cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kota malang,
Jawa timur
INDONESIA
Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences
Published by Universitas Brawijaya
ISSN : 20875517     EISSN : 25274376     DOI : -
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
The Journal of Tropical Life Science (JTLS) provides publication of full-length papers, short communication and review articles describing of new finding or theory in living system, cells and molecular level in tropical life science and related areas. The journal publishes articles that report novel findings of wide Tropical Life system phenomenon in the areas of biodiversity, agriculture, fisheries, health, husbandry, forestry and environmental technology. JTLS has 1 volume with 3 issues per year.
Arjuna Subject : -
Articles 815 Documents
 70   71   72   73   74 
In silico Characterization of UGT74G1 Protein in Stevia rebaudiana Bertoni Accession MS007 Afiqah Rahmatullah Khan; Nor Iwani Mokhtar; Zarina Zainuddin; Nurul Hidayah Samsulrizal
Journal of Tropical Life Science Vol. 11 No. 3 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.03.09

Abstract

Stevia rebaudiana is being promoted as an alternative sweetener in particular for diabetic and obese patients due to its low-calorie property. The steady demand in the market for high-quality stevia extracts presents a challenge for enhanced production of steviol glycosides that are safe for human consumption. This study characterized the structure and content of gene involved in the production of UGT74G1 protein for S. rebaudiana accession MS007 through in silico analysis using transcriptome dataset of stevia MS007.  Homologous search using BLASTp show high similarity to Q6VAA6 RecName: Full=UDP-glycosyltransferase 74G1 (S. rebaudiana) as the top hit sequences. InterPro family and domain protein motif search revealed the presence and entry of IPR002213 and IPR035595. The construction of the phylogenetic tree was done by selecting 19 out of 102 protein sequences from BLASTp. The phylogenetic analysis showed the same protein family which is Asteraceae. ProtParam Ex-Pasy, PSIPRED and Phyre2 computed the primary, secondary, and tertiary structures for UGT74G1 protein. The UGT74G1 predicted tertiary structure scored 100.0% confidence by the single highest scoring template and coverage of 96%. The model has dimensions (Å) of X: 57.609, Y: 70.386, and Z: 58.351. Outcomes of this research will help to enhance the understanding of UDP-glycosyltransferase 74G1 (S. rebaudiana MS007) characteristic and enhance target identification processes to improve understanding of protein-protein interaction in S. rebaudiana MS007.
The expression of OsPLA2-III and OsPPO genes in rice (Oryza sativa L.) under Fe toxicity stress . Turhadi; . Hamim; Munif Ghulamahdi; . Miftahudin
Journal of Tropical Life Science Vol. 11 No. 2 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Lipid is an important biomolecule in plant because of their structural and functional roles in plant cells. Moreover, lipid could be a signal molecule in defense system of plants under biotic and abiotic stresses. Detoxification process of toxic substances, which is one of the defense system and controlled by various genes, plays an important role in tolerance mechanism in plant under stress conditions, including iron (Fe) toxicity. Plants develop various tolerance strategies to cope the Fe toxicity. The objective of this research was to investigate the expression of OsPLA2-III and OsPPO genes under Fe stress condition. Two-weeks-old seedlings of two rice varieties, i.e., IR64 (Fe-sensitive toxicity) and Pokkali (Fe-tolerant toxicity) were used. Both varieties were treated with 1 (control) and 400 ppm of Fe in the form of FeSO4.7H2O in the nutrient culture solution. The leaf bronzing, chlorophyll content, and relative expression of OsPLA2-III and OsPPO genes were observed. An in-silico study was also performed to predict the interaction between OsPLA2-III and OsPPO proteins. The results showed that the Fe toxicity induced leaf bronzing, reduced leaf chlorophyll content, and increased the OsPLA2-III and OsPPO gene expression levels. Both genes are suggested to have a role in plant tolerance mechanism during Fe toxicity stress via lipid signaling pathway.
A Review on Enzymatic Response to Salt Stress and Genomic/Metagenomic Analysis of Adaptation Protein in Hypersaline Environment Habeebat Adekilekun Oyewusi; Muhammad Muhammad; Roswanira Abdul Wahab; Fahrul Huyop
Journal of Tropical Life Science Vol. 11 No. 3 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.03.11

Abstract

Microorganisms adapted to conditions of high salinity (low water activity) provide an understanding on how the problem of maintaining an efficient cell integrity under high osmotic stress conditions that had been tackled naturally. Almost all microbes adapting to extreme situations either by intracellularly amass inorganic ions (K+) to counterbalance high salt concentration or by synthesizing and accumulating certain organic solutes called compatible solutes that confer protection without affecting cell functions and this process may be chloride ion dependent in some microorganisms. However, the use of culture-independent method like genomic or metagenomics shields more light on the microbial diversity, gene structure and regulation as well as discovery of novel genes that led to understanding of their adaptation mechanism and roles in extreme environments. Therefore, microbes that survive this natural attenuation aimed at acclimatizing with the extreme environments could serve as the sources of biotechnologically essential molecules with an extensive array of uses.
Clinical and Laboratory Features of COVID-19 in Ulin Referral Hospital of South Kalimantan: Predictors of Clinical Outcome Haryati Haryati; Mohamad Isa; Ali Assagaf; Ira Nurrasyidah; Erna Kusumawardhani; Eko Suhartono; Fidya Rahmadhany Arganita
Journal of Tropical Life Science Vol. 11 No. 3 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.03.06

Abstract

Corona Virus Disease (COVID-19) is becoming a global pandemic. Indonesia, especially South Kalimantan had recorded increasing cases with a high fatality rate of 3.7%. Information about factors related to outcomes based on clinical and laboratory features in Indonesia is still limited. Identification of the risk is crucial to determine optimal management and reducing mortality. This retrospective study enrolled 455 adults COVID-19 patients, and data were extracted from medical records of Ulin General Hospital Banjarmasin. The latter is COVID-19 referral hospital in South Kalimantan between March-November 2020. Demographic data, comorbidities, and laboratory were all collected. Data were compared between survivors and non-survivors. Fisher’s exact test and chi-square were used to compare categorical variables. The Mann_Whitney U test was used to compare continuous variables. Analysis was continued by multivariate logistic regression then receiver operating characteristic (ROC) curve to determine cut-off value. The multivariate analysis showed that number of comorbidities [odds ratio (OR) 1,339 (95% confidence interval (CI): 1,064-1,685, P = 0,013) was a significant risk factor to the outcome. In laboratory, lactate dehydrogenase (LDH) [OR: 1.001, 95% CI: 1,000-1.002, P = 0.001], Ferritin (OR 1.000, CI: 1,000-1.001, P = 0.013), APTT (OR: 1.045, CI: 1.010-1.082, P = 0.012), and D-dimer (OR: 1.188, CI: 1.064 - 1.327, P = 0.002) were significant predictor factors but only LDH, ferritin, and D- dimer were obtained good AUC 0.731, 0.715, and 0.705, respectively. The cut of the value of LDH was 656.5 U/L, ferritin was 672.18 ng/ml, and D-dimer was 2.28 mg/L. Sensitivity and specificity were 66.7% and 68,0% for LDH, 83,2% and 56,3% for ferritin, and 62,8 and 70,8% for D-dimer. From this research, we revealed that the number of comorbidities was a risk factor for death. Elevated LDH, ferritin, and D-dimer could be good predictive factors for poor outcomes, thereby considering the accelerating management of COVID-19 patients.
Species Composition and Assessment of Sphingidae in Mt. Agad-Agad, Iligan City, Philippines Romeo Jr. Rogano Patano; Alma B Mohagan; Maria Melanie M Guiang; Olga M Nuñeza; Fulgent P Coritico; Honey Christine T Anches; Hanah Gloricel H Jebulan; Erl Pfian T Maglangit; Victor B Amoroso
Journal of Tropical Life Science Vol. 12 No. 1 (2022)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.12.01.14

Abstract

Sphingidae (Hawkmoth or Sphinxmoth) is a known bioindicator that is naturally affected by environmental issues such as deforestation and pollution. Members of thisfamily are widely distributed in Southeast Asia and play vital roles as indicators ofhabitat quality and biomass degradation. This study highlights the diversity and assessment of hawkmoths of Mt. Agad-Agad (Sitio Langinlanon, Barangay Puga-an)on Iligan City. Two sampling stations were established, the upper and lower Mt.Agad-Agad. Light trap sampling using 2 × 3 m2 white silk cloth and 500 watts lightbulb was established in selected points of the sampling stations. Six species belonging to four genera were recorded of which two species, Acosmeryx socrates and Polyptychus trilineatus philippinensis, were found very rare in the area. Station 2 (lowerMt. Agad-Agad) had the highest number of species recorded compared to Station 1(upper Mt. Agad-Agad). However, there is no significant difference between the two(t=0.59, critical t=2.2281, p=0.56) sampling stations in terms of species composition. Moreover, two species from the genus Ambulyx are the endemic species observed, viz, A. bakeri and A. wilemani.
Protein Profiling of Orthosiphon Stamineus Embryogenic Callus Using a Proteomic Approach: Protein Profiles of Orthosiphon stamineus Embryogenic Callus Dayangku Dalilah Mamat; Zaidah Rahmat; Tsun-Thai Chai; Fazilah Abd Manan
Journal of Tropical Life Science Vol. 12 No. 2 (2022)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.12.02.01

Abstract

Orthosiphon stamineus is one of the most prominent medicinal herbs widely grown in Southeast Asia. Propagation of O. stamineus using tissue culture technique helps to rapidly produce samples for research purposes, increase the production of secondary metabolites and is considered as one of the strategies in plant genetic improvement. Despite vast information on O. stamineus pharmacological properties, the protein profile of this species is currently underexplored. In the present study, proteins expressed in embryogenic callus developed from O. stamineus leaves in Murashige and Skoog medium supplemented with 1-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D) were identified via Liquid chromatography- tandem mass spectrometry (LC-MS/MS). The presence of embryogenic callus was confirmed with microscope observation and Evan Blue assay. Using this proteomic approach, we identified peptides that represent 22 proteins localized to different cell compartments and involved in various biological and metabolic functions in plants. This finding is useful to improve our understanding of protein functions, thus their future applications could be further explored   Keywords: Callus, Embryogenic, Herb, Orthosiphon stamineus,Proteins
Immunomodulatory Activity of Methanol Leaf Extract of Neem (Azadirachta indica Juss) Against Suppressor and Proinflammatory Molecules Supriyanto Supriyanto; Simon Bambang Widjanarko; Muhaimin Rifa'i; Yunianta Yunianta
Journal of Tropical Life Science Vol. 11 No. 3 (2021)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.11.03.07

Abstract

Neem plant is rich in bioactive constituents, which make it massively discussed the treatment of various diseases. A study on the immunomodulatory activities of neem is given here. This current work aimed to investigate the effects of neem leaf extract on immunocompetent cells. In vivo experiment was carried out using mice (Mus musculus)  induced with DMBA, comprising positive control, negative control, and treatments of neem leaf extracts (250, 500, and 1000 ppm). Data obtained from flow cytometric analysis were evaluated using BD Cellquest ProTM software, then statistically analyzed in SPSS version 21. Parametric analysis in one-way ANOVA was performed at a significance level of 5%. The significant difference was compared in the Duncan test. The results showed that administration of neem leaf extracts significantly affected the expression of CD4+, CD8+, CD25+, CD62L, IL-10, and IL-17 cells .Neem leaf extract has immunomodulatory activities by increasing pressure molecules and decreasing pro-inflammatory molecules
Isolation And Identification of Cyanobacteria from Selected Caves of Bulabog Putian National Park Dingle, Iloilo, Western Visayas, Philippines Mary Lou Crucero Arabaca; Noel D. Roble; Jenelle Mae B. Sanchez
Journal of Tropical Life Science Vol. 12 No. 1 (2022)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.12.01.09

Abstract

Cyanobacteria are photosynthetic, gram negative, and diverse group of prokaryotic organisms, inhabiting a wide range of habitats including caves. This study was aimed to isolate and identify cyanobacteria in selected caves of Bulabog Putian National Park, Dingle, Iloilo, Western Visayas, Philippines. Based on morphology and 16S rRNA gene sequence analysis six strains of cyanobacteria were identified. The sequence homology search by BLAST program revealed that the closest relative of the six strains was Leptolyngbya sp. (similarity - 94.62 Calothrix sp. (similarity – 97.07%), Chroococcidiopsis cubana (similarity – 99.21%), Onodrimia javanensis (similarity – 96.27%), Chroakolemma pellucida (similarity – 94.74%), and Albertania skiophila (similarity – 98.40%), belonging to Order Chroococcidiopsales, Nostocales, and Synechococcales. The phylogenetic tree revealed the taxonomic positions of the six cyanobacterial strains. Based on the results, six newly identified strains of cyanobacteria have been discovered from the caves of Bulabog Putian National Park. Further studies such as the polyphasic approach and other taxonomic characterizations are needed for the description of these new novel taxa of cyanobacteria.
Ethnobotanical Study and Conservation Status of Plants Used by the Tigwahanon-Manobo in Mt. Malimumu, San Fernando, Bukidnon, Philippines: Ethnobotanical study and conservation status of plants used by the Tigwahanon-Manobo Mark Lylod G. Dapar; Victor B Amoroso
Journal of Tropical Life Science Vol. 12 No. 2 (2022)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.12.02.02

Abstract

Central Mindanao, Philippines, is a center of the cultural wealth of knowledge andbotanical resources in the southern archipelago. However, traditional plant resources remain unexplored, and most of the tribal communities remain undocumented. Manobo tribe is one of the most populated and diverse tribal communitiesin the country, including the secluded Tigwahanon-Manobo community in Mt.Malimumu, San Fernando, Bukidnon, Philippines. Hence, this study provides ethnobotanical information and conservation status of plants along a 1-km transectwalk in Mt. Malimumu used by the Tigwahanon-Manobo. A guided field walkwith the tribal chieftains and tribal healer documented a total of 23 species in 22genera belonging to 20 families, of which four species, namely Dillenia philippinensis Rolfe, Nepenthes cornuta Marwinski, Coritico, Wistuba, Micheler,Gronem., Gieray & V.B.Amoroso, Nepenthes truncata Macfarl., and Pandanusmindanaensis Martelli, are Philippine endemics. Three families, namely Nepenthaceae, Melastomataceae, and Rubiaceae, are the most represented with two species each. Based on the combined international (IUCN 2021) and national (DENRDAO 2017-11) listing of conservation status, one species was assessed as Critically Endangered (Aquilaria malaccensis Lam.) and another one as Endangered(N. truncata). Two species were assessed as Vulnerable (Agathis philippinensisWarb. and N. cornuta) and one species as Near Threatened (D. philippinensis).Six species were identified as Least Concern while the rest of the species were notyet evaluated. This participatory research provides the first ethnobotanical studyand conservation status of traditional plants used by Tigwahanon-Manobo for foodand medicine, as well as economic and ecological uses needing conservation andprotection of their natural resources.Keywords: Conservation, Ethnobotany, Food plants, Indigenous, Medicinalplants, Mindanao
Wintering of Leaf Warblers (Phylloscopus Boie, 1826) in Gangajalghati: First photographic evidence of four species from Northern Bankura, West Bengal, India. Ananya Nayak
Journal of Tropical Life Science Vol. 12 No. 1 (2022)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.12.01.13

Abstract

Leaf warblers are small insectivorous passerine birds that belong to the genus Phylloscopus (Boie, 1826) and exhibit great similarity in plumage and morphology. These song birds have been reported with the maximum diversity in the eastern Himalayas and southern China. No comprehensive studies with photographic evidence on Phylloscopus have been conducted so far in the Southern parts of West Bengal including Bankura district in India. Three types of habitats of Gangajalghati village in Bankura district were surveyed during winter for 8 months between November 2015 and February 2017 and bird counts were recorded and photographed. A total of 49 individuals from four species of Phylloscopus were recorded. The most abundant species recorded in the study was the dusky warbler (Phylloscopus fuscatus, Blyth, 1842) followed by greenish warbler (Phylloscopus trochiloides, Sundevall, 1837), common chiffchaff (Phylloscopus collybita, Vieillot, 1817) and Hume's leaf warbler (Phylloscopus humei, Brooks, 1878). Further, the prey abundance of leaf warblers (lepidopteran caterpillar and other arthropods) were determined in the early winter session (first week of November and December). The study recorded maximum bird abundance and species richness in the wetland associated habitats with higher prey abundance followed by barren land and agriculture field. The work documented the visit of dusky and greenish warblers to Bankura for the first time in the past 40 years and recorded the wintering of common chiffchaff and Hume's leaf warblers as the first evidence from the district.

Page 72 of 82 | Total Record : 815

 70   71   72   73   74 

Filter by Year

2010 2023


Reset
Filter By Issues
All Issue Vol. 13 No. 3 (2023) Vol. 13 No. 2 (2023) Vol. 13 No. 1 (2023) Vol. 12 No. 3 (2022) Vol. 12 No. 2 (2022) Vol. 12 No. 1 (2022) Vol 11, No 3 (2021) Vol. 11 No. 3 (2021) Vol. 11 No. 2 (2021) Vol 11, No 2 (2021) Vol 11, No 1 (2021) Vol. 11 No. 1 (2021) Vol. 10 No. 3 (2020) Vol 10, No 3 (2020) Vol. 10 No. 2 (2020) Vol 10, No 2 (2020) Vol 10, No 1 (2020) Vol. 10 No. 1 (2020) Vol. 9 No. 3 (2019) Vol 9, No 3 (2019) Vol. 9 No. 2 (2019) Vol 9, No 2 (2019) Vol 9, No 1 (2019) Vol. 9 No. 1 (2019) Vol 8, No 3 (2018) Vol. 8 No. 3 (2018) Vol 8, No 2 (2018) Vol. 8 No. 2 (2018) Vol 8, No 1 (2018) Vol. 8 No. 1 (2018) Vol 7, No 3 (2017) Vol. 7 No. 3 (2017) Vol. 7 No. 2 (2017) Vol 7, No 2 (2017) Vol. 7 No. 1 (2017) Vol 7, No 1 (2017) Vol 6, No 3 (2016) Vol. 6 No. 3 (2016) Vol. 6 No. 2 (2016) Vol 6, No 2 (2016) Vol. 6 No. 1 (2016) Vol 6, No 1 (2016) Vol 5, No 3 (2015) Vol. 5 No. 3 (2015) Vol 5, No 2 (2015) Vol. 5 No. 2 (2015) Vol. 5 No. 1 (2015) Vol 5, No 1 (2015) Vol 4, No 3 (2014) Vol. 4 No. 3 (2014) Vol. 4 No. 2 (2014) Vol 4, No 2 (2014) Vol 4, No 1 (2014) Vol. 4 No. 1 (2014) Vol 3, No 3 (2013) Vol. 3 No. 3 (2013) Vol 3, No 2 (2013) Vol. 3 No. 2 (2013) Vol 3, No 1 (2013) Vol. 3 No. 1 (2013) Vol. 2 No. 3 (2012) Vol 2, No 3 (2012) Vol. 2 No. 2 (2012) Vol 2, No 2 (2012) Vol 2, No 1 (2012) Vol. 2 No. 1 (2012) Vol 1, No 2 (2011) Vol. 1 No. 2 (2011) Vol. 1 No. 1 (2010) Vol 1, No 1 (2010) More Issue