terjadinya kerusakan. Penyimpanan dilakukan selama 35 hari pada suhu simpan 35°C, 45°C adn 55°C. Hasil penelitian ini menunjukkan bahwa parameter mutu kritis pada penyimpanan ekstrak kering beku melinjo kerikil adalah aktivitas antioksidan (penghambatan radikal DPPH) karena memiliki energi aktivasi (1301,09 kal/mol) yang lebih rendah dibandingkan energi aktivasi kadar senyawa recveratrol. Umur simpan ekstrak keirng beku biji melinjo kerikil pada penyimpanan 35; 45; 55 dan 28°C berturut-turut adalah 13,57 hari, 12,67 hari, 11,83 hari dan 14,56 hari. Shelf life estimation of melinjo kerikil seeds freeze-dried extract based on resveratrol and antioxidant activityMelinjo kerikil seeds contain resveratrol which is a stilbenoid derivative compound and has antioxidant activity. During storage, resveratrol can be dagraded which have an impact on their antioxidant activity. The aim of this study wa to estimate teh shelf life of melinjo kerikil seed freeze-dried extract based on resveratrol content and inhibition of DPPH radicals as an indicator of damage. Estimation of shelf life is carried out by the accelerated shelf life test (ASLT). This method is based on storage in conditions that are engineered so as to speed up the occurrence of damage. Storage is carried out for 35 days at the temperature of 35°C, 45°C, and 55°C. The results showed that the critical quality parameter in the storage of melinjo kerikil seed freeze-dried extracs was inhibition of DPPH radicals. This is because the activation energy of inhibition of DPPH radicals is lower than the revertrol activation energy. The Shelf life of melinjo kerikil seed freeze-dried extract at storage 35; 45; 55 and 28°C respectively were 13.57 days, 12.67 days, 11.83 days and 14.56 days.

Perubahan Sifat Fisikokimia dan Fungsional Tepung Beras Akibat Proses Modiflkasi Ikat-Silang S. Joni Munarso; D. Muchtadi; D. Fardiaz; R. Syarief
Jurnal Penelitian Pascapanen Pertanian Vol 1, No 1 (2004): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpasca.v1n1.2004.22-28

Penelitian ini dilakukan untuk mengevaluasi perubahan sifat fisik, kimia dan fungsional tepung beras akibat proses modifikasi ikat-silang. Sebanyak 4 jenis beras, yaitu IR64B, IR64J, IR42B, dan IR42J yang beragam dalam hal mutu dan kadar amilosanya direaksikan dengan fosfor-oksiklorida (POCI,) dengan konsentrasi yang bervariasi, yi. 0, I; 0,2 dan 0,3% pada pH 10,5. Hasil penelitian menunjukkan bahwa tepung beras terfosforilasi atau terikat-silang terbukti mengalami perubahan, yakni kenaikan derajad putih, dan berbagai perubahan sifat kimia maupun fungsional lain. Kadar pati tepung beras makin menurun akibat penggunaan POCI3 dengan konsentrasi yang makin tinggi. Akibat penurunan kadar pati ini, kadar komponen kimia lain, seperti protein, lemak, serat, abu dan fosfor mengalami peningkatan. Pada tepung beras terikat-silang juga diperoleh sifat pasta yang meningkat baik pada viskositas puncak, viskositas pasta, maupun viskositas balik. Changes of physicochemical and functional properties of rice flour as affected by cross link modification processAn experiment to evaluate the changes of physical, chemical and functional properties of rice flour as affected by cross-linked modification process has been executed. Four rice samples, namely IR64B. IR64J, IR42B. and lR42J, which differrent quality and amylose content, were reacted with phosphor-oxychloride (POCl,) at various concentrations, i.e. 0.1 ; 0.2 and 0.3%, at the pH of 10.5. The result shows that phosphorilated or cross-linked rice flour had been identified to have higher whiteness degree. and some' changes in chemical and functional properties. Starch content of rice decreased as higher concentration of POCl, is applied. Meanwhile, the content of other chemical compounds, such as protein, fat, fiber, ash and phosphorous, increased as starch content decreased. Higher peak, paste, and set back viscosities were also observed in cross-linked rice flour.

PRODUKSI ENZIM SELULASE OLEH AKTINOMISET MENGGUNAKAN FROND SAGU Sumarni Nompo; Anja Meryandini; Titi Candra Sunarti
Jurnal Penelitian Pascapanen Pertanian Vol 16, No 2 (2019): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpasca.v16n2.2019.80-89

Fround Sagu adalah pucuk batang sagu yang masih dibungkus oleh pelepah dan tidak diamnfaatkan oleh industri pengolahan sagu. Fround sagu memiliki kandungan serat dengan kandungan selulosa yang tinggi serta berpotensi dijadikan bahan baku untuk produksi selulase. Enzim selulase diproduksi melalui kultivasi substrat cair frond sagu oleh Aktinomiset. Subtart berupa tepung sagu dan ampas frond sagu, diinokulasi oleh isolat HJ4 (3b) dan HJ5 (4b). Kedua isolat diremajakan dalam medium ISP-4 selama 5 hari, kemudian diinokulasikan ke dalam media tepung frond sagu dan ampas frond dan diinkubasi dalam shaker pada suhu runag selama 9 hari. Kedua isolat Aktinomiset mampu menghasilkan enzim selulase pada kedua substrat dan metode kultivasi. Isolat HJ4 (3b) dan HJ4 (5b) pada perlakuan kultivasi substrat padat ampas frond sagu menghasilkan aktivitas spesifik yaitu endoglukase (CMCase) tertinggi yaitu 0.314 U mg-1 dan 0.294 U mg-1 dan aktivitas spesifik enzim eksoglukanase (FPase) yaitu 0.269 U mg-1 dan 0.258 U mg-1, sedangkan pada perlakuan kultivasi substat padat menggunakan tepung frond sagu dihasilkan aktivitas spesifik endoglukanase masing-masing sebesar 0.258 U mg-1 dan 0.254 U mg-1 serta aktivitas spesifik eksoglukanase 0.205 U mg-1 dan 0.198 U mg-1. Production of Cellulase Enzyme by Actinomycet Using Sago FrondSago frond is the upper part of sago trunk which is still wrapped by leaflet, and is not used by the sago processing industry. Sago frond contains fiber with high cellulose content that could potentially be used by as raw material for cellulase production. Cellulase enzymes were produced through both solid-state and submerged cultivation of sago frond by Actinomicycetes. Two substrates, sago frond flour and pulp of sago fronds, were inoculated by isolate HJ4 (3b) and HJ4 (5b). Both isolates were rejuvenated in Sp-4 medium for 5 days, then were inoculated into substrate of frond flour and hampas, and were incubated in a shaker at room temperature for 9 days. Both Actinomycetes isolates were able to produce cellulase enzymes by using both substrates and cultivation methods. The isolates of HJ4 (3b) and HJ4 (5b) by using pulp and solid-state cultivation produced the highest endoglucanase (CMCase) specific activity of 0.294 U mg-1 and 0.276 U mg-1 and exoglucanase (FPase) substrate specific activity os 0.252 U mg-1 and 0.241 U mg-1, while in the solid-state cultivation and by using sago fronds flour resulted in specific endoglucanase activities which were 0.242 U mg-1 and 0.238 U mg-1 and exoglucanase specific activities 0.192 U mg-1 and 0.185 U mg-1, respectively.

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