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INDONESIA
JURNAL BIOLOGI INDONESIA
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : 08544425     EISSN : 2338834X     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 16 Documents
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Search results for , issue " Vol 8, No 2 (2012): JURNAL BIOLOGI INDONESIA" : 16 Documents clear
Analisis Kariotipe Ular Trawang (Coelognathus radiatus, (Boie 1827)) Qurniawan, Tony Febri; Arisuryanti, Tuty; Nur Handayani, Niken Satuti
JURNAL BIOLOGI INDONESIA Vol 8, No 2 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v8i2.3047

Abstract

Analysis of Radiated Ratsnakes (Coelognathus radiatus, (Boie 1827) Karyotype. Radiatedratsnake is a reptile which has an economic potential an export comodyty. However, there arefew reports on genetic studies of radiated ratsnake. The aim of this research was to examinechromosome characters of the radiated ratsnake collected from Sewon, Bantul, Daerah IstimewaYogyakarta. Chromosome preparation method used was splash using blood culture. The resultsrevealed that the diploid chromosome number (2n) of radiated ratsnake was 30, classified asmetacentric (chromosome pairs number 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, dan 11), submetacentric(chromosome pairs number 12 and 13), and telocentric (chromosome pairs number 14 and 15).Therefore the karyotype formula of radiated ratsnake was 2n = 2x = 30 = 22 m + 4 sm + 4 t. Theresult showed that the shortest of short arm chromosomes was 0 ?m, the longest one was0.665 ± 0.0504 ?m, the shortest of long arm chromosomes was 0.268 ± 0.0011?m, the longestone was 0.746 ± 0.0059 ?m, the shortest of total length chromosomes was 0.27 ± 0.001 ?m, andthe longest of total length chromosomes was 1.41 ± 0.045 ?m. The R value was 5.267 ± 0.15408indicating that radiated ratsnake from Sewon, Bantul, Daerah Istimewa Yogyakarta haschromosome variation size.Keywords: Coelognathus radiatus, blood culture, chromosome character, karyotype
Performa Perangkat Diagnostik Elisa Toksoplasmosis pada Serum Domba dan Manusia Subekti, Didik T.; Hayati, Lisda; Raharja, Sujud M.
JURNAL BIOLOGI INDONESIA Vol 8, No 2 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v8i2.3052

Abstract

Performance of ELISA Toxoplasmosis Diagnostic Kits on Sheep and Human Sera Toxoplasma seropositivity in Indonesia have a high prevalence, both in human and animals. Unfortunately, the availability of diagnostic tools to support dynamic surveillance are limited. Recently, the diagnostic tools for toxoplasmosis, namely ELISA BM were developed. The technology was based on ELISA technique using soluble tachyzoite antigen from tachyzoites of Toxoplasma gondii. Kit performance is one of the important issue for acceptance of diagnostic tools prior to wide application. The purpose of the studies was to asses the quality of diagnostic toolsperformances. The assesment comprises of four stages. First stage was to evaluate the performance of ELISA BM compared to Latex Agglutination Test (LAT) on sheep sera. Secondly, to evaluate the performance of ELISA BM to descriminate true seropositive andseronegative toxoplasmosis on human sera. The last stage were comparing ELISA BM, ELISA TL (commercial kit) and LAT on predetermined and unknown human sera. The results showthat the accuracy of ELISA BM is slightly better than ELISA TL. Agreement of ELISA BM with LAT was better againts ELISA TL with LAT. However, all performance as determined using Cohen’s ? and Gwet’s AC1 of ELISA BM, ELISA TL and LAT were good up to very good agreement.Keywords: Toxoplasmosis, ELISA, Latex Agglutination, inter reliability agreement.
Komunitas Kodok pada Lahan Terdegradasi di Areal Curug Nangka, Jawa Barat Kurniati, Hellen; Sumadijaya, Alex
JURNAL BIOLOGI INDONESIA Vol 8, No 2 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v8i2.3043

Abstract

Frogs communities of degraded areas in Curug Nangka area, West Java. A Total of thirteenfrog species were found in degraded land areas of Curug Nangka, on the foot hills of MountSalak at elevation of 640 m - 730 m above sea level (asl). In the fast-flowing river habitat, threefrog species dominated this habitat, they were Huia masonii, Hylarana chalconota andOdorrana hosii; whereas in fast-flowing ditch habitat, Phrynoidis aspera and H. chalconotawere the dominant species, and for the pine plantation, H. chalconota and H. nicobariensisdominated this habitat. On the river transect, the presence of individuals H. masonii wasinfluenced by the dynamics of air temperature and water temperature. In the open ditchtransects, the presence of individual P. aspera was influenced by water temperature. Therewas a stronger positive association between the presence of individuals H. masonii andindividuals of O. hosii than between individual O. hosii and H. chalconota on the rivertransect. Among individuals of H. masonii and individuals of H. chalconota there was apositive association; however in the open ditch transects, there was no association betweenthe presence P. aspera and H. chalconota. The presence of H. chalconota and H. nicobariensison the pine plantation transect had strong positive association.Keywords: frog community, degraded area.
Pengaruh Habitat dan Ketinggian Tempat Terhadap Sebaran Kelelawar di Taman Nasional Gunung Ciremai, Jawa Barat Maharadatunkamsi, Maharadatunkamsi
JURNAL BIOLOGI INDONESIA Vol 8, No 2 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (76.822 KB) | DOI: 10.14203/jbi.v8i2.3057

Abstract

Effect of habitat and altitudinal changes on the distribution of bats in Gunung Ciremai National Park, West Jawa. We investigated the bat community in its density and diversity between primary forest, secondary forest, shrub and pines in Gunung Ciremai National Park. This paper discuss the impact of these nature changes on the bat community along this national park. Our data showed a relationship between distribution of each bat species with habitat changes andaltitudinal gradients between 500-600 and 1100-2100 m above sea level. The distribution of bats according to their habitat and altitude in this study is parallel with some other previous studies. The results indicated that both habitat and altitidinal differences are important factors in influencing the distribution of each bat species. Although forest and disturbed areas provide food and shelter for support many of these bats species, it is urgent to maintain and improve the quality of the forests in this important national park.Keywords: Gunung Ciremai, bats, forests, disturbed areas, altitudinal.
Pengaruh Struktur dan Komposisi Vegetasi dalam Menentukan Nilai Konservasi Kawasan Rehabilitasi di Hutan Wanagama I dan Sekitarnya Purnomo, Danang Wahyu; Usmadi, Didi
JURNAL BIOLOGI INDONESIA Vol 8, No 2 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v8i2.3048

Abstract

The Effectof Vegetation Structure and Composition in Determining the Conservation Value of Rehabilitation Area in the Wanagama I Forest and Surroundings. Wanagama I Forest has many types of land use that distinguished by a combination of agroforestry systems. The aim of the research is to determine the conservation value based on the bird diversity in each of the land use types and to understand the effect of vegetation structure and composition on bird diversity. The conservation value was measured using two factors, namely diversity index (H’) and conservation value index (CVI). Bird diversity was estimated using point counts method,while vegetation structure and composition was observed using systematic nested sampling. The highest H’ and CVI were found at the old growth forest (H’= 2.328, CVI = 2.556) while thelowest were detected at the bush (H’ = 1.607, CVI = 1.941). Rehabilitation effort significantly increased bird diversity. In accordance, vegetation structure and composition i.e. cover density,the number of species, dominance, and canopy strata, significantly affected the bird diversity (F = 2.979, P = 0.005). However, canopy strata (B = 0.20, P = 0.001) and density of vegetation atsapling level (B = 0.12, P = 0.036) were the most significant factors. Dominant species such as Gliricidea sepium and Tectona grandis were causative factors that attracted the olive-backedsunbird (Nectarinia jugularis) and insectivorous (Pycnonotus aurigaster and Orthotomus sepium).Keywords: Conservation index, diversity index, density, dominance, composition and structure, Wanagama I Forest
Pengujian Ketahanan Kekeringan pada Tanaman Garut (Maranta arundinacea L.) Hasil Mutasi Dengan Radiasi Sinar Gamma Hidayati, Nuril; Sukamto, Lazarus A.; Juhaeti, Titi
JURNAL BIOLOGI INDONESIA Vol 8, No 2 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (201.008 KB) | DOI: 10.14203/jbi.v8i2.3053

Abstract

Drought Tolerance Assay on Resulted Mutation of Arrowroot Plant (Maranta arundinacea L.) with Gamma Irradiation. Selection of garut (Maranta arundinacea L.) toward drought stress was conducted in induced mutant by using provenance plants from some semi aridregions of East Jawa. In this research three provenance were used 1) Garut from Dusun Pogal, Desa Lebakrejo, Kec. Purwodadi, Kab. Pasuruan (N1); 2) Garut from Dusun Sembung, Desa Parerejo, Kec. Purwodadi, Kab. Pasuruan (N5); 3) Garut from Dusun Genitri, Desa Gunting, Kec. Sukorejo, Kab. Pasuruan (N8). Provenance plants were treated with mutation induction using several levels of gamma radiation i.e. 0, 10, 20 and 40 Gy. The inducted plants were thenplanted in optimum environmental condition for acclimatization. After 5 months the plants were placed in a greenhouse for water stress treatments. Three levels of water regimes 1) optimum water (field capacity Ø = -0,3 to -1,5 Mpa); 2) 7 days watering interval (Ø =-1,0 to -11,0 Mpa); 3) 14 days interval (Ø = -4,0 to -15,0 Mpa). Plant drought tolerance was examined by analyzing morphological and physiological characteristics related to drought tolerant characteristics, including stomatal conductance (stomatal opening), transpiration, rate of CO2 assimilation, biomass production and yield, Harvest Index and drought Tolerance Index. The resultsshowed that radiation treated plants were more capable of maintaining their water potential (Ø). This indicated by significantly higher values of Ø in treated plants i.e. -2.95 Mpa(10 Gy),-2.86 Mpa (20 Gy) and -2.84 Mpa (40 Gy), compared to -3.74 (Untreated plants). Drought stressed plants produced total biomass 79,55 g/plant, much lower compared to unstressed plants (308,20 g/plant). The highest yield was N8 (219,53 g biomass and 139,83 g tuber), followed by N1 (183,32 g biomass and 126,20 g tuber) and N5 (178,8 g biomass and 136,64 g tuber). Drought Tolerance Index of untreated N1 was the highest (1,27), followed by N5 treatedwith 40 Gy (1.22), N1 with 10 Gy (1.17) and N8 with 40 Gy (1.00). Among radiation treatments, untreated plant produced the highest yield followed by the plants treated with 10 Gy, and the lowest was treated with 40 Gy.Keywords: Drought, tolerance, Maranta arundinacea, mutation, gamma, radiation
Perbanyakan Massal Anthurium daun (Anthurium sp) asal biji dengan Teknologi In Vitro Witjaksono, Witjaksono
JURNAL BIOLOGI INDONESIA Vol 8, No 2 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (182.259 KB) | DOI: 10.14203/jbi.v8i2.3058

Abstract

Mass propagation of leafy Anthurium (Anthurium spp.) from seed by in vitro technology. We have developed an efficient in vitro propagation of A. plowmanii from seed explants. The protocols include inoculation of seed in MS medium supplemented with 2 g/l BA. This medium is used to proliferate cluster of buds that developed from seeds. To induce the growth of the shoots from the bud clusters, the clusters were devided and transffered to MS medium enriched with 340 mg/l KH2PO4 and 0,1 mg/l BA and 0,05 mg/l NAA. To get high survival rate, the clusterof shoots were acclimatized as a whole cluster that in turn the shoots become easily separatable from each other during the growth. The acclimatized plantlets grew into mature plants through serial transfer to bigger pots and regular fertilizer application. This technology is applicable to other leafy anthuriums as well.Key words: leafy Anthurium, in vitro technology, BA, NAA, KH2PO4, plowmanii, hookeri, jenmanii, longilinguum
Pertumbuhan Bakteri Hasil Isolasi dari Tanah Perkebunan yang Tumbuh pada Media Mengandung Pestisida Propoksur dan Karbaril Imamuddin, Hartati; Laili, Nur; Rahmansyah, Maman
JURNAL BIOLOGI INDONESIA Vol 8, No 2 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (204.636 KB) | DOI: 10.14203/jbi.v8i2.3054

Abstract

Growth Capacity of Soil Bacteria Isolates Obtained from Estate Area from Media Containing Propoxur and Carbaryl. Isolation actions have been carried out with three soil samples derived from soil estate of pine apple, sugar cane, and banana in Lampung area, Southern Sumatra. The isolates were grown in the selected media containing carbaryl insecticide, and herbicide of diuron and bromocyl. The highest and vigorous growth isolates have gathered from threedifferent soil samples, and subsequently named GGPC, GM, and NTF isolates. Furthermore, those isolates were studied through the growth rate in the media containing propoxur and carbaryl in the nutrient rich liquid (NB) and compared to limited mineral (MM). All of the isolates grew faster against pesticides in the liquid medium of NB compared to MM. High correlated value among parameters nurtured as due to bacterial population and those processes were not affected by media composition. The GM isolate showed similar pattern during propoxur decrease along with incubation even though the rate of pesticide elimination from the media was higher in the rich nutrient liquid media (NB) compared to MM.Keywords: microbe isolate, pesticide, propoxur, carbaryl
Pengaruh Suplementasi Konsentrat dalam Ransum terhadap Performa, Bobot Karkas dan Non Karkas Landak Jawa (Hystrix javanica F. Cuvier, 1823) Sari, Andri Permata; Farida, Wartika Rosa; Tjakradidjaja, Anita S.
JURNAL BIOLOGI INDONESIA Vol 8, No 2 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v8i2.3059

Abstract

The Effect of Concentrate Supplementation in The Ration on Performance, Carcass and Non-Carcass Weight of Javan Porcupine (Hystrix javanica F. Cuvier, 1823). This study was conducted at Small Mammal Captivity, Zoology Division, Research Center for Biology,Indonesian Institute of Sciences, Cibinong, Bogor Regency. Eight porcupines consisted of 6 females and 2 males were used to know the effect of concentrate supplementation in the ration on performance, carcass and non carcass weight of javan porcupine. Ration treatment consisted of control ration (PI) and control ration plus concentrate comersial such as koi fish pellet (PII).Four porcupines (3 female and 1 male) were given control ration (PI) and three porcupines (3 female and 1 male) were given ration PII. A completely randomized design was used in this research. Data collected were nutrient consumption, average daily gain, feed conversion, slaughter weight, weight of cascass and non-carcass. The data were analyzed by analysis of variance. The result showed supplementation of koi fish pellet in the ration didn’t significantlyinfluence (P>0.05) performance of javan porcupine, but significantly (P<0.05) increase consumption on calcium and phosphor. Weight and percentage of carcass and non-carcass of javan porcupine were not affected by ration treatment. Average of weight and percentage of porcupine’s carcass of PI were 4,505.08 g and 59.67% and those of PII were 3,957.55 g and 56.54%, while average of weight and percentage of porcupine’s non-carcass of PI were 2,979.05g and 39.46% and those of PII were 2,774.60 g and 39.64%.Keywords: Concentrate, ration, performace, carcass, non-carcass
Potensi Serapan Karbondioksida (CO2) pada Beberapa Jenis Tumbuhan Lantai Hutan Dari Suku Araceae Di Taman Nasional Gunung Halimun- Salak, Jawa Barat Mansur, Muhammad
JURNAL BIOLOGI INDONESIA Vol 8, No 2 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (238.067 KB) | DOI: 10.14203/jbi.v8i2.3050

Abstract

Potential Absorption of Carbon Dioxide (CO2) in Some Plant species on The Forest Floor From Family of Araceae in The Gunung Halimun-Salak National Park, West Java. Study of carbon dioxide (CO2) absorption in some plant species on the forest floor from Family of Araceae conducted in the Gunung Halimun-Salak National Park, Resort Cidahu, West Java, on September 2011. The survey was conducted to inventory the species of Araceae that exist atthe study site. Thirty samples of the five species of Araceae has been chosen as the target of photosynthesis measurement. Six individuals from each species were measured as replicates,in which young and old leaves were measured from each individual. The results show that five species of Araceae are found in forest floor, namely; Schismatoglottis calyptrata, Schismatoglottis rupestris, Alocasia longiloba, Arisaema filiforme and Colocasia esculenta.Colocasia esculenta has a higher CO2 absorption rate (14, 288 ?mol/m2/s) than Arisaema filiforme (8,614 ?mol/m2/s), Alocasia longiloba (7,599 ?mol/m2/s), Schismatoglottis rupestris(7,078 mol/m2/s) and Schismatoglottis calyptrata (5,260 ?mol/m2/s). In general, old leaves of all species have higher chlorophyll contain and CO2 absorption rate than young leaves. Theoptimum photosynthetic rate in Schismatoglottis calyptrata occurred at 10:00 am (8,457 ?mol/m2/s) and the lowest at 16:00 pm (4,262 ?mol/m2/s).Keywords: CO2 absorption, Araceae, Gunung Halimun-Salak National Park.

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