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Journal : Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH)

THE INHIBITION OF RED CHILIES RIPENING PROCESS AT FRESH WEIGHT AND TOTAL CARBOHYDRATE CONTENT BY RED LIGHT Martha Lulus Lande; Salman Farisi
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 1 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i1.162

Abstract

The research was conducted in a factorial experiment with factor A: far-red light treatment with 2 levels: control (without far-red light), and treatment (far-red light). Factor B was a time of observation with 3 levels: 4, 6, 8 days after wrapping. Wrapping each detached fruit in a combination of four layers of blue-colored plastic, one layer of green-colored plastic, and two layers of red-colored plastic resulted in a red light treatment. Transmission measurement of that plastic combined with a spectrophotometer at wavelength 700-735 nm was 75%. To deter fresh weight, each fruit was weighted with an analytical balance. Total soluble carbohydrate content was determined by the phenol-sulfuric method and counted based on the glucose standard curve (mg/gram tissue). Analysis of variance was conducted at a 5% significant level, and if the interaction was significant, then continued with simple effect determination at a 5% significant level by the F test. The fresh weight average of the soluble carbohydrate content of treated fruits was higher than the control. The fresh weight of chili fruits was relatively constant during observation of both treated and controlled fruits. The total soluble carbohydrate content average relatively did not differ between treated and control fruits. Total soluble carbohydrate content was increased 6 days after treatment and decreased 8 days after treatment. The result of this research concluded that red light inhibited the ripening process of chili fruits by slowing the decrease of fresh weight.
MANUFACTURE OF LIGNINOLYTIC FUNGI INOCULUM Geotrichum sp. WITH SORGUM (Sorghum bicolor) MEDIA AND ITS EFFECT ON THE QUALITY OF BAMBOO LEAF COMPOST (Bambusa sp.) Nadya Febri Harlifia; Bambang Irawan; Salman Farisi; Suratman
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 1 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i1.163

Abstract

Bamboo is a type of plant that grows in Indonesia. So far, people only use the stems and throw away the leaves. As a result, these abundant bamboo leaves become waste. Bamboo leaf waste contains lignin content of 19.8-26.6%, and becomes compost in the long term. The process of composting bamboo leaf litter can be accelerated using an activator such as the inoculum fungi Geotrichum sp. which is ligninolytic so that it can break down lignin complex compounds contained in bamboo leaf waste into simpler compounds. Sorghum contains about 6.5-7.9% fiber. The high fiber content in sorghum seeds can be used as a growth medium for fungal inoculum. The purpose of this study was to determine the effect of sorghum as an inoculum medium for Geotrichum sp. and the effect of inoculum on compost quality. This research was carried out in August - December 2019 at the Microbiology Laboratory of the Biology Department, Faculty of Mathematics and Natural Sciences, Unila. This study used a completely randomized design (CRD) method with 4 treatments, namely Control (Bamboo leaf litter), P1 (Bamboo leaf litter + 1% inoculum), P2 (Bamboo leaf litter + 1.5 % inoculum) and P3 (Bamboo leaf litter). + 2% inoculum) each treatment was repeated 3 times. The variables observed were the number of spores and spore viability using the Colony Forming Unit (CFU) calculation on inoculum and compost. Analysis of the content of C, N, P, and C/N ratio was carried out to determine the quality of the compost. Based on the research results, sorghum can be used as a growth medium for the ligninolytic fungi inoculum Geotrichum sp. with spore viability of 74.18% and the addition of 1.5% inoculum (P2) gave the best results on the quality of bamboo leaf compost (Bambusa sp.)
BROMELAIN WASTE TEA COMPOST INDUCED BY LIGNINOLYTIC INOCULUM OF Trichoderma sp. ON THE GROWTH OF LEAF NUMBER AND CHLOROPHYLL CONTENT OF CHILI (Capsicum annuum L.) Syafira Clarisa Huda; Bambang Irawan; Salman Farisi; Yulianty Yulianty
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 1 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i1.165

Abstract

Pineapple is one kind of fruit that contains a lot of nutrition.  The waste from residual pineapple processing is called bromelain.  Bromelain contains polymer complex in the form of organic sources from nature if it could be decomposed properly.  One way to exploited bromelain is by composting.  Composting is done with the help of fungi of Trichoderma sp. to speed up the decomposition process.  The resulting compost is aerated to increase the nutrients and form a further decomposition process in the soil.  The research was carried out from January till March 2021 in the Microbiology Laboratory Biology, Faculty of Mathematics and Science, University of Lampung.  The parameters of this research are the content of chlorophyll and the number of leaves.  This research used a completely Randomized Design with 7 treatments that are A1 (24 hours of mixed bromelain), A2 (48 hours of mixed bromelain), A3 (72 hours of mixed bromelain), A4 (24 hours of pure bromelain), A5 (48 hours of pure bromelain), A6 (72 hours of pure bromelain), and A7 (control).  Based on this research the value of the highest chlorophyll A on A6 treatment is 0,655.  A value of the highest on chlorophyll B on A6 treatment that 0,429.  The highest total chlorophyll on the A6 treatment is 1,048.  Based on this research the A4 treatment has the highest average number of leaves compared to other treatments.    
APPLICATION OF BROMELAIN LITTER SOLID COMPOST INDUCED BY LIGNINOLITIK Trichoderma sp. FUNGUS TOWARDS NUMBER OF LEAVES AND CHLOROPHYL CONTENT CHILI PLANTS (Capsicum annuum L.) Jihan Haura; Bambang Irawan; Salman Farisi; Yulianty Yulianty
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 8 No. 1 (2021)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v8i1.166

Abstract

Piles of uncontrolled pineapple peel waste have a negative impact that will affect various aspects of life, either directly or indirectly. Therefore, pineapple peel waste that can no longer be processed can be used for making compost. The taste of pineapple can be degraded biologically with the help of enzymes that can be produced by microorganisms. One of the microorganisms that can support the decomposition process is a group of fungi. In this study, the fungus Trichoderma sp. (Bioggp 5) is a ligninolytic fungus. Trichoderma sp. is a saprophytic soil microorganism that attacks natural pathogenic fungi and is beneficial to plants. The purpose of this study was to determine the effect of solid bromelain compost inoculum of ligninolytic fungi (Trichoderma sp.) on leaf number and chlorophyll content of chili plants (Capsicum annum L.) and the best dose of compost that could be used. This study used a completely randomized design (CRD) with 7 treatments, namely T0 (control), T1 (1.4% pure bromelain), T2 (1.7% pure bromelain), T3 (2% pure bromelain), T4 (1, 4% bromelain + leaf litter), T5 (1.7% bromelain + leaf litter), T6 (2% bromelain + leaf litter). The parameters used were the number of leaves and chlorophyll content. And the results obtained from this study were the T3 dose (2% pure bromelain) was the best dose for the number of leaves, while the T2 dose (1.7% pure bromelain) was the best dose for chlorophyll content.
DINAMIKA POPULASI BAKTERI DAN TOTAL ASAM PADA FERMENTASI BEKASAM IKAN PATIN (Pangasius hypopthalmus) Bella Noor Arfianty; Salman Farisi; Christina Nugroho Ekowati
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 4 No. 2 (2017)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v4i2.133

Abstract

Bekasam merupakan produk makanan tradisional hasil fermentasi dari ikan air tawar dengan penambahan nasi dan garam. Selama proses fermentasi, sumber karbohidrat dipecah menjadi gula-gula sederhana, kemudian diubah menjadi alkohol dan asam. Penggunaan jenis ikan pada pembuatan bekasam berpengaruh terhadap jumlah bakteri asam laktat dan kadar asam laktat yang dihasilkan. Penelitian ini bertujuan untuk mengetahui jumlah total bakteri dan bakteri asam laktat serta kadar asam laktat yang dihasilkan selama proses fermentasi bekasam ikan patin siam (Pangasius hypopthalmus). Penelitian ini dilaksanakan pada bulan Januari sampai Februari 2017 di Laboratorium Mikrobiologi, Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Lampung. Penelitian ini menggunakan metode titrimetri untuk menentukan kadar asam laktat dan metode Angka Lempeng Total untuk menentukan jumlah bakteri. Pengamatan dilakukan setiap 2 hari, 4 hari, 6 hari, 7 hari, 8 hari dan 10 hari dengan 2 kali ulangan. Parameter yang diamati meliputi: penghitungan jumlah total bakteri dan bakteri asam laktat , pengukuran kadar asam laktat, pengukuran pH serta uji organoleptik. Hasil penelitian menunjukkan bahwa nilai total bakteri asam laktat meningkat sampai fermentasi hari ke-6, nilai total bakteri mengalami penurunan, total asam tertitrasi meningkat, sedangkan nilai pH menurun.
ISOLATION OF Salmonella typhoid 16s rRNA GENE FRAGMENT BASED ON POLYMERASE CHAIN REACTION (PCR) Salman Farisi; Wawan Abdullah Setiawan; Suratman Umar
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 7 No. 2 (2020)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v7i2.157

Abstract

Iridescent is one of the traditional fish fermentation products that has a sour taste and is widely known in various regions of Indonesia. In its metabolism LAB produces organic acids, hydrogen peroxide, diacetyl, CO2 and bacteriocin. These compounds are antimicrobial compounds that inhibit the growth of pathogenic bacteria and bacterial decomposition. The purpose of this study was to determine the antibacterial activity produced by lactic acid bacteria isolates from catfish (Pangasius hypophthalmus) inhibiting the growth of Escherecia coli. From iridescent obtained 9 isolates that have different characteristics from each other by the morphology of LAB isolate colonies which include colony color, colony shape, colony elevation and colony margin. After production of antibacterial, all isolates showed positive result when antibacterial tested againts Escherecia coli either in acidic condition or neutralized.
Effect of Pyriproxyfen, a Malaria Vector Larvicide, on the Growth and Mortality of Vannamei Shrimp Sriana Putri; Endah Setyaningrum; Salman Farisi
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 10 No. 1 (2023): June Edition
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v10i1.258

Abstract

Anopheles mosquito shared a breeding site with the vannamei shrimp (Litopenaeus vannamei). Anopheles mosquito larvae commonly live in both stagnant fresh and brackish water. The potential breeding habitat of Anopheles mosquito larvae may be rice fields, pools or ponds, swamps, ditches, and slow-current streams. One of the effective strategies to control malaria is to apply chemical insecticides. Pyriproxifen is a larvicide that contains juvenile hormone-like compounds. Juvenile hormone-like compounds can affect mosquito morphogenesis, characterized by the failure of the larvae to develop into pupae. With a completely randomized design, the shrimp fry was divided into 4 groups,  6 repetitions, 20 individuals each. Shrimp fry in each experimental unit was placed and maintained in plastic jars containing 2.5 L of brackish water. The results showed that the insect growth regulator (IGR) with the active ingredient pyriproxyfen at various concentrations had no significant effect on the growth, mortality, and survival of vannamei shrimp fry.  It can be concluded therefore that pyriproxifen larvicide was safe to be used for controlling malaria mosquito vectors in hatcheries and breeding ponds of vannamei shrimp.