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Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
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Articles 13 Documents
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Search results for , issue "Vol. 2 No. 3 (2012)" : 13 Documents clear
The Growth of Lactobacillus fermentum Isolated from Quail Intestine on Rice Bran Medium Umi Kalsum; O. Sjofjan
Journal of Tropical Life Science Vol. 2 No. 3 (2012)
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The objective of this study was to investigate the growth of Lactobacillus fermentum isolated from the intestine of Japanese quail (Coturnix japonica) on medium variation of rice bran meal. The influence of this medium preparation on the cell number and produce of lactic acid of L.fermentum was evaluated every six hour in 36-hour incubation. A randomized block design was used to assign three variation of rice bran medium (6, 9 and 12) %. The result showed that the higest cell number was in 9 % rice bran medium at pH 4.7 at 12th hour was 5.2 x 1010 CFU/ml and the highest lactic acid concentrations were 0.57 %. The fastest speed of growth in 9 % rice bran medium was 0.92 generation/hour and generation time 45 minutes. In conclusion, the optimum growth of L.fermentum was in 9 % rice bran medium at 12th hour.Keywords: Lactobacillus fermentum, growth, quail, rice bran.
Binding Inhibition Between Igf1r and Igf1 by Catechin of Black Tea Lina Firdausi; M Rasjad Indra; fatchiyah .
Journal of Tropical Life Science Vol. 2 No. 3 (2012)
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The natural compound of black tea is used as an alternative of obesity therapies in the world; particularly, the catechin family in tea leaves which has bioactive compounds such as EC, EGC and EGCG. Their bioactivity contributes to inhibit the ligand of Insulin-Like Growth Factor I Receptor (Igf-1r) binding-region to Igf-1 protein. To elucidate the inhibiton of Igf-1 expression and proliferating of Rattus norvegicus strain wistar adipose cell using black tea solution. The research used Rattus norvegicus strain wistar. After a 90-day treatment, the adipose tissues were picked up from the viscera of each experimental animal, and then the adipose tissues were embedded by paraffin. The paraffin sections were determined through immunohistochemistry with anti-Igf-1 antiserum, and were also analyzed through hematoxylin-eosin. A protein sequence of Igf-1, Igf-1r, and 3D structure of EC, EGC and EGCG from Gene Bank sites were used during in silico analysis. The sequences were aligned by BLAST program to identify the conserve and variable domain of IGF-1 protein isoforms. The 3D structures of IGF-1 and IGF-1R were constructed using Phyre program. The ligand among the 3D structures of IGF-1, IGF-1R and catechin compounds were analyzed using Hex 5.1 docking program. The data showed that the Igf-1 expression of adipose cells was reduced at 0,03 g/ml BTS and 0,045 g/ml BTS treatments. The result of BLAST analysis showed that IGF-1 (a, b, c, and d) isoforms conserved a domain from amino acid no 22 until 134; and this region was a variable region. The EGCG bound L1 domain of IGF-1R with E-total -235.3 KJ/mol which was lower than EC (-208,4 KJ/mol) and EGC (-142 KJ/mol). The total energy of IGF-1 (a, b, c, but not d isoform) which interacted with EGCG was around -223.7 KJ/mol, EC is -205.6 KJ/mol and EGC was -191.7 KJ/mol. However, EC, EGC and EGCG was only able to prevent the interaction between the L1 of IGF-1R with IGF-1 protein, but not the opposite.Keywords: Adipose cell, black-tea, proliferation, catechin, IGF-1, IGF-1R, inhibition, docking
The Increase of Arthropods Biodiversity in Paddy Field Ecosystem Managed by Using Integrated Pest Management at South Borneo Samharinto Samharinto; Abdul Latief Abadi; Bambang Tri Raharjo; Hakimah Halim
Journal of Tropical Life Science Vol. 2 No. 3 (2012)
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We have studied the arthropods biodiversity in two paddy field ecosystems, namely, paddy field ecosystem using Integrated Pest Management (IPM) system and non-IPM paddy field ecosystem. This study was conducted from April 2011 - November 2011 in three locations, that is, Pasar Kamis village and Sungai Rangas village in Banjar regency, and Guntung Payung village in Banjarbaru city, South Borneo Province. In this study, we used insect nets, yellow sticky traps, light trap and pitfall trap to get the sample or catch the arthropods in one period of planting season. The arthropods caught were then classified into some classes: pest (herbivore), natural enemy (parasitoid and predator), and other arthropods. After that, the Species Diversity Index was determined using its Shannon-Wiener Index (H), Evenness (e), Species Richness (R), and Species Similarity Index (IS). The sum of arthropods which have the characteristic of pest and parasitoid were higher in the IPM paddy fields than in the non-IPM paddy fields, and the sum of other arthropods were the same. The highest H and e values were in the IPM paddy field in Pasar Kamis village. The IS value for each three locations were 77.5% in Pasar Kamis village, 93.42% in Guntung Payung village, and 78.76% in Sungai Rangas village.Keywords: diversity, arthropods, ecosystem, and SLPHT
Growth Adaptation of Two Indonesian Black Rice Origin NTT Cultivating In Organic Paddy Field, Malang-East Java Budiman Budiman; Endang Arisoesiloningsih; Ratno Bagus Edi Wibowo
Journal of Tropical Life Science Vol. 2 No. 3 (2012)
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This research is aimed to study the growth adaptation of two Indonesian black rice NTT in origin which is cultivating in organic paddy field in Malang - East Java and its problems during a planting period. A descriptive exploratory research was conducted in two organic rice fields in Kepanjen District, Malang Regency. Those fields were grown with two different varieties of black rice NTT in origin namely, Laka and Woja Laka. Each rice field was recorded periodically. The growth was including the plant height, biomass, density of tiller, panicle and spikes, dry seed weight and the problem correlated to pest and climates limitation. The results showed that Woja Laka and Laka black rice varieties slightly gave a different growth pattern. Laka variety was taller than Woja Laka, i.e. the Laka could reach 200 cm of height, while the Woja Laka was maximum 150 cm of height. However, the density of Woja Laka tiller and its panicle were almost double than the Laka ones. The dry seed weight and spikes number of Woja Laka were much higher than in Laka ones. In terms of organic farming system, Laka rice seemed to be more sensitive towards the strong wind conditions and bird or rat attacks rather than Woja Laka. Both pests attacked on both black rice along growing period, but rat preferred to attack vegetative part, otherwise bird strongly disturbed during generative period. Key words: black rice, growth, Laka, Woja Laka, organic paddy field
Expression of Insoluble Influenza Neuraminidase Type 1 (NA1) Protein in Tobacco Teen Lee Pua; Hwei San Loh; Festo Massawe; Chon Seng Tan; Abdul Rahman Omar
Journal of Tropical Life Science Vol. 2 No. 3 (2012)
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The avian influenza virus, particularly H5N1 strain, is highly virulent to poultry and mankind. Several expression systems, like yeast, baculovirus and mammalian cells, have been adopted to produce vaccine candidate for this lethal disease. The present research aimed at developing a recombinant vaccine candidate, neuraminidase type 1 (NA1), for the Malaysia isolate of H5N1 in Nicotiana benthamiana. The NA1 gene was fused directly in-frame in cowpea mosaic virus (CPMV)-based pEAQ-HT vector with C-terminal polyhistidine-tag incorporated to ease the subsequent purification step. The expression of the NA1 gene in tobacco was confirmed at RNA and protein levels at 6 days post-infiltration (Dpi). From the insoluble fraction of the protein, a recombinant glycosylated NA1 protein with a molecular weight of ~56 kDa was immunogenically detected by a specific anti-NA polyclonal antibody. We report for the first time the insolubility of the plant-made NA1 protein where a native sequence was used for its expression. This study signifies the necessity of the use of optimised sequences for expression work and provides great opportunity for the exploration of plant-manufactured NA1 protein as vaccine candidate.
Earthworm Population Density in Sugarcane Cropping System Applied with Various Quality of Organic Matter Nurhidayati Nurhidayati; Endang Arisoesilaningsih; Didik Suprayogo; Kurniatun Hairiah
Journal of Tropical Life Science Vol. 2 No. 3 (2012)
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Earthworms population in the soil are greatly impacted by agricultural management, yet little is known about how the quality and quantity of organic matter addition interact in sugarcane cropping system to earthworm population. This study describes the effect of various organic matter and application rates on earthworms in sugarcane cropping system. Earthworms were collected in April, July and December from 48 experimental plots under five kinds of organic matter application : (1) cattle manure, (2) filter cake of sugar mill, (3) sugarcane trash, (4) mixture of cattle manure+filter cake, and (5) mixture of cattle manure+sugarcane trash. There were three application rates of the organic matter (5, 10, and 15 ton ha-1). The treatments were arranged in factorial block randomize design with three replications and one treatment as a control (no organic input). Earthworms were collected using monolith sampling methods and hand-sorted from each plot, and measured its density (D) (indiv.m-2), biomass (B) (g m-2) and B/D ratio (g/indiv.). All the plots receiving organic matter input had higher earthworm density, biomass, and B/D ratio than the control. The highest earthworm population density was found in the plot receiving application of sugarcane trash (78 indiv.m-2) and the mixture of cattle manure+sugarcane trash (84 indiv.m-2). The increase in application rates of organic matter could increase the earthworm density and biomass. Earthworm population density also appeared to be strongly influenced by the quality of organic matter, such as the C-organic, N, C/N ratio, lignin, polyphenols, and cellulose content. Earthworm preferred low quality organic matter. It was caused by the higher energy of low quality organic matter than high quality organic matter. Our findings suggest that the input of low quality organic matter with application rate as 10 ton ha-1 is important for maintaining earthworm population and soil health in sugarcane land.Keywords: earthworms, organic matter quantity, quality, sugarcane cropping system
Embryo Development of Tree Frog Polypedates leucomystax at Campus of State University of Malang Pearlindah Peralindah; Emy Kusumawati; Dian Ratri Wulandari; Dwi Listyorini
Journal of Tropical Life Science Vol. 2 No. 3 (2012)
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Tree frogs live in natural places which are unpolluted. Regarding their role as an ecological indicator, the decrease of frogs population in a particular habitat indicates the danger of environment quality decrease. Moreover, this condition can harm the frogs themselves. All kinds of frogs breed in aqueous environment such as ponds, marshes, and farming fields. One of the tree frogs, Polypedates leucomystax, which belongs to Familia Rachophoridae, is widely spread in Indonesia. This frog has yellowish brown skin with black spots or six lines extending from head to the posterior tip of body. A breeding couple of the frog produces foam nests on the water or plants around water body, where they will nest their fertilized eggs. This species produces over a hundred embryos in one spawning season. These embryos require appropriate conditions to develop normally in the nature. Frog embryo development may becomes a reference to understand how the frog population survives. This study focused on P. leucomystax with regards to its decrease in number due to the drying up of the environment and a lot lost of trees in Campus of State University of Malang. The development of P. leucomystax embryos in the reproduction foam was observed until it reached a tadpole stage. The result showed that the embryos developed in the foam until they hatched then they move out of the foam into the water under which they would continue their development. Considering that water body is a critical requirement for the development of P. leucomystax embryos, it is our responsibility to make any efforts to conserve not only the trees but also any type of water bodies including ponds, marshes, and farming fields as well. Keywords: embryo development, Polypedates leucomystax, Tree frog conservation
Composition of Trees Grown Surrounding Water Springs at Two Areas in Purwosari Pasuruan, East Java soejono soejono
Journal of Tropical Life Science Vol. 2 No. 3 (2012)
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The aim of the research was to find out the composition of trees grown surrounding water springs at two areas in Purwosari, Pasuruan, East Java. Eleven plots for each area were observed. The data were analyzed using Mueller-Domboiss method to calculate their importance value indexes, while Shannon-Wieners formula was used for determining the diversity index. The coordinate and altitude of every water spring or its groups site was determined using Geographical Position System (GPS) to know their positions on the map. The result indicated that there were at least 30 families, 49 genera which consisted of 68 species of trees grown surrounding water springs at the first area with 5.49 of diversity index, while the second area, consisted of 34 families, 63 genera and 79 species of trees with 5.24 diversity index. The diversity of trees species from Moraceae was the highest among other families, both at the first and the second area, whereas, trees species having a significant important value index included Bambusa blumeana, Dendrocalamus asper, Ficus racemosa, Horsfieldia irya and Ficus virens. The position of the springs in the two areas within the sub-districts of Purwosari is in the range of 7º44'448 " south latitude; 112º44'353" east longitude up to 7º46'339 " south latitude; 112º41190" east longitude at an altitude between 251 and 522 m above sea level. We hope that the information can be used as an alternative guidance to restore or to conserve such areas in order to save and sustain trees diversity and their habitat.Keywords: composition, trees, water springs, Purwosari, Pasuruan.
Description Of Avian Bio-Diversity Of Damdamma Jheel In Gurgaon District In Haryana, India Rohtash Chand Gupta; Tirshem Kumar Kaushik
Journal of Tropical Life Science Vol. 2 No. 3 (2012)
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Damdamma Jheel is a wetland of impressive potentials of harboring rich avian bio-diversity in the accompaniment of enhanced habitat reconstruction and improvement. It is located in the National Capital Region, nearby Sohana town in Gurgaon district in Haryana merely 45 Kms from Dhaula Kuan in New Delhi. At present, compared to Sultanpur National Park, it is confronting neglect. Considering its positive features of terrain, water sheet, it has the qualities of being a rich habitat for birds of all hues including the winter migratory birds. The present studies focus attention on its avian biodiversity as well as its own degraded form and structure. In all, 128 species of birds belonging to 15 orders and 41 families could be enlisted. Order Passeriformes is the predominant one having 54 species, followed by Charadriiformes (14 Species) and Ciconiiformes (12 Species). The prominent winter migratory birds observed from Damdamma Jheel include Asian Openbill Stork Anastomus oscitans, Eurasian Spoonbill Platalea leucorodia, Northern Shoveller Anas clypeata, Northern Pintail Anas acuta, Common Teal Anas crecca, Common Pochard Aythya ferina, Gadwall Anas strepera, Spot-billed Duck Anas poecilorhyncha, Spotted Redshank Tringa erythropus, Common Redshank Tringa tetanus, Common Greenshank Tringa nebularia, Kentish Plover Charadrius alexandrinus, White-tailed Lapwing Vanellus leucurus, Marsh Sandpiper Tringa stagnatilis, Wood Sandpiper Tringa glareola, Common Sandpiper Actitis hypoleucos, White Wagtail Motacilla alba and Yellow Wagtail Motacilla flava. The lake is suffering from water scarcity considering its capacity and potential to have more water. The periphery towards east is plagued by the growth of peats. The very fact that in October 2012, a large group of Openbill Stork (Nearly 70-80) has already arrived at Damdamma Lake since September-2012 indicates that it is the largest HARBOUR of Open-bill Stork in Haryana. Also, Eurasian Spoonbills were seen in a group 10-12, again the largest in Haryana. It is argued in this paper, that Damdamma is the pride lake of Haryana and prime harbor for uncommon winter migratory birds like Open-bill Stork and Eurasian Spoonbill must be the prime place for habitat reconstruction by Ministry of Environment and Forest, Govt. of India in conjunction with Govt. of Haryana with the active contributions by WWF (India) to add to the might vastness of this lake an element of grace and glory so as to conserve the winter migratory birds during their sojourn in India. In addition, it will be one nodal point to include Sultanpur National Park, Badkhal Lake, Bhindawas and Keoladeo National Park as an eco-tourism rectangle with provision for night halt.
Genetic Diversity Study Among Six Genera of Amaranth Family Found in Malang Based on RAPD Marker Arik Arubil Fatinah; Estri Laras Arumingtyas; Retno Mastuti
Journal of Tropical Life Science Vol. 2 No. 3 (2012)
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Genera of amaranth family tend to have phenotypic variation partly caused by environmental factor. Phenotypic variation was the result of interaction between genetic and environmental factors. One of molecular markers that is widely used for detecting genetic variation is RAPD. RAPD is used for polymorphism detections and is now possible for identifiying a large number of loci and ascribes unambiguous taxonomic and genetic relationships among different taxa. Members of amaranth family found in Indonesia are Amaranthus, Celosia, Aerva, Alternanthera, Achyranthes, Gomphrena, Salsola, and Iresine. Six genera of which (Amaranthus, Celosia, Aerva, Alternanthera, Achyranthes, and Gomphrena) were observed in this study. DNA was extracted from fresh young leaves using Doyle and Doyles method with modification in the extraction buffer used. RAPD analyses were carried out with 20 decamer primers from Kit A of Operon Technology. DNA was amplified using master cycler gradient Eppendorf with 35 cycles. RAPD products were separated on 1,5 % agarose gels and detected by staining with ethidium bromide. There were 374 bands generated in 18 random primers. The number of monomorphic bands, polymorphic bands, and the percentage of polymorphism were 21 bands, 353 bands, and 94,38 % respectively. The high number and percentage of polymorphic bands revealed genomic DNA variation. This variation is in accordance with phenotypic variation detected in this experiment. Therefore, it can be concluded that, based on DNA polymorphism detected by RAPD, Amaranth family can be classified into two sub families namely Amaranthoideae and Gomphrenoideae.

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