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INDONESIA
Journal of Agricultural Science and Biotechnology
Published by Universitas Udayana
ISSN : 23020113     EISSN : -     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Mempublikasikan hasil-hasil penelitian yang berbasis bioteknologi dalam pemanfaatannya dibidang pertanian, termasuk biological control, pascapanen, serta semua penelitian tentang pemanfaatan komponen biologi yang dikemas dalam teknologi dalam lingkup pertanian secara luas.
Arjuna Subject : -
Articles 5 Documents
 1 
Search results for , issue "Volume 1, No 1, Tahun 2012" : 5 Documents clear
THE POSSIBLE ROLE OF CELL WALL DEGRADATION ENZYMES TO BACTERIOPHAGES INFECTION AND PRODUCTION (Review) I Putu Sudiarta
Journal of Agricultural Science and Biotechnology Volume 1, No 1, Tahun 2012
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Penggunaan pestisida kimia sintetis untuk mengendalikan hama dan penyakit tumbuhan memiliki banyak pengaruh negatif, seperti ketahanan serangga hama dan penyakit terhadap pestisida, resurgensi, ledakan hama sekunder, punahnyah musuh alami dan residu pada bahan makanan dan lingkungan. Selama 60 tahun-an terakhir tingkat keracunan dibidang pertanian dan lingkungan semakin meningkat secara dramatis. Oleh karena itu salah satu pendekatan untuk mengurangi penggunaan pestisida kimia adalah menggunakan musuh alami. Untuk mengendalikan penyakit yang disebabkan oleh bakteri salah satu pendekatan mutahir menggunakan musuh alami berupa bakteriopag. Bakteriopag menyerang bakteri dengan kisaran inang yang spesifik. Salah satu penelitian yang menarik adalah tentang mekanisme bakteriopag menyerang inangnya. Untuk menghancurkan dinding sel bakteri, bakteriopag menghasilkan banyak ensim pendegradasi dinding sel. Pada kajian ini didiskusikan beberapa ensim pendegradasi dinding sel dan kemungkinan perannannya dalam mekanisme infeksi bakteriopag.
ISOLASI DAN IDENTIFIKASI JAMUR PENGHASIL MIKOTOKSIN PADA BIJI KAKAO KERING YANG DIHASILKAN DI FLORES Emilia Simpllisiu Ake Wangge; Dewa Ngurah Suprapta; Gusti Ngurah Alit Susanta Wirya
Journal of Agricultural Science and Biotechnology Volume 1, No 1, Tahun 2012
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

A study entitled “Isolation and Identification of Mycotoxin-producing Fungi on Cocoa Beans Produced in Flores” was done in order to know the population and species of fungi and to know the concentration of mycotoxin in the dried cocoa beans. The dry cocoa beans used in this study were collected from several locations in Flores. Isolation of the fungi was done using Sabouraud Dextrosa Agar (SDA), while the determination of mycotoxin concentration was done based on Enzym Linked Immunosorbent Assay (ELISA). Results of this study showed that,  there were several fungi were found in the dried cocoa beans produced in Flores, namely Aspergillus niger, A. flavus, A. parasiticus, A. fumigatusandMucor sp. All tested dried cocoa beans contained aflatoxin B1, where the highest aflatoxin B1 concentration was found in the fermented beans from West Manggarai with the concentration at 3.65 ppb, while the lowest concentration was 2.21ppb, which was found in the cocoa beans from Nagekeo. The highest concentration of ochratoxin A was 0.38  ppb, which was found in the cocoa beans from West Manggarai, while no ochratoxin A was detected in the cocoa beans from Ende.
PENGARUH DRY HEAT TREATMENT TERHADAP DAYA SIMPAN BENIH CABAI RAWIT (Capsicum frutescens L.) I Gusti Ngurah Raka; Anak Agung Made Astiningsih; I Dewa Nyoman Nyana; I Ketut Siadi
Journal of Agricultural Science and Biotechnology Volume 1, No 1, Tahun 2012
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

The aims of this experiment were to compare the shelf life of chili pepper seeds given dry heat treatment (DHT) and non dry heat treatment (NT). This experiment was conducted in Laboratory of Seed Technology Faculty of Agriculture, Udayana University. The study consisted of two experiments that attempt to use lots of chili seeds treated with dry heat treatment (DHT) and experimental use a lot of chili seeds without dry heat treatment (NT). This experiment uses a randomized completely design (RCD) with 10 replications.  Each type of chili pepper seeds tested each power shelf with 10 replications. Storage treatment performed at room temperature, which consists of non-storage (T0), storage for 2 weeks (T2), storage for 4 weeks (T4), storage for 6 weeks (T6) and storage for 8 weeks (T8). Thus, for each lots of chili pepper there are 50 experimental units. Treatment until 8 weeks of storage time did not affect the viability and vigor of pepper seeds either DHT or NT with value up to 80%, so they do not affect seed longivity.  The dry heat treatment (DHT) showed the values of seed and seedling vigor are higher compared to that of no dry heat treatment (NT).
EFIKASI MINYAK ATSIRI TANAMAN CENGKEH (Syzygium aromaticum (L.) Meer. & Perry), PALA (Myristica fragrans Houtt), DAN JAHE (Zingiber officinale Rosc.) TERHADAP MORTALITAS ULAT BULU GEMPINIS DARI FAMILI LYMANTRIIDAE Made Mika Mega Astuthi; Ketut Sumiartha; I Wayan Susila; Gusti Ngurah Alit Susanta Wirya; I Putu Sudiarta
Journal of Agricultural Science and Biotechnology Volume 1, No 1, Tahun 2012
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

The analysis of efficacy of clove oil (Syzigium aromaticum), ginger oil (Zingiber officinale), and nutmeg oil (Myristica fragrans) to hairy caterpillar was conducted. The hairy caterpillar were reported to attack some plants in 2010 to 2011 in Indonesia .To control the caterpillar, recently, peoples used chemical insecticide, however the impact of chemicals insecticide is dangerous to human being, livestock, and environmental. Therefore to minimizing those problems, the control methods should be environmental-friendly and safe against human being. One of those methods is utilizing the botanical pesticide which is extracted from tropical plants. Therefore efficacy of essential oils was done in order to find out the method to control population of it with environmental friendly approach. The experiment result shown at the concentration 10%, all of the essential oils are effective to kill the caterpillar (90-100%). Therefore the examinations of low concentrations of essential oils were conducted (5, 2, and 1%). The result of 1% concentration of ginger oil, nutmeg oil and clove oil are 80 %, 76 % and 68 % respectively.
UTILIZATION OF SDT-RT-PCR FOR PLANT VIRUS DETECTION I Gede Rai Maya Temaja; Ni Made Puspawati; Ni Nyoman Ari Mayadewi
Journal of Agricultural Science and Biotechnology Volume 1, No 1, Tahun 2012
Publisher : Journal of Agricultural Science and Biotechnology

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Abstract

Metode simple-direct-tube (SDT) dikembangkan untuk preparasi     RNA virus atau viroid, yang selanjutnya dipakai untuk sintesis cDNA. SDT tidak memerlukan penyaringan atau sentrifugasi seperi pada ekstraksi RNA total menggunakan KIT. Metode ini juga tidak menggunakan antiserum dalam preparasi RNA virus. Di samping itu metode SDT dapat dikerjakan di laboratorium dengan temperatur ruang dan selesai dikerjakan lebih kurang dalam 25 menit. Penerapan metode ini dilanjutkan dengan reverse transcription-polymerase chain reaction (RT-PCR), dapat mendeteksi keberadaan tomato chlorosis virus (ToCV), tomato infectious chlorosis virus (TICV), chrysanthemum virus B (CVB), Potyvirus and chrysanthemum stunt viroid (CSVd). Hal ini menunjukkan bahwa metode SDT dapat dipakai untuk mendeteksi virus atau viroid.

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